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Protein

Sterol O-acyltransferase 1

Gene

Soat1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption.

Catalytic activityi

Acyl-CoA + cholesterol = CoA + cholesterol ester.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei450By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processCholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

Enzyme and pathway databases

BRENDAi2.3.1.26 3474
ReactomeiR-MMU-8964038 LDL clearance

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol O-acyltransferase 1 (EC:2.3.1.26By similarity)
Alternative name(s):
Acyl-coenzyme A:cholesterol acyltransferase 1
Short name:
ACAT-1
Cholesterol acyltransferase 1
Gene namesi
Name:Soat1
Synonyms:Acact
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:104665 Soat1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 140CytoplasmicSequence analysisAdd BLAST140
Transmembranei141 – 159HelicalSequence analysisAdd BLAST19
Topological domaini160 – 181LumenalSequence analysisAdd BLAST22
Transmembranei182 – 199HelicalSequence analysisAdd BLAST18
Topological domaini200 – 224CytoplasmicSequence analysisAdd BLAST25
Transmembranei225 – 243HelicalSequence analysisAdd BLAST19
Topological domaini244 – 246LumenalSequence analysis3
Transmembranei247 – 264HelicalSequence analysisAdd BLAST18
Topological domaini265 – 501CytoplasmicSequence analysisAdd BLAST237
Transmembranei502 – 517HelicalSequence analysisAdd BLAST16
Topological domaini518 – 540LumenalSequence analysisAdd BLAST23

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4464

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002076421 – 540Sterol O-acyltransferase 1Add BLAST540

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2PhosphoserineBy similarity1
Disulfide bondi518 ↔ 536By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiQ61263
MaxQBiQ61263
PaxDbiQ61263
PRIDEiQ61263

PTM databases

iPTMnetiQ61263
PhosphoSitePlusiQ61263
SwissPalmiQ61263

Expressioni

Gene expression databases

BgeeiENSMUSG00000026600 Expressed in 273 organ(s), highest expression level in cumulus cell
CleanExiMM_SOAT1
ExpressionAtlasiQ61263 baseline and differential
GenevisibleiQ61263 MM

Interactioni

Subunit structurei

May form homo- or heterodimers. Interacts with UBIAD1.By similarity

Protein-protein interaction databases

BioGridi203385, 1 interactor
IntActiQ61263, 17 interactors
MINTiQ61263
STRINGi10090.ENSMUSP00000058344

Chemistry databases

BindingDBiQ61263

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0380 Eukaryota
COG5056 LUCA
GeneTreeiENSGT00530000063122
HOGENOMiHOG000020782
HOVERGENiHBG058198
InParanoidiQ61263
KOiK00637
OMAiRLCIPQF
OrthoDBiEOG091G0991
TreeFamiTF105767

Family and domain databases

InterProiView protein in InterPro
IPR004299 MBOAT_fam
IPR014371 Oat_ACAT_DAG_ARE
IPR030687 Sterol_acyltranf_meta
PANTHERiPTHR10408 PTHR10408, 1 hit
PfamiView protein in Pfam
PF03062 MBOAT, 1 hit
PIRSFiPIRSF000439 Oat_ACAT_DAG_ARE, 1 hit
PIRSF500230 Sterol_acyltranf_ACAT, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q61263-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLRNRLSKS GENPEQDEAQ KNFMDTYRNG HITMKQLIAK KRLLAAEAEE
60 70 80 90 100
LKPLFMKEVG CHFDDFVTNL IEKSASLDNG GCALTTFSIL EEMKKNHRAK
110 120 130 140 150
DLRAPPEQGK IFISRQSLLD ELFEVDHIRT IYHMFIALLI LFVLSTIVVD
160 170 180 190 200
YIDEGRLVLE FNLLAYAFGK FPTVIWTWWA MFLSTLSIPY FLFQRWAHGY
210 220 230 240 250
SKSSHPLIYS LVHGLLFLVF QLGVLGFVPT YVVLAYTLPP ASRFILILEQ
260 270 280 290 300
IRLIMKAHSF VRENIPRVLN AAKEKSSKDP LPTVNQYLYF LFAPTLIYRD
310 320 330 340 350
NYPRTPTVRW GYVAMQFLQV FGCLFYVYYI FERLCAPLFR NIKQEPFSAR
360 370 380 390 400
VLVLCVFNSI LPGVLILFLS FFAFLHCWLN AFAEMLRFGD RMFYKDWWNS
410 420 430 440 450
TSYSNYYRTW NVVVHDWLYY YVYKDLLWFF SKRFKSAAML AVFALSAVVH
460 470 480 490 500
EYALAICLSY FYPVLFVLFM FFGMAFNFIV NDSRKRPIWN IMVWASLFLG
510 520 530 540
YGLILCFYSQ EWYARQHCPL KNPTFLDYVR PRTWTCRYVF
Length:540
Mass (Da):63,799
Last modified:July 27, 2011 - v2
Checksum:iF2A6C91774762E23
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WNN8A0A087WNN8_MOUSE
Sterol O-acyltransferase 1
Soat1
220Annotation score:
A0A087WSJ5A0A087WSJ5_MOUSE
Sterol O-acyltransferase 1
Soat1
32Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti195R → P in AAC42075 (PubMed:7592824).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42293 mRNA Translation: AAC42075.1
S81092 mRNA Translation: AAB36050.1
AK142486 mRNA Translation: BAE25081.1
AK159393 mRNA Translation: BAE35047.1
AK159529 mRNA Translation: BAE35158.1
AK160017 mRNA Translation: BAE35563.1
BC083092 mRNA Translation: AAH83092.1
CCDSiCCDS35743.1
PIRiI49454
RefSeqiNP_033256.2, NM_009230.3
UniGeneiMm.28099

Genome annotation databases

EnsembliENSMUST00000051396; ENSMUSP00000058344; ENSMUSG00000026600
ENSMUST00000189661; ENSMUSP00000140721; ENSMUSG00000026600
GeneIDi20652
KEGGimmu:20652
UCSCiuc007dcj.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42293 mRNA Translation: AAC42075.1
S81092 mRNA Translation: AAB36050.1
AK142486 mRNA Translation: BAE25081.1
AK159393 mRNA Translation: BAE35047.1
AK159529 mRNA Translation: BAE35158.1
AK160017 mRNA Translation: BAE35563.1
BC083092 mRNA Translation: AAH83092.1
CCDSiCCDS35743.1
PIRiI49454
RefSeqiNP_033256.2, NM_009230.3
UniGeneiMm.28099

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203385, 1 interactor
IntActiQ61263, 17 interactors
MINTiQ61263
STRINGi10090.ENSMUSP00000058344

Chemistry databases

BindingDBiQ61263
ChEMBLiCHEMBL4464

PTM databases

iPTMnetiQ61263
PhosphoSitePlusiQ61263
SwissPalmiQ61263

Proteomic databases

EPDiQ61263
MaxQBiQ61263
PaxDbiQ61263
PRIDEiQ61263

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051396; ENSMUSP00000058344; ENSMUSG00000026600
ENSMUST00000189661; ENSMUSP00000140721; ENSMUSG00000026600
GeneIDi20652
KEGGimmu:20652
UCSCiuc007dcj.1 mouse

Organism-specific databases

CTDi6646
MGIiMGI:104665 Soat1

Phylogenomic databases

eggNOGiKOG0380 Eukaryota
COG5056 LUCA
GeneTreeiENSGT00530000063122
HOGENOMiHOG000020782
HOVERGENiHBG058198
InParanoidiQ61263
KOiK00637
OMAiRLCIPQF
OrthoDBiEOG091G0991
TreeFamiTF105767

Enzyme and pathway databases

BRENDAi2.3.1.26 3474
ReactomeiR-MMU-8964038 LDL clearance

Miscellaneous databases

ChiTaRSiSoat1 mouse
PROiPR:Q61263
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026600 Expressed in 273 organ(s), highest expression level in cumulus cell
CleanExiMM_SOAT1
ExpressionAtlasiQ61263 baseline and differential
GenevisibleiQ61263 MM

Family and domain databases

InterProiView protein in InterPro
IPR004299 MBOAT_fam
IPR014371 Oat_ACAT_DAG_ARE
IPR030687 Sterol_acyltranf_meta
PANTHERiPTHR10408 PTHR10408, 1 hit
PfamiView protein in Pfam
PF03062 MBOAT, 1 hit
PIRSFiPIRSF000439 Oat_ACAT_DAG_ARE, 1 hit
PIRSF500230 Sterol_acyltranf_ACAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSOAT1_MOUSE
AccessioniPrimary (citable) accession number: Q61263
Secondary accession number(s): Q5XK32, Q64180
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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