UniProtKB - Q61221 (HIF1A_MOUSE)
Hypoxia-inducible factor 1-alpha
Hif1a
Functioni
Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:15225651, PubMed:17981124, PubMed:22009797).
Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed:15225651, PubMed:17981124, PubMed:22009797).
Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed:22009797).
Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:26245371).
Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (By similarity).
By similarity4 PublicationsActivity regulationi
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription activator activity Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- E-box binding Source: BHF-UCL
- enzyme binding Source: MGI
- histone deacetylase binding Source: BHF-UCL
- Hsp90 protein binding Source: MGI
- nuclear receptor binding Source: MGI
- p53 binding Source: MGI
- protein-containing complex binding Source: MGI
- protein domain specific binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- protein kinase binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: UniProtKB
- transcription coactivator binding Source: UniProtKB
- ubiquitin protein ligase binding Source: MGI
GO - Biological processi
- angiogenesis Source: MGI
- axonal transport of mitochondrion Source: UniProtKB
- B-1 B cell homeostasis Source: MGI
- blood vessel development Source: MGI
- blood vessel morphogenesis Source: MGI
- camera-type eye morphogenesis Source: MGI
- cardiac ventricle morphogenesis Source: MGI
- cartilage development Source: MGI
- cell differentiation Source: MGI
- cellular glucose homeostasis Source: UniProtKB
- cellular iron ion homeostasis Source: MGI
- cellular response to hypoxia Source: MGI
- cellular response to interleukin-1 Source: Ensembl
- cellular response to virus Source: MGI
- cerebral cortex development Source: MGI
- collagen metabolic process Source: BHF-UCL
- connective tissue replacement involved in inflammatory response wound healing Source: BHF-UCL
- digestive tract morphogenesis Source: MGI
- dopaminergic neuron differentiation Source: MGI
- elastin metabolic process Source: BHF-UCL
- embryonic hemopoiesis Source: MGI
- embryonic placenta development Source: MGI
- epithelial cell differentiation involved in mammary gland alveolus development Source: MGI
- epithelial to mesenchymal transition Source: BHF-UCL
- glucose homeostasis Source: MGI
- heart looping Source: MGI
- hemoglobin biosynthetic process Source: MGI
- hypoxia-inducible factor-1alpha signaling pathway Source: MGI
- intestinal epithelial cell maturation Source: MGI
- iris morphogenesis Source: MGI
- lactate metabolic process Source: MGI
- lactation Source: MGI
- muscle cell cellular homeostasis Source: MGI
- negative regulation of apoptotic process Source: ParkinsonsUK-UCL
- negative regulation of bone mineralization Source: MGI
- negative regulation of gene expression Source: MGI
- negative regulation of growth Source: MGI
- negative regulation of mesenchymal cell apoptotic process Source: MGI
- negative regulation of neuron apoptotic process Source: MGI
- negative regulation of ossification Source: CACAO
- negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway Source: MGI
- negative regulation of reactive oxygen species metabolic process Source: ParkinsonsUK-UCL
- negative regulation of thymocyte apoptotic process Source: MGI
- negative regulation of TOR signaling Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- negative regulation of vasoconstriction Source: MGI
- neural crest cell migration Source: MGI
- neural fold elevation formation Source: MGI
- outflow tract morphogenesis Source: MGI
- oxygen homeostasis Source: MGI
- positive regulation of angiogenesis Source: MGI
- positive regulation of apoptotic process Source: MGI
- positive regulation of autophagy Source: ParkinsonsUK-UCL
- positive regulation of autophagy of mitochondrion Source: ParkinsonsUK-UCL
- positive regulation of blood vessel endothelial cell migration Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of chemokine-mediated signaling pathway Source: MGI
- positive regulation of cytokine production involved in inflammatory response Source: UniProtKB
- positive regulation of epithelial cell migration Source: BHF-UCL
- positive regulation of erythrocyte differentiation Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of gluconeogenesis Source: MGI
- positive regulation of hormone biosynthetic process Source: MGI
- positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: MGI
- positive regulation of macroautophagy Source: MGI
- positive regulation of neuroblast proliferation Source: MGI
- positive regulation of pri-miRNA transcription by RNA polymerase II Source: MGI
- positive regulation of signaling receptor activity Source: MGI
- positive regulation of smooth muscle cell proliferation Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- positive regulation of transcription from RNA polymerase II promoter in response to hypoxia Source: UniProtKB
- positive regulation of vascular endothelial growth factor production Source: UniProtKB
- positive regulation of vascular endothelial growth factor receptor signaling pathway Source: MGI
- regulation of aerobic respiration Source: ParkinsonsUK-UCL
- regulation of catalytic activity Source: MGI
- regulation of cell population proliferation Source: MGI
- regulation of gene expression Source: ParkinsonsUK-UCL
- regulation of glycolytic process Source: MGI
- regulation of protein neddylation Source: UniProtKB
- regulation of thymocyte apoptotic process Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: MGI
- regulation of transforming growth factor beta2 production Source: MGI
- response to fungicide Source: MGI
- response to hypoxia Source: MGI
- response to iron ion Source: Ensembl
- response to muscle activity Source: MGI
- response to reactive oxygen species Source: UniProtKB
- retina vasculature development in camera-type eye Source: MGI
- signal transduction Source: MGI
- vascular endothelial growth factor production Source: MGI
- vasculature development Source: MGI
- visual learning Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-1234158, Regulation of gene expression by Hypoxia-inducible Factor R-MMU-1234174, Cellular response to hypoxia R-MMU-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-MMU-5689880, Ub-specific processing proteases R-MMU-8857538, PTK6 promotes HIF1A stabilization R-MMU-8951664, Neddylation |
Names & Taxonomyi
Protein namesi | Recommended name: Hypoxia-inducible factor 1-alphaShort name: HIF-1-alpha Short name: HIF1-alpha Alternative name(s): ARNT-interacting protein |
Gene namesi | Name:Hif1a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:106918, Hif1a |
VEuPathDBi | HostDB:ENSMUSG00000021109 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Nucleus
- Nucleus 2 Publications
- Nucleus speckle 1 Publication
Note: Colocalizes with HIF3A isoform 2 in the nucleus and speckles (PubMed:21546903). Cytoplasmic in normoxia, nuclear translocation in response to hypoxia (By similarity).By similarity1 Publication
Cytosol
- cytosol Source: UniProtKB
Nucleus
- nuclear body Source: MGI
- nuclear speck Source: UniProtKB
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
- RNA polymerase II transcription regulator complex Source: MGI
Other locations
- axon cytoplasm Source: GOC
- chromatin Source: MGI
- cytoplasm Source: MGI
- motile cilium Source: MGI
- protein-containing complex Source: MGI
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 170 | R → A: Decreases heterodimer formation with ARNT. Impairs heterodimer formation with ARNT; when associated with D-191. 1 Publication | 1 | |
Mutagenesisi | 191 | V → D: Decreases heterodimer formation with ARNT. Impairs heterodimer formation with ARNT; when associated with A-170. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL6046 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127221 | 1 – 836 | Hypoxia-inducible factor 1-alphaAdd BLAST | 836 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 247 | Phosphoserine; by CK1By similarity | 1 | |
Cross-linki | 391 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 402 | 4-hydroxyprolineBy similarity | 1 | |
Cross-linki | 476 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 545 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 545 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 545 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarityCurated | ||
Cross-linki | 551 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarityCurated | ||
Cross-linki | 560 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarityCurated | ||
Modified residuei | 564 | Phosphoserine; by GSK3-betaBy similarity | 1 | |
Modified residuei | 568 | Phosphothreonine; by GSK3-betaBy similarity | 1 | |
Modified residuei | 577 | 4-hydroxyprolineBy similarity | 1 | |
Modified residuei | 589 | Phosphoserine; by PLK3By similarity | 1 | |
Modified residuei | 602 | Phosphoserine; by GSK3-betaBy similarity | 1 | |
Modified residuei | 668 | Phosphoserine; by PLK3By similarity | 1 | |
Modified residuei | 719 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 810 | S-nitrosocysteineBy similarity | 1 | |
Modified residuei | 813 | (3S)-3-hydroxyasparagineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Hydroxylation, Isopeptide bond, Phosphoprotein, S-nitrosylation, Ubl conjugationProteomic databases
PaxDbi | Q61221 |
PRIDEi | Q61221 |
ProteomicsDBi | 273106 [Q61221-1] 273107 [Q61221-2] |
PTM databases
iPTMneti | Q61221 |
PhosphoSitePlusi | Q61221 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000021109, Expressed in retina and 349 other tissues |
ExpressionAtlasi | Q61221, baseline and differential |
Genevisiblei | Q61221, MM |
Interactioni
Subunit structurei
Interacts with the ARNT; forms a heterodimer that binds core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:26245371).
Interacts with COPS5; the interaction increases the transcriptional activity of HIF1A through increased stability (PubMed:11707426).
Interacts with EP300 (via TAZ-type 1 domains); the interaction is stimulated in response to hypoxia and inhibited by CITED2.
Interacts with CREBBP (via TAZ-type 1 domains).
Interacts with NCOA1, NCOA2, APEX1 and HSP90.
Interacts (hydroxylated within the ODD domain) with VHLL (via beta domain); the interaction, leads to polyubiquitination and subsequent HIF1A proteasomal degradation. During hypoxia, sumoylated HIF1A also binds VHL; the interaction promotes the ubiquitination of HIF1A (By similarity).
Interacts with SENP1; the interaction desumoylates HIF1A resulting in stabilization and activation of transcription (PubMed:17981124).
Interacts (via the ODD domain) with NAA10; the interaction appears not to acetylate HIF1A nor have any affect on protein stability, during hypoxia.
Interacts with RWDD3; the interaction enhances HIF1A sumoylation (By similarity).
Interacts with TSGA10 (PubMed:16777103).
Interacts with HIF3A (PubMed:21546903).
Interacts with RORA (via the DNA binding domain); the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. Interaction with PSMA7 inhibits the transactivation activity of HIF1A under both normoxic and hypoxia-mimicking conditions.
Interacts with USP20.
Interacts with RACK1; promotes HIF1A ubiquitination and proteasome-mediated degradation.
Interacts (via N-terminus) with USP19.
Interacts with SIRT2.
Interacts (deacetylated form) with EGLN1.
Interacts with CBFA2T3.
Interacts with HSP90AA1 and HSP90AB1.
Interacts with DCUN1D1; this interaction increases the interaction between VHL and DCUN1D1.
Interacts with HIF1AN (By similarity).
By similarity5 PublicationsBinary interactionsi
Q61221
Isoform 1 [Q61221-1]
With | #Exp. | IntAct |
---|---|---|
Arnt [P53762] | 5 | EBI-8549331,EBI-78852 |
Tsga10 [Q6NY15] | 2 | EBI-8549331,EBI-8549230 |
GO - Molecular functioni
- enzyme binding Source: MGI
- histone deacetylase binding Source: BHF-UCL
- Hsp90 protein binding Source: MGI
- nuclear receptor binding Source: MGI
- p53 binding Source: MGI
- protein domain specific binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- protein kinase binding Source: MGI
- transcription coactivator binding Source: UniProtKB
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 200304, 25 interactors |
DIPi | DIP-31083N |
IntActi | Q61221, 16 interactors |
MINTi | Q61221 |
STRINGi | 10090.ENSMUSP00000021530 |
Chemistry databases
BindingDBi | Q61221 |
Miscellaneous databases
RNActi | Q61221, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 70 | bHLHPROSITE-ProRule annotationAdd BLAST | 54 | |
Domaini | 80 – 155 | PAS 1PROSITE-ProRule annotationAdd BLAST | 76 | |
Domaini | 228 – 298 | PAS 2PROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 302 – 345 | PACAdd BLAST | 44 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 401 | Interaction with TSGA101 PublicationAdd BLAST | 401 | |
Regioni | 1 – 30 | DisorderedSequence analysisAdd BLAST | 30 | |
Regioni | 21 – 30 | DNA-binding1 Publication | 10 | |
Regioni | 170 – 191 | Required for heterodimer formation with ARNT1 PublicationAdd BLAST | 22 | |
Regioni | 380 – 417 | N-terminal VHL recognition siteAdd BLAST | 38 | |
Regioni | 401 – 613 | ODDAdd BLAST | 213 | |
Regioni | 492 – 511 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 544 – 588 | NTADAdd BLAST | 45 | |
Regioni | 569 – 585 | C-terminal VHL recognition siteAdd BLAST | 17 | |
Regioni | 589 – 795 | IDAdd BLAST | 207 | |
Regioni | 593 – 684 | DisorderedSequence analysisAdd BLAST | 92 | |
Regioni | 707 – 734 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 796 – 836 | CTADAdd BLAST | 41 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 728 – 731 | Nuclear localization signalSequence analysis | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 593 – 633 | Polar residuesSequence analysisAdd BLAST | 41 | |
Compositional biasi | 649 – 678 | Polar residuesSequence analysisAdd BLAST | 30 | |
Compositional biasi | 707 – 725 | Polar residuesSequence analysisAdd BLAST | 19 |
Domaini
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3558, Eukaryota |
GeneTreei | ENSGT00940000156774 |
InParanoidi | Q61221 |
OMAi | TQMQEPA |
OrthoDBi | 547545at2759 |
PhylomeDBi | Q61221 |
TreeFami | TF317772 |
Family and domain databases
CDDi | cd00130, PAS, 2 hits |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR001321, HIF-1_alpha IPR014887, HIF-1_TAD_C IPR021537, HIF_alpha_subunit IPR036638, HLH_DNA-bd_sf IPR001610, PAC IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR013655, PAS_fold_3 |
Pfami | View protein in Pfam PF11413, HIF-1, 1 hit PF08778, HIF-1a_CTAD, 1 hit PF00989, PAS, 1 hit PF08447, PAS_3, 1 hit |
PRINTSi | PR01080, HYPOXIAIF1A |
SMARTi | View protein in SMART SM00353, HLH, 1 hit SM00086, PAC, 1 hit SM00091, PAS, 2 hits |
SUPFAMi | SSF47459, SSF47459, 1 hit SSF55785, SSF55785, 2 hits |
TIGRFAMsi | TIGR00229, sensory_box, 2 hits |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS50112, PAS, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEGAGGENEK KKMSSERRKE KSRDAARSRR SKESEVFYEL AHQLPLPHNV
60 70 80 90 100
SSHLDKASVM RLTISYLRVR KLLDAGGLDS EDEMKAQMDC FYLKALDGFV
110 120 130 140 150
MVLTDDGDMV YISDNVNKYM GLTQFELTGH SVFDFTHPCD HEEMREMLTH
160 170 180 190 200
RNGPVRKGKE LNTQRSFFLR MKCTLTSRGR TMNIKSATWK VLHCTGHIHV
210 220 230 240 250
YDTNSNQPQC GYKKPPMTCL VLICEPIPHP SNIEIPLDSK TFLSRHSLDM
260 270 280 290 300
KFSYCDERIT ELMGYEPEEL LGRSIYEYYH ALDSDHLTKT HHDMFTKGQV
310 320 330 340 350
TTGQYRMLAK RGGYVWVETQ ATVIYNTKNS QPQCIVCVNY VVSGIIQHDL
360 370 380 390 400
IFSLQQTESV LKPVESSDMK MTQLFTKVES EDTSCLFDKL KKEPDALTLL
410 420 430 440 450
APAAGDTIIS LDFGSDDTET EDQQLEDVPL YNDVMFPSSN EKLNINLAMS
460 470 480 490 500
PLPSSETPKP LRSSADPALN QEVALKLESS PESLGLSFTM PQIQDQPASP
510 520 530 540 550
SDGSTRQSSP ERLLQENVNT PNFSQPNSPS EYCFDVDSDM VNVFKLELVE
560 570 580 590 600
KLFAEDTEAK NPFSTQDTDL DLEMLAPYIP MDDDFQLRSF DQLSPLESNS
610 620 630 640 650
PSPPSMSTVT GFQQTQLQKP TITATATTTA TTDESKTETK DNKEDIKILI
660 670 680 690 700
ASPSSTQVPQ ETTTAKASAY SGTHSRTASP DRAGKRVIEQ TDKAHPRSLN
710 720 730 740 750
LSATLNQRNT VPEEELNPKT IASQNAQRKR KMEHDGSLFQ AAGIGTLLQQ
760 770 780 790 800
PGDCAPTMSL SWKRVKGFIS SEQNGTEQKT IILIPSDLAC RLLGQSMDES
810 820 830
GLPQLTSYDC EVNAPIQGSR NLLQGEELLR ALDQVN
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4J1E9 | A0A0R4J1E9_MOUSE | Hypoxia-inducible factor 1-alpha | Hif1a | 810 | Annotation score: | ||
A0A0R4J1F0 | A0A0R4J1F0_MOUSE | Hypoxia-inducible factor 1-alpha | Hif1a | 258 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 12 | K → NR in BAC28578 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 31 | S → T in AAC52730 (PubMed:8702901).Curated | 1 | |
Sequence conflicti | 128 | T → A in AAC52730 (PubMed:8702901).Curated | 1 | |
Sequence conflicti | 128 | T → A in CAA64833 (PubMed:8660378).Curated | 1 | |
Sequence conflicti | 351 | I → L in AAC52730 (PubMed:8702901).Curated | 1 | |
Sequence conflicti | 369 | M → K in AAH26139 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 382 | D → A in AAH26139 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 397 | L → H in BAC28578 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 569 | D → G in BAC28578 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 660 | Q → K in BAC28305 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 700 | N → K in AAC52730 (PubMed:8702901).Curated | 1 | |
Sequence conflicti | 700 | N → K in AAC53455 (PubMed:9368100).Curated | 1 | |
Sequence conflicti | 700 | N → K in AAC53461 (PubMed:9368100).Curated | 1 | |
Sequence conflicti | 799 | E → V in CAA64833 (PubMed:8660378).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_007739 | 512 – 525 | Missing in isoform 2. 1 PublicationAdd BLAST | 14 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U59496 Genomic DNA Translation: AAC52730.1 AF003695 mRNA Translation: AAC53455.1 AF004155 , AF004141, AF004142, AF004143, AF004144, AF004145, AF004146, AF004147, AF004148, AF004149, AF004150, AF004151, AF004152, AF004153, AF004154 Genomic DNA Translation: AAC53461.1 Y09085 Genomic DNA Translation: CAA70305.1 Y09085, Y13656 Genomic DNA Translation: CAA70306.1 AK034087 mRNA Translation: BAC28578.1 AK076395 mRNA Translation: BAC36320.1 AK033471 mRNA Translation: BAC28305.1 AK017853 mRNA Translation: BAB30975.1 BC026139 mRNA Translation: AAH26139.1 X95580 mRNA Translation: CAA64833.1 X95002 mRNA Translation: CAA64458.1 |
CCDSi | CCDS25977.1 [Q61221-1] |
PIRi | JC4837 |
RefSeqi | NP_001300848.1, NM_001313919.1 NP_001300849.1, NM_001313920.1 NP_034561.2, NM_010431.2 [Q61221-1] XP_017170450.1, XM_017314961.1 [Q61221-2] |
Genome annotation databases
Ensembli | ENSMUST00000021530; ENSMUSP00000021530; ENSMUSG00000021109 [Q61221-1] |
GeneIDi | 15251 |
KEGGi | mmu:15251 |
UCSCi | uc007nwo.2, mouse [Q61221-2] uc007nwq.2, mouse [Q61221-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U59496 Genomic DNA Translation: AAC52730.1 AF003695 mRNA Translation: AAC53455.1 AF004155 , AF004141, AF004142, AF004143, AF004144, AF004145, AF004146, AF004147, AF004148, AF004149, AF004150, AF004151, AF004152, AF004153, AF004154 Genomic DNA Translation: AAC53461.1 Y09085 Genomic DNA Translation: CAA70305.1 Y09085, Y13656 Genomic DNA Translation: CAA70306.1 AK034087 mRNA Translation: BAC28578.1 AK076395 mRNA Translation: BAC36320.1 AK033471 mRNA Translation: BAC28305.1 AK017853 mRNA Translation: BAB30975.1 BC026139 mRNA Translation: AAH26139.1 X95580 mRNA Translation: CAA64833.1 X95002 mRNA Translation: CAA64458.1 |
CCDSi | CCDS25977.1 [Q61221-1] |
PIRi | JC4837 |
RefSeqi | NP_001300848.1, NM_001313919.1 NP_001300849.1, NM_001313920.1 NP_034561.2, NM_010431.2 [Q61221-1] XP_017170450.1, XM_017314961.1 [Q61221-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4ZPR | X-ray | 3.90 | B | 13-357 | [»] | |
SMRi | Q61221 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 200304, 25 interactors |
DIPi | DIP-31083N |
IntActi | Q61221, 16 interactors |
MINTi | Q61221 |
STRINGi | 10090.ENSMUSP00000021530 |
Chemistry databases
BindingDBi | Q61221 |
ChEMBLi | CHEMBL6046 |
PTM databases
iPTMneti | Q61221 |
PhosphoSitePlusi | Q61221 |
Proteomic databases
PaxDbi | Q61221 |
PRIDEi | Q61221 |
ProteomicsDBi | 273106 [Q61221-1] 273107 [Q61221-2] |
Protocols and materials databases
Antibodypediai | 84, 2266 antibodies from 52 providers |
DNASUi | 15251 |
Genome annotation databases
Ensembli | ENSMUST00000021530; ENSMUSP00000021530; ENSMUSG00000021109 [Q61221-1] |
GeneIDi | 15251 |
KEGGi | mmu:15251 |
UCSCi | uc007nwo.2, mouse [Q61221-2] uc007nwq.2, mouse [Q61221-1] |
Organism-specific databases
CTDi | 3091 |
MGIi | MGI:106918, Hif1a |
VEuPathDBi | HostDB:ENSMUSG00000021109 |
Phylogenomic databases
eggNOGi | KOG3558, Eukaryota |
GeneTreei | ENSGT00940000156774 |
InParanoidi | Q61221 |
OMAi | TQMQEPA |
OrthoDBi | 547545at2759 |
PhylomeDBi | Q61221 |
TreeFami | TF317772 |
Enzyme and pathway databases
Reactomei | R-MMU-1234158, Regulation of gene expression by Hypoxia-inducible Factor R-MMU-1234174, Cellular response to hypoxia R-MMU-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-MMU-5689880, Ub-specific processing proteases R-MMU-8857538, PTK6 promotes HIF1A stabilization R-MMU-8951664, Neddylation |
Miscellaneous databases
BioGRID-ORCSi | 15251, 8 hits in 68 CRISPR screens |
ChiTaRSi | Hif1a, mouse |
PROi | PR:Q61221 |
RNActi | Q61221, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021109, Expressed in retina and 349 other tissues |
ExpressionAtlasi | Q61221, baseline and differential |
Genevisiblei | Q61221, MM |
Family and domain databases
CDDi | cd00130, PAS, 2 hits |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR001321, HIF-1_alpha IPR014887, HIF-1_TAD_C IPR021537, HIF_alpha_subunit IPR036638, HLH_DNA-bd_sf IPR001610, PAC IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR013655, PAS_fold_3 |
Pfami | View protein in Pfam PF11413, HIF-1, 1 hit PF08778, HIF-1a_CTAD, 1 hit PF00989, PAS, 1 hit PF08447, PAS_3, 1 hit |
PRINTSi | PR01080, HYPOXIAIF1A |
SMARTi | View protein in SMART SM00353, HLH, 1 hit SM00086, PAC, 1 hit SM00091, PAS, 2 hits |
SUPFAMi | SSF47459, SSF47459, 1 hit SSF55785, SSF55785, 2 hits |
TIGRFAMsi | TIGR00229, sensory_box, 2 hits |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS50112, PAS, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | HIF1A_MOUSE | |
Accessioni | Q61221Primary (citable) accession number: Q61221 Secondary accession number(s): O08741 Q9CYA8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 3, 2003 | |
Last modified: | February 23, 2022 | |
This is version 227 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references