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Protein

Double-strand break repair protein MRE11

Gene

Mre11

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.By similarity

Cofactori

Mn2+By similarity

Activity regulationi

Interaction with SAMHD1 stimulates the double-strand-specific 3'-5' exonuclease activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei129Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair, Meiosis
LigandManganese

Enzyme and pathway databases

ReactomeiR-MMU-1834949 Cytosolic sensors of pathogen-associated DNA
R-MMU-2559586 DNA Damage/Telomere Stress Induced Senescence
R-MMU-5685938 HDR through Single Strand Annealing (SSA)
R-MMU-5685939 HDR through MMEJ (alt-NHEJ)
R-MMU-5685942 HDR through Homologous Recombination (HRR)
R-MMU-5693548 Sensing of DNA Double Strand Breaks
R-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-MMU-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-MMU-5693571 Nonhomologous End-Joining (NHEJ)
R-MMU-5693579 Homologous DNA Pairing and Strand Exchange
R-MMU-5693607 Processing of DNA double-strand break ends
R-MMU-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-69473 G2/M DNA damage checkpoint

Names & Taxonomyi

Protein namesi
Recommended name:
Double-strand break repair protein MRE11 (EC:3.1.-.-By similarity)
Alternative name(s):
Double-strand break repair protein MRE11A
Short name:
MmMRE11A
Meiotic recombination 11 homolog 1
Short name:
MRE11 homolog 1
Meiotic recombination 11 homolog A
Short name:
MRE11 homolog A
Gene namesi
Name:Mre11
Synonyms:Mre11a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1100512 Mre11a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001386742 – 706Double-strand break repair protein MRE11Add BLAST705

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineCombined sources1
Cross-linki255Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei275PhosphoserineBy similarity1
Modified residuei618PhosphoserineBy similarity1
Modified residuei640PhosphoserineCombined sources1
Modified residuei648PhosphoserineCombined sources1
Modified residuei676PhosphoserineBy similarity1
Modified residuei686PhosphoserineCombined sources1
Modified residuei699PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61216
PaxDbiQ61216
PRIDEiQ61216

PTM databases

iPTMnetiQ61216
PhosphoSitePlusiQ61216

Miscellaneous databases

PMAP-CutDBiQ61216

Expressioni

Gene expression databases

BgeeiENSMUSG00000031928 Expressed in 262 organ(s), highest expression level in ear
ExpressionAtlasiQ61216 baseline and differential
GenevisibleiQ61216 MM

Interactioni

Subunit structurei

Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN. As part of the MRN complex, interacts with MCM9; the interaction recruits the complex to DNA repair sites. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN. Found in a complex with TERF2. Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo. Interacts with ATF2. Interacts with EXD2. Interacts with MRNIP. Interacts with SAMHD1; leading to stimulate 3'-5' exonuclease activity.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
NbnQ9R2072EBI-2014813,EBI-2014862

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201486, 5 interactors
DIPiDIP-46803N
IntActiQ61216, 4 interactors
STRINGi10090.ENSMUSP00000034405

Structurei

3D structure databases

ProteinModelPortaliQ61216
SMRiQ61216
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MRE11/RAD32 family.Curated

Phylogenomic databases

eggNOGiKOG2310 Eukaryota
COG0420 LUCA
GeneTreeiENSGT00390000017288
HOGENOMiHOG000216581
HOVERGENiHBG052508
InParanoidiQ61216
KOiK10865
OMAiRMFVNKQ
OrthoDBiEOG091G06P4
PhylomeDBiQ61216
TreeFamiTF101105

Family and domain databases

Gene3Di3.30.110.110, 1 hit
3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR003701 Mre11
IPR007281 Mre11_DNA-bd
IPR038487 Mre11_DNA-bd_sf
PANTHERiPTHR10139 PTHR10139, 1 hit
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF04152 Mre11_DNA_bind, 1 hit
PIRSFiPIRSF000882 DSB_repair_MRE11, 1 hit
SMARTiView protein in SMART
SM01347 Mre11_DNA_bind, 1 hit
TIGRFAMsiTIGR00583 mre11, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q61216-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSPTDPLDDE DTFKILVATD IHLGFMEKDA VRGNDTFVTF DEILRLALEN
60 70 80 90 100
EVDFILLGGD LFHENKPSRK TLHSCLELLR KYCMGDRPVQ FEVISDQSVN
110 120 130 140 150
FGFSKFPWVN YQDGNLNISI PVFSIHGNHD DPTGADALCA LDVLSCAGFV
160 170 180 190 200
NHFGRSMSVE KVDISPVLLQ KGSTKLALYG LGSIPDERLY RMFVNKKVTM
210 220 230 240 250
LRPKEDENSW FNLFVIHQNR SKHGNTNFIP EQFLDDFIDL VIWGHEHECK
260 270 280 290 300
IGPIKNEQQL FYVSQPGSSV VTSLSPGEAV KKHVGLLRIK GRKMNMQKLP
310 320 330 340 350
LRTVRRFFIE DVVLANHPNL FNPDNPKVTQ AIQSFCLEKI EEMLDSAERE
360 370 380 390 400
RLGNPQQPGK PLIRLRVDYS GGFEPFNVLR FSQKFVDRVA NPKDVIHFFR
410 420 430 440 450
HREQKGKTGE EINFGMLITK PASEGATLRV EDLVKQYFQT AEKNVQLSLL
460 470 480 490 500
TERGMGEAVQ EFVDKEEKDA IEELVKYQLE KTQRFLKERH IDALEDKIDE
510 520 530 540 550
EVRRFRESRQ RNTNEEDDEV REAMSRARAL RSQSETSTSA FSAEDLSFDT
560 570 580 590 600
SEQTANDSDD SLSAVPSRGR GRGRGRRGAR GQSSAPRGGS QRGRDTGLEI
610 620 630 640 650
TTRGRSSKAT SSTSRNMSII DAFRSTRQQP SRNVAPKNYS ETIEVDDSDE
660 670 680 690 700
DDIFPTNSRA DQRWSGTTSS KRMSQSQTAK GVDFESDEDD DDDPFMSSSC

PRRNRR
Length:706
Mass (Da):80,223
Last modified:November 1, 1996 - v1
Checksum:i0F12F51902FC179A
GO
Isoform 2 (identifier: Q61216-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     340-366: Missing.

Show »
Length:679
Mass (Da):77,094
Checksum:i3ACDE474E0425430
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6RX99F6RX99_MOUSE
MRE11A homolog A, double strand bre...
Mre11a
170Annotation score:
B2KF76B2KF76_MOUSE
MRE11A homolog A, double strand bre...
Mre11a
203Annotation score:
A0A1L1SQT7A0A1L1SQT7_MOUSE
MRE11A homolog A, double strand bre...
Mre11a
57Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003263340 – 366Missing in isoform 2. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58987 mRNA Translation: AAB04955.1
U60318 mRNA Translation: AAB03664.1
BC065144 mRNA Translation: AAH65144.1
CCDSiCCDS22827.1 [Q61216-1]
CCDS80958.1 [Q61216-2]
RefSeqiNP_001297657.1, NM_001310728.1 [Q61216-2]
NP_061206.1, NM_018736.3 [Q61216-1]
XP_006510111.1, XM_006510048.2 [Q61216-1]
XP_006510112.1, XM_006510049.2 [Q61216-2]
UniGeneiMm.149071

Genome annotation databases

EnsembliENSMUST00000034405; ENSMUSP00000034405; ENSMUSG00000031928 [Q61216-1]
ENSMUST00000115632; ENSMUSP00000111295; ENSMUSG00000031928 [Q61216-2]
GeneIDi17535
KEGGimmu:17535
UCSCiuc009ofc.1 mouse [Q61216-1]
uc009ofd.1 mouse [Q61216-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58987 mRNA Translation: AAB04955.1
U60318 mRNA Translation: AAB03664.1
BC065144 mRNA Translation: AAH65144.1
CCDSiCCDS22827.1 [Q61216-1]
CCDS80958.1 [Q61216-2]
RefSeqiNP_001297657.1, NM_001310728.1 [Q61216-2]
NP_061206.1, NM_018736.3 [Q61216-1]
XP_006510111.1, XM_006510048.2 [Q61216-1]
XP_006510112.1, XM_006510049.2 [Q61216-2]
UniGeneiMm.149071

3D structure databases

ProteinModelPortaliQ61216
SMRiQ61216
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201486, 5 interactors
DIPiDIP-46803N
IntActiQ61216, 4 interactors
STRINGi10090.ENSMUSP00000034405

PTM databases

iPTMnetiQ61216
PhosphoSitePlusiQ61216

Proteomic databases

EPDiQ61216
PaxDbiQ61216
PRIDEiQ61216

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034405; ENSMUSP00000034405; ENSMUSG00000031928 [Q61216-1]
ENSMUST00000115632; ENSMUSP00000111295; ENSMUSG00000031928 [Q61216-2]
GeneIDi17535
KEGGimmu:17535
UCSCiuc009ofc.1 mouse [Q61216-1]
uc009ofd.1 mouse [Q61216-2]

Organism-specific databases

CTDi17535
MGIiMGI:1100512 Mre11a

Phylogenomic databases

eggNOGiKOG2310 Eukaryota
COG0420 LUCA
GeneTreeiENSGT00390000017288
HOGENOMiHOG000216581
HOVERGENiHBG052508
InParanoidiQ61216
KOiK10865
OMAiRMFVNKQ
OrthoDBiEOG091G06P4
PhylomeDBiQ61216
TreeFamiTF101105

Enzyme and pathway databases

ReactomeiR-MMU-1834949 Cytosolic sensors of pathogen-associated DNA
R-MMU-2559586 DNA Damage/Telomere Stress Induced Senescence
R-MMU-5685938 HDR through Single Strand Annealing (SSA)
R-MMU-5685939 HDR through MMEJ (alt-NHEJ)
R-MMU-5685942 HDR through Homologous Recombination (HRR)
R-MMU-5693548 Sensing of DNA Double Strand Breaks
R-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-MMU-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-MMU-5693571 Nonhomologous End-Joining (NHEJ)
R-MMU-5693579 Homologous DNA Pairing and Strand Exchange
R-MMU-5693607 Processing of DNA double-strand break ends
R-MMU-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-69473 G2/M DNA damage checkpoint

Miscellaneous databases

PMAP-CutDBiQ61216
PROiPR:Q61216
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031928 Expressed in 262 organ(s), highest expression level in ear
ExpressionAtlasiQ61216 baseline and differential
GenevisibleiQ61216 MM

Family and domain databases

Gene3Di3.30.110.110, 1 hit
3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR003701 Mre11
IPR007281 Mre11_DNA-bd
IPR038487 Mre11_DNA-bd_sf
PANTHERiPTHR10139 PTHR10139, 1 hit
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF04152 Mre11_DNA_bind, 1 hit
PIRSFiPIRSF000882 DSB_repair_MRE11, 1 hit
SMARTiView protein in SMART
SM01347 Mre11_DNA_bind, 1 hit
TIGRFAMsiTIGR00583 mre11, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMRE11_MOUSE
AccessioniPrimary (citable) accession number: Q61216
Secondary accession number(s): Q62430
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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