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Protein

Methylosome subunit pICln

Gene

Clns1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in both the assembly of spliceosomal snRNPs and the methylation of Sm proteins (By similarity). Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also indirectly participate in cellular volume control by activation of a swelling-induced chloride conductance pathway.By similarity

GO - Molecular functioni

GO - Biological processi

  • cell volume homeostasis Source: MGI
  • chloride transport Source: MGI
  • negative regulation of cell volume Source: CAFA
  • spliceosomal snRNP assembly Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
Methylosome subunit pICln
Alternative name(s):
Chloride channel, nucleotide sensitive 1A
Chloride conductance regulatory protein ICln
Short name:
I(Cln)
Chloride ion current inducer protein
Short name:
ClCI
Gene namesi
Name:Clns1a
Synonyms:Clci, Clcni
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:109638 Clns1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001851562 – 236Methylosome subunit pIClnAdd BLAST235

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei95PhosphoserineBy similarity1
Modified residuei143PhosphoserineBy similarity1
Modified residuei192PhosphoserineBy similarity1
Modified residuei194PhosphoserineBy similarity1
Modified residuei197PhosphoserineBy similarity1
Modified residuei209PhosphoserineBy similarity1
Modified residuei222PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ61189
MaxQBiQ61189
PaxDbiQ61189
PeptideAtlasiQ61189
PRIDEiQ61189

PTM databases

iPTMnetiQ61189

Expressioni

Gene expression databases

CleanExiMM_CLNS1A

Interactioni

Subunit structurei

Component of the methylosome, a 20S complex containing at least PRMT5/SKB1, WDR77/MEP50 and CLNS1A/pICln. May mediate SNRPD1 and SNRPD3 methylation. Forms a 6S pICln-Sm complex composed of CLNS1A/pICln, SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG; ring-like structure where CLNS1A/pICln mimics additional Sm proteins and which is unable to assemble into the core snRNP. Interacts with LSM10 and LSM11.By similarity

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-1023 Methylosome
STRINGi10090.ENSMUSP00000026506

Structurei

3D structure databases

ProteinModelPortaliQ61189
SMRiQ61189
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3238 Eukaryota
ENOG4111R94 LUCA
HOVERGENiHBG003108
InParanoidiQ61189

Family and domain databases

InterProiView protein in InterPro
IPR003521 ICln
IPR039924 ICln/Lot5
PANTHERiPTHR21399 PTHR21399, 1 hit
PfamiView protein in Pfam
PF03517 Voldacs, 1 hit
PRINTSiPR01348 ICLNCHANNEL

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q61189-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSFLKSFPPP GSADGLRLQQ PDTEAVLNGK GLGTGTLYIA ESRLSWLDGS
60 70 80 90 100
GLGFSLEYPT ISLHAVSRDP NAYPQEHLYV MVNAKLGEES KEPPSDEDEE
110 120 130 140 150
DNDDIEPISE FRFVPSDKSA LEAMFTAMCE CQALHPDPED EDSDDYDGEE
160 170 180 190 200
YDVEAHEQGQ GDIPTFYTYE EGLSHLTAEG QATLERLEGM LSQSVSSQYN
210 220 230
MAGVRTEDSV RNYEDGMEVE TTPTVAGQFE DADVDH
Length:236
Mass (Da):26,021
Last modified:November 1, 1996 - v1
Checksum:iC3CEB17F4B94950E
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q923F1Q923F1_MOUSE
Chloride channel, nucleotide-sensit...
Clns1a mCG_7058
241Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53455 mRNA Translation: AAB03317.1
UniGeneiMm.21482

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53455 mRNA Translation: AAB03317.1
UniGeneiMm.21482

3D structure databases

ProteinModelPortaliQ61189
SMRiQ61189
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-1023 Methylosome
STRINGi10090.ENSMUSP00000026506

PTM databases

iPTMnetiQ61189

Proteomic databases

EPDiQ61189
MaxQBiQ61189
PaxDbiQ61189
PeptideAtlasiQ61189
PRIDEiQ61189

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:109638 Clns1a

Phylogenomic databases

eggNOGiKOG3238 Eukaryota
ENOG4111R94 LUCA
HOVERGENiHBG003108
InParanoidiQ61189

Miscellaneous databases

PROiPR:Q61189
SOURCEiSearch...

Gene expression databases

CleanExiMM_CLNS1A

Family and domain databases

InterProiView protein in InterPro
IPR003521 ICln
IPR039924 ICln/Lot5
PANTHERiPTHR21399 PTHR21399, 1 hit
PfamiView protein in Pfam
PF03517 Voldacs, 1 hit
PRINTSiPR01348 ICLNCHANNEL
ProtoNetiSearch...

Entry informationi

Entry nameiICLN_MOUSE
AccessioniPrimary (citable) accession number: Q61189
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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