Scnn1a

Functionⁱ

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception.1 Publication

Enzyme regulationⁱ

Activated by WNK1, WNK2, WNK3 and WNK4.1 Publication

GO - Molecular functionⁱ

actin binding Source: MGI
ligand-gated sodium channel activity
Source: MGI
Inferred from direct assayⁱ
- 10409305
WW domain binding
Source: MGI
Inferred from physical interactionⁱ
- 10212229

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

multicellular organismal water homeostasis Source: UniProtKB
regulation of body fluid levels Source: MGI
response to stimulus Source: UniProtKB-KW
sensory perception of taste Source: UniProtKB-KW
sodium ion homeostasis Source: UniProtKB
sodium ion transmembrane transport Source: MGI
sodium ion transport
Source: MGI
Inferred from direct assayⁱ
- 10409305

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Ion channel, Sodium channel
Biological process	Ion transport, Sensory transduction, Sodium transport, Taste, Transport
Ligand	Sodium

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Amiloride-sensitive sodium channel subunit alpha Alternative name(s): Alpha-NaCH Epithelial Na(+) channel subunit alpha Short name: Alpha-ENaC Nonvoltage-gated sodium channel 1 subunit alpha SCNEA
Gene namesⁱ	Name:Scnn1a
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Unplaced

Organism-specific databases

MGIⁱ	MGI:101782 Scnn1a

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 110	CytoplasmicBy similarityAdd BLAST	110
Transmembraneⁱ	111 – 131	Helical; Name=1Sequence analysisAdd BLAST	21
Topological domainⁱ	132 – 589	ExtracellularBy similarityAdd BLAST	458
Transmembraneⁱ	590 – 610	Helical; Name=2Sequence analysisAdd BLAST	21
Topological domainⁱ	611 – 699	CytoplasmicBy similarityAdd BLAST	89

Keywords - Cellular componentⁱ

Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Flagellum, Membrane

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL3608200

ChEMBLⁱ

CHEMBL3608200

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000181262	1 – 699	Amiloride-sensitive sodium channel subunit alphaAdd BLAST		699

Post-translational modificationⁱ

Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.By similarity

ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation.By similarity

N-glycosylated.By similarity

Keywords - PTMⁱ

Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbⁱ	Q61180
PRIDEⁱ	Q61180

PTM databases

iPTMnetⁱ	Q61180
PhosphoSitePlusⁱ	Q61180

Miscellaneous databases

PMAP-CutDBⁱ	Q61180

PMAP-CutDBⁱ

Q61180

Expressionⁱ

Tissue specificityⁱ

Expressed in kidney and lung (at protein level). Also expressed in distal colon and, at low levels, in liver.1 Publication

Gene expression databases

CleanExⁱ	MM_SCNN1A

CleanExⁱ

MM_SCNN1A

Interactionⁱ

Subunit structureⁱ

Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in some organisms and can replace the alpha/SCNN1A subunit to form an alternative channel with specific properties (Probable). Interacts with NEDD4 (via WW domains) (PubMed:11244092, PubMed:15123669). Interacts with NEDD4L (via WW domains) (PubMed:11244092, PubMed:12424229, PubMed:15123669). Interacts with WWP1 (via WW domains). Interacts with WWP2 (via WW domains). Interacts with the full-length immature form of PCSK9 (pro-PCSK9).By similarityCurated3 Publications

GO - Molecular functionⁱ

actin binding Source: MGI
WW domain binding
Source: MGI
Inferred from physical interactionⁱ
- 10212229

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	203105, 4 interactors
ComplexPortalⁱ	CPX-315 Amiloride-sensitive sodium channel complex, alpha-beta-gamma
Database of interacting proteins More...DIPⁱ	DIP-40889N
IntActⁱ	Q61180, 1 interactor
STRINGⁱ	10090.ENSMUSP00000080164

Chemistry databases

BindingDBⁱ

Q61180

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q61180
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1A subfamily. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG4294 Eukaryota ENOG410ZNFK LUCA
HOVERGENⁱ	HBG058435
InParanoidⁱ	Q61180
KEGG Orthology (KO) More...KOⁱ	K04824
OMAⁱ	VEYCDYR
PhylomeDBⁱ	Q61180

Family and domain databases

InterProⁱ	View protein in InterPro IPR001873 ENaC IPR004724 ENaC_chordates IPR020903 ENaC_CS
PANTHERⁱ	PTHR11690 PTHR11690, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00858 ASC, 1 hit
PRINTSⁱ	PR01078 AMINACHANNEL
TIGRFAMsⁱ	TIGR00859 ENaC, 1 hit
PROSITEⁱ	View protein in PROSITE PS01206 ASC, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q61180-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLDHTRAPEL NLDLDLDVSN SPKGSMKGNN FKEQDLCPPL PMQGLGKGDK 
        60         70         80         90        100
REEQALGPEP SEPRQPTEEE EALIEFHRSY RELFQFFCNN TTIHGAIRLV 
       110        120        130        140        150
CSKHNRMKTA FWAVLWLCTF GMMYWQFALL FEEYFSYPVS LNINLNSDKL 
       160        170        180        190        200
VFPAVTVCTL NPYRYTEIKE DLEELDRITE QTLFDLYKYN SSYTRQAGGR 
       210        220        230        240        250
RRSTRDLRGA LPHPLQRLRT PPPPNPARSA RSASSSVRDN NPQVDRKDWK 
       260        270        280        290        300
IGFQLCNQNK SDCFYQTYSS GVDAVREWYR FHYINILSRL PDTSPALEEE 
       310        320        330        340        350
ALGSFIFTCR FNQAPCNQAN YSQFHHPMYG NCYTFNNKNN SNLWMSSMPG 
       360        370        380        390        400
VNNGLSLTLR TEQNDFIPLL STVTGARVMV HGQDEPAFMD DGGFNVRPGV 
       410        420        430        440        450
ETSISMRKEA LDSLGGNYGD CTENGSDVPV KNLYPSKYTQ QVCIHSCFQE 
       460        470        480        490        500
NMIKKCGCAY IFYPKPKGVE FCDYLKQSSW GYCYYKLQAA FSLDSLGCFS 
       510        520        530        540        550
KCRKPCSVTN YKLSAGYSRW PSVKSQDWIF EMLSLQNNYT INNKRNGVAK 
       560        570        580        590        600
LNIFFKELNY KTNSESPSVT MVSLLSNLGS QWSLWFGSSV LSVVEMAELI 
       610        620        630        640        650
FDLLVITLIM LLHRFRSRYW SPGRGARGAR EVASTPASSF PSRFCPHPTS 
       660        670        680        690 
PPPSLPQQGT TPPLALTAPP PAYATLGPSA SPLDSAVPGS SACAPAMAL

Length:699

Mass (Da):78,893

Last modified:December 1, 2000 - v2

Checksum:ⁱ5B083BE8769B017A

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	445	H → R in AAA97412 (PubMed:9284273).Curated		1
Sequence conflictⁱ	555 – 557	FKE → YKH in AAA97412 (PubMed:9284273).Curated		3

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF112185 mRNA Translation: AAD21244.1 U52006 mRNA Translation: AAA97412.1
NCBI Reference Sequences More...RefSeqⁱ	NP_035454.2, NM_011324.2
UniGeneⁱ	Mm.144114

Genome annotation databases

GeneIDⁱ	20276
KEGGⁱ	mmu:20276

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
Q61180	Amiloride-sensitive sodium channel subunit alpha		MOUSE		700
Amiloride-sensitive sodium channel subunit alpha		MOUSE		674
Amiloride-sensitive sodium channel subunit alpha		MOUSE		593
Scnn1a protein (Fragment)		MOUSE		410
Epithelial sodium channel subunit alpha (Fragment)		MOUSE		59
+2

Protein	Similar proteins		Species	Score	Length
Q61180	Amiloride-sensitive sodium channel subunit alpha		RAT		698
UPI000A326E8D		MESAU		729
UPI00077DDEE7		PERMB		712
UPI000454A5CE		CRIGR		704
Amiloride-sensitive sodium channel subunit alpha		MOUSE		700
+14

Protein	Similar proteins		Species	Score	Length
Q61180	Amiloride-sensitive sodium channel subunit alpha		RAT		698
UPI000A326E8D		MESAU		729
UPI00077DDEE7		PERMB		712
UPI000454A5CE		CRIGR		704
Amiloride-sensitive sodium channel subunit alpha		MOUSE		700
+37

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF112185 mRNA Translation: AAD21244.1 U52006 mRNA Translation: AAA97412.1
NCBI Reference Sequences More...RefSeqⁱ	NP_035454.2, NM_011324.2
UniGeneⁱ	Mm.144114

3D structure databases

ProteinModelPortalⁱ	Q61180
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	203105, 4 interactors
ComplexPortalⁱ	CPX-315 Amiloride-sensitive sodium channel complex, alpha-beta-gamma
Database of interacting proteins More...DIPⁱ	DIP-40889N
IntActⁱ	Q61180, 1 interactor
STRINGⁱ	10090.ENSMUSP00000080164

Chemistry databases

BindingDBⁱ	Q61180
ChEMBLⁱ	CHEMBL3608200

PTM databases

iPTMnetⁱ	Q61180
PhosphoSitePlusⁱ	Q61180

Proteomic databases

PaxDbⁱ	Q61180
PRIDEⁱ	Q61180

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	20276
KEGGⁱ	mmu:20276

Organism-specific databases

CTDⁱ	6337
MGIⁱ	MGI:101782 Scnn1a

Phylogenomic databases

eggNOGⁱ	KOG4294 Eukaryota ENOG410ZNFK LUCA
HOVERGENⁱ	HBG058435
InParanoidⁱ	Q61180
KEGG Orthology (KO) More...KOⁱ	K04824
OMAⁱ	VEYCDYR
PhylomeDBⁱ	Q61180

Miscellaneous databases

ChiTaRSⁱ	Scnn1a mouse
PMAP-CutDBⁱ	Q61180
Protein Ontology More...PROⁱ	PR:Q61180
SOURCEⁱ	Search...

Gene expression databases

CleanExⁱ	MM_SCNN1A

CleanExⁱ

MM_SCNN1A

Family and domain databases

InterProⁱ	View protein in InterPro IPR001873 ENaC IPR004724 ENaC_chordates IPR020903 ENaC_CS
PANTHERⁱ	PTHR11690 PTHR11690, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00858 ASC, 1 hit
PRINTSⁱ	PR01078 AMINACHANNEL
TIGRFAMsⁱ	TIGR00859 ENaC, 1 hit
PROSITEⁱ	View protein in PROSITE PS01206 ASC, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SCNNA_MOUSE
Accessionⁱ	Q61180Primary (citable) accession number: Q61180 Secondary accession number(s): Q9WU37
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	December 1, 2000
	Last modified:	June 20, 2018
	This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q61180 (SCNNA_MOUSE)

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Amiloride-sensitive sodium channel subunit alpha

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes an enzyme regulatory mechanism and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/enzyme_regulation' target='_top'>More...</a></p>Enzyme regulationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Other locations

Cytoskeleton

Cytosol

Extracellular region or secreted

Plasma Membrane

Other locations

Topology

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

Miscellaneous databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Enzyme regulationⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ