UniProtKB - Q61180 (SCNNA_MOUSE)
Amiloride-sensitive sodium channel subunit alpha
Scnn1a
Functioni
Activity regulationi
GO - Molecular functioni
- actin binding Source: MGI
- ligand-gated sodium channel activity Source: MGI
- WW domain binding Source: MGI
GO - Biological processi
- cellular response to aldosterone Source: MGI
- multicellular organismal water homeostasis Source: UniProtKB
- regulation of body fluid levels Source: MGI
- sensory perception of taste Source: UniProtKB-KW
- sodium ion homeostasis Source: UniProtKB
- sodium ion transmembrane transport Source: MGI
- sodium ion transport Source: MGI
Keywordsi
Molecular function | Ion channel, Sodium channel |
Biological process | Ion transport, Sensory transduction, Sodium transport, Taste, Transport |
Ligand | Sodium |
Enzyme and pathway databases
Reactomei | R-MMU-2672351, Stimuli-sensing channels |
Names & Taxonomyi
Protein namesi | Recommended name: Amiloride-sensitive sodium channel subunit alphaAlternative name(s): Alpha-NaCH Epithelial Na(+) channel subunit alpha Short name: Alpha-ENaC Nonvoltage-gated sodium channel 1 subunit alpha SCNEA |
Gene namesi | Name:Scnn1a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:101782, Scnn1a |
Subcellular locationi
Plasma membrane
- Apical cell membrane By similarity; Multi-pass membrane protein By similarity
Other locations
- cilium By similarity
- Cytoplasmic granule By similarity
- Cytoplasm By similarity
- acrosome By similarity
- flagellum By similarity
Note: In the oviduct and bronchus, located on cilia in multi-ciliated cells. In endometrial non-ciliated epithelial cells, restricted to apical surfaces. In epidermis, located nearly uniformly in the cytoplasm in a granular distribution. In sebaceous glands, observed only in the cytoplasmic space in between the lipid vesicles. In eccrine sweat glands, mainly located at the apical surface of the cells facing the lumen. In skin, in arrector pili muscle cells and in adipocytes, located in the cytoplasm and colocalized with actin fibers. In spermatogonia, spermatocytes and round spermatids, located in the cytoplasm. Prior to spermiation, location shifts from the cytoplasm to the spermatid tail. In spermatozoa, localizes at the acrosome and the central region of the sperm flagellum.By similarity
Cytoskeleton
- cortical actin cytoskeleton Source: MGI
Cytosol
- cytosol Source: MGI
Extracellular region or secreted
- extracellular exosome Source: MGI
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- ciliary membrane Source: UniProtKB
- external side of plasma membrane Source: MGI
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: MGI
- plasma membrane protein complex Source: MGI
Other locations
- acrosomal vesicle Source: UniProtKB
- cytoplasm Source: UniProtKB
- integral component of membrane Source: MGI
- membrane Source: MGI
- motile cilium Source: UniProtKB
- sodium channel complex Source: MGI
- sperm principal piece Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 110 | CytoplasmicBy similarityAdd BLAST | 110 | |
Transmembranei | 111 – 131 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 132 – 589 | ExtracellularBy similarityAdd BLAST | 458 | |
Transmembranei | 590 – 610 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 611 – 699 | CytoplasmicBy similarityAdd BLAST | 89 |
Keywords - Cellular componenti
Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Flagellum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000181262 | 1 – 699 | Amiloride-sensitive sodium channel subunit alphaAdd BLAST | 699 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, Ubl conjugationProteomic databases
PaxDbi | Q61180 |
PRIDEi | Q61180 |
PTM databases
iPTMneti | Q61180 |
PhosphoSitePlusi | Q61180 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in some organisms and can replace the alpha/SCNN1A subunit to form an alternative channel with specific properties (Probable).
Interacts with NEDD4 (via WW domains) (PubMed:11244092, PubMed:15123669).
Interacts with NEDD4L (via WW domains) (PubMed:11244092, PubMed:12424229, PubMed:15123669).
Interacts with WWP1 (via WW domains).
Interacts with WWP2 (via WW domains).
Interacts with the full-length immature form of PCSK9 (pro-PCSK9).
By similarityCurated3 PublicationsGO - Molecular functioni
- actin binding Source: MGI
- WW domain binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 203105, 7 interactors |
ComplexPortali | CPX-315, Amiloride-sensitive sodium channel complex, alpha-beta-gamma |
DIPi | DIP-40889N |
IntActi | Q61180, 1 interactor |
STRINGi | 10090.ENSMUSP00000080164 |
Chemistry databases
BindingDBi | Q61180 |
Miscellaneous databases
RNActi | Q61180, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4294, Eukaryota |
InParanoidi | Q61180 |
OrthoDBi | 686369at2759 |
PhylomeDBi | Q61180 |
Family and domain databases
InterProi | View protein in InterPro IPR001873, ENaC IPR004724, ENaC_chordates IPR020903, ENaC_CS |
PANTHERi | PTHR11690, PTHR11690, 1 hit |
Pfami | View protein in Pfam PF00858, ASC, 1 hit |
PRINTSi | PR01078, AMINACHANNEL |
TIGRFAMsi | TIGR00859, ENaC, 1 hit |
PROSITEi | View protein in PROSITE PS01206, ASC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MLDHTRAPEL NLDLDLDVSN SPKGSMKGNN FKEQDLCPPL PMQGLGKGDK
60 70 80 90 100
REEQALGPEP SEPRQPTEEE EALIEFHRSY RELFQFFCNN TTIHGAIRLV
110 120 130 140 150
CSKHNRMKTA FWAVLWLCTF GMMYWQFALL FEEYFSYPVS LNINLNSDKL
160 170 180 190 200
VFPAVTVCTL NPYRYTEIKE DLEELDRITE QTLFDLYKYN SSYTRQAGGR
210 220 230 240 250
RRSTRDLRGA LPHPLQRLRT PPPPNPARSA RSASSSVRDN NPQVDRKDWK
260 270 280 290 300
IGFQLCNQNK SDCFYQTYSS GVDAVREWYR FHYINILSRL PDTSPALEEE
310 320 330 340 350
ALGSFIFTCR FNQAPCNQAN YSQFHHPMYG NCYTFNNKNN SNLWMSSMPG
360 370 380 390 400
VNNGLSLTLR TEQNDFIPLL STVTGARVMV HGQDEPAFMD DGGFNVRPGV
410 420 430 440 450
ETSISMRKEA LDSLGGNYGD CTENGSDVPV KNLYPSKYTQ QVCIHSCFQE
460 470 480 490 500
NMIKKCGCAY IFYPKPKGVE FCDYLKQSSW GYCYYKLQAA FSLDSLGCFS
510 520 530 540 550
KCRKPCSVTN YKLSAGYSRW PSVKSQDWIF EMLSLQNNYT INNKRNGVAK
560 570 580 590 600
LNIFFKELNY KTNSESPSVT MVSLLSNLGS QWSLWFGSSV LSVVEMAELI
610 620 630 640 650
FDLLVITLIM LLHRFRSRYW SPGRGARGAR EVASTPASSF PSRFCPHPTS
660 670 680 690
PPPSLPQQGT TPPLALTAPP PAYATLGPSA SPLDSAVPGS SACAPAMAL
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3USG4 | Q3USG4_MOUSE | Amiloride-sensitive sodium channel ... | Scnn1a | 700 | Annotation score: | ||
H3BJC3 | H3BJC3_MOUSE | Amiloride-sensitive sodium channel ... | Scnn1a | 674 | Annotation score: | ||
H3BKW1 | H3BKW1_MOUSE | Amiloride-sensitive sodium channel ... | Scnn1a | 275 | Annotation score: | ||
H3BKC4 | H3BKC4_MOUSE | Amiloride-sensitive sodium channel ... | Scnn1a | 591 | Annotation score: | ||
H3BLI2 | H3BLI2_MOUSE | Amiloride-sensitive sodium channel ... | Scnn1a | 593 | Annotation score: | ||
H3BKP2 | H3BKP2_MOUSE | Amiloride-sensitive sodium channel ... | Scnn1a | 143 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 445 | H → R in AAA97412 (PubMed:9284273).Curated | 1 | |
Sequence conflicti | 555 – 557 | FKE → YKH in AAA97412 (PubMed:9284273).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF112185 mRNA Translation: AAD21244.1 U52006 mRNA Translation: AAA97412.1 |
RefSeqi | NP_035454.2, NM_011324.2 |
Genome annotation databases
GeneIDi | 20276 |
KEGGi | mmu:20276 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF112185 mRNA Translation: AAD21244.1 U52006 mRNA Translation: AAA97412.1 |
RefSeqi | NP_035454.2, NM_011324.2 |
3D structure databases
SMRi | Q61180 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203105, 7 interactors |
ComplexPortali | CPX-315, Amiloride-sensitive sodium channel complex, alpha-beta-gamma |
DIPi | DIP-40889N |
IntActi | Q61180, 1 interactor |
STRINGi | 10090.ENSMUSP00000080164 |
Chemistry databases
BindingDBi | Q61180 |
ChEMBLi | CHEMBL3608200 |
PTM databases
iPTMneti | Q61180 |
PhosphoSitePlusi | Q61180 |
Proteomic databases
PaxDbi | Q61180 |
PRIDEi | Q61180 |
Genome annotation databases
GeneIDi | 20276 |
KEGGi | mmu:20276 |
Organism-specific databases
CTDi | 6337 |
MGIi | MGI:101782, Scnn1a |
Phylogenomic databases
eggNOGi | KOG4294, Eukaryota |
InParanoidi | Q61180 |
OrthoDBi | 686369at2759 |
PhylomeDBi | Q61180 |
Enzyme and pathway databases
Reactomei | R-MMU-2672351, Stimuli-sensing channels |
Miscellaneous databases
BioGRID-ORCSi | 20276, 3 hits in 17 CRISPR screens |
ChiTaRSi | Scnn1a, mouse |
PROi | PR:Q61180 |
RNActi | Q61180, protein |
SOURCEi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR001873, ENaC IPR004724, ENaC_chordates IPR020903, ENaC_CS |
PANTHERi | PTHR11690, PTHR11690, 1 hit |
Pfami | View protein in Pfam PF00858, ASC, 1 hit |
PRINTSi | PR01078, AMINACHANNEL |
TIGRFAMsi | TIGR00859, ENaC, 1 hit |
PROSITEi | View protein in PROSITE PS01206, ASC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SCNNA_MOUSE | |
Accessioni | Q61180Primary (citable) accession number: Q61180 Secondary accession number(s): Q9WU37 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | December 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 160 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families