UniProtKB - Q61171 (PRDX2_MOUSE)
Protein
Peroxiredoxin-2
Gene
Prdx2
Organism
Mus musculus (Mouse)
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2.By similarity
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.By similarity
Catalytic activityi
- EC:1.11.1.24By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 51 | Cysteine sulfenic acid (-SOH) intermediateBy similarity | 1 |
GO - Molecular functioni
- antioxidant activity Source: MGI
- peroxidase activity Source: MGI
- selenium binding Source: MGI
- thioredoxin peroxidase activity Source: MGI
GO - Biological processi
- activation of MAPK activity Source: MGI
- cell redox homeostasis Source: GO_Central
- cellular response to oxidative stress Source: MGI
- homeostasis of number of cells Source: MGI
- hydrogen peroxide catabolic process Source: MGI
- hydrogen peroxide metabolic process Source: MGI
- leukocyte activation Source: GO_Central
- negative regulation of extrinsic apoptotic signaling pathway Source: MGI
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- negative regulation of lipopolysaccharide-mediated signaling pathway Source: MGI
- negative regulation of neuron apoptotic process Source: MGI
- negative regulation of NF-kappaB transcription factor activity Source: MGI
- negative regulation of T cell differentiation Source: MGI
- positive regulation of blood coagulation Source: BHF-UCL
- regulation of apoptotic process Source: MGI
- regulation of hydrogen peroxide metabolic process Source: MGI
- removal of superoxide radicals Source: BHF-UCL
- respiratory burst involved in inflammatory response Source: MGI
- response to lipopolysaccharide Source: MGI
- response to oxidative stress Source: MGI
- T cell proliferation Source: MGI
- thymus development Source: MGI
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Enzyme and pathway databases
Reactomei | R-MMU-3299685, Detoxification of Reactive Oxygen Species R-MMU-5628897, TP53 Regulates Metabolic Genes |
Protein family/group databases
PeroxiBasei | 4474, Mm2CysPrx02 |
Names & Taxonomyi
Protein namesi | Recommended name: Peroxiredoxin-2 (EC:1.11.1.24By similarity)Alternative name(s): Thiol-specific antioxidant protein Short name: TSA Thioredoxin peroxidase 1 Thioredoxin-dependent peroxide reductase 1 Thioredoxin-dependent peroxiredoxin 2Curated |
Gene namesi | Name:Prdx2 Synonyms:Tdpx1, Tpx |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109486, Prdx2 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Other locations
- myelin sheath Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000135081 | 2 – 198 | Peroxiredoxin-2Add BLAST | 197 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanine1 Publication | 1 | |
Modified residuei | 11 | PhosphoserineCombined sources | 1 | |
Disulfide bondi | 51 | Interchain (with C-172); in linked formBy similarity | ||
Modified residuei | 112 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 172 | Interchain (with C-51); in linked formBy similarity | ||
Modified residuei | 182 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.By similarity
Keywords - PTMi
Acetylation, Disulfide bond, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3401 |
EPDi | Q61171 |
jPOSTi | Q61171 |
MaxQBi | Q61171 |
PaxDbi | Q61171 |
PeptideAtlasi | Q61171 |
PRIDEi | Q61171 |
TopDownProteomicsi | Q61171 |
2D gel databases
REPRODUCTION-2DPAGEi | Q61171 |
SWISS-2DPAGEi | Q61171 |
UCD-2DPAGEi | Q61171 |
PTM databases
iPTMneti | Q61171 |
PhosphoSitePlusi | Q61171 |
SwissPalmi | Q61171 |
Expressioni
Tissue specificityi
Widely expressed with highest levels in bone marrow. High levels also found in heart, brain, kidney and skeletal muscle. Lower levels in liver, lung and thymus.
Gene expression databases
Bgeei | ENSMUSG00000005161, Expressed in cortical plate and 299 other tissues |
ExpressionAtlasi | Q61171, baseline and differential |
Genevisiblei | Q61171, MM |
Interactioni
Subunit structurei
Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer (By similarity).
Interacts with TIPIN (PubMed:17141802).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 204094, 25 interactors |
IntActi | Q61171, 17 interactors |
MINTi | Q61171 |
STRINGi | 10090.ENSMUSP00000005292 |
Miscellaneous databases
RNActi | Q61171, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 164 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 159 |
Sequence similaritiesi
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | KOG0852, Eukaryota |
GeneTreei | ENSGT00940000155828 |
HOGENOMi | CLU_042529_21_0_1 |
InParanoidi | Q61171 |
OMAi | FAFTGVC |
OrthoDBi | 1326484at2759 |
PhylomeDBi | Q61171 |
TreeFami | TF105181 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q61171-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASGNAQIGK SAPDFTATAV VDGAFKEIKL SDYRGKYVVL FFYPLDFTFV
60 70 80 90 100
CPTEIIAFSD HAEDFRKLGC EVLGVSVDSQ FTHLAWINTP RKEGGLGPLN
110 120 130 140 150
IPLLADVTKS LSQNYGVLKN DEGIAYRGLF IIDAKGVLRQ ITVNDLPVGR
160 170 180 190
SVDEALRLVQ AFQYTDEHGE VCPAGWKPGS DTIKPNVDDS KEYFSKHN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3Z4A4 | D3Z4A4_MOUSE | Peroxiredoxin-2 | Prdx2 | 146 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 38 | V → M in BAB27093 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 39 | V → D in BAB23893 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 69 | G → R in BAB23893 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 97 | G → A in AAA69475 (Ref. 3) Curated | 1 | |
Sequence conflicti | 113 | Q → H in BAB23893 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 124 | I → V in BAB23893 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 135 | K → S in BAB23893 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 182 | T → N (Ref. 3) Curated | 1 | |
Sequence conflicti | 182 | T → N (PubMed:9714804).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U51679 mRNA Translation: AAB01941.1 X82067 mRNA Translation: CAA57566.1 U20611 mRNA Translation: AAA69475.1 AF032722 , AF032718, AF032719, AF032720, AF032721 Genomic DNA Translation: AAC35744.1 AK005225 mRNA Translation: BAB23893.1 AK008433 mRNA Translation: BAB25666.1 AK010653 mRNA Translation: BAB27093.1 AK088280 mRNA Translation: BAC40255.1 BC002034 mRNA Translation: AAH02034.1 BC081454 mRNA Translation: AAH81454.1 |
CCDSi | CCDS40416.1 |
RefSeqi | NP_001304314.1, NM_001317385.1 NP_035693.3, NM_011563.6 |
Genome annotation databases
Ensembli | ENSMUST00000005292; ENSMUSP00000005292; ENSMUSG00000005161 ENSMUST00000109733; ENSMUSP00000105355; ENSMUSG00000005161 ENSMUST00000109734; ENSMUSP00000105356; ENSMUSG00000005161 ENSMUST00000164807; ENSMUSP00000126451; ENSMUSG00000005161 |
GeneIDi | 21672 |
KEGGi | mmu:21672 |
UCSCi | uc009mom.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U51679 mRNA Translation: AAB01941.1 X82067 mRNA Translation: CAA57566.1 U20611 mRNA Translation: AAA69475.1 AF032722 , AF032718, AF032719, AF032720, AF032721 Genomic DNA Translation: AAC35744.1 AK005225 mRNA Translation: BAB23893.1 AK008433 mRNA Translation: BAB25666.1 AK010653 mRNA Translation: BAB27093.1 AK088280 mRNA Translation: BAC40255.1 BC002034 mRNA Translation: AAH02034.1 BC081454 mRNA Translation: AAH81454.1 |
CCDSi | CCDS40416.1 |
RefSeqi | NP_001304314.1, NM_001317385.1 NP_035693.3, NM_011563.6 |
3D structure databases
SMRi | Q61171 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204094, 25 interactors |
IntActi | Q61171, 17 interactors |
MINTi | Q61171 |
STRINGi | 10090.ENSMUSP00000005292 |
Protein family/group databases
PeroxiBasei | 4474, Mm2CysPrx02 |
PTM databases
iPTMneti | Q61171 |
PhosphoSitePlusi | Q61171 |
SwissPalmi | Q61171 |
2D gel databases
REPRODUCTION-2DPAGEi | Q61171 |
SWISS-2DPAGEi | Q61171 |
UCD-2DPAGEi | Q61171 |
Proteomic databases
CPTACi | non-CPTAC-3401 |
EPDi | Q61171 |
jPOSTi | Q61171 |
MaxQBi | Q61171 |
PaxDbi | Q61171 |
PeptideAtlasi | Q61171 |
PRIDEi | Q61171 |
TopDownProteomicsi | Q61171 |
Protocols and materials databases
Antibodypediai | 3283, 538 antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 7001 |
MGIi | MGI:109486, Prdx2 |
Phylogenomic databases
eggNOGi | KOG0852, Eukaryota |
GeneTreei | ENSGT00940000155828 |
HOGENOMi | CLU_042529_21_0_1 |
InParanoidi | Q61171 |
OMAi | FAFTGVC |
OrthoDBi | 1326484at2759 |
PhylomeDBi | Q61171 |
TreeFami | TF105181 |
Enzyme and pathway databases
Reactomei | R-MMU-3299685, Detoxification of Reactive Oxygen Species R-MMU-5628897, TP53 Regulates Metabolic Genes |
Miscellaneous databases
BioGRID-ORCSi | 21672, 0 hits in 17 CRISPR screens |
ChiTaRSi | Prdx2, mouse |
PROi | PR:Q61171 |
RNActi | Q61171, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000005161, Expressed in cortical plate and 299 other tissues |
ExpressionAtlasi | Q61171, baseline and differential |
Genevisiblei | Q61171, MM |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRDX2_MOUSE | |
Accessioni | Q61171Primary (citable) accession number: Q61171 Secondary accession number(s): O88376 Q9DB49 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 198 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families