UniProtKB - Q61160 (FADD_MOUSE)
FAS-associated death domain protein
Fadd
Functioni
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.
By similarityGO - Molecular functioni
- caspase binding Source: UniProtKB
- death effector domain binding Source: MGI
- death receptor binding Source: MGI
- identical protein binding Source: MGI
- protease binding Source: MGI
- protein-containing complex binding Source: MGI
- receptor serine/threonine kinase binding Source: MGI
- tumor necrosis factor receptor binding Source: MGI
- tumor necrosis factor receptor superfamily binding Source: MGI
GO - Biological processi
- activation of cysteine-type endopeptidase activity Source: MGI
- apoptotic process Source: UniProtKB
- apoptotic signaling pathway Source: MGI
- behavioral response to cocaine Source: Ensembl
- cardiac muscle tissue development Source: UniProtKB
- cellular response to mechanical stimulus Source: Ensembl
- death-inducing signaling complex assembly Source: MGI
- defense response to virus Source: MGI
- extrinsic apoptotic signaling pathway Source: MGI
- extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
- innate immune response Source: UniProtKB
- kidney development Source: Ensembl
- lymph node development Source: UniProtKB
- motor neuron apoptotic process Source: MGI
- necroptotic signaling pathway Source: MGI
- negative regulation of activation-induced cell death of T cells Source: UniProtKB
- negative regulation of necroptotic process Source: MGI
- positive regulation of activated T cell proliferation Source: UniProtKB
- positive regulation of adaptive immune response Source: UniProtKB
- positive regulation of apoptotic process Source: MGI
- positive regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation Source: UniProtKB
- positive regulation of extrinsic apoptotic signaling pathway Source: MGI
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
- positive regulation of interferon-gamma production Source: UniProtKB
- positive regulation of interleukin-8 production Source: MGI
- positive regulation of macrophage differentiation Source: MGI
- positive regulation of proteolysis Source: MGI
- positive regulation of T cell mediated cytotoxicity Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of tumor necrosis factor production Source: MGI
- positive regulation of type I interferon-mediated signaling pathway Source: MGI
- regulation of necroptotic process Source: MGI
- response to morphine Source: MGI
- spleen development Source: UniProtKB
- T cell differentiation Source: UniProtKB
- T cell differentiation in thymus Source: UniProtKB
- T cell homeostasis Source: UniProtKB
- thymus development Source: UniProtKB
- TRAIL-activated apoptotic signaling pathway Source: MGI
Keywordsi
Biological process | Apoptosis, Immunity, Innate immunity |
Enzyme and pathway databases
Reactomei | R-MMU-140534, Caspase activation via Death Receptors in the presence of ligand R-MMU-2562578, TRIF-mediated programmed cell death R-MMU-3371378, Regulation by c-FLIP R-MMU-5218900, CASP8 activity is inhibited R-MMU-5357786, TNFR1-induced proapoptotic signaling R-MMU-5357905, Regulation of TNFR1 signaling R-MMU-5675482, Regulation of necroptotic cell death R-MMU-69416, Dimerization of procaspase-8 R-MMU-75157, FasL/ CD95L signaling R-MMU-75158, TRAIL signaling |
Names & Taxonomyi
Protein namesi | Recommended name: FAS-associated death domain protein1 PublicationAlternative name(s): FAS-associating death domain-containing protein1 Publication Mediator of receptor induced toxicity1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109324, Fadd |
VEuPathDBi | HostDB:ENSMUSG00000031077 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
- ripoptosome Source: UniProtKB
Nucleus
- nucleus Source: MGI
Plasma Membrane
- CD95 death-inducing signaling complex Source: MGI
- death-inducing signaling complex Source: MGI
- plasma membrane Source: MGI
Other locations
- cell body Source: MGI
- cytoplasm Source: MGI
- membrane raft Source: MGI
- neuron projection Source: MGI
- protein-containing complex Source: MGI
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191280 | 1 – 205 | FAS-associated death domain proteinAdd BLAST | 205 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 191 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q61160 |
jPOSTi | Q61160 |
MaxQBi | Q61160 |
PaxDbi | Q61160 |
PeptideAtlasi | Q61160 |
PRIDEi | Q61160 |
ProteomicsDBi | 271550 |
PTM databases
iPTMneti | Q61160 |
PhosphoSitePlusi | Q61160 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000031077, Expressed in primary oocyte and 247 other tissues |
Genevisiblei | Q61160, MM |
Interactioni
Subunit structurei
Can self-associate (By similarity).
Interacts with CFLAR, PEA15 and MBD4 (By similarity). When phosphorylated, part of a complex containing HIPK3 and FAS (By similarity). May interact with MAVS/IPS1 (By similarity).
Interacts with MOCV v-CFLAR protein and PIDD1 (By similarity).
Interacts (via death domain) with FAS (via death domain) (By similarity).
Interacts with CASP8 (PubMed:29440439).
Interacts directly (via DED domain) with NOL3 (via CARD domain); inhibits death-inducing signaling complex (DISC) assembly by inhibiting the increase in FAS-FADD binding induced by FAS activation (PubMed:15383280).
Interacts with RIPK1, TRADD and CASP8 (PubMed:28842570, PubMed:29440439, PubMed:30185824, PubMed:31519887).
Component of the AIM2 PANoptosome complex, a multiprotein complex that drives inflammatory cell death (PANoptosis) (PubMed:34471287).
Interacts with stimulated TNFRSF10B (By similarity).
By similarity6 PublicationsBinary interactionsi
Q61160
With | #Exp. | IntAct |
---|---|---|
Casp8 [O89110] | 6 | EBI-524415,EBI-851690 |
Fas [P25446] | 3 | EBI-524415,EBI-296206 |
Ripk1 [Q60855] | 8 | EBI-524415,EBI-529119 |
Ripk3 [Q9QZL0] | 6 | EBI-524415,EBI-2367423 |
GO - Molecular functioni
- caspase binding Source: UniProtKB
- death effector domain binding Source: MGI
- death receptor binding Source: MGI
- identical protein binding Source: MGI
- protease binding Source: MGI
- receptor serine/threonine kinase binding Source: MGI
- tumor necrosis factor receptor binding Source: MGI
- tumor necrosis factor receptor superfamily binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199586, 17 interactors |
ComplexPortali | CPX-1914, Ripoptosome |
DIPi | DIP-34771N |
IntActi | Q61160, 15 interactors |
MINTi | Q61160 |
STRINGi | 10090.ENSMUSP00000033394 |
Miscellaneous databases
RNActi | Q61160, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q61160 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q61160 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 81 | DEDPROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 97 – 181 | DeathPROSITE-ProRule annotationAdd BLAST | 85 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 181 – 205 | DisorderedSequence analysisAdd BLAST | 25 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 183 – 205 | Polar residuesSequence analysisAdd BLAST | 23 |
Domaini
Phylogenomic databases
eggNOGi | ENOG502S2RV, Eukaryota |
GeneTreei | ENSGT00390000002105 |
HOGENOMi | CLU_087961_0_0_1 |
InParanoidi | Q61160 |
OMAi | CKMNLVA |
OrthoDBi | 1619689at2759 |
PhylomeDBi | Q61160 |
TreeFami | TF102046 |
Family and domain databases
Gene3Di | 1.10.533.10, 2 hits |
InterProi | View protein in InterPro IPR011029, DEATH-like_dom_sf IPR000488, Death_domain IPR001875, DED_dom IPR016729, FADD |
PANTHERi | PTHR15077, PTHR15077, 1 hit |
Pfami | View protein in Pfam PF00531, Death, 1 hit PF01335, DED, 1 hit |
PIRSFi | PIRSF018586, FADD, 1 hit |
SMARTi | View protein in SMART SM00005, DEATH, 1 hit SM00031, DED, 1 hit |
SUPFAMi | SSF47986, SSF47986, 1 hit |
PROSITEi | View protein in PROSITE PS50017, DEATH_DOMAIN, 1 hit PS50168, DED, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDPFLVLLHS LSGSLSGNDL MELKFLCRER VSKRKLERVQ SGLDLFTVLL
60 70 80 90 100
EQNDLERGHT GLLRELLASL RRHDLLQRLD DFEAGTATAA PPGEADLQVA
110 120 130 140 150
FDIVCDNVGR DWKRLARELK VSEAKMDGIE EKYPRSLSER VRESLKVWKN
160 170 180 190 200
AEKKNASVAG LVKALRTCRL NLVADLVEEA QESVSKSENM SPVLRDSTVS
SSETP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 168 | C → F in AAA97876 (PubMed:8565075).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U50406 mRNA Translation: AAB07789.1 U43184 mRNA Translation: AAA97876.1 AK084808 mRNA Translation: BAC39283.1 AK169798 mRNA Translation: BAE41374.1 AK170927 mRNA Translation: BAE42120.1 BC004584 mRNA Translation: AAH04584.1 BC021400 mRNA Translation: AAH21400.1 |
CCDSi | CCDS22050.1 |
RefSeqi | NP_034305.1, NM_010175.5 |
Genome annotation databases
Ensembli | ENSMUST00000033394; ENSMUSP00000033394; ENSMUSG00000031077 |
GeneIDi | 14082 |
KEGGi | mmu:14082 |
UCSCi | uc009kql.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U50406 mRNA Translation: AAB07789.1 U43184 mRNA Translation: AAA97876.1 AK084808 mRNA Translation: BAC39283.1 AK169798 mRNA Translation: BAE41374.1 AK170927 mRNA Translation: BAE42120.1 BC004584 mRNA Translation: AAH04584.1 BC021400 mRNA Translation: AAH21400.1 |
CCDSi | CCDS22050.1 |
RefSeqi | NP_034305.1, NM_010175.5 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FAD | NMR | - | A | 89-183 | [»] | |
SMRi | Q61160 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 199586, 17 interactors |
ComplexPortali | CPX-1914, Ripoptosome |
DIPi | DIP-34771N |
IntActi | Q61160, 15 interactors |
MINTi | Q61160 |
STRINGi | 10090.ENSMUSP00000033394 |
PTM databases
iPTMneti | Q61160 |
PhosphoSitePlusi | Q61160 |
Proteomic databases
EPDi | Q61160 |
jPOSTi | Q61160 |
MaxQBi | Q61160 |
PaxDbi | Q61160 |
PeptideAtlasi | Q61160 |
PRIDEi | Q61160 |
ProteomicsDBi | 271550 |
Protocols and materials databases
Antibodypediai | 698, 1027 antibodies from 42 providers |
DNASUi | 14082 |
Genome annotation databases
Ensembli | ENSMUST00000033394; ENSMUSP00000033394; ENSMUSG00000031077 |
GeneIDi | 14082 |
KEGGi | mmu:14082 |
UCSCi | uc009kql.1, mouse |
Organism-specific databases
CTDi | 8772 |
MGIi | MGI:109324, Fadd |
VEuPathDBi | HostDB:ENSMUSG00000031077 |
Phylogenomic databases
eggNOGi | ENOG502S2RV, Eukaryota |
GeneTreei | ENSGT00390000002105 |
HOGENOMi | CLU_087961_0_0_1 |
InParanoidi | Q61160 |
OMAi | CKMNLVA |
OrthoDBi | 1619689at2759 |
PhylomeDBi | Q61160 |
TreeFami | TF102046 |
Enzyme and pathway databases
Reactomei | R-MMU-140534, Caspase activation via Death Receptors in the presence of ligand R-MMU-2562578, TRIF-mediated programmed cell death R-MMU-3371378, Regulation by c-FLIP R-MMU-5218900, CASP8 activity is inhibited R-MMU-5357786, TNFR1-induced proapoptotic signaling R-MMU-5357905, Regulation of TNFR1 signaling R-MMU-5675482, Regulation of necroptotic cell death R-MMU-69416, Dimerization of procaspase-8 R-MMU-75157, FasL/ CD95L signaling R-MMU-75158, TRAIL signaling |
Miscellaneous databases
BioGRID-ORCSi | 14082, 25 hits in 68 CRISPR screens |
ChiTaRSi | Fadd, mouse |
EvolutionaryTracei | Q61160 |
PROi | PR:Q61160 |
RNActi | Q61160, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031077, Expressed in primary oocyte and 247 other tissues |
Genevisiblei | Q61160, MM |
Family and domain databases
Gene3Di | 1.10.533.10, 2 hits |
InterProi | View protein in InterPro IPR011029, DEATH-like_dom_sf IPR000488, Death_domain IPR001875, DED_dom IPR016729, FADD |
PANTHERi | PTHR15077, PTHR15077, 1 hit |
Pfami | View protein in Pfam PF00531, Death, 1 hit PF01335, DED, 1 hit |
PIRSFi | PIRSF018586, FADD, 1 hit |
SMARTi | View protein in SMART SM00005, DEATH, 1 hit SM00031, DED, 1 hit |
SUPFAMi | SSF47986, SSF47986, 1 hit |
PROSITEi | View protein in PROSITE PS50017, DEATH_DOMAIN, 1 hit PS50168, DED, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FADD_MOUSE | |
Accessioni | Q61160Primary (citable) accession number: Q61160 Secondary accession number(s): Q3TC37, Q61082 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 183 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references