Bcar1

Functionⁱ

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration (By similarity). Has been shown to be essential in cardiovascular development during embryogenesis.By similarity

GO - Molecular functionⁱ

protein domain specific binding Source: MGI
protein kinase binding Source: MGI
SH3 domain binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

actin cytoskeleton organization Source: MGI
actin filament organization Source: UniProtKB
antigen receptor-mediated signaling pathway Source: UniProtKB
B cell receptor signaling pathway Source: UniProtKB
cell adhesion Source: UniProtKB-KW
cell chemotaxis Source: MGI
cell migration
Source: MGI
Inferred from direct assayⁱ
- 15728191
cellular response to endothelin Source: MGI
cellular response to hepatocyte growth factor stimulus Source: MGI
cellular response to nitric oxide Source: MGI
epidermal growth factor receptor signaling pathway Source: UniProtKB
G-protein coupled receptor signaling pathway
Source: UniProtKB
Inferred from direct assayⁱ
- 9083073
hepatocyte growth factor receptor signaling pathway Source: MGI
insulin receptor signaling pathway Source: UniProtKB
integrin-mediated signaling pathway Source: UniProtKB
negative regulation of substrate adhesion-dependent cell spreading Source: MGI
neurotrophin TRK receptor signaling pathway Source: UniProtKB
platelet-derived growth factor receptor signaling pathway
Source: UniProtKB
Inferred from direct assayⁱ
- 9083073
positive regulation of cell migration Source: UniProtKB
positive regulation of endothelial cell migration Source: MGI
vascular endothelial growth factor receptor signaling pathway Source: MGI

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Cell adhesion

Biological process

Cell adhesion

Enzyme and pathway databases

Reactomeⁱ	R-MMU-186763 Downstream signal transduction R-MMU-372708 p130Cas linkage to MAPK signaling for integrins R-MMU-4420097 VEGFA-VEGFR2 Pathway R-MMU-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases

Reactomeⁱ

R-MMU-186763 Downstream signal transduction
R-MMU-372708 p130Cas linkage to MAPK signaling for integrins
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Breast cancer anti-estrogen resistance protein 1 Alternative name(s): CRK-associated substrate p130cas
Gene namesⁱ	Name:Bcar1 Synonyms:Cas, Crkas
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 8

Organism-specific databases

MGIⁱ	MGI:108091 Bcar1

Keywords - Cellular componentⁱ

Cell junction, Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000064855	1 – 874	Breast cancer anti-estrogen resistance protein 1Add BLAST		874

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	1	N-acetylmethionineBy similarity	1
Modified residueⁱ	132	PhosphotyrosineCombined sources	1
Modified residueⁱ	143	PhosphoserineBy similarity	1
Modified residueⁱ	238	PhosphotyrosineCombined sources	1
Modified residueⁱ	253	PhosphotyrosineCombined sources	1
Modified residueⁱ	273	PhosphothreonineBy similarity	1
Modified residueⁱ	296	PhosphoserineBy similarity	1
Modified residueⁱ	366	PhosphotyrosineCombined sources	1
Modified residueⁱ	376	PhosphotyrosineCombined sources	1
Modified residueⁱ	414	PhosphotyrosineCombined sources	1
Modified residueⁱ	432	PhosphoserineBy similarity	1
Modified residueⁱ	441	PhosphoserineBy similarity	1
Modified residueⁱ	643	PhosphoserineBy similarity	1

Post-translational modificationⁱ

PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin mediated adhesion of cells to the extracellular matrix (By similarity).By similarity

Dephosphorylated by PTPN14 at Tyr-132.By similarity

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

PaxDbⁱ	Q61140
PRIDEⁱ	Q61140

PTM databases

iPTMnetⁱ	Q61140
PhosphoSitePlusⁱ	Q61140

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000031955 Expressed in 282 organ(s), highest expression level in heart
CleanExⁱ	MM_BCAR1
Genevisibleⁱ	Q61140 MM

Interactionⁱ

Subunit structureⁱ

Forms complexes in vivo with PTK2/FAK1, adapter protein CRKL and LYN kinase. Can heterodimerize with NEDD9. Interacts with activated CSPG4. Interacts with BMX, INPPL1/SHIP2 and PEAK1 (By similarity). Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas (By similarity). Interacts with BCAR3, the interaction regulates adhesion-dependent serine phosphorylation. Interacts with NPHP1, PTK2B/PYK2 and SH2D3C.By similarity6 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Nphp1	Q9QY53	2	EBI-77088,EBI-77230
Ptk2	P34152	3	EBI-77088,EBI-77070
PTPN1	P18031	5	EBI-77088,EBI-968788	From Homo sapiens.
Srcin1	Q9QWI6	2	EBI-77088,EBI-775592

GO - Molecular functionⁱ

protein domain specific binding Source: MGI
protein kinase binding Source: MGI
SH3 domain binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	198886, 8 interactors
CORUMⁱ	Q61140
IntActⁱ	Q61140, 14 interactors
Molecular INTeraction database More...MINTⁱ	Q61140
STRINGⁱ	10090.ENSMUSP00000129584

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	742 – 774	Combined sources	33
Helixⁱ	779 – 806	Combined sources	28
Helixⁱ	810 – 839	Combined sources	30
Helixⁱ	844 – 872	Combined sources	29

Show more details

3D structure databases

ProteinModelPortalⁱ	Q61140
SMRⁱ	Q61140
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	3 – 65	SH3PROSITE-ProRule annotationAdd BLAST		63

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	119 – 420	Substrate for kinasesBy similarityAdd BLAST		302
Regionⁱ	750 – 800	Divergent helix-loop-helix motifAdd BLAST		51

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	639 – 647	SH3-bindingSequence analysis		9

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	74 – 87	Pro-richAdd BLAST		14
Compositional biasⁱ	426 – 618	Ser-richAdd BLAST		193

Domainⁱ

Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.

A serine-rich region promotes activation of the serum response element (SRE).By similarity

The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK1.

Sequence similaritiesⁱ

Belongs to the CAS family.Curated

Keywords - Domainⁱ

SH3 domain, SH3-binding

Phylogenomic databases

eggNOGⁱ	ENOG410IEDD Eukaryota ENOG410ZSUM LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00490000043324
HOGENOMⁱ	HOG000261698
HOVERGENⁱ	HBG004354
InParanoidⁱ	Q61140
KEGG Orthology (KO) More...KOⁱ	K05726
OMAⁱ	TVEHNQP
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0RVA
TreeFamⁱ	TF328782

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd12001 SH3_BCAR1, 1 hit
Gene3Dⁱ	1.20.120.830, 1 hit
InterProⁱ	View protein in InterPro IPR028848 BCAR1 IPR035745 BCAR1_SH3 IPR021901 CAS_C IPR037362 CAS_fam IPR014928 Serine_rich_dom IPR038319 Serine_rich_sf IPR036028 SH3-like_dom_sf IPR001452 SH3_domain
PANTHERⁱ	PTHR10654 PTHR10654, 1 hit PTHR10654:SF15 PTHR10654:SF15, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF12026 DUF3513, 1 hit PF08824 Serine_rich, 1 hit PF00018 SH3_1, 1 hit
PRINTSⁱ	PR00452 SH3DOMAIN
SMARTⁱ	View protein in SMART SM00326 SH3, 1 hit
SUPFAMⁱ	SSF50044 SSF50044, 1 hit
PROSITEⁱ	View protein in PROSITE PS50002 SH3, 1 hit

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

Isoform Cas-B (identifier: Q61140-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVPNVLAKA LYDNVAESPD ELSFRKGDIM TVLERDTQGL DGWWLCSLHG 
        60         70         80         90        100
RQGIVPGNRL KILVGMYDKK PVGPGPGPPA TPPQPQPSLP QGVHAPVPPA 
       110        120        130        140        150
SQYSPMLPTA YQPQSDNVYL VPTPSKTQQG LYQAPGPNPQ FQSPPAKQTS 
       160        170        180        190        200
TFSKQTPHHS FPSPATDLYQ VPPGPGSPAQ DIYQVPPSAG IGHDIYQVPP 
       210        220        230        240        250
SLDTRGWEGT KPPAKVVVPT RVGQGYVYEA AQTEQDEYDT PRHLLAPGPQ 
       260        270        280        290        300
DIYDVPPVRG LLPNQYGQEV YDTPPMAVKG PNGRDPLLDV YDVPPSVEKG 
       310        320        330        340        350
LLSSSHHSVY DVPPSVSKDV PDGPLLREET YDVPPAFAKP KPFDPTRHPL 
       360        370        380        390        400
ILAAPPPDSP AAEDVYDVPP PAPDLYDVPP GLRRPGPGTL YDVPRERVLP 
       410        420        430        440        450
PEVADGSVVD DGVYAVPPPA EREAPTDGKR LSASSTGSTR SSQSASSLEV 
       460        470        480        490        500
VVPGREPLEL EVAVESLARL QQGVSTTVAH LLDLVGSASG PGGWRGTSEP 
       510        520        530        540        550
QEPPAQDLKA AVAAVHGAVH ELLEFARGAV SNATHTSDRT LHAKLSRQLQ 
       560        570        580        590        600
KMEDVYQTLV VHGQVLDSGR GSPGFTPEDL DRLVACSRAV PEDAKQLASF 
       610        620        630        640        650
LHGNASLLFR RTKAPGPGPE GSSSLHPNPT DKASSIQSRP LPSPPKFTSQ 
       660        670        680        690        700
DSPDGQYENS EGGWMEDYDY VHLQGKEEFE KTQKELLERG NIMRQGKGQL 
       710        720        730        740        750
ELQQLKQFER LEQEVSRPID HDLANWTPAQ PLVPGRTGGL GPSDRQLLLF 
       760        770        780        790        800
YLEQCEANLT TLTDAVDAFF TAVATNQPPK IFVAHSKFVI LSAHKLVFIG 
       810        820        830        840        850
DTLSRQAKAA DVRSQVTHYS NLLCDLLRGI VATTKAAALQ YPSPSAAQDM 
       860        870 
VDRVKELGHS TQQFRRVLGQ LAAA

Length:874

Mass (Da):94,285

Last modified:July 27, 2011 - v2

Checksum:ⁱ81E56BC7AD87B095

GO

Isoform Cas-A (identifier: Q61140-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-4: MTVP → MKYL

« Hide

        10         20         30         40         50
MKYLNVLAKA LYDNVAESPD ELSFRKGDIM TVLERDTQGL DGWWLCSLHG 
        60         70         80         90        100
RQGIVPGNRL KILVGMYDKK PVGPGPGPPA TPPQPQPSLP QGVHAPVPPA 
       110        120        130        140        150
SQYSPMLPTA YQPQSDNVYL VPTPSKTQQG LYQAPGPNPQ FQSPPAKQTS 
       160        170        180        190        200
TFSKQTPHHS FPSPATDLYQ VPPGPGSPAQ DIYQVPPSAG IGHDIYQVPP 
       210        220        230        240        250
SLDTRGWEGT KPPAKVVVPT RVGQGYVYEA AQTEQDEYDT PRHLLAPGPQ 
       260        270        280        290        300
DIYDVPPVRG LLPNQYGQEV YDTPPMAVKG PNGRDPLLDV YDVPPSVEKG 
       310        320        330        340        350
LLSSSHHSVY DVPPSVSKDV PDGPLLREET YDVPPAFAKP KPFDPTRHPL 
       360        370        380        390        400
ILAAPPPDSP AAEDVYDVPP PAPDLYDVPP GLRRPGPGTL YDVPRERVLP 
       410        420        430        440        450
PEVADGSVVD DGVYAVPPPA EREAPTDGKR LSASSTGSTR SSQSASSLEV 
       460        470        480        490        500
VVPGREPLEL EVAVESLARL QQGVSTTVAH LLDLVGSASG PGGWRGTSEP 
       510        520        530        540        550
QEPPAQDLKA AVAAVHGAVH ELLEFARGAV SNATHTSDRT LHAKLSRQLQ 
       560        570        580        590        600
KMEDVYQTLV VHGQVLDSGR GSPGFTPEDL DRLVACSRAV PEDAKQLASF 
       610        620        630        640        650
LHGNASLLFR RTKAPGPGPE GSSSLHPNPT DKASSIQSRP LPSPPKFTSQ 
       660        670        680        690        700
DSPDGQYENS EGGWMEDYDY VHLQGKEEFE KTQKELLERG NIMRQGKGQL 
       710        720        730        740        750
ELQQLKQFER LEQEVSRPID HDLANWTPAQ PLVPGRTGGL GPSDRQLLLF 
       760        770        780        790        800
YLEQCEANLT TLTDAVDAFF TAVATNQPPK IFVAHSKFVI LSAHKLVFIG 
       810        820        830        840        850
DTLSRQAKAA DVRSQVTHYS NLLCDLLRGI VATTKAAALQ YPSPSAAQDM 
       860        870 
VDRVKELGHS TQQFRRVLGQ LAAA

Show »

Length:874

Mass (Da):94,392

Checksum:ⁱ25B8F5D1D61CC8B1

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	72	V → A in AAA93381 (PubMed:7479864).Curated		1
Sequence conflictⁱ	72	V → A in AAA93248 (PubMed:7479864).Curated		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_004134	1 – 4	MTVP → MKYL in isoform Cas-A. 1 Publication		4

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U48853 mRNA Translation: AAA93381.1 U28151 mRNA Translation: AAA93248.1 AK145863 mRNA Translation: BAE26705.1 BC057578 mRNA Translation: AAH57578.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS52675.1 [Q61140-1] CCDS85616.1 [Q61140-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_001185768.1, NM_001198839.1 [Q61140-2] NP_034084.2, NM_009954.3 [Q61140-1]
UniGeneⁱ	Mm.3758

Genome annotation databases

Ensemblⁱ	ENSMUST00000166232; ENSMUSP00000129584; ENSMUSG00000031955 [Q61140-1] ENSMUST00000212349; ENSMUSP00000148364; ENSMUSG00000031955 [Q61140-2]
GeneIDⁱ	12927
KEGGⁱ	mmu:12927
UCSC genome browser More...UCSCⁱ	uc009nmt.2 mouse [Q61140-2] uc009nmu.2 mouse [Q61140-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q61140	Breast cancer anti-estrogen resistance protein 1	)	HUMAN		870	UniRef90_P56945
BCAR1, Cas family scaffolding protein		OTOGA		920
BCAR1 isoform 5		PANTR		916
BCAR1, Cas family scaffolding protein		PANPA		916
BCAR1, Cas family scaffolding protein		PROCO		902
+282

Protein	Similar proteins		Species	Score	Length	Source
Q61140	Breast cancer anti-estrogen resistance protein 1	)	HUMAN		870	UniRef50_P56945
Breast cancer anti-estrogen resistance protein 1	)	RAT		968
BCAR1, Cas family scaffolding protein		OTOGA		920
BCAR1 isoform 5		PANTR		916
BCAR1, Cas family scaffolding protein		PANPA		916
+506

Entry informationⁱ

Entry nameⁱ	BCAR1_MOUSE
Accessionⁱ	Q61140Primary (citable) accession number: Q61140 Secondary accession number(s): Q60869, Q6PFF9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
	Last sequence update:	July 27, 2011
	Last modified:	September 12, 2018
	This is version 173 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q61140 (BCAR1_MOUSE)

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Breast cancer anti-estrogen resistance protein 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cytoskeleton

Cytosol

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Motif

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ