UniProtKB - Q61140 (BCAR1_MOUSE)
Breast cancer anti-estrogen resistance protein 1
Bcar1
Functioni
GO - Molecular functioni
- protein domain specific binding Source: MGI
- protein kinase binding Source: MGI
- SH3 domain binding Source: UniProtKB-KW
GO - Biological processi
- actin cytoskeleton organization Source: MGI
- actin filament organization Source: UniProtKB
- actin filament reorganization Source: GO_Central
- antigen receptor-mediated signaling pathway Source: UniProtKB
- B cell receptor signaling pathway Source: UniProtKB
- cell adhesion Source: UniProtKB-KW
- cell chemotaxis Source: MGI
- cell migration Source: MGI
- cellular response to endothelin Source: MGI
- cellular response to hepatocyte growth factor stimulus Source: MGI
- cellular response to nitric oxide Source: MGI
- endothelin receptor signaling pathway Source: UniProtKB
- epidermal growth factor receptor signaling pathway Source: UniProtKB
- G protein-coupled receptor signaling pathway Source: UniProtKB
- hepatocyte growth factor receptor signaling pathway Source: MGI
- insulin receptor signaling pathway Source: UniProtKB
- integrin-mediated signaling pathway Source: UniProtKB
- negative regulation of substrate adhesion-dependent cell spreading Source: MGI
- neurotrophin TRK receptor signaling pathway Source: UniProtKB
- platelet-derived growth factor receptor signaling pathway Source: UniProtKB
- positive regulation of cell migration Source: UniProtKB
- positive regulation of endothelial cell migration Source: MGI
- transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
- vascular endothelial growth factor receptor signaling pathway Source: MGI
Keywordsi
Biological process | Cell adhesion |
Enzyme and pathway databases
Reactomei | R-MMU-186763, Downstream signal transduction R-MMU-372708, p130Cas linkage to MAPK signaling for integrins R-MMU-4420097, VEGFA-VEGFR2 Pathway R-MMU-8849471, PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases |
Names & Taxonomyi
Protein namesi | Recommended name: Breast cancer anti-estrogen resistance protein 1Alternative name(s): CRK-associated substrate p130cas |
Gene namesi | Name:Bcar1 Synonyms:Cas, Crkas |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:108091, Bcar1 |
Subcellular locationi
Other locations
- focal adhesion 1 Publication
- Cytoplasm 1 Publication
- axon 1 Publication
Note: Unphosphorylated form localizes in the cytoplasm (PubMed:22801373). Localizes to focal adhesion sites following integrin engagement (PubMed:22801373).1 Publication
Cytoskeleton
- actin cytoskeleton Source: MGI
Cytosol
- cytosol Source: Reactome
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- axon Source: UniProtKB-SubCell
- cytoplasm Source: UniProtKB
- focal adhesion Source: MGI
- lamellipodium Source: MGI
- membrane Source: UniProtKB
- ruffle Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cell projection, CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064855 | 1 – 874 | Breast cancer anti-estrogen resistance protein 1Add BLAST | 874 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 132 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 143 | PhosphoserineBy similarity | 1 | |
Modified residuei | 238 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 253 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 273 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 296 | PhosphoserineBy similarity | 1 | |
Modified residuei | 366 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 376 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 414 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 432 | PhosphoserineBy similarity | 1 | |
Modified residuei | 441 | PhosphoserineBy similarity | 1 | |
Modified residuei | 643 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q61140 |
PRIDEi | Q61140 |
PTM databases
iPTMneti | Q61140 |
PhosphoSitePlusi | Q61140 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000031955, Expressed in heart and 301 other tissues |
Genevisiblei | Q61140, MM |
Interactioni
Subunit structurei
Forms complexes in vivo with PTK2/FAK1, adapter protein CRKL and LYN kinase. Can heterodimerize with NEDD9.
Component of a complex comprised of SH2D3C, BCAR1/CAS, and CRK (By similarity). Within the complex, interacts with SH2D3C (via C-terminus), and CRK (By similarity).
Part of a complex comprised of PTPRA, BCAR1, BCAR3 (via SH2 domain) and SRC; the formation of the complex is dependent on intergrin mediated-tyrosine phosphorylation of PTPRA (PubMed:22801373).
Interacts with BCAR3 (via Ras-GEF domain); the interaction regulates adhesion-dependent serine phosphorylation (PubMed:10896938, PubMed:22801373).
Interacts with SMAD2 and SMAD3 (PubMed:25499443).
Interacts with NPHP1 (PubMed:10739664).
Interacts with PTK2B/PYK2 (By similarity).
Interacts (via C-terminus) with SH2D3C/CHAT isoform 2 (via C-terminus) (PubMed:10692442, PubMed:12486027, PubMed:17174122).
Interacts with activated CSPG4.
Interacts with BMX, INPPL1/SHIP2 and PEAK1 (By similarity).
Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas (By similarity).
Interacts with TNK2 via SH3 domains.
By similarity9 PublicationsBinary interactionsi
Hide detailsQ61140
With | #Exp. | IntAct |
---|---|---|
Nphp1 [Q9QY53] | 2 | EBI-77088,EBI-77230 |
Ptk2 [P34152] | 3 | EBI-77088,EBI-77070 |
Srcin1 [Q9QWI6] | 2 | EBI-77088,EBI-775592 |
PTPN1 [P18031] from Homo sapiens. | 5 | EBI-77088,EBI-968788 |
GO - Molecular functioni
- protein domain specific binding Source: MGI
- protein kinase binding Source: MGI
- SH3 domain binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 198886, 14 interactors |
CORUMi | Q61140 |
IntActi | Q61140, 14 interactors |
MINTi | Q61140 |
STRINGi | 10090.ENSMUSP00000129584 |
Miscellaneous databases
RNActi | Q61140, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q61140 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 65 | SH3PROSITE-ProRule annotationAdd BLAST | 63 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 119 – 420 | Substrate for kinasesBy similarityAdd BLAST | 302 | |
Regioni | 750 – 800 | Divergent helix-loop-helix motifAdd BLAST | 51 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 639 – 647 | SH3-bindingSequence analysis | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 74 – 87 | Pro-richAdd BLAST | 14 | |
Compositional biasi | 426 – 618 | Ser-richAdd BLAST | 193 |
Domaini
Sequence similaritiesi
Keywords - Domaini
SH3 domain, SH3-bindingPhylogenomic databases
eggNOGi | ENOG502QQHE, Eukaryota |
GeneTreei | ENSGT00950000183008 |
HOGENOMi | CLU_017000_1_0_1 |
InParanoidi | Q61140 |
OMAi | NYADTPD |
OrthoDBi | 1086228at2759 |
TreeFami | TF328782 |
Family and domain databases
CDDi | cd12001, SH3_BCAR1, 1 hit |
Gene3Di | 1.20.120.830, 1 hit |
InterProi | View protein in InterPro IPR028848, BCAR1 IPR035745, BCAR1_SH3 IPR021901, CAS_C IPR037362, CAS_fam IPR014928, Serine_rich_dom IPR038319, Serine_rich_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
PANTHERi | PTHR10654, PTHR10654, 1 hit PTHR10654:SF15, PTHR10654:SF15, 1 hit |
Pfami | View protein in Pfam PF12026, CAS_C, 1 hit PF08824, Serine_rich, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTVPNVLAKA LYDNVAESPD ELSFRKGDIM TVLERDTQGL DGWWLCSLHG
60 70 80 90 100
RQGIVPGNRL KILVGMYDKK PVGPGPGPPA TPPQPQPSLP QGVHAPVPPA
110 120 130 140 150
SQYSPMLPTA YQPQSDNVYL VPTPSKTQQG LYQAPGPNPQ FQSPPAKQTS
160 170 180 190 200
TFSKQTPHHS FPSPATDLYQ VPPGPGSPAQ DIYQVPPSAG IGHDIYQVPP
210 220 230 240 250
SLDTRGWEGT KPPAKVVVPT RVGQGYVYEA AQTEQDEYDT PRHLLAPGPQ
260 270 280 290 300
DIYDVPPVRG LLPNQYGQEV YDTPPMAVKG PNGRDPLLDV YDVPPSVEKG
310 320 330 340 350
LLSSSHHSVY DVPPSVSKDV PDGPLLREET YDVPPAFAKP KPFDPTRHPL
360 370 380 390 400
ILAAPPPDSP AAEDVYDVPP PAPDLYDVPP GLRRPGPGTL YDVPRERVLP
410 420 430 440 450
PEVADGSVVD DGVYAVPPPA EREAPTDGKR LSASSTGSTR SSQSASSLEV
460 470 480 490 500
VVPGREPLEL EVAVESLARL QQGVSTTVAH LLDLVGSASG PGGWRGTSEP
510 520 530 540 550
QEPPAQDLKA AVAAVHGAVH ELLEFARGAV SNATHTSDRT LHAKLSRQLQ
560 570 580 590 600
KMEDVYQTLV VHGQVLDSGR GSPGFTPEDL DRLVACSRAV PEDAKQLASF
610 620 630 640 650
LHGNASLLFR RTKAPGPGPE GSSSLHPNPT DKASSIQSRP LPSPPKFTSQ
660 670 680 690 700
DSPDGQYENS EGGWMEDYDY VHLQGKEEFE KTQKELLERG NIMRQGKGQL
710 720 730 740 750
ELQQLKQFER LEQEVSRPID HDLANWTPAQ PLVPGRTGGL GPSDRQLLLF
760 770 780 790 800
YLEQCEANLT TLTDAVDAFF TAVATNQPPK IFVAHSKFVI LSAHKLVFIG
810 820 830 840 850
DTLSRQAKAA DVRSQVTHYS NLLCDLLRGI VATTKAAALQ YPSPSAAQDM
860 870
VDRVKELGHS TQQFRRVLGQ LAAA
The sequence of this isoform differs from the canonical sequence as follows:
1-4: MTVP → MKYL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 72 | V → A in AAA93381 (PubMed:7479864).Curated | 1 | |
Sequence conflicti | 72 | V → A in AAA93248 (PubMed:7479864).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004134 | 1 – 4 | MTVP → MKYL in isoform Cas-A. 1 Publication | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U48853 mRNA Translation: AAA93381.1 U28151 mRNA Translation: AAA93248.1 AK145863 mRNA Translation: BAE26705.1 BC057578 mRNA Translation: AAH57578.1 |
CCDSi | CCDS52675.1 [Q61140-1] CCDS85616.1 [Q61140-2] |
RefSeqi | NP_001185768.1, NM_001198839.1 [Q61140-2] NP_034084.2, NM_009954.3 [Q61140-1] |
Genome annotation databases
Ensembli | ENSMUST00000166232; ENSMUSP00000129584; ENSMUSG00000031955 [Q61140-1] ENSMUST00000212349; ENSMUSP00000148364; ENSMUSG00000031955 [Q61140-2] |
GeneIDi | 12927 |
KEGGi | mmu:12927 |
UCSCi | uc009nmt.2, mouse [Q61140-2] uc009nmu.2, mouse [Q61140-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U48853 mRNA Translation: AAA93381.1 U28151 mRNA Translation: AAA93248.1 AK145863 mRNA Translation: BAE26705.1 BC057578 mRNA Translation: AAH57578.1 |
CCDSi | CCDS52675.1 [Q61140-1] CCDS85616.1 [Q61140-2] |
RefSeqi | NP_001185768.1, NM_001198839.1 [Q61140-2] NP_034084.2, NM_009954.3 [Q61140-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5W93 | X-ray | 2.00 | A/B/C | 738-874 | [»] | |
SMRi | Q61140 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198886, 14 interactors |
CORUMi | Q61140 |
IntActi | Q61140, 14 interactors |
MINTi | Q61140 |
STRINGi | 10090.ENSMUSP00000129584 |
PTM databases
iPTMneti | Q61140 |
PhosphoSitePlusi | Q61140 |
Proteomic databases
PaxDbi | Q61140 |
PRIDEi | Q61140 |
Protocols and materials databases
Antibodypediai | 3607, 774 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000166232; ENSMUSP00000129584; ENSMUSG00000031955 [Q61140-1] ENSMUST00000212349; ENSMUSP00000148364; ENSMUSG00000031955 [Q61140-2] |
GeneIDi | 12927 |
KEGGi | mmu:12927 |
UCSCi | uc009nmt.2, mouse [Q61140-2] uc009nmu.2, mouse [Q61140-1] |
Organism-specific databases
CTDi | 9564 |
MGIi | MGI:108091, Bcar1 |
Phylogenomic databases
eggNOGi | ENOG502QQHE, Eukaryota |
GeneTreei | ENSGT00950000183008 |
HOGENOMi | CLU_017000_1_0_1 |
InParanoidi | Q61140 |
OMAi | NYADTPD |
OrthoDBi | 1086228at2759 |
TreeFami | TF328782 |
Enzyme and pathway databases
Reactomei | R-MMU-186763, Downstream signal transduction R-MMU-372708, p130Cas linkage to MAPK signaling for integrins R-MMU-4420097, VEGFA-VEGFR2 Pathway R-MMU-8849471, PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases |
Miscellaneous databases
BioGRID-ORCSi | 12927, 0 hits in 19 CRISPR screens |
ChiTaRSi | Bcar1, mouse |
PROi | PR:Q61140 |
RNActi | Q61140, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031955, Expressed in heart and 301 other tissues |
Genevisiblei | Q61140, MM |
Family and domain databases
CDDi | cd12001, SH3_BCAR1, 1 hit |
Gene3Di | 1.20.120.830, 1 hit |
InterProi | View protein in InterPro IPR028848, BCAR1 IPR035745, BCAR1_SH3 IPR021901, CAS_C IPR037362, CAS_fam IPR014928, Serine_rich_dom IPR038319, Serine_rich_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
PANTHERi | PTHR10654, PTHR10654, 1 hit PTHR10654:SF15, PTHR10654:SF15, 1 hit |
Pfami | View protein in Pfam PF12026, CAS_C, 1 hit PF08824, Serine_rich, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BCAR1_MOUSE | |
Accessioni | Q61140Primary (citable) accession number: Q61140 Secondary accession number(s): Q60869, Q6PFF9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 188 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families