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Protein

NGFI-A-binding protein 2

Gene

Nab2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.

GO - Molecular functioni

  • identical protein binding Source: MGI
  • transcription factor binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionRepressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
NGFI-A-binding protein 2
Alternative name(s):
EGR-1-binding protein 2
Gene namesi
Name:Nab2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:107563 Nab2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000770431 – 525NGFI-A-binding protein 2Add BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphoserineBy similarity1
Modified residuei157PhosphoserineCombined sources1
Modified residuei159PhosphoserineCombined sources1
Modified residuei162PhosphoserineCombined sources1
Modified residuei171PhosphoserineCombined sources1
Cross-linki379Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei479PhosphoserineCombined sources1
Cross-linki517Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki517Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity

Post-translational modificationi

Sumoylation by EGR2 represses EGR2 transcriptional activity in hindbrain.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61127
PaxDbiQ61127
PeptideAtlasiQ61127
PRIDEiQ61127

PTM databases

iPTMnetiQ61127
PhosphoSitePlusiQ61127

Expressioni

Tissue specificityi

Highly expressed in brain and thymus, and at lower levels in spleen, kidney, heart and testis. Isoform 1 is predominantly expressed in testis, whereas isoform 3 is more abundant in thymus.

Inductioni

By serum stimulation.

Gene expression databases

BgeeiENSMUSG00000025402 Expressed in 251 organ(s), highest expression level in skin epidermis
CleanExiMM_NAB2
ExpressionAtlasiQ61127 baseline and differential
GenevisibleiQ61127 MM

Interactioni

Subunit structurei

Homomultimers may associate with EGR1 bound to DNA.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201681, 3 interactors
IntActiQ61127, 4 interactors
MINTiQ61127
STRINGi10090.ENSMUSP00000026469

Structurei

3D structure databases

ProteinModelPortaliQ61127
SMRiQ61127
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 113NCD1Add BLAST79
Regioni267 – 356NCD2Add BLAST90
Regioni353 – 384Necessary for nuclear localizationBy similarityAdd BLAST32

Domaini

The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.

Sequence similaritiesi

Belongs to the NAB family.Curated

Phylogenomic databases

eggNOGiKOG3835 Eukaryota
ENOG410YKFH LUCA
GeneTreeiENSGT00390000006330
HOGENOMiHOG000026777
HOVERGENiHBG003127
InParanoidiQ61127
KOiK22388
OMAiHARTNAD
OrthoDBiEOG091G0NQE
TreeFamiTF315501

Family and domain databases

Gene3Di3.40.390.60, 1 hit
InterProiView protein in InterPro
IPR006989 NAB_co-repressor_dom
IPR039040 NAB_fam
IPR006988 Nab_N
IPR038398 NCD2_sf
PANTHERiPTHR12623 PTHR12623, 1 hit
PfamiView protein in Pfam
PF04904 NCD1, 1 hit
PF04905 NCD2, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD342192 Nab_N, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q61127-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MHRAPSPTAE QPPGRGDNTR RTPQPRFKAS APAMALPRTL GELQLYRVLQ
60 70 80 90 100
RANLLSYYET FIQQGGDDVQ QLCEAGEEEF LEIMALVGMA TKPLHVRRLQ
110 120 130 140 150
KALREWATNP GLFSQPVPAV PVSSIPLFKI SETAGTRKGS MSNGHGSPGE
160 170 180 190 200
KAGSARSFSP KSPLELGEKL SPLPGGPGAG DPRIWPGQST PESDVGAGGE
210 220 230 240 250
EEAGSPPFSP PAGGGVSEGP GVGGVAAGGA GGGPDRLEPE MVRMVVESVE
260 270 280 290 300
RIFRSFPRGD TGEIASLLKL NKKLARSVGH IFEMDDHDAQ KEEEIRKYSV
310 320 330 340 350
IYGRLDSKRR EGKQLSLHEL TINEAAAQFC MRDNTLLLRR VELFSLSRQV
360 370 380 390 400
ARESTYLSSL KGSRLHSEEL GGPPLKKLKQ EVGEQSHNEI QQPPPGPESY
410 420 430 440 450
APPYRPSLEE DSASLSGESL DGHLQAVGSC PRLTPPPADL PLALPAHGLW
460 470 480 490 500
SRHILQQTLM DEGLRLARLV SHDRVGRLSP CVPAKPPLAE FEEGLLDRCP
510 520
APGPHPALVE GRRSSVKVEA EASRQ
Length:525
Mass (Da):56,577
Last modified:July 27, 2011 - v2
Checksum:i4FFA510C8E39DF70
GO
Isoform 2 (identifier: Q61127-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     320-322: LTI → ASL
     323-525: Missing.

Show »
Length:322
Mass (Da):34,376
Checksum:i61C0C1BAF842719D
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3TYF1Q3TYF1_MOUSE
NGFI-A-binding protein 2
Nab2
461Annotation score:
D3Z292D3Z292_MOUSE
NGFI-A-binding protein 2
Nab2
66Annotation score:
D3Z008D3Z008_MOUSE
NGFI-A-binding protein 2
Nab2
46Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti233G → C in AAC52650 (PubMed:8668170).Curated1
Sequence conflicti465R → W in AAC52650 (PubMed:8668170).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003388320 – 322LTI → ASL in isoform 2. 1 Publication3
Alternative sequenceiVSP_003389323 – 525Missing in isoform 2. 1 PublicationAdd BLAST203

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47543 mRNA Translation: AAC52650.1
CH466578 Genomic DNA Translation: EDL24515.1
BC045139 mRNA Translation: AAH45139.1
CCDSiCCDS24247.1 [Q61127-1]
RefSeqiNP_001116367.1, NM_001122895.1
NP_032694.2, NM_008668.2 [Q61127-1]
UniGeneiMm.336898
Mm.470656

Genome annotation databases

EnsembliENSMUST00000026469; ENSMUSP00000026469; ENSMUSG00000025402 [Q61127-1]
GeneIDi17937
KEGGimmu:17937
UCSCiuc011xpt.1 mouse [Q61127-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNAB2_MOUSE
AccessioniPrimary (citable) accession number: Q61127
Secondary accession number(s): Q80VR9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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