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Protein

Zinc finger protein ubi-d4

Gene

Dpf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an active role in transcriptional regulation by binding modified histones H3 and H4. Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells (By similarity). Might also have a role in the development and maturation of lymphoid cells (PubMed:7961935). Involved in the regulation of non-canonical NF-kappa-B pathway (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri209 – 232C2H2-typePROSITE-ProRule annotationAdd BLAST24
Zinc fingeri270 – 330PHD-type 1PROSITE-ProRule annotationAdd BLAST61
Zinc fingeri327 – 377PHD-type 2PROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

  • histone acetyltransferase activity Source: GO_Central
  • histone binding Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • nucleic acid binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator
Biological processApoptosis, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein ubi-d4
Alternative name(s):
Apoptosis response zinc finger protein
BRG1-associated factor 45D
Short name:
BAF45D
D4, zinc and double PHD fingers family 2
Protein requiem
Gene namesi
Name:Dpf2
Synonyms:Baf45d, Req, Ubid4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:109529 Dpf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001681502 – 391Zinc finger protein ubi-d4Add BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki10Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki99Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki107Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki108Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei142PhosphoserineCombined sources1
Modified residuei172PhosphotyrosineBy similarity1
Modified residuei176PhosphothreonineCombined sources1
Cross-linki178Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki196Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei200PhosphoserineBy similarity1
Modified residuei244PhosphoserineBy similarity1
Modified residuei280PhosphoserineBy similarity1
Cross-linki281Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ61103
MaxQBiQ61103
PaxDbiQ61103
PeptideAtlasiQ61103
PRIDEiQ61103

PTM databases

iPTMnetiQ61103
PhosphoSitePlusiQ61103

Expressioni

Tissue specificityi

In embryo, highest levels are seen in brain, eyes, thymus and olfactory epithelium in nose, whereas several other tissues, including the musculoskeletal system, show moderate expression. In adult, higher expression in testis, medium in thymus and spleen, lower in certain parts of the brain as the hippocampus. No expression in adult heart, lung, liver, duodenum and kidney.1 Publication

Developmental stagei

Already detected at embryonic day 8.5. Expressed ubiquitously throughout the developing spinal cord, brain and other embryonic tissues at E10.5-E16.5.1 Publication

Gene expression databases

BgeeiENSMUSG00000024826 Expressed in 327 organ(s), highest expression level in ear vesicle
CleanExiMM_DPF2
ExpressionAtlasiQ61103 baseline and differential
GenevisibleiQ61103 MM

Interactioni

Subunit structurei

Interacts with the nucleosomes, in particular nucleosomes bearing histone H3 crotonylated at 'Lys-14' (H3K14cr) for which DPF2 has high affinity. Also interacts (via PHD-type zinc finger domains) with histone H3 butyrylated at 'Lys-14' (H3K14bu), histone H3 propionylated at 'Lys-14' (H3K14pr), and histone H3 acetylated at 'Lys-14' (H3K14ac). Interacts with histone H3 acetylated at 'Lys-9' (H3K9ac), histone H3 di-methylated at 'Lys-9' (H3K9me2), and histone H3 tri-methylated at 'Lys-9' (H3K9me3). Interacts with histone H4 acetylated at 'Lys-12' (H4K12ac). Interacts with histone H4 acetylated at 'Lys-16' (H4K16ac). Interacts with SWI/SNF complex components. Interacts with SMARCA2, SMARCA4, SMARCB1 and SMARCD1. Interacts with SMARCC1, SMARCC2 and ACTL6A. Interacts with RUNX1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202862, 10 interactors
ComplexPortaliCPX-1251 Embryonic stem cell-specific SWI/SNF ATP-dependent chromatin remodeling complex
DIPiDIP-59247N
IntActiQ61103, 7 interactors
MINTiQ61103
STRINGi10090.ENSMUSP00000120125

Structurei

3D structure databases

ProteinModelPortaliQ61103
SMRiQ61103
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the requiem/DPF family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri209 – 232C2H2-typePROSITE-ProRule annotationAdd BLAST24
Zinc fingeri270 – 330PHD-type 1PROSITE-ProRule annotationAdd BLAST61
Zinc fingeri327 – 377PHD-type 2PROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1244 Eukaryota
ENOG410YDAG LUCA
GeneTreeiENSGT00930000150839
HOVERGENiHBG004475
InParanoidiQ61103
KOiK13196
PhylomeDBiQ61103
TreeFamiTF318971

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR025750 Requiem/DPF_N_dom
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF00628 PHD, 1 hit
PF14051 Requiem_N, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 2 hits
SM00355 ZnF_C2H2, 1 hit
SUPFAMiSSF57667 SSF57667, 1 hit
SSF57903 SSF57903, 2 hits
PROSITEiView protein in PROSITE
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 2 hits
PS00028 ZINC_FINGER_C2H2_1, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q61103-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAVVENVVK LLGEQYYKDA MEQCHNYNAR LCAERSVRLP FLDSQTGVAQ
60 70 80 90 100
SNCYIWMEKR HRGPGLASGQ LYSYPARRWR KKRRAHPPED PRLSFPSIKP
110 120 130 140 150
DTDQTLKKEG LISQDGSSLE ALLRTDPLEK RGAPDPRVDD DSLGEFPVSN
160 170 180 190 200
SRARKRIIEP DDFLDDLDDE DYEEDTPKRR GKGKSKSKGV SSARKKLDAS
210 220 230 240 250
ILEDRDKPYA CDICGKRYKN RPGLSYHYAH SHLAEEEGED KEDSRPPTPV
260 270 280 290 300
SQRSEEQKSK KGPDGLALPN NYCDFCLGDS KINKKTGQPE ELVSCSDCGR
310 320 330 340 350
SGHPSCLQFT PVMMAAVKTY RWQCIECKCC NLCGTSENDD QLLFCDDCDR
360 370 380 390
GYHMYCLTPS MSEPPEGSWS CHLCLDLLKE KASIYQNQNS S
Length:391
Mass (Da):44,230
Last modified:November 1, 1996 - v1
Checksum:i25598DBC80915BF2
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z5N6D3Z5N6_MOUSE
Zinc finger protein ubi-d4
Dpf2
405Annotation score:
F8WIP7F8WIP7_MOUSE
Zinc finger protein ubi-d4
Dpf2
214Annotation score:

Sequence cautioni

The sequence AAA64637 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH07188 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti176T → R in AAA64637 (PubMed:7961935).Curated1
Sequence conflicti187 – 188Missing in AAH07188 (PubMed:15489334).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43921 mRNA Translation: AAC52783.1
AF108134 Genomic DNA Translation: AAF21306.1
AK004812 mRNA Translation: BAB23583.1
AK138047 mRNA Translation: BAE23543.1
AK144106 mRNA Translation: BAE25702.1
AK144848 mRNA Translation: BAE26098.1
AK144954 mRNA Translation: BAE26153.1
BC007188 mRNA Translation: AAH07188.1 Different initiation.
BC012709 mRNA Translation: AAH12709.1
U10435 mRNA Translation: AAA64637.1 Different initiation.
CCDSiCCDS37892.1
RefSeqiNP_001278007.1, NM_001291078.1
NP_035392.1, NM_011262.5
UniGeneiMm.2651

Genome annotation databases

EnsembliENSMUST00000136983; ENSMUSP00000120125; ENSMUSG00000024826
GeneIDi19708
KEGGimmu:19708
UCSCiuc008gfw.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43921 mRNA Translation: AAC52783.1
AF108134 Genomic DNA Translation: AAF21306.1
AK004812 mRNA Translation: BAB23583.1
AK138047 mRNA Translation: BAE23543.1
AK144106 mRNA Translation: BAE25702.1
AK144848 mRNA Translation: BAE26098.1
AK144954 mRNA Translation: BAE26153.1
BC007188 mRNA Translation: AAH07188.1 Different initiation.
BC012709 mRNA Translation: AAH12709.1
U10435 mRNA Translation: AAA64637.1 Different initiation.
CCDSiCCDS37892.1
RefSeqiNP_001278007.1, NM_001291078.1
NP_035392.1, NM_011262.5
UniGeneiMm.2651

3D structure databases

ProteinModelPortaliQ61103
SMRiQ61103
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202862, 10 interactors
ComplexPortaliCPX-1251 Embryonic stem cell-specific SWI/SNF ATP-dependent chromatin remodeling complex
DIPiDIP-59247N
IntActiQ61103, 7 interactors
MINTiQ61103
STRINGi10090.ENSMUSP00000120125

PTM databases

iPTMnetiQ61103
PhosphoSitePlusiQ61103

Proteomic databases

EPDiQ61103
MaxQBiQ61103
PaxDbiQ61103
PeptideAtlasiQ61103
PRIDEiQ61103

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000136983; ENSMUSP00000120125; ENSMUSG00000024826
GeneIDi19708
KEGGimmu:19708
UCSCiuc008gfw.3 mouse

Organism-specific databases

CTDi5977
MGIiMGI:109529 Dpf2

Phylogenomic databases

eggNOGiKOG1244 Eukaryota
ENOG410YDAG LUCA
GeneTreeiENSGT00930000150839
HOVERGENiHBG004475
InParanoidiQ61103
KOiK13196
PhylomeDBiQ61103
TreeFamiTF318971

Miscellaneous databases

ChiTaRSiDpf2 mouse
PROiPR:Q61103
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024826 Expressed in 327 organ(s), highest expression level in ear vesicle
CleanExiMM_DPF2
ExpressionAtlasiQ61103 baseline and differential
GenevisibleiQ61103 MM

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR025750 Requiem/DPF_N_dom
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF00628 PHD, 1 hit
PF14051 Requiem_N, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 2 hits
SM00355 ZnF_C2H2, 1 hit
SUPFAMiSSF57667 SSF57667, 1 hit
SSF57903 SSF57903, 2 hits
PROSITEiView protein in PROSITE
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 2 hits
PS00028 ZINC_FINGER_C2H2_1, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiREQU_MOUSE
AccessioniPrimary (citable) accession number: Q61103
Secondary accession number(s): Q3UNP5, Q60663, Q9QYA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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