UniProtKB - Q61096 (PRTN3_MOUSE)
Protein
Myeloblastin
Gene
Prtn3
Organism
Mus musculus (Mouse)
Status
Functioni
Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. May play a role in neutrophil transendothelial migration, probably when associated with CD177.By similarity
Catalytic activityi
- Hydrolysis of proteins, including elastin, by preferential cleavage: -Ala-|-Xaa- > -Val-|-Xaa-.By similarity EC:3.4.21.76
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 73 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 120 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 205 | Charge relay systemPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- enzyme binding Source: MGI
- serine-type endopeptidase activity Source: MGI
- signaling receptor binding Source: MGI
GO - Biological processi
- cell-cell junction maintenance Source: MGI
- collagen catabolic process Source: UniProtKB-KW
- mature conventional dendritic cell differentiation Source: MGI
- membrane protein ectodomain proteolysis Source: MGI
- negative regulation of phagocytosis Source: MGI
- neutrophil extravasation Source: MGI
- phagocytosis Source: GO_Central
- positive regulation of GTPase activity Source: MGI
- proteolysis Source: MGI
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Collagen degradation |
Enzyme and pathway databases
BRENDAi | 3.4.21.76, 3474 |
Reactomei | R-MMU-140875, Common Pathway of Fibrin Clot Formation R-MMU-6798695, Neutrophil degranulation R-MMU-6803157, Antimicrobial peptides |
Protein family/group databases
MEROPSi | S01.134 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Prtn3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:893580, Prtn3 |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Lysosome
- Lysosome By similarity
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Extracellular side By similarity
Other locations
- Membrane raft By similarity; Peripheral membrane protein By similarity; Extracellular side By similarity
Note: Localizes predominantly to azurophil granules (primary secretory granules) in neutrophils. Secreted upon neutrophil stimulation by TNF-alpha, lipopolysaccharide (LPS), fMLP and CXCL8/IL8 or during neutrophil transmigration. Following secretion tethered to the cell membrane by CD177.By similarity
Cytosol
- cytosol Source: MGI
Extracellular region or secreted
- extracellular space Source: GO_Central
Lysosome
- azurophil granule lumen Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
- plasma membrane raft Source: MGI
Other locations
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
Cell membrane, Lysosome, Membrane, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | By similarityAdd BLAST | 27 | |
PropeptideiPRO_0000027710 | 28 – 29 | By similarity | 2 | |
ChainiPRO_0000027711 | 30 – 250 | MyeloblastinAdd BLAST | 221 | |
PropeptideiPRO_0000027712 | 251 – 254 | By similarity | 4 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 58 ↔ 74 | PROSITE-ProRule annotation | ||
Glycosylationi | 127 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 154 ↔ 211 | PROSITE-ProRule annotation | ||
Glycosylationi | 176 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 184 ↔ 190 | PROSITE-ProRule annotation | ||
Disulfide bondi | 201 ↔ 226 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
CPTACi | non-CPTAC-3494 |
MaxQBi | Q61096 |
PaxDbi | Q61096 |
PeptideAtlasi | Q61096 |
PRIDEi | Q61096 |
ProteomicsDBi | 291826 |
PTM databases
GlyGeni | Q61096, 2 sites |
Interactioni
Subunit structurei
May form dimers.
Interacts with CD177; the interaction tethers PRTN3 to the cell surface; the interaction is direct.
Interacts with SERPINB1.
By similarityGO - Molecular functioni
- enzyme binding Source: MGI
- signaling receptor binding Source: MGI
Protein-protein interaction databases
IntActi | Q61096, 1 interactor |
MINTi | Q61096 |
STRINGi | 10090.ENSMUSP00000006679 |
Chemistry databases
BindingDBi | Q61096 |
Miscellaneous databases
RNActi | Q61096, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 250 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 221 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
InParanoidi | Q61096 |
OrthoDBi | 1314811at2759 |
PhylomeDBi | Q61096 |
TreeFami | TF335284 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q61096-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSGSYPSPKG IHPFLLLALV VGGAVQASKI VGGHEARPHS RPYVASLQLS
60 70 80 90 100
RFPGSHFCGG TLIHPRFVLT AAHCLQDISW QLVTVVLGAH DLLSSEPEQQ
110 120 130 140 150
KFTISQVFQN NYNPEENLND VLLLQLNRTA SLGKEVAVAS LPQQDQTLSQ
160 170 180 190 200
GTQCLAMGWG RLGTQAPTPR VLQELNVTVV TFLCREHNVC TLVPRRAAGI
210 220 230 240 250
CFGDSGGPLI CNGILHGVDS FVIRECASLQ FPDFFARVSM YVDWIQNVLR
GAEP
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4IZY6 | A0A0R4IZY6_MOUSE | Myeloblastin | Prtn3 | 254 | Annotation score: | ||
F6ZK01 | F6ZK01_MOUSE | Myeloblastin | Prtn3 | 126 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | S → A in AAB58055 (PubMed:9187364).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF082186 Genomic DNA Translation: AAC79701.1 U97073 mRNA Translation: AAB58055.1 U43525 mRNA Translation: AAB67271.1 |
CCDSi | CCDS23993.1 |
RefSeqi | NP_035308.2, NM_011178.2 |
Genome annotation databases
GeneIDi | 19152 |
KEGGi | mmu:19152 |
UCSCi | uc007gah.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF082186 Genomic DNA Translation: AAC79701.1 U97073 mRNA Translation: AAB58055.1 U43525 mRNA Translation: AAB67271.1 |
CCDSi | CCDS23993.1 |
RefSeqi | NP_035308.2, NM_011178.2 |
3D structure databases
SMRi | Q61096 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q61096, 1 interactor |
MINTi | Q61096 |
STRINGi | 10090.ENSMUSP00000006679 |
Chemistry databases
BindingDBi | Q61096 |
ChEMBLi | CHEMBL5384 |
Protein family/group databases
MEROPSi | S01.134 |
PTM databases
GlyGeni | Q61096, 2 sites |
Proteomic databases
CPTACi | non-CPTAC-3494 |
MaxQBi | Q61096 |
PaxDbi | Q61096 |
PeptideAtlasi | Q61096 |
PRIDEi | Q61096 |
ProteomicsDBi | 291826 |
Protocols and materials databases
DNASUi | 19152 |
Genome annotation databases
GeneIDi | 19152 |
KEGGi | mmu:19152 |
UCSCi | uc007gah.1, mouse |
Organism-specific databases
CTDi | 5657 |
MGIi | MGI:893580, Prtn3 |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
InParanoidi | Q61096 |
OrthoDBi | 1314811at2759 |
PhylomeDBi | Q61096 |
TreeFami | TF335284 |
Enzyme and pathway databases
BRENDAi | 3.4.21.76, 3474 |
Reactomei | R-MMU-140875, Common Pathway of Fibrin Clot Formation R-MMU-6798695, Neutrophil degranulation R-MMU-6803157, Antimicrobial peptides |
Miscellaneous databases
BioGRID-ORCSi | 19152, 1 hit in 52 CRISPR screens |
ChiTaRSi | Prtn3, mouse |
PROi | PR:Q61096 |
RNActi | Q61096, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRTN3_MOUSE | |
Accessioni | Q61096Primary (citable) accession number: Q61096 Secondary accession number(s): O08809 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 30, 2002 |
Last sequence update: | August 1, 1999 | |
Last modified: | April 7, 2021 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families