Prtn3

Functionⁱ

Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. May play a role in neutrophil transendothelial migration, probably when associated with CD177.By similarity

Catalytic activityⁱ

Hydrolysis of proteins, including elastin, by preferential cleavage: -Ala-|-Xaa- > -Val-|-Xaa-.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	73	Charge relay systemPROSITE-ProRule annotation	1
Active siteⁱ	120	Charge relay systemPROSITE-ProRule annotation	1
Active siteⁱ	205	Charge relay systemPROSITE-ProRule annotation	1

GO - Molecular functionⁱ

enzyme binding Source: MGI
serine-type endopeptidase activity Source: MGI
signaling receptor binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell-cell junction maintenance Source: MGI
collagen catabolic process Source: UniProtKB-KW
mature conventional dendritic cell differentiation Source: MGI
membrane protein ectodomain proteolysis Source: MGI
negative regulation of phagocytosis Source: MGI
neutrophil extravasation Source: MGI
phagocytosis Source: GO_Central
positive regulation of GTPase activity Source: MGI
proteolysis Source: MGI

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Protease, Serine protease
Biological process	Collagen degradation

Enzyme and pathway databases

BRENDAⁱ	3.4.21.76 3474

BRENDAⁱ

3.4.21.76 3474

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	S01.134

MEROPSⁱ

S01.134

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Myeloblastin (EC:3.4.21.76) Alternative name(s): Proteinase 3 Short name: PR-3
Gene namesⁱ	Name:Prtn3
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Unplaced

Organism-specific databases

MGIⁱ	MGI:893580 Prtn3

Keywords - Cellular componentⁱ

Cell membrane, Lysosome, Membrane, Secreted

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL5384

ChEMBLⁱ

CHEMBL5384

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Signal peptideⁱ	1 – 27	By similarityAdd BLAST	27
PropeptideⁱPRO_0000027710	28 – 29	By similarity	2
ChainⁱPRO_0000027711	30 – 250	MyeloblastinAdd BLAST	221
PropeptideⁱPRO_0000027712	251 – 254	By similarity	4

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	58 ↔ 74	PROSITE-ProRule annotation
Glycosylationⁱ	127	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	154 ↔ 211	PROSITE-ProRule annotation
Glycosylationⁱ	176	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	184 ↔ 190	PROSITE-ProRule annotation
Disulfide bondⁱ	201 ↔ 226	PROSITE-ProRule annotation

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q61096
PaxDbⁱ	Q61096
PeptideAtlas More...PeptideAtlasⁱ	Q61096
PRIDEⁱ	Q61096

Expressionⁱ

Gene expression databases

CleanExⁱ	MM_PRTN3

CleanExⁱ

MM_PRTN3

Interactionⁱ

Subunit structureⁱ

May form dimers. Interacts with CD177; the interaction tethers PRTN3 to the cell surface; the interaction is direct.By similarity

GO - Molecular functionⁱ

enzyme binding Source: MGI
signaling receptor binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

IntActⁱ	Q61096, 1 interactor
Molecular INTeraction database More...MINTⁱ	Q61096
STRINGⁱ	10090.ENSMUSP00000006679

Chemistry databases

BindingDBⁱ

Q61096

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q61096
SMRⁱ	Q61096
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	30 – 250	Peptidase S1PROSITE-ProRule annotationAdd BLAST		221

Sequence similaritiesⁱ

Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	KOG3627 Eukaryota COG5640 LUCA
HOGENOMⁱ	HOG000251820
HOVERGENⁱ	HBG013304
InParanoidⁱ	Q61096
KEGG Orthology (KO) More...KOⁱ	K01350
PhylomeDBⁱ	Q61096
TreeFamⁱ	TF335284

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00190 Tryp_SPc, 1 hit
InterProⁱ	View protein in InterPro IPR009003 Peptidase_S1_PA IPR001314 Peptidase_S1A IPR001254 Trypsin_dom IPR018114 TRYPSIN_HIS IPR033116 TRYPSIN_SER
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00089 Trypsin, 1 hit
PRINTSⁱ	PR00722 CHYMOTRYPSIN
SMARTⁱ	View protein in SMART SM00020 Tryp_SPc, 1 hit
SUPFAMⁱ	SSF50494 SSF50494, 1 hit
PROSITEⁱ	View protein in PROSITE PS50240 TRYPSIN_DOM, 1 hit PS00134 TRYPSIN_HIS, 1 hit PS00135 TRYPSIN_SER, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q61096-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSGSYPSPKG IHPFLLLALV VGGAVQASKI VGGHEARPHS RPYVASLQLS 
        60         70         80         90        100
RFPGSHFCGG TLIHPRFVLT AAHCLQDISW QLVTVVLGAH DLLSSEPEQQ 
       110        120        130        140        150
KFTISQVFQN NYNPEENLND VLLLQLNRTA SLGKEVAVAS LPQQDQTLSQ 
       160        170        180        190        200
GTQCLAMGWG RLGTQAPTPR VLQELNVTVV TFLCREHNVC TLVPRRAAGI 
       210        220        230        240        250
CFGDSGGPLI CNGILHGVDS FVIRECASLQ FPDFFARVSM YVDWIQNVLR 

GAEP

Length:254

Mass (Da):27,626

Last modified:August 1, 1999 - v2

Checksum:ⁱ00CEB989A3CB79CA

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	2	S → A in AAB58055 (PubMed:9187364).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF082186 Genomic DNA Translation: AAC79701.1 U97073 mRNA Translation: AAB58055.1 U43525 mRNA Translation: AAB67271.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS23993.1
NCBI Reference Sequences More...RefSeqⁱ	NP_035308.2, NM_011178.2
UniGeneⁱ	Mm.2364

Genome annotation databases

GeneIDⁱ	19152
KEGGⁱ	mmu:19152
UCSC genome browser More...UCSCⁱ	uc007gah.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
Q61096	Proteinase 3		MOUSE		98
Proteinase 3		RAT		256
UPI000A3122FA		MUSPA		254
UPI000A31AE49		MUSCR		254
Proteinase 3		RAT		254
+1

Protein	Similar proteins		Species	Score	Length
Q61096	Myeloblastin	)	HUMAN		256
Proteinase 3		GORGO		256
Proteinase 3		PANTR		256
Uncharacterized protein		PANPA		133
Proteinase 3		MOUSE		98
+76

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF082186 Genomic DNA Translation: AAC79701.1 U97073 mRNA Translation: AAB58055.1 U43525 mRNA Translation: AAB67271.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS23993.1
NCBI Reference Sequences More...RefSeqⁱ	NP_035308.2, NM_011178.2
UniGeneⁱ	Mm.2364

3D structure databases

ProteinModelPortalⁱ	Q61096
SMRⁱ	Q61096
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	Q61096, 1 interactor
Molecular INTeraction database More...MINTⁱ	Q61096
STRINGⁱ	10090.ENSMUSP00000006679

Chemistry databases

BindingDBⁱ	Q61096
ChEMBLⁱ	CHEMBL5384

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	S01.134

MEROPSⁱ

S01.134

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q61096
PaxDbⁱ	Q61096
PeptideAtlas More...PeptideAtlasⁱ	Q61096
PRIDEⁱ	Q61096

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	19152
Structural Biology Knowledgebase	Search...

Genome annotation databases

GeneIDⁱ	19152
KEGGⁱ	mmu:19152
UCSC genome browser More...UCSCⁱ	uc007gah.1 mouse

Organism-specific databases

CTDⁱ	5657
MGIⁱ	MGI:893580 Prtn3

Phylogenomic databases

eggNOGⁱ	KOG3627 Eukaryota COG5640 LUCA
HOGENOMⁱ	HOG000251820
HOVERGENⁱ	HBG013304
InParanoidⁱ	Q61096
KEGG Orthology (KO) More...KOⁱ	K01350
PhylomeDBⁱ	Q61096
TreeFamⁱ	TF335284

Enzyme and pathway databases

BRENDAⁱ	3.4.21.76 3474

BRENDAⁱ

3.4.21.76 3474

Miscellaneous databases

ChiTaRSⁱ	Prtn3 mouse
Protein Ontology More...PROⁱ	PR:Q61096
SOURCEⁱ	Search...

Gene expression databases

CleanExⁱ	MM_PRTN3

CleanExⁱ

MM_PRTN3

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00190 Tryp_SPc, 1 hit
InterProⁱ	View protein in InterPro IPR009003 Peptidase_S1_PA IPR001314 Peptidase_S1A IPR001254 Trypsin_dom IPR018114 TRYPSIN_HIS IPR033116 TRYPSIN_SER
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00089 Trypsin, 1 hit
PRINTSⁱ	PR00722 CHYMOTRYPSIN
SMARTⁱ	View protein in SMART SM00020 Tryp_SPc, 1 hit
SUPFAMⁱ	SSF50494 SSF50494, 1 hit
PROSITEⁱ	View protein in PROSITE PS50240 TRYPSIN_DOM, 1 hit PS00134 TRYPSIN_HIS, 1 hit PS00135 TRYPSIN_SER, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PRTN3_MOUSE
Accessionⁱ	Q61096Primary (citable) accession number: Q61096 Secondary accession number(s): O08809
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
	Last sequence update:	August 1, 1999
	Last modified:	May 23, 2018
	This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
Peptidase families
Classification of peptidase families and list of entries
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q61096 (PRTN3_MOUSE)

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Myeloblastin

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Lysosome

Extracellular region or secreted

Plasma membrane

Other locations

Cytosol

Extracellular region or secreted

Lysosome

Plasma Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ