UniProtKB - Q61093 (CY24B_MOUSE)
Cytochrome b-245 heavy chain
Cybb
Functioni
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 101 | Iron (heme axial ligand)Curated | 1 | |
Metal bindingi | 115 | Iron (heme axial ligand)Curated | 1 | |
Metal bindingi | 209 | Iron (heme axial ligand)Curated | 1 | |
Metal bindingi | 222 | Iron (heme axial ligand)Curated | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 338 – 344 | FADSequence analysis | 7 |
GO - Molecular functioni
- electron transfer activity Source: Ensembl
- flavin adenine dinucleotide binding Source: MGI
- heme binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- protein heterodimerization activity Source: MGI
- superoxide-generating NAD(P)H oxidase activity Source: MGI
- voltage-gated ion channel activity Source: UniProtKB-KW
GO - Biological processi
- cellular response to cadmium ion Source: Ensembl
- cellular response to ethanol Source: Ensembl
- cellular response to L-glutamine Source: Ensembl
- defense response Source: GO_Central
- hydrogen peroxide biosynthetic process Source: MGI
- hypoxia-inducible factor-1alpha signaling pathway Source: MGI
- inflammatory response Source: UniProtKB
- innate immune response Source: UniProtKB
- oxidation-reduction process Source: MGI
- positive regulation of angiogenesis Source: MGI
- positive regulation of tumor necrosis factor production Source: MGI
- regulation of ion transmembrane transport Source: UniProtKB-KW
- respiratory burst Source: MGI
- response to aldosterone Source: Ensembl
- response to angiotensin Source: Ensembl
- response to drug Source: MGI
- response to nutrient Source: Ensembl
- superoxide anion generation Source: MGI
- superoxide metabolic process Source: UniProtKB
Keywordsi
Molecular function | Ion channel, Oxidoreductase, Voltage-gated channel |
Biological process | Electron transport, Ion transport, Transport |
Ligand | FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP |
Enzyme and pathway databases
Reactomei | R-MMU-1222556, ROS and RNS production in phagocytes R-MMU-1236973, Cross-presentation of particulate exogenous antigens (phagosomes) R-MMU-3299685, Detoxification of Reactive Oxygen Species R-MMU-4420097, VEGFA-VEGFR2 Pathway R-MMU-5668599, RHO GTPases Activate NADPH Oxidases R-MMU-6798695, Neutrophil degranulation |
Protein family/group databases
PeroxiBasei | 5957, MmNOx02 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome b-245 heavy chain (EC:1.-.-.-)Alternative name(s): CGD91-phox Cytochrome b(558) subunit beta Short name: Cytochrome b558 subunit beta Heme-binding membrane glycoprotein gp91phox Neutrophil cytochrome b 91 kDa polypeptide gp91-1 gp91-phox p22 phagocyte B-cytochrome |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88574, Cybb |
Subcellular locationi
Plasma membrane
Note: As unassembled monomer may localize to the endoplasmic reticulum.1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- perinuclear endoplasmic reticulum Source: Ensembl
- rough endoplasmic reticulum Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Nucleus
- nuclear envelope Source: MGI
Plasma Membrane
- integral component of plasma membrane Source: MGI
- NADPH oxidase complex Source: UniProtKB
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: ParkinsonsUK-UCL
- dendrite Source: MGI
- neuronal cell body Source: MGI
- phagocytic vesicle Source: ParkinsonsUK-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 9 – 29 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 30 – 48 | ExtracellularSequence analysisAdd BLAST | 19 | |
Transmembranei | 49 – 69 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 70 – 102 | CytoplasmicSequence analysisAdd BLAST | 33 | |
Transmembranei | 103 – 123 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 124 – 169 | ExtracellularSequence analysisAdd BLAST | 46 | |
Transmembranei | 170 – 190 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 191 – 200 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 201 – 221 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 222 – 261 | ExtracellularSequence analysisAdd BLAST | 40 | |
Transmembranei | 262 – 282 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 283 – 570 | CytoplasmicSequence analysisAdd BLAST | 288 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000210146 | 1 – 570 | Cytochrome b-245 heavy chainAdd BLAST | 570 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 40 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Cross-linki | 159 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 161 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 255 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 294 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 299 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 306 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 328 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 334 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 381 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 506 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 567 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q61093 |
MaxQBi | Q61093 |
PaxDbi | Q61093 |
PRIDEi | Q61093 |
PTM databases
GlyGeni | Q61093, 1 site |
iPTMneti | Q61093 |
PhosphoSitePlusi | Q61093 |
SwissPalmi | Q61093 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000015340, Expressed in mesenteric lymph node and 158 other tissues |
ExpressionAtlasi | Q61093, baseline and differential |
Genevisiblei | Q61093, MM |
Interactioni
Subunit structurei
Composed of a heavy chain (beta) and a light chain (alpha).
Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4.
Interacts with NCF1.
Interacts with calprotectin (S100A8/9) (By similarity).
Interacts with NRROS; the interaction is direct and impairs formation of a stable NADPH oxidase complex (PubMed:24739962).
Interacts with CYBC1; CYBC1 may act as a chaperone stabilizing Cytochrome b-245 heterodimer (By similarity).
By similarity1 PublicationBinary interactionsi
Hide detailsGO - Molecular functioni
- protein heterodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 198990, 29 interactors |
DIPi | DIP-60841N |
IntActi | Q61093, 5 interactors |
STRINGi | 10090.ENSMUSP00000015484 |
Miscellaneous databases
RNActi | Q61093, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 54 – 286 | Ferric oxidoreductaseAdd BLAST | 233 | |
Domaini | 287 – 397 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 111 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0039, Eukaryota |
GeneTreei | ENSGT00940000160244 |
HOGENOMi | CLU_005646_3_1_1 |
InParanoidi | Q61093 |
OMAi | GHVMLLC |
OrthoDBi | 936110at2759 |
PhylomeDBi | Q61093 |
TreeFami | TF105354 |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR000778, Cyt_b245_heavy_chain IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit |
PRINTSi | PR00466, GP91PHOX |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGNWAVNEGL SIFVILVWLG LNVFLFINYY KVYDDGPKYN YTRKLLGSAL
60 70 80 90 100
ALARAPAACL NFNCMLILLP VCRNLLSFLR GSSACCSTRI RRQLDRNLTF
110 120 130 140 150
HKMVAWMIAL HTAIHTIAHL FNVEWCVNAR VGISDRYSIA LSDIGDNENE
160 170 180 190 200
EYLNFAREKI KNPEGGLYVA VTRLAGITGI VITLCLILII TSSTKTIRRS
210 220 230 240 250
YFEVFWYTHH LFVIFFIGLA IHGAERIVRG QTAESLEEHN LDICADKIEE
260 270 280 290 300
WGKIKECPVP KFAGNPPMTW KWIVGPMFLY LCERLVRFWR SQQKVVITKV
310 320 330 340 350
VTHPFKTIEL QMKKKGFKME VGQYIFVKCP KVSKLEWHPF TLTSAPEEDF
360 370 380 390 400
FSIHIRIVGD WTEGLFNACG CDKQEFQDAW KLPKIAVDGP FGTASEDVFS
410 420 430 440 450
YEVVMLVGAG IGVTPFASIL KSVWYKYCDN ATSLKLKKIY FYWLCRDTHA
460 470 480 490 500
FEWFADLLQL LETQMQERNN ANFLSYNIYL TGWDESQANH FAVHHDEEKD
510 520 530 540 550
VITGLKQKTL YGRPNWDNEF KTIASEHPNT TIGVFLCGPE ALAETLSKQS
560 570
ISNSESGPRG VHFIFNKENF
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q7S3 | E9Q7S3_MOUSE | Cytochrome b-245 heavy chain | Cybb | 93 | Annotation score: | ||
E9Q802 | E9Q802_MOUSE | Cytochrome b-245 heavy chain | Cybb | 114 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U43384 mRNA Translation: AAB05997.1 BC071229 mRNA Translation: AAH71229.1 |
CCDSi | CCDS30010.1 |
RefSeqi | NP_031833.3, NM_007807.5 XP_006527628.1, XM_006527565.3 |
Genome annotation databases
Ensembli | ENSMUST00000015484; ENSMUSP00000015484; ENSMUSG00000015340 |
GeneIDi | 13058 |
KEGGi | mmu:13058 |
UCSCi | uc009spv.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U43384 mRNA Translation: AAB05997.1 BC071229 mRNA Translation: AAH71229.1 |
CCDSi | CCDS30010.1 |
RefSeqi | NP_031833.3, NM_007807.5 XP_006527628.1, XM_006527565.3 |
3D structure databases
SMRi | Q61093 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198990, 29 interactors |
DIPi | DIP-60841N |
IntActi | Q61093, 5 interactors |
STRINGi | 10090.ENSMUSP00000015484 |
Protein family/group databases
PeroxiBasei | 5957, MmNOx02 |
PTM databases
GlyGeni | Q61093, 1 site |
iPTMneti | Q61093 |
PhosphoSitePlusi | Q61093 |
SwissPalmi | Q61093 |
Proteomic databases
jPOSTi | Q61093 |
MaxQBi | Q61093 |
PaxDbi | Q61093 |
PRIDEi | Q61093 |
Protocols and materials databases
Antibodypediai | 24864, 484 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000015484; ENSMUSP00000015484; ENSMUSG00000015340 |
GeneIDi | 13058 |
KEGGi | mmu:13058 |
UCSCi | uc009spv.3, mouse |
Organism-specific databases
CTDi | 1536 |
MGIi | MGI:88574, Cybb |
Phylogenomic databases
eggNOGi | KOG0039, Eukaryota |
GeneTreei | ENSGT00940000160244 |
HOGENOMi | CLU_005646_3_1_1 |
InParanoidi | Q61093 |
OMAi | GHVMLLC |
OrthoDBi | 936110at2759 |
PhylomeDBi | Q61093 |
TreeFami | TF105354 |
Enzyme and pathway databases
Reactomei | R-MMU-1222556, ROS and RNS production in phagocytes R-MMU-1236973, Cross-presentation of particulate exogenous antigens (phagosomes) R-MMU-3299685, Detoxification of Reactive Oxygen Species R-MMU-4420097, VEGFA-VEGFR2 Pathway R-MMU-5668599, RHO GTPases Activate NADPH Oxidases R-MMU-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 13058, 0 hits in 17 CRISPR screens |
ChiTaRSi | Cybb, mouse |
PROi | PR:Q61093 |
RNActi | Q61093, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000015340, Expressed in mesenteric lymph node and 158 other tissues |
ExpressionAtlasi | Q61093, baseline and differential |
Genevisiblei | Q61093, MM |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR000778, Cyt_b245_heavy_chain IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit |
PRINTSi | PR00466, GP91PHOX |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CY24B_MOUSE | |
Accessioni | Q61093Primary (citable) accession number: Q61093 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot