Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Etoposide-induced protein 2.4

Gene

Ei24

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a negative growth regulator via p53-mediated apoptosis pathway. Regulates formation of degradative autolysosomes during autophagy (By similarity).By similarity2 Publications

GO - Biological processi

  • autophagy Source: MGI
  • cellular response to UV-C Source: MGI
  • intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  • macroautophagy Source: GO_Central
  • negative regulation of cell growth Source: MGI
  • negative regulation of protein import into nucleus Source: MGI
  • neuromuscular process controlling balance Source: MGI
  • positive regulation of intrinsic apoptotic signaling pathway Source: MGI
  • response to drug Source: MGI

Keywordsi

Biological processApoptosis, Autophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Etoposide-induced protein 2.4
Alternative name(s):
p53-induced gene 8 protein
Gene namesi
Name:Ei24
Synonyms:Pig8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:108090 Ei24

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei77 – 97HelicalSequence analysisAdd BLAST21
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Transmembranei179 – 199HelicalSequence analysisAdd BLAST21
Transmembranei238 – 255HelicalSequence analysisAdd BLAST18
Transmembranei257 – 277HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000869462 – 340Etoposide-induced protein 2.4Add BLAST339

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei46PhosphoserineBy similarity1
Modified residuei47PhosphoserineBy similarity1
Modified residuei56PhosphoserineCombined sources1
Modified residuei320PhosphoserineCombined sources1
Modified residuei326PhosphoserineCombined sources1
Modified residuei330PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ61070
MaxQBiQ61070
PaxDbiQ61070
PeptideAtlasiQ61070
PRIDEiQ61070

PTM databases

iPTMnetiQ61070
PhosphoSitePlusiQ61070

Expressioni

Tissue specificityi

Found in all the examined tissues. High expression was found in liver, skeletal muscle, pancreas, kidney heart and to a lesser extent in brain, placenta and lung.

Inductioni

By etoposide treatment, induction requires p53. Etoposide induces DNA damage in cells by inhibiting DNA topoisomerase II, and ultimately causes apoptotic cell death.2 Publications

Gene expression databases

CleanExiMM_EI24

Interactioni

Subunit structurei

Interacts with BCL2.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ61070
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni52 – 115Interaction with BH3 domain of BCL2Add BLAST64

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 44Poly-Arg4
Compositional biasi316 – 320Poly-Ser5

Sequence similaritiesi

Belongs to the EI24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3966 Eukaryota
ENOG410XRPU LUCA
HOGENOMiHOG000293197
HOVERGENiHBG001857
InParanoidiQ61070
KOiK10134
PhylomeDBiQ61070
TreeFamiTF314441

Family and domain databases

InterProiView protein in InterPro
IPR009890 EI24
PANTHERiPTHR21389 PTHR21389, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q61070-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADSVKTFLQ DLGRGIKDSI WGICTISKLD ARIQQKREEQ RRRRASSLLA
60 70 80 90 100
QRRPQSVERK QESEPRIVSR IFQCCAWNGG VFWFSLLLFY RVFIPVLQSV
110 120 130 140 150
TARIIGDPSL HGDVWSWLEF FLTSIFSALW VLPLFVLSKV VNAIWFQDIA
160 170 180 190 200
DLAFEVSGRK PHPFPSVSKI IADMLFNLLL QALFLIQGMF VSLFPIHLVG
210 220 230 240 250
QLVSLLHMSL LYSLYCFEYR WFNKGIEMHQ RLSNIERNWP YYFGFGLPLA
260 270 280 290 300
FLTAMQSSYI ISGCLFSILF PLFIISANEA KTPGKAYLFQ LRLFSLVVFL
310 320 330 340
SNRLFHKTVY LQSALSSSSS AEKFPSPHPS PAKLKAAAGH
Length:340
Mass (Da):38,933
Last modified:May 5, 2009 - v3
Checksum:iAA3B824B2CE330BC
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J250A0A0R4J250_MOUSE
Etoposide induced 2.4 mRNA
Ei24 mCG_4131
358Annotation score:
V9GXH2V9GXH2_MOUSE
Etoposide-induced protein 2.4
Ei24
181Annotation score:
V9GXU0V9GXU0_MOUSE
Etoposide-induced protein 2.4
Ei24
171Annotation score:

Sequence cautioni

The sequence AAC52483 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAE28130 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAE35489 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAE42899 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78N → D in BAE28130 (PubMed:16141072).Curated1
Sequence conflicti144I → F in BAE35489 (PubMed:16141072).Curated1
Sequence conflicti320S → P in BAE42899 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41751 mRNA Translation: AAC52483.2 Different initiation.
AK147776 mRNA Translation: BAE28130.1 Different initiation.
AK159927 mRNA Translation: BAE35489.1 Different initiation.
AK172233 mRNA Translation: BAE42899.1 Different initiation.
RefSeqiNP_001186423.1, NM_001199494.1
NP_031941.1, NM_007915.5
UniGeneiMm.4337

Genome annotation databases

GeneIDi13663
KEGGimmu:13663
UCSCiuc009oua.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41751 mRNA Translation: AAC52483.2 Different initiation.
AK147776 mRNA Translation: BAE28130.1 Different initiation.
AK159927 mRNA Translation: BAE35489.1 Different initiation.
AK172233 mRNA Translation: BAE42899.1 Different initiation.
RefSeqiNP_001186423.1, NM_001199494.1
NP_031941.1, NM_007915.5
UniGeneiMm.4337

3D structure databases

ProteinModelPortaliQ61070
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ61070
PhosphoSitePlusiQ61070

Proteomic databases

EPDiQ61070
MaxQBiQ61070
PaxDbiQ61070
PeptideAtlasiQ61070
PRIDEiQ61070

Protocols and materials databases

DNASUi13663
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13663
KEGGimmu:13663
UCSCiuc009oua.2 mouse

Organism-specific databases

CTDi9538
MGIiMGI:108090 Ei24

Phylogenomic databases

eggNOGiKOG3966 Eukaryota
ENOG410XRPU LUCA
HOGENOMiHOG000293197
HOVERGENiHBG001857
InParanoidiQ61070
KOiK10134
PhylomeDBiQ61070
TreeFamiTF314441

Miscellaneous databases

ChiTaRSiEi24 mouse
PROiPR:Q61070
SOURCEiSearch...

Gene expression databases

CleanExiMM_EI24

Family and domain databases

InterProiView protein in InterPro
IPR009890 EI24
PANTHERiPTHR21389 PTHR21389, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEI24_MOUSE
AccessioniPrimary (citable) accession number: Q61070
Secondary accession number(s): Q3T9X1, Q3TVX9, Q3UGS7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 5, 2009
Last modified: February 28, 2018
This is version 121 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again