UniProtKB - Q61069 (USF1_MOUSE)

BLAST

>sp|Q61069|USF1_MOUSE Upstream stimulatory factor 1 OS=Mus musculus OX=10090 GN=Usf1 PE=1 SV=1
MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVM
YRVIQVSEGQLDGQTEGSGAISGYPATQSMTQAVIQGAFTSDDAVDTEGAAAETHYTYFP
STAVGDGSGGTTSGSTTAVVTTQGSEALLGQATPPSTGQFFVMMSPQEVLQGGSQRSIAP
RTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSK
GGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHG
LEVVIKNDSN

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History
Entry version 133 (07 Nov 2018)
Sequence version 1 (01 Nov 1996)
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Protein

Upstream stimulatory factor 1

Gene

Usf1

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

GO - Molecular functionⁱ

bHLH transcription factor binding Source: MGI
DNA binding
Source: MGI
Inferred from direct assayⁱ
- 15466854
DNA-binding transcription factor activity
Source: MGI
Inferred from direct assayⁱ
- 15466854
double-stranded DNA binding Source: MGI
enzyme binding Source: MGI
histone deacetylase binding Source: MGI
identical protein binding Source: MGI
protein heterodimerization activity Source: MGI
protein homodimerization activity Source: MGI
protein kinase binding Source: MGI
sequence-specific DNA binding Source: MGI
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular response to insulin stimulus Source: MGI
glucose metabolic process Source: MGI
late viral transcription Source: MGI
lipid homeostasis
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 9890958
positive regulation of transcription, DNA-templated
Source: MGI
Inferred from direct assayⁱ
- 15466854
positive regulation of transcription by RNA polymerase II Source: MGI
positive regulation of transcription from RNA polymerase II promoter by glucose
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 9890958
regulation of transcription, DNA-templated Source: MGI
response to hypoxia Source: MGI
response to UV
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 15358786
transcription by RNA polymerase II Source: MGI

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

Reactomeⁱ	R-MMU-9018519 Estrogen-dependent gene expression

Reactomeⁱ

R-MMU-9018519 Estrogen-dependent gene expression

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Upstream stimulatory factor 1 Alternative name(s): Major late transcription factor 1
Gene namesⁱ	Name:Usf1 Synonyms:Usf
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 1

Organism-specific databases

MGIⁱ	MGI:99542 Usf1

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127497	1 – 310	Upstream stimulatory factor 1Add BLAST		310

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Cross-linkⁱ	306	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMⁱ

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDⁱ	Q61069
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q61069
PaxDbⁱ	Q61069
PRIDEⁱ	Q61069

PTM databases

iPTMnetⁱ	Q61069
PhosphoSitePlusⁱ	Q61069

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000026641 Expressed in 102 organ(s), highest expression level in spleen
CleanExⁱ	MM_USF1
ExpressionAtlasⁱ	Q61069 baseline and differential
Genevisibleⁱ	Q61069 MM

Interactionⁱ

Subunit structureⁱ

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2).

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Stat1	P42225	2	EBI-2325635,EBI-647118

With

Entry

#Exp.

IntAct

Notes

Stat1

P42225

EBI-2325635,EBI-647118

GO - Molecular functionⁱ

bHLH transcription factor binding Source: MGI
enzyme binding Source: MGI
histone deacetylase binding Source: MGI
identical protein binding Source: MGI
protein heterodimerization activity Source: MGI
protein homodimerization activity Source: MGI
protein kinase binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	204464, 5 interactors
ComplexPortalⁱ	CPX-3085 USF1 upstream stimulatory factor complex CPX-3086 USF1-USF2 upstream stimulatory factor complex
IntActⁱ	Q61069, 1 interactor
STRINGⁱ	10090.ENSMUSP00000125729

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q61069
SMRⁱ	Q61069
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	199 – 254	bHLHPROSITE-ProRule annotationAdd BLAST		56

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	271 – 292	Leucine-zipperAdd BLAST		22

Phylogenomic databases

eggNOGⁱ	ENOG410INSE Eukaryota ENOG4110B3H LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000004498
HOVERGENⁱ	HBG004346
InParanoidⁱ	Q61069
KEGG Orthology (KO) More...KOⁱ	K09106
OMAⁱ	NQRTIAP
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0RH5
PhylomeDBⁱ	Q61069
TreeFamⁱ	TF323338

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR024097 bHLH_ZIP_TF IPR036638 HLH_DNA-bd_sf
PANTHERⁱ	PTHR12565 PTHR12565, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show all Align All

Q61069-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKGQQKTAET EEGTVQIQEG AVATGEDPTS VAIASIQSAA TFPDPNVKYV 
        60         70         80         90        100
FRTENGGQVM YRVIQVSEGQ LDGQTEGSGA ISGYPATQSM TQAVIQGAFT 
       110        120        130        140        150
SDDAVDTEGA AAETHYTYFP STAVGDGSGG TTSGSTTAVV TTQGSEALLG 
       160        170        180        190        200
QATPPSTGQF FVMMSPQEVL QGGSQRSIAP RTHPYSPKSE APRTTRDEKR 
       210        220        230        240        250
RAQHNEVERR RRDKINNWIV QLSKIIPDCS MESTKSGQSK GGILSKACDY 
       260        270        280        290        300
IQELRQSNHR LSEELQGLDQ LQLDNDVLRQ QVEDLKNKNL LLRAQLRHHG 
       310
LEVVIKNDSN

Length:310

Mass (Da):33,570

Last modified:November 1, 1996 - v1

Checksum:ⁱ7D0AF808A2FDFA1C

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

E9Q722	E9Q722_MOUSE	Upstream stimulatory factor 1 Upstream stimulatory factor 1	Usf1	296	Annotation score:
E0CYP7	E0CYP7_MOUSE	Upstream stimulatory factor 1 Upstream stimulatory factor 1	Usf1	148	Annotation score:
F8WGH6	F8WGH6_MOUSE	Upstream stimulatory factor 1 Upstream stimulatory factor 1	Usf1	48	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U41741 Genomic DNA Translation: AAC52921.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS15495.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001292605.1, NM_001305676.1 NP_001292606.1, NM_001305677.1 NP_033506.1, NM_009480.3
UniGeneⁱ	Mm.8

Genome annotation databases

Ensemblⁱ	ENSMUST00000161241; ENSMUSP00000125729; ENSMUSG00000026641 ENSMUST00000167546; ENSMUSP00000128913; ENSMUSG00000026641
GeneIDⁱ	22278
KEGGⁱ	mmu:22278
UCSC genome browser More...UCSCⁱ	uc007doj.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q61069	Upstream transcription factor 1, isoform CRA_a		MOUSE		310	UniRef100_Q61069
Upstream transcription factor 1 short form		MOUSE		251
Upstream stimulatory factor 1 (Fragment)		MOUSE		148

Protein	Similar proteins		Species	Score	Length	Source
Q61069	Upstream stimulatory factor 1	)	HUMAN		310	UniRef90_P22415
Upstream stimulatory factor 1		RABIT		310
Upstream stimulatory factor 1		BOVIN		310
Upstream stimulatory factor 1 isoform 1		CALJA		310
USF1 isoform 1		PANTR		310
+158

Protein	Similar proteins		Species	Score	Length	Source
Q61069	Upstream stimulatory factor 1	)	HUMAN		310	UniRef50_P22415
Upstream stimulatory factor 1		BOVIN		310
Upstream stimulatory factor 1		RABIT		310
Upstream stimulatory factor 1 isoform 1		CALJA		310
USF1 isoform 1		PANTR		310
+306

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U41741 Genomic DNA Translation: AAC52921.1
CCDSⁱ	CCDS15495.1
RefSeqⁱ	NP_001292605.1, NM_001305676.1 NP_001292606.1, NM_001305677.1 NP_033506.1, NM_009480.3
UniGeneⁱ	Mm.8

3D structure databases

ProteinModelPortalⁱ	Q61069
SMRⁱ	Q61069
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	204464, 5 interactors
ComplexPortalⁱ	CPX-3085 USF1 upstream stimulatory factor complex CPX-3086 USF1-USF2 upstream stimulatory factor complex
IntActⁱ	Q61069, 1 interactor
STRINGⁱ	10090.ENSMUSP00000125729

PTM databases

iPTMnetⁱ	Q61069
PhosphoSitePlusⁱ	Q61069

Proteomic databases

EPDⁱ	Q61069
MaxQBⁱ	Q61069
PaxDbⁱ	Q61069
PRIDEⁱ	Q61069

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000161241; ENSMUSP00000125729; ENSMUSG00000026641 ENSMUST00000167546; ENSMUSP00000128913; ENSMUSG00000026641
GeneIDⁱ	22278
KEGGⁱ	mmu:22278
UCSCⁱ	uc007doj.2 mouse

Organism-specific databases

CTDⁱ	7391
MGIⁱ	MGI:99542 Usf1

Phylogenomic databases

eggNOGⁱ	ENOG410INSE Eukaryota ENOG4110B3H LUCA
GeneTreeⁱ	ENSGT00390000004498
HOVERGENⁱ	HBG004346
InParanoidⁱ	Q61069
KOⁱ	K09106
OMAⁱ	NQRTIAP
OrthoDBⁱ	EOG091G0RH5
PhylomeDBⁱ	Q61069
TreeFamⁱ	TF323338

Enzyme and pathway databases

Reactomeⁱ	R-MMU-9018519 Estrogen-dependent gene expression

Reactomeⁱ

R-MMU-9018519 Estrogen-dependent gene expression

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q61069
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000026641 Expressed in 102 organ(s), highest expression level in spleen
CleanExⁱ	MM_USF1
ExpressionAtlasⁱ	Q61069 baseline and differential
Genevisibleⁱ	Q61069 MM

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR024097 bHLH_ZIP_TF IPR036638 HLH_DNA-bd_sf
PANTHERⁱ	PTHR12565 PTHR12565, 1 hit
Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	USF1_MOUSE
Accessionⁱ	Q61069Primary (citable) accession number: Q61069
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	November 1, 1996
	Last modified:	November 7, 2018
	This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q61069 (USF1_MOUSE)

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Upstream stimulatory factor 1

Usf1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ