We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - Q61069 (USF1_MOUSE)
Protein
Upstream stimulatory factor 1
Gene
Usf1
Organism
Mus musculus (Mouse)
Status
Functioni
Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
GO - Molecular functioni
- bHLH transcription factor binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- double-stranded DNA binding Source: MGI
- enzyme binding Source: MGI
- histone deacetylase binding Source: MGI
- identical protein binding Source: MGI
- protein-containing complex binding Source: MGI
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: MGI
- protein kinase binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- cellular response to insulin stimulus Source: MGI
- glucose metabolic process Source: MGI
- late viral transcription Source: MGI
- lipid homeostasis Source: BHF-UCL
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- response to hypoxia Source: MGI
- response to UV Source: BHF-UCL
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-9018519, Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: Upstream stimulatory factor 1Alternative name(s): Major late transcription factor 1 |
Gene namesi | Name:Usf1 Synonyms:Usf |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99542, Usf1 |
VEuPathDBi | HostDB:ENSMUSG00000026641 |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- chromatin Source: MGI
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127497 | 1 – 310 | Upstream stimulatory factor 1Add BLAST | 310 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 306 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
EPDi | Q61069 |
jPOSTi | Q61069 |
MaxQBi | Q61069 |
PaxDbi | Q61069 |
PRIDEi | Q61069 |
ProteomicsDBi | 297901 |
PTM databases
iPTMneti | Q61069 |
PhosphoSitePlusi | Q61069 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026641, Expressed in ganglionic eminence and 142 other tissues |
Genevisiblei | Q61069, MM |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2).
Binary interactionsi
Q61069
With | #Exp. | IntAct |
---|---|---|
Stat1 [P42225] | 2 | EBI-2325635,EBI-647118 |
GO - Molecular functioni
- bHLH transcription factor binding Source: MGI
- enzyme binding Source: MGI
- histone deacetylase binding Source: MGI
- identical protein binding Source: MGI
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: MGI
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204464, 5 interactors |
ComplexPortali | CPX-3085, USF1 upstream stimulatory factor complex CPX-3086, USF1-USF2 upstream stimulatory factor complex |
IntActi | Q61069, 1 interactor |
STRINGi | 10090.ENSMUSP00000125729 |
Miscellaneous databases
RNActi | Q61069, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 199 – 254 | bHLHPROSITE-ProRule annotationAdd BLAST | 56 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 26 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 171 – 209 | DisorderedSequence analysisAdd BLAST | 39 | |
Regioni | 271 – 292 | Leucine-zipperAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 190 – 209 | Basic and acidic residuesSequence analysisAdd BLAST | 20 |
Phylogenomic databases
eggNOGi | KOG1318, Eukaryota |
GeneTreei | ENSGT00940000157083 |
HOGENOMi | CLU_070485_0_0_1 |
InParanoidi | Q61069 |
OMAi | SKTIPEC |
OrthoDBi | 1345445at2759 |
PhylomeDBi | Q61069 |
TreeFami | TF323338 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q61069-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKGQQKTAET EEGTVQIQEG AVATGEDPTS VAIASIQSAA TFPDPNVKYV
60 70 80 90 100
FRTENGGQVM YRVIQVSEGQ LDGQTEGSGA ISGYPATQSM TQAVIQGAFT
110 120 130 140 150
SDDAVDTEGA AAETHYTYFP STAVGDGSGG TTSGSTTAVV TTQGSEALLG
160 170 180 190 200
QATPPSTGQF FVMMSPQEVL QGGSQRSIAP RTHPYSPKSE APRTTRDEKR
210 220 230 240 250
RAQHNEVERR RRDKINNWIV QLSKIIPDCS MESTKSGQSK GGILSKACDY
260 270 280 290 300
IQELRQSNHR LSEELQGLDQ LQLDNDVLRQ QVEDLKNKNL LLRAQLRHHG
310
LEVVIKNDSN
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q722 | E9Q722_MOUSE | Upstream stimulatory factor 1 | Usf1 | 296 | Annotation score: | ||
E0CYP7 | E0CYP7_MOUSE | Upstream stimulatory factor 1 | Usf1 | 148 | Annotation score: | ||
F8WGH6 | F8WGH6_MOUSE | Upstream stimulatory factor 1 | Usf1 | 48 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U41741 Genomic DNA Translation: AAC52921.1 |
CCDSi | CCDS15495.1 |
RefSeqi | NP_001292605.1, NM_001305676.1 NP_001292606.1, NM_001305677.1 NP_033506.1, NM_009480.3 |
Genome annotation databases
Ensembli | ENSMUST00000161241; ENSMUSP00000125729; ENSMUSG00000026641 ENSMUST00000167546; ENSMUSP00000128913; ENSMUSG00000026641 |
GeneIDi | 22278 |
KEGGi | mmu:22278 |
UCSCi | uc007doj.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U41741 Genomic DNA Translation: AAC52921.1 |
CCDSi | CCDS15495.1 |
RefSeqi | NP_001292605.1, NM_001305676.1 NP_001292606.1, NM_001305677.1 NP_033506.1, NM_009480.3 |
3D structure databases
SMRi | Q61069 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204464, 5 interactors |
ComplexPortali | CPX-3085, USF1 upstream stimulatory factor complex CPX-3086, USF1-USF2 upstream stimulatory factor complex |
IntActi | Q61069, 1 interactor |
STRINGi | 10090.ENSMUSP00000125729 |
PTM databases
iPTMneti | Q61069 |
PhosphoSitePlusi | Q61069 |
Proteomic databases
EPDi | Q61069 |
jPOSTi | Q61069 |
MaxQBi | Q61069 |
PaxDbi | Q61069 |
PRIDEi | Q61069 |
ProteomicsDBi | 297901 |
Protocols and materials databases
Antibodypediai | 3773, 373 antibodies from 36 providers |
DNASUi | 22278 |
Genome annotation databases
Ensembli | ENSMUST00000161241; ENSMUSP00000125729; ENSMUSG00000026641 ENSMUST00000167546; ENSMUSP00000128913; ENSMUSG00000026641 |
GeneIDi | 22278 |
KEGGi | mmu:22278 |
UCSCi | uc007doj.2, mouse |
Organism-specific databases
CTDi | 7391 |
MGIi | MGI:99542, Usf1 |
VEuPathDBi | HostDB:ENSMUSG00000026641 |
Phylogenomic databases
eggNOGi | KOG1318, Eukaryota |
GeneTreei | ENSGT00940000157083 |
HOGENOMi | CLU_070485_0_0_1 |
InParanoidi | Q61069 |
OMAi | SKTIPEC |
OrthoDBi | 1345445at2759 |
PhylomeDBi | Q61069 |
TreeFami | TF323338 |
Enzyme and pathway databases
Reactomei | R-MMU-9018519, Estrogen-dependent gene expression |
Miscellaneous databases
BioGRID-ORCSi | 22278, 0 hits in 51 CRISPR screens |
ChiTaRSi | Usf1, mouse |
PROi | PR:Q61069 |
RNActi | Q61069, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026641, Expressed in ganglionic eminence and 142 other tissues |
Genevisiblei | Q61069, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | USF1_MOUSE | |
Accessioni | Q61069Primary (citable) accession number: Q61069 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot