UniProtKB - Q61048 (WBP4_MOUSE)
WW domain-binding protein 4
Wbp4
Functioni
Involved in pre-mRNA splicing as a component of the spliceosome. May play a role in cross-intron bridging of U1 and U2 snRNPs in the mammalian A complex.
1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 11 – 42 | Matrin-typePROSITE-ProRule annotationAdd BLAST | 32 |
GO - Molecular functioni
- proline-rich region binding Source: MGI
- RNA binding Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- mRNA cis splicing, via spliceosome Source: UniProtKB
- mRNA splicing, via spliceosome Source: UniProtKB
- RNA splicing Source: GO_Central
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: WW domain-binding protein 4Short name: WBP-4 Alternative name(s): Formin-binding protein 21 WW domain-containing-binding protein 4 |
Gene namesi | Name:Wbp4 Synonyms:Fbp21, Fnbp21 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109568, Wbp4 |
VEuPathDBi | HostDB:ENSMUSG00000022023 |
Subcellular locationi
Nucleus
- Nucleus By similarity
- Nucleus speckle PROSITE-ProRule annotation1 Publication
Nucleus
- nuclear speck Source: UniProtKB
- nucleus Source: MGI
- precatalytic spliceosome Source: GO_Central
- spliceosomal complex Source: MGI
- U2-type precatalytic spliceosome Source: UniProtKB
Keywords - Cellular componenti
Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076066 | 1 – 376 | WW domain-binding protein 4Add BLAST | 376 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 228 | PhosphoserineBy similarity | 1 | |
Modified residuei | 230 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q61048 |
MaxQBi | Q61048 |
PaxDbi | Q61048 |
PRIDEi | Q61048 |
ProteomicsDBi | 297634 |
PTM databases
iPTMneti | Q61048 |
PhosphoSitePlusi | Q61048 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000022023, Expressed in pancreas and 313 other tissues |
ExpressionAtlasi | Q61048, baseline and differential |
Genevisiblei | Q61048, MM |
Interactioni
Subunit structurei
Component of the spliceosome B complex (PubMed:9724750). Associated with U2 snRNPs. Binds splicing factors SNRPB, SNRPC and SF1 (PubMed:9724750).
Interacts via the WW domains with the Pro-rich domains of KHDRBS1/SAM68 (PubMed:10748127).
Interacts via the WW domains with the Pro-rich domains of WBP11 (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- proline-rich region binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204548, 4 interactors |
ELMi | Q61048 |
IntActi | Q61048, 1 interactor |
MINTi | Q61048 |
STRINGi | 10090.ENSMUSP00000022601 |
Miscellaneous databases
RNActi | Q61048, protein |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 123 – 156 | WW 1PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 164 – 197 | WW 2PROSITE-ProRule annotationAdd BLAST | 34 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 94 – 127 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 192 – 324 | DisorderedSequence analysisAdd BLAST | 133 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 94 – 110 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 213 – 268 | Basic and acidic residuesSequence analysisAdd BLAST | 56 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 11 – 42 | Matrin-typePROSITE-ProRule annotationAdd BLAST | 32 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0150, Eukaryota |
GeneTreei | ENSGT00390000013956 |
HOGENOMi | CLU_050927_1_0_1 |
InParanoidi | Q61048 |
OMAi | ANPYGVW |
OrthoDBi | 1585242at2759 |
PhylomeDBi | Q61048 |
TreeFami | TF316671 |
Family and domain databases
CDDi | cd00201, WW, 2 hits |
InterProi | View protein in InterPro IPR000690, Matrin/U1-C_Znf_C2H2 IPR003604, Matrin/U1-like-C_Znf_C2H2 IPR013085, U1-CZ_Znf_C2H2 IPR040023, WBP4 IPR001202, WW_dom IPR036020, WW_dom_sf IPR036236, Znf_C2H2_sf |
PANTHERi | PTHR13173, PTHR13173, 1 hit |
Pfami | View protein in Pfam PF00397, WW, 2 hits PF06220, zf-U1, 1 hit |
SMARTi | View protein in SMART SM00456, WW, 2 hits SM00451, ZnF_U1, 1 hit |
SUPFAMi | SSF51045, SSF51045, 2 hits SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS01159, WW_DOMAIN_1, 1 hit PS50020, WW_DOMAIN_2, 2 hits PS50171, ZF_MATRIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MADYWKSQPK KFCDYCKCWI ADNRPSVEFH ERGKNHKENV ARRISEIKQK
60 70 80 90 100
SLDKAKEEEK ASKEFAAMEA AALKAYQEDL KRLGLPLPSD ISEPTVSPVI
110 120 130 140 150
STVQPTPTSN QQKEKKKKKK KKEASKGGWV EGVTADGHCY YYDLITGASQ
160 170 180 190 200
WEKPEGFQGN LKKTAAKAVW VEGLSEDGYT YYYNTETGES KWEKPEDFIP
210 220 230 240 250
HGGDVLSSKD SGKLPDTLED AKSSDSHSDS EGEQKKAGEA STETKKLIIK
260 270 280 290 300
FKEKNKSTEK RIGPEIQKEK STPKQNPSNT NEEKPKTLKK STNPYGEWQE
310 320 330 340 350
IKQEAESQEE VDLELPSTEG ECLSTSEAGV GEIKVVFKEK TVSSLGVAAD
360 370
GVAPVFKKRR LENGKSRNLR QRGDDE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A2I3BQA9 | A0A2I3BQA9_MOUSE | WW domain-binding protein 4 | Wbp4 | 315 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 80 – 83 | LKRL → SEKA in AAC34810 (PubMed:9724750).Curated | 4 | |
Sequence conflicti | 155 | E → K in AAH34851 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF071184 mRNA Translation: AAC34810.1 AK019160 mRNA Translation: BAB31575.3 AK135215 mRNA Translation: BAE22462.1 AK160559 mRNA Translation: BAE35873.1 AK162009 mRNA Translation: BAE36679.1 CH466535 Genomic DNA Translation: EDL35764.1 BC034851 mRNA Translation: AAH34851.1 |
CCDSi | CCDS27300.1 |
PIRi | S64714 |
RefSeqi | NP_061235.2, NM_018765.3 |
Genome annotation databases
Ensembli | ENSMUST00000022601; ENSMUSP00000022601; ENSMUSG00000022023 |
GeneIDi | 22380 |
KEGGi | mmu:22380 |
UCSCi | uc007uta.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF071184 mRNA Translation: AAC34810.1 AK019160 mRNA Translation: BAB31575.3 AK135215 mRNA Translation: BAE22462.1 AK160559 mRNA Translation: BAE35873.1 AK162009 mRNA Translation: BAE36679.1 CH466535 Genomic DNA Translation: EDL35764.1 BC034851 mRNA Translation: AAH34851.1 |
CCDSi | CCDS27300.1 |
PIRi | S64714 |
RefSeqi | NP_061235.2, NM_018765.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 204548, 4 interactors |
ELMi | Q61048 |
IntActi | Q61048, 1 interactor |
MINTi | Q61048 |
STRINGi | 10090.ENSMUSP00000022601 |
PTM databases
iPTMneti | Q61048 |
PhosphoSitePlusi | Q61048 |
Proteomic databases
EPDi | Q61048 |
MaxQBi | Q61048 |
PaxDbi | Q61048 |
PRIDEi | Q61048 |
ProteomicsDBi | 297634 |
Protocols and materials databases
Antibodypediai | 42181, 51 antibodies from 12 providers |
DNASUi | 22380 |
Genome annotation databases
Ensembli | ENSMUST00000022601; ENSMUSP00000022601; ENSMUSG00000022023 |
GeneIDi | 22380 |
KEGGi | mmu:22380 |
UCSCi | uc007uta.1, mouse |
Organism-specific databases
CTDi | 11193 |
MGIi | MGI:109568, Wbp4 |
VEuPathDBi | HostDB:ENSMUSG00000022023 |
Phylogenomic databases
eggNOGi | KOG0150, Eukaryota |
GeneTreei | ENSGT00390000013956 |
HOGENOMi | CLU_050927_1_0_1 |
InParanoidi | Q61048 |
OMAi | ANPYGVW |
OrthoDBi | 1585242at2759 |
PhylomeDBi | Q61048 |
TreeFami | TF316671 |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway |
Miscellaneous databases
BioGRID-ORCSi | 22380, 10 hits in 68 CRISPR screens |
ChiTaRSi | Wbp4, mouse |
PROi | PR:Q61048 |
RNActi | Q61048, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022023, Expressed in pancreas and 313 other tissues |
ExpressionAtlasi | Q61048, baseline and differential |
Genevisiblei | Q61048, MM |
Family and domain databases
CDDi | cd00201, WW, 2 hits |
InterProi | View protein in InterPro IPR000690, Matrin/U1-C_Znf_C2H2 IPR003604, Matrin/U1-like-C_Znf_C2H2 IPR013085, U1-CZ_Znf_C2H2 IPR040023, WBP4 IPR001202, WW_dom IPR036020, WW_dom_sf IPR036236, Znf_C2H2_sf |
PANTHERi | PTHR13173, PTHR13173, 1 hit |
Pfami | View protein in Pfam PF00397, WW, 2 hits PF06220, zf-U1, 1 hit |
SMARTi | View protein in SMART SM00456, WW, 2 hits SM00451, ZnF_U1, 1 hit |
SUPFAMi | SSF51045, SSF51045, 2 hits SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS01159, WW_DOMAIN_1, 1 hit PS50020, WW_DOMAIN_2, 2 hits PS50171, ZF_MATRIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | WBP4_MOUSE | |
Accessioni | Q61048Primary (citable) accession number: Q61048 Secondary accession number(s): Q3TUU8, Q8K1Z9, Q9CS45 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 146 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot