UniProtKB - Q61039 (HAND2_MOUSE)
Heart- and neural crest derivatives-expressed protein 2
Hand2
Functioni
Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3'.
4 PublicationsGO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: BHF-UCL
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: BHF-UCL
- DNA-binding transcription factor binding Source: MGI
- E-box binding Source: MGI
- minor groove of adenine-thymine-rich DNA binding Source: MGI
- protein homodimerization activity Source: BHF-UCL
- RNA polymerase II-specific DNA-binding transcription factor binding Source: BHF-UCL
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription coactivator binding Source: BHF-UCL
GO - Biological processi
- adult heart development Source: MGI
- angiogenesis Source: UniProtKB-KW
- apoptotic process involved in heart morphogenesis Source: MGI
- cardiac muscle cell differentiation Source: MGI
- cardiac neural crest cell development involved in outflow tract morphogenesis Source: BHF-UCL
- cardiac neural crest cell migration involved in outflow tract morphogenesis Source: MGI
- cardiac right ventricle formation Source: BHF-UCL
- cardiac right ventricle morphogenesis Source: MGI
- cartilage morphogenesis Source: MGI
- cell proliferation involved in outflow tract morphogenesis Source: MGI
- cellular response to organic cyclic compound Source: Ensembl
- cellular response to retinoic acid Source: Ensembl
- coronary artery morphogenesis Source: MGI
- determination of heart left/right asymmetry Source: MGI
- developmental process Source: GO_Central
- embryonic digit morphogenesis Source: MGI
- embryonic skeletal system development Source: MGI
- heart development Source: MGI
- heart looping Source: MGI
- heart morphogenesis Source: MGI
- in utero embryonic development Source: MGI
- mesenchymal cell proliferation Source: MGI
- mesenchyme development Source: MGI
- negative regulation of apoptotic process Source: MGI
- negative regulation of cardiac muscle cell apoptotic process Source: BHF-UCL
- negative regulation of DNA binding Source: MGI
- negative regulation of DNA-binding transcription factor activity Source: MGI
- negative regulation of gene expression Source: MGI
- negative regulation of osteoblast differentiation Source: MGI
- neural crest cell development Source: MGI
- odontogenesis of dentin-containing tooth Source: MGI
- peripheral nervous system neuron development Source: MGI
- positive regulation of cardiac muscle hypertrophy Source: MGI
- positive regulation of ERK1 and ERK2 cascade Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of p38MAPK cascade Source: MGI
- positive regulation of semaphorin-plexin signaling pathway involved in outflow tract morphogenesis Source: BHF-UCL
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- positive regulation of transcription from RNA polymerase II promoter involved in norepinephrine biosynthetic process Source: BHF-UCL
- positive regulation of transcription regulatory region DNA binding Source: MGI
- primary palate development Source: MGI
- regulation of secondary heart field cardioblast proliferation Source: BHF-UCL
- regulation of tissue remodeling Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- roof of mouth development Source: MGI
- suckling behavior Source: MGI
- sympathetic nervous system development Source: MGI
- thymus development Source: BHF-UCL
- tongue development Source: MGI
- visceral serous pericardium development Source: MGI
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Biological process | Angiogenesis, Differentiation, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Heart- and neural crest derivatives-expressed protein 2Alternative name(s): Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2 Short name: dHAND Helix-loop-helix transcription factor expressed in embryo and deciduum Thing-2 |
Gene namesi | Name:Hand2 Synonyms:Dhand, Hed, Thing2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:103580, Hand2 |
VEuPathDBi | HostDB:ENSMUSG00000038193 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: MGI
Other locations
- chromatin Source: MGI
- protein-containing complex Source: MGI
- transcription regulator complex Source: BHF-UCL
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127192 | 1 – 217 | Heart- and neural crest derivatives-expressed protein 2Add BLAST | 217 |
Proteomic databases
jPOSTi | Q61039 |
MaxQBi | Q61039 |
PaxDbi | Q61039 |
PRIDEi | Q61039 |
ProteomicsDBi | 271390 |
PTM databases
iPTMneti | Q61039 |
PhosphoSitePlusi | Q61039 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000038193, Expressed in decidua and 188 other tissues |
Genevisiblei | Q61039, MM |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Forms homodimers and heterodimers with TCF3 gene products E12 and E47, HAND1 and HEY1, HEY2 and HEYL (hairy-related transcription factors).
1 PublicationBinary interactionsi
Q61039
With | #Exp. | IntAct |
---|---|---|
Hand1 [Q64279] | 3 | EBI-81388,EBI-81361 |
Mcf2 [Q99N72] | 3 | EBI-81388,EBI-641874 |
Tcf3 [P15806] | 2 | EBI-81388,EBI-81370 |
GO - Molecular functioni
- DNA-binding transcription factor binding Source: MGI
- protein homodimerization activity Source: BHF-UCL
- RNA polymerase II-specific DNA-binding transcription factor binding Source: BHF-UCL
- transcription coactivator binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 200205, 7 interactors |
IntActi | Q61039, 8 interactors |
STRINGi | 10090.ENSMUSP00000044983 |
Miscellaneous databases
RNActi | Q61039, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 99 – 151 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 76 – 116 | DisorderedSequence analysisAdd BLAST | 41 |
Phylogenomic databases
eggNOGi | KOG4029, Eukaryota |
GeneTreei | ENSGT00940000160772 |
HOGENOMi | CLU_119591_0_0_1 |
InParanoidi | Q61039 |
OMAi | CHEDSGA |
OrthoDBi | 1449816at2759 |
PhylomeDBi | Q61039 |
TreeFami | TF315153 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSLVGGFPHH PVVHHEGYPF AAAAAAAAAA AASRCSHEEN PYFHGWLIGH
60 70 80 90 100
PEMSPPDYSM ALSYSPEYAS GAAGLDHSHY GGVPPGAGPP GLGGPRPVKR
110 120 130 140 150
RGTANRKERR RTQSINSAFA ELRECIPNVP ADTKLSKIKT LRLATSYIAY
160 170 180 190 200
LMDLLAKDDQ NGEAEAFKAE IKKTDVKEEK RKKELNEILK STVSSNDKKT
210
KGRTGWPQHV WALELKQ
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 92 – 93 | LG → TA in AAA86274 (PubMed:7671815).Curated | 2 | |
Sequence conflicti | 156 | A → P in AAC52338 (PubMed:8533092).Curated | 1 | |
Sequence conflicti | 166 | A → G in AAA86274 (PubMed:7671815).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U40039 Genomic DNA Translation: AAC52338.1 Frameshift. AK035160 mRNA Translation: BAC28965.1 U43715 mRNA Translation: AAA86274.1 Frameshift. |
CCDSi | CCDS22315.1 |
RefSeqi | NP_034532.3, NM_010402.4 |
Genome annotation databases
Ensembli | ENSMUST00000040104; ENSMUSP00000044983; ENSMUSG00000038193 |
GeneIDi | 15111 |
KEGGi | mmu:15111 |
UCSCi | uc009lss.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U40039 Genomic DNA Translation: AAC52338.1 Frameshift. AK035160 mRNA Translation: BAC28965.1 U43715 mRNA Translation: AAA86274.1 Frameshift. |
CCDSi | CCDS22315.1 |
RefSeqi | NP_034532.3, NM_010402.4 |
3D structure databases
AlphaFoldDBi | Q61039 |
SMRi | Q61039 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200205, 7 interactors |
IntActi | Q61039, 8 interactors |
STRINGi | 10090.ENSMUSP00000044983 |
PTM databases
iPTMneti | Q61039 |
PhosphoSitePlusi | Q61039 |
Proteomic databases
jPOSTi | Q61039 |
MaxQBi | Q61039 |
PaxDbi | Q61039 |
PRIDEi | Q61039 |
ProteomicsDBi | 271390 |
Protocols and materials databases
Antibodypediai | 28534, 292 antibodies from 30 providers |
DNASUi | 15111 |
Genome annotation databases
Ensembli | ENSMUST00000040104; ENSMUSP00000044983; ENSMUSG00000038193 |
GeneIDi | 15111 |
KEGGi | mmu:15111 |
UCSCi | uc009lss.2, mouse |
Organism-specific databases
CTDi | 9464 |
MGIi | MGI:103580, Hand2 |
VEuPathDBi | HostDB:ENSMUSG00000038193 |
Phylogenomic databases
eggNOGi | KOG4029, Eukaryota |
GeneTreei | ENSGT00940000160772 |
HOGENOMi | CLU_119591_0_0_1 |
InParanoidi | Q61039 |
OMAi | CHEDSGA |
OrthoDBi | 1449816at2759 |
PhylomeDBi | Q61039 |
TreeFami | TF315153 |
Miscellaneous databases
BioGRID-ORCSi | 15111, 2 hits in 73 CRISPR screens |
ChiTaRSi | Hand2, mouse |
PROi | PR:Q61039 |
RNActi | Q61039, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038193, Expressed in decidua and 188 other tissues |
Genevisiblei | Q61039, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HAND2_MOUSE | |
Accessioni | Q61039Primary (citable) accession number: Q61039 Secondary accession number(s): Q61100 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | May 10, 2004 | |
Last modified: | May 25, 2022 | |
This is version 191 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot