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Entry version 202 (25 May 2022)
Sequence version 2 (10 May 2004)
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Protein

Serine/threonine-protein kinase PAK 3

Gene

Pak3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity (PubMed:25851601).

Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses; this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain (MLC) (PubMed:15800193).

6 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-436 and allows the kinase domain to adopt an active structure (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei312ATPCurated1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei402Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi289 – 297ATPPROSITE-ProRule annotation9

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionAllosteric enzyme, Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
2.7.11.1, 3474

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-MMU-202433, Generation of second messenger molecules
R-MMU-389359, CD28 dependent Vav1 pathway
R-MMU-3928664, Ephrin signaling
R-MMU-399954, Sema3A PAK dependent Axon repulsion
R-MMU-5218920, VEGFR2 mediated vascular permeability
R-MMU-5621575, CD209 (DC-SIGN) signaling
R-MMU-5627123, RHO GTPases activate PAKs
R-MMU-5687128, MAPK6/MAPK4 signaling
R-MMU-9013148, CDC42 GTPase cycle
R-MMU-9013149, RAC1 GTPase cycle
R-MMU-9013409, RHOJ GTPase cycle
R-MMU-9013420, RHOU GTPase cycle

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Serine/threonine-protein kinase PAK 3 (EC:2.7.11.1)
Alternative name(s):
Beta-PAK
CDC42/RAC effector kinase PAK-B
p21-activated kinase 3
Short name:
PAK-3
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Pak3
Synonyms:Pak-3, Pakb, Stk4
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome X

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:1339656, Pak3

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
HostDB:ENSMUSG00000031284

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Mice show significant abnormalities in synaptic plasticity as well as deficiencies in learning and memory. Pak1 and Pak3 double knockout mice display reduced brains characterized by simplified neuronal dendrites/axons and reduced synapse density.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi67R → C: Decreases interaction of PAK3 with CDC42 but increases interaction with RAC1. 1 Publication1
Mutagenesisi78H → L: When expressed in hippocampal neurons, strongly decreases the number of dendritic spines; when associated with L-81 and R-312. 1 Publication1
Mutagenesisi81H → L: When expressed in hippocampal neurons, strongly decreases the number of dendritic spines; when associated with L-78 and R-312. 1 Publication1
Mutagenesisi312K → A: Loss of kinase activity. 1 Publication1
Mutagenesisi312K → R: When expressed in hippocampal neurons, decreases the number of dendritic spines. Strong decrease in the number of dendritic spines; when associated with L-78 and L-81. 1 Publication1
Mutagenesisi436T → E: When expressed in hippocampal neurons, increases the density of both spines and dendritic protrusions. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000864701 – 559Serine/threonine-protein kinase PAK 3Add BLAST559

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei2PhosphoserineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei50Phosphoserine; by autocatalysisBy similarity1
Modified residuei154Phosphoserine; by autocatalysisBy similarity1
Modified residuei186PhosphoserineCombined sources1
Modified residuei436Phosphothreonine; by autocatalysisBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Autophosphorylated when activated by CDC42/p21.
Neddylated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
Q61036

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
Q61036

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q61036

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q61036

PRoteomics IDEntifications database

More...
PRIDEi
Q61036

ProteomicsDB: a multi-organism proteome resource

More...
ProteomicsDBi
287772 [Q61036-1]
287773 [Q61036-2]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q61036

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q61036

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSMUSG00000031284, Expressed in midbrain and 264 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
Q61036, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
Q61036, MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Shows highly specific binding to the SH3 domains of phospholipase C-gamma and of adapter protein NCK.

Interacts with the C-terminal of APP.

Interacts with ARHGEF6 and ARHGEF7 (By similarity).

Interacts with GIT1 and GIT2 (By similarity).

By similarity

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
202021, 16 interactors

Database of interacting proteins

More...
DIPi
DIP-447N

Protein interaction database and analysis system

More...
IntActi
Q61036, 6 interactors

Molecular INTeraction database

More...
MINTi
Q61036

STRING: functional protein association networks

More...
STRINGi
10090.ENSMUSP00000108485

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
Q61036, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1559
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

AlphaFold Protein Structure Database

More...
AlphaFoldDBi
Q61036

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q61036

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
Q61036

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini70 – 83CRIBPROSITE-ProRule annotationAdd BLAST14
Domaini283 – 534Protein kinasePROSITE-ProRule annotationAdd BLAST252

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 70DisorderedSequence analysisAdd BLAST70
Regioni65 – 150Autoregulatory regionBy similarityAdd BLAST86
Regioni65 – 123GTPase-bindingBy similarityAdd BLAST59
Regioni84 – 282LinkerAdd BLAST199
Regioni171 – 215DisorderedSequence analysisAdd BLAST45

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi18 – 32Polar residuesSequence analysisAdd BLAST15
Compositional biasi56 – 70Basic and acidic residuesSequence analysisAdd BLAST15
Compositional biasi184 – 201Acidic residuesSequence analysisAdd BLAST18

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

SH3-binding

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG0578, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00950000182988

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_000288_26_6_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q61036

Identification of Orthologs from Complete Genome Data

More...
OMAi
WYDASAT

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q61036

TreeFam database of animal gene trees

More...
TreeFami
TF105351

Family and domain databases

Conserved Domains Database

More...
CDDi
cd01093, CRIB_PAK_like, 1 hit
cd06656, STKc_PAK3, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.90.810.10, 1 hit

Intrinsically Disordered proteins with Extensive Annotations and Literature

More...
IDEALi
IID50053

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR000095, CRIB_dom
IPR036936, CRIB_dom_sf
IPR011009, Kinase-like_dom_sf
IPR033923, PAK_BD
IPR000719, Prot_kinase_dom
IPR017441, Protein_kinase_ATP_BS
IPR008271, Ser/Thr_kinase_AS
IPR035063, STK_PAK3

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00786, PBD, 1 hit
PF00069, Pkinase, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00285, PBD, 1 hit
SM00220, S_TKc, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF56112, SSF56112, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50108, CRIB, 1 hit
PS00107, PROTEIN_KINASE_ATP, 1 hit
PS50011, PROTEIN_KINASE_DOM, 1 hit
PS00108, PROTEIN_KINASE_ST, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q61036-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSDSLDNEEK PPAPPLRMNS NNRDSSALNH SSKPLPMAPE EKNKKARLRS
60 70 80 90 100
IFPGGGDKTN KKKEKERPEI SLPSDFEHTI HVGFDAVTGE FTPDLYGSQM
110 120 130 140 150
CPGKLPEGIP EQWARLLQTS NITKLEQKKN PQAVLDVLKF YDSKETVNNQ
160 170 180 190 200
KYMSFTSGDK SAHGYIAAHQ SNTKTASEPP LAPPVSEEED EEEEEEEDDN
210 220 230 240 250
EPPPVIAPRP EHTKSIYTRS VVESIASPAA PNKEDIPPSA ENANSTTLYR
260 270 280 290 300
NTDRQRKKSK MTDEEILEKL RSIVSVGDPK KKYTRFEKIG QGASGTVYTA
310 320 330 340 350
LDIATGQEVA IKQMNLQQQP KKELIINEIL VMRENKNPNI VNYLDSYLVG
360 370 380 390 400
DELWVVMEYL AGGSLTDVVT ETCMDEGQIA AVCRECLQAL DFLHSNQVIH
410 420 430 440 450
RDIKSDNILL GMDGSVKLTD FGFCAQITPE QSKRSTMVGT PYWMAPEVVT
460 470 480 490 500
RKAYGPKVDI WSLGIMAIEM VEGEPPYLNE NPLRALYLIA TNGTPELQNP
510 520 530 540 550
ERLSAVFRDF LNRCLEMDVD RRGSAKELLQ HPFLKLAKPL SSLTPLIIAA

KEAIKNSSR
Length:559
Mass (Da):62,398
Last modified:May 10, 2004 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i9AD07B0328F0B08C
GO
Isoform 2 (identifier: Q61036-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     93-107: Missing.

Show »
Length:544
Mass (Da):60,781
Checksum:i72E323575DC18E0F
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A3KGC5A3KGC5_MOUSE
Non-specific serine/threonine prote...
Pak3
366Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A3KGC3A3KGC3_MOUSE
Non-specific serine/threonine prote...
Pak3
155Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A3KGC4A3KGC4_MOUSE
Non-specific serine/threonine prote...
Pak3
195Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A3KGC2A3KGC2_MOUSE
Serine/threonine-protein kinase PAK...
Pak3
23Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti176A → G in AAC52354 (PubMed:7559398).Curated1
Sequence conflicti286F → L (PubMed:7559398).Curated1
Sequence conflicti286F → L (PubMed:10352232).Curated1
Sequence conflicti376E → V in AAC52354 (PubMed:7559398).Curated1
Sequence conflicti508R → H in AAC52354 (PubMed:7559398).Curated1
Sequence conflicti540L → M in AAC31969 (PubMed:10352232).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_01024393 – 107Missing in isoform 2. 4 PublicationsAdd BLAST15

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U39738 mRNA Translation: AAC52354.1
AF082297 mRNA Translation: AAC31969.1
AJ496262 mRNA Translation: CAD42790.1
AJ496263 mRNA Translation: CAD42791.1
AK031853 mRNA Translation: BAC27580.1
BC053403 mRNA Translation: AAH53403.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS30454.1 [Q61036-2]
CCDS57779.1 [Q61036-1]

Protein sequence database of the Protein Information Resource

More...
PIRi
I49376

NCBI Reference Sequences

More...
RefSeqi
NP_001181977.1, NM_001195048.1 [Q61036-1]
NP_001181978.1, NM_001195049.1 [Q61036-2]
NP_032804.2, NM_008778.3 [Q61036-2]
XP_006528815.1, XM_006528752.3 [Q61036-1]
XP_006528816.1, XM_006528753.3 [Q61036-2]
XP_006528817.1, XM_006528754.3 [Q61036-2]
XP_011246092.1, XM_011247790.2 [Q61036-2]
XP_017173912.1, XM_017318423.1

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSMUST00000033640; ENSMUSP00000033640; ENSMUSG00000031284 [Q61036-1]
ENSMUST00000112863; ENSMUSP00000108484; ENSMUSG00000031284 [Q61036-1]
ENSMUST00000112864; ENSMUSP00000108485; ENSMUSG00000031284 [Q61036-2]
ENSMUST00000112865; ENSMUSP00000108486; ENSMUSG00000031284 [Q61036-2]
ENSMUST00000112868; ENSMUSP00000108489; ENSMUSG00000031284 [Q61036-2]
ENSMUST00000172330; ENSMUSP00000126562; ENSMUSG00000031284 [Q61036-2]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
18481

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mmu:18481

UCSC genome browser

More...
UCSCi
uc009umg.2, mouse [Q61036-1]
uc009umh.2, mouse [Q61036-2]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39738 mRNA Translation: AAC52354.1
AF082297 mRNA Translation: AAC31969.1
AJ496262 mRNA Translation: CAD42790.1
AJ496263 mRNA Translation: CAD42791.1
AK031853 mRNA Translation: BAC27580.1
BC053403 mRNA Translation: AAH53403.1
CCDSiCCDS30454.1 [Q61036-2]
CCDS57779.1 [Q61036-1]
PIRiI49376
RefSeqiNP_001181977.1, NM_001195048.1 [Q61036-1]
NP_001181978.1, NM_001195049.1 [Q61036-2]
NP_032804.2, NM_008778.3 [Q61036-2]
XP_006528815.1, XM_006528752.3 [Q61036-1]
XP_006528816.1, XM_006528753.3 [Q61036-2]
XP_006528817.1, XM_006528754.3 [Q61036-2]
XP_011246092.1, XM_011247790.2 [Q61036-2]
XP_017173912.1, XM_017318423.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EESNMR-B63-123[»]
AlphaFoldDBiQ61036
SMRiQ61036
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi202021, 16 interactors
DIPiDIP-447N
IntActiQ61036, 6 interactors
MINTiQ61036
STRINGi10090.ENSMUSP00000108485

PTM databases

iPTMnetiQ61036
PhosphoSitePlusiQ61036

Proteomic databases

EPDiQ61036
jPOSTiQ61036
MaxQBiQ61036
PaxDbiQ61036
PRIDEiQ61036
ProteomicsDBi287772 [Q61036-1]
287773 [Q61036-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...
Antibodypediai
29512, 526 antibodies from 37 providers

The DNASU plasmid repository

More...
DNASUi
18481

Genome annotation databases

EnsembliENSMUST00000033640; ENSMUSP00000033640; ENSMUSG00000031284 [Q61036-1]
ENSMUST00000112863; ENSMUSP00000108484; ENSMUSG00000031284 [Q61036-1]
ENSMUST00000112864; ENSMUSP00000108485; ENSMUSG00000031284 [Q61036-2]
ENSMUST00000112865; ENSMUSP00000108486; ENSMUSG00000031284 [Q61036-2]
ENSMUST00000112868; ENSMUSP00000108489; ENSMUSG00000031284 [Q61036-2]
ENSMUST00000172330; ENSMUSP00000126562; ENSMUSG00000031284 [Q61036-2]
GeneIDi18481
KEGGimmu:18481
UCSCiuc009umg.2, mouse [Q61036-1]
uc009umh.2, mouse [Q61036-2]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
5063
MGIiMGI:1339656, Pak3
VEuPathDBiHostDB:ENSMUSG00000031284

Phylogenomic databases

eggNOGiKOG0578, Eukaryota
GeneTreeiENSGT00950000182988
HOGENOMiCLU_000288_26_6_1
InParanoidiQ61036
OMAiWYDASAT
PhylomeDBiQ61036
TreeFamiTF105351

Enzyme and pathway databases

BRENDAi2.7.11.1, 3474
ReactomeiR-MMU-202433, Generation of second messenger molecules
R-MMU-389359, CD28 dependent Vav1 pathway
R-MMU-3928664, Ephrin signaling
R-MMU-399954, Sema3A PAK dependent Axon repulsion
R-MMU-5218920, VEGFR2 mediated vascular permeability
R-MMU-5621575, CD209 (DC-SIGN) signaling
R-MMU-5627123, RHO GTPases activate PAKs
R-MMU-5687128, MAPK6/MAPK4 signaling
R-MMU-9013148, CDC42 GTPase cycle
R-MMU-9013149, RAC1 GTPase cycle
R-MMU-9013409, RHOJ GTPase cycle
R-MMU-9013420, RHOU GTPase cycle

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...
BioGRID-ORCSi
18481, 0 hits in 75 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
Pak3, mouse
EvolutionaryTraceiQ61036

Protein Ontology

More...
PROi
PR:Q61036
RNActiQ61036, protein

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSMUSG00000031284, Expressed in midbrain and 264 other tissues
ExpressionAtlasiQ61036, baseline and differential
GenevisibleiQ61036, MM

Family and domain databases

CDDicd01093, CRIB_PAK_like, 1 hit
cd06656, STKc_PAK3, 1 hit
Gene3Di3.90.810.10, 1 hit
IDEALiIID50053
InterProiView protein in InterPro
IPR000095, CRIB_dom
IPR036936, CRIB_dom_sf
IPR011009, Kinase-like_dom_sf
IPR033923, PAK_BD
IPR000719, Prot_kinase_dom
IPR017441, Protein_kinase_ATP_BS
IPR008271, Ser/Thr_kinase_AS
IPR035063, STK_PAK3
PfamiView protein in Pfam
PF00786, PBD, 1 hit
PF00069, Pkinase, 1 hit
SMARTiView protein in SMART
SM00285, PBD, 1 hit
SM00220, S_TKc, 1 hit
SUPFAMiSSF56112, SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50108, CRIB, 1 hit
PS00107, PROTEIN_KINASE_ATP, 1 hit
PS50011, PROTEIN_KINASE_DOM, 1 hit
PS00108, PROTEIN_KINASE_ST, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPAK3_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q61036
Secondary accession number(s): O88645, Q8K1R5, Q8K1R6
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 10, 2004
Last modified: May 25, 2022
This is version 202 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
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