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Protein

Asparagine synthetase [glutamine-hydrolyzing]

Gene

Asns

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Asparagine synthetase [glutamine-hydrolyzing] (Asns)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei97GlutamineBy similarity1
Binding sitei256ATP; via carbonyl oxygenBy similarity1
Binding sitei288ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei365Important for beta-aspartyl-AMP intermediate formationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi363 – 364ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Asparagine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-70614 Amino acid synthesis and interconversion (transamination)
UniPathwayi
UPA00134;UER00195

Protein family/group databases

MEROPSiC44.974

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:Asns
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1350929 Asns

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Mice carrying a gene trap insertion in the gene express 20% of the normal level of mRNA. The hypomorphic mutant displays a number of defects that mirror ASNSD syndrome, although the phenotype is milder. Mice have structural brain abnormalities, including reduced cortical thickness and enlarged ventricles. Mutant mice also show deficits in learning and memory. Mutant mice do not show abnormal motor activity or seizure activity.1 Publication

Chemistry databases

ChEMBLiCHEMBL3243906

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000569122 – 561Asparagine synthetase [glutamine-hydrolyzing]Add BLAST560

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei385N6-acetyllysineBy similarity1
Modified residuei545PhosphothreonineBy similarity1
Modified residuei557PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ61024
MaxQBiQ61024
PaxDbiQ61024
PeptideAtlasiQ61024
PRIDEiQ61024

PTM databases

iPTMnetiQ61024
PhosphoSitePlusiQ61024
SwissPalmiQ61024

Expressioni

Gene expression databases

BgeeiENSMUSG00000029752 Expressed in 303 organ(s), highest expression level in saliva-secreting gland
CleanExiMM_ASNS
ExpressionAtlasiQ61024 baseline and differential
GenevisibleiQ61024 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi205108, 2 interactors
IntActiQ61024, 3 interactors
MINTiQ61024
STRINGi10090.ENSMUSP00000031766

Structurei

3D structure databases

ProteinModelPortaliQ61024
SMRiQ61024
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 191Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST190
Domaini213 – 536Asparagine synthetaseAdd BLAST324

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 53Glutamine bindingBy similarity5
Regioni75 – 77Glutamine bindingBy similarity3

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG0571 Eukaryota
COG0367 LUCA
GeneTreeiENSGT00390000001994
HOGENOMiHOG000027493
HOVERGENiHBG003103
InParanoidiQ61024
KOiK01953
OMAiLFGYKYT
OrthoDBiEOG091G13BD
PhylomeDBiQ61024
TreeFamiTF300603

Family and domain databases

CDDicd01991 Asn_Synthase_B_C, 1 hit
cd00712 AsnB, 1 hit
Gene3Di3.40.50.620, 1 hit
3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR006426 Asn_synth_AEB
IPR001962 Asn_synthase
IPR033738 AsnB_N
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00733 Asn_synthase, 1 hit
PF13537 GATase_7, 1 hit
PIRSFiPIRSF001589 Asn_synthetase_glu-h, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01536 asn_synth_AEB, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q61024-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCGIWALFGS DDCLSVQCLS AMKIAHRGPD AFRFENVNGY TNCCFGFHRL
60 70 80 90 100
AVVDPLFGMQ PIRVRKYPYL WLCYNGEIYN HKALQQRFEF EYQTNVDGEI
110 120 130 140 150
ILHLYDKGGI EKTICMLDGV FAFILLDTAN KKVFLGRDTY GVRPLFKAMT
160 170 180 190 200
EDGFLAVCSE AKGLVSLKHS TTPFLKVEPF LPGHYEVLDL KPNGKVASVE
210 220 230 240 250
MVKYHHCTDE PLHAIYDSVE KLFPGFDLET VKNNLRILFD NAIKKRLMTD
260 270 280 290 300
RRIGCLLSGG LDSSLVAASL LKQLKEAQVQ YPLQTFAIGM EDSPDLLAAR
310 320 330 340 350
KVANYIGSEH HEVLFNSEEG IQALDEVIFS LETYDITTVR ASVGMYLISK
360 370 380 390 400
YIRKNTDSVV IFSGEGSDEL TQGYIYFHKA PSPEKAEEES ERLLKELYLF
410 420 430 440 450
DVLRADRTTA AHGLELRVPF LDHRFSSYYL SLPPDMRIPK NGIEKHLLRE
460 470 480 490 500
TFEDCNLLPK EILWRPKEAF SDGITSVKNS WFKILQDYVE HQVDDEMMSA
510 520 530 540 550
ASQKFPFNTP KTKEGYFYRQ IFERHYPGRA DWLTHYWMPK WINATDPSAR
560
TLTHYKSAAK A
Length:561
Mass (Da):64,283
Last modified:January 23, 2007 - v3
Checksum:i1981956B69E1F9F1
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YYR0D3YYR0_MOUSE
Asparagine synthetase [glutamine-hy...
Asns
158Annotation score:
D3Z028D3Z028_MOUSE
Asparagine synthetase [glutamine-hy...
Asns
127Annotation score:
D3Z0C2D3Z0C2_MOUSE
Asparagine synthetase [glutamine-hy...
Asns
223Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5W → G in BAC36254 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38940 mRNA Translation: AAA85125.1
AK076207 mRNA Translation: BAC36254.1
AK167524 mRNA Translation: BAE39594.1
BC005552 mRNA Translation: AAH05552.1
CCDSiCCDS19907.1
RefSeqiNP_036185.1, NM_012055.3
XP_006505155.1, XM_006505092.3
XP_006505156.1, XM_006505093.3
UniGeneiMm.2942

Genome annotation databases

EnsembliENSMUST00000031766; ENSMUSP00000031766; ENSMUSG00000029752
ENSMUST00000115542; ENSMUSP00000111204; ENSMUSG00000029752
GeneIDi27053
KEGGimmu:27053
UCSCiuc009axf.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38940 mRNA Translation: AAA85125.1
AK076207 mRNA Translation: BAC36254.1
AK167524 mRNA Translation: BAE39594.1
BC005552 mRNA Translation: AAH05552.1
CCDSiCCDS19907.1
RefSeqiNP_036185.1, NM_012055.3
XP_006505155.1, XM_006505092.3
XP_006505156.1, XM_006505093.3
UniGeneiMm.2942

3D structure databases

ProteinModelPortaliQ61024
SMRiQ61024
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205108, 2 interactors
IntActiQ61024, 3 interactors
MINTiQ61024
STRINGi10090.ENSMUSP00000031766

Chemistry databases

ChEMBLiCHEMBL3243906

Protein family/group databases

MEROPSiC44.974

PTM databases

iPTMnetiQ61024
PhosphoSitePlusiQ61024
SwissPalmiQ61024

Proteomic databases

EPDiQ61024
MaxQBiQ61024
PaxDbiQ61024
PeptideAtlasiQ61024
PRIDEiQ61024

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031766; ENSMUSP00000031766; ENSMUSG00000029752
ENSMUST00000115542; ENSMUSP00000111204; ENSMUSG00000029752
GeneIDi27053
KEGGimmu:27053
UCSCiuc009axf.1 mouse

Organism-specific databases

CTDi440
MGIiMGI:1350929 Asns

Phylogenomic databases

eggNOGiKOG0571 Eukaryota
COG0367 LUCA
GeneTreeiENSGT00390000001994
HOGENOMiHOG000027493
HOVERGENiHBG003103
InParanoidiQ61024
KOiK01953
OMAiLFGYKYT
OrthoDBiEOG091G13BD
PhylomeDBiQ61024
TreeFamiTF300603

Enzyme and pathway databases

UniPathwayi
UPA00134;UER00195

ReactomeiR-MMU-70614 Amino acid synthesis and interconversion (transamination)

Miscellaneous databases

PROiPR:Q61024
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029752 Expressed in 303 organ(s), highest expression level in saliva-secreting gland
CleanExiMM_ASNS
ExpressionAtlasiQ61024 baseline and differential
GenevisibleiQ61024 MM

Family and domain databases

CDDicd01991 Asn_Synthase_B_C, 1 hit
cd00712 AsnB, 1 hit
Gene3Di3.40.50.620, 1 hit
3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR006426 Asn_synth_AEB
IPR001962 Asn_synthase
IPR033738 AsnB_N
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00733 Asn_synthase, 1 hit
PF13537 GATase_7, 1 hit
PIRSFiPIRSF001589 Asn_synthetase_glu-h, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01536 asn_synth_AEB, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiASNS_MOUSE
AccessioniPrimary (citable) accession number: Q61024
Secondary accession number(s): Q3TJA1, Q8BPC8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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