UniProtKB - Q60HH4 (ASAH1_MACFA)
Acid ceramidase
ASAH1
Functioni
Lysosomal ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at acidic pH (By similarity).
Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (By similarity).
Has a higher catalytic efficiency towards C12-ceramides versus other ceramides (By similarity).
Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (By similarity).
For the reverse synthetic reaction, the natural sphingosine D-erythro isomer is more efficiently utilized as a substrate compared to D-erythro-dihydrosphingosine and D-erythro-phytosphingosine, while the fatty acids with chain lengths of 12 or 14 carbons are the most efficiently used (By similarity).
Has also an N-acylethanolamine hydrolase activity (By similarity).
By regulating the levels of ceramides, sphingosine and sphingosine-1-phosphate in the epidermis, mediates the calcium-induced differentiation of epidermal keratinocytes (By similarity).
Also indirectly regulates tumor necrosis factor/TNF-induced apoptosis (By similarity).
By regulating the intracellular balance between ceramides and sphingosine, in adrenocortical cells, probably also acts as a regulator of steroidogenesis (By similarity).
By similarityCatalytic activityi
- EC:3.5.1.23By similarity
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
: sphingolipid metabolism Pathwayi
This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 143 | NucleophileBy similarity | 1 | |
Sitei | 162 | Important for catalytic activityBy similarity | 1 | |
Sitei | 320 | Important for catalytic activityBy similarity | 1 | |
Sitei | 333 | Important for catalytic activityBy similarity | 1 |
GO - Molecular functioni
- ceramidase activity Source: UniProtKB-EC
- fatty acid amide hydrolase activity Source: InterPro
- N-acylsphingosine amidohydrolase activity Source: UniProtKB
GO - Biological processi
- cellular response to tumor necrosis factor Source: UniProtKB
- ceramide biosynthetic process Source: UniProtKB
- ceramide catabolic process Source: UniProtKB
- fatty acid metabolic process Source: InterPro
- keratinocyte differentiation Source: UniProtKB
- regulation of programmed necrotic cell death Source: UniProtKB
- regulation of steroid biosynthetic process Source: UniProtKB
- sphingosine biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid metabolism, Sphingolipid metabolism |
Enzyme and pathway databases
UniPathwayi | UPA00222 |
Protein family/group databases
MEROPSi | C89.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Acid ceramidaseCurated (EC:3.5.1.23By similarity)Short name: AC Short name: ACDase Short name: Acid CDase Alternative name(s): Acylsphingosine deacylase N-acylethanolamine hydrolase ASAH1By similarity (EC:3.5.1.-By similarity) N-acylsphingosine amidohydrolase Cleaved into the following 2 chains: Acid ceramidase subunit alphaBy similarity Acid ceramidase subunit betaBy similarity |
Gene namesi | Name:ASAH1By similarity ORF Names:QccE-14484 |
Organismi | Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) |
Taxonomic identifieri | 9541 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSMFAG00000039553 |
Subcellular locationi
Extracellular region or secreted
- extracellular space Source: UniProtKB
Lysosome
- lysosome Source: UniProtKB
Keywords - Cellular componenti
Lysosome, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000002314 | 22 – 142 | Acid ceramidase subunit alphaBy similarityAdd BLAST | 121 | |
ChainiPRO_0000002315 | 143 – 395 | Acid ceramidase subunit betaBy similarityAdd BLAST | 253 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 31 ↔ 340 | Interchain (between alpha and beta subunits)By similarity | ||
Glycosylationi | 173 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 195 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 259 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 286 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 342 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 348 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 388 ↔ 392 | By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
PRIDEi | Q60HH4 |
Interactioni
Subunit structurei
Heterodimer; disulfide-linked. The heterodimer is composed of the disulfide-linked alpha and beta chains produced by autocatalytic cleavage of the precursor.
By similarityProtein-protein interaction databases
STRINGi | 9541.XP_005562749.1 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QVBG, Eukaryota |
Family and domain databases
InterProi | View protein in InterPro IPR016699, Acid_ceramidase-like IPR029130, Acid_ceramidase_N IPR029132, CBAH/NAAA_C |
Pfami | View protein in Pfam PF02275, CBAH, 1 hit PF15508, NAAA-beta, 1 hit |
PIRSFi | PIRSF017632, Acid_ceramidase-like, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MLGRSRLALV LLAAAVSCAV AQHAPPWTED CRKSTYPPSG PTYRGPAPWY
60 70 80 90 100
TINLDLPPYK RWHELMVDKA PMLKVIVNSL KNMVNTFVPS GKVMQIVDEK
110 120 130 140 150
LPGLLGNFPG PFEEEMKGIA AVTDIPLGEI ISYNIFYEFF TLCTSIVAED
160 170 180 190 200
KKGHLIHGRN MDFGVFLGWN INNDTWVITE ELKPLTVNLD FQRNNKTVFK
210 220 230 240 250
ASSFAGYVGM LTGFKPGLFS LTLNERFSVN GGYLGILEWI LGKKDAMWIG
260 270 280 290 300
FLTRTVLENS TSYEEAKNIL TKTKILAPAY FILGGNQSGE GCVITRDRKE
310 320 330 340 350
SLDVYELDAK QGRWYVVQTN YDRWKNPFFL DDRRTPAKMC LNRTTQENIS
360 370 380 390
FENMYDVLST KPVLNKLTVF TTLIDVTKDQ FETYMRDCPD PCIGW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB125153 mRNA Translation: BAD51941.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB125153 mRNA Translation: BAD51941.1 |
3D structure databases
SMRi | Q60HH4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9541.XP_005562749.1 |
Protein family/group databases
MEROPSi | C89.001 |
Proteomic databases
PRIDEi | Q60HH4 |
Organism-specific databases
VEuPathDBi | HostDB:ENSMFAG00000039553 |
Phylogenomic databases
eggNOGi | ENOG502QVBG, Eukaryota |
Enzyme and pathway databases
UniPathwayi | UPA00222 |
Family and domain databases
InterProi | View protein in InterPro IPR016699, Acid_ceramidase-like IPR029130, Acid_ceramidase_N IPR029132, CBAH/NAAA_C |
Pfami | View protein in Pfam PF02275, CBAH, 1 hit PF15508, NAAA-beta, 1 hit |
PIRSFi | PIRSF017632, Acid_ceramidase-like, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ASAH1_MACFA | |
Accessioni | Q60HH4Primary (citable) accession number: Q60HH4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 12, 2005 |
Last sequence update: | November 23, 2004 | |
Last modified: | September 29, 2021 | |
This is version 56 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families