The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - Q60BX0 (GLO2_METCA)
Protein
Hydroxyacylglutathione hydrolase
Gene
gloB
Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Functioni
Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.
UniRule annotationCatalytic activityi
- an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H+UniRule annotationEC:3.1.2.6UniRule annotation
Cofactori
Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation
: methylglyoxal degradation Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.UniRule annotation This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 55 | Zinc 1UniRule annotation | 1 | |
Metal bindingi | 57 | Zinc 1UniRule annotation | 1 | |
Metal bindingi | 59 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 60 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 113 | Zinc 1UniRule annotation | 1 | |
Metal bindingi | 132 | Zinc 1UniRule annotation | 1 | |
Metal bindingi | 132 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 170 | Zinc 2UniRule annotation | 1 |
GO - Molecular functioni
- beta-lactamase activity Source: InterPro
- hydroxyacylglutathione hydrolase activity Source: UniProtKB-UniRule
- zinc ion binding Source: InterPro
GO - Biological processi
- antibiotic catabolic process Source: InterPro
- methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: InterPro
Keywordsi
Molecular function | Hydrolase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00619;UER00676 |
Names & Taxonomyi
Protein namesi | Recommended name: Hydroxyacylglutathione hydrolaseUniRule annotation (EC:3.1.2.6UniRule annotation)Alternative name(s): Glyoxalase IIUniRule annotation Short name: Glx IIUniRule annotation |
Gene namesi | Name:gloBUniRule annotation Ordered Locus Names:MCA0343 |
Organismi | Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) |
Taxonomic identifieri | 243233 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylococcus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000309661 | 1 – 256 | Hydroxyacylglutathione hydrolaseAdd BLAST | 256 |
Interactioni
Subunit structurei
Monomer.
UniRule annotationProtein-protein interaction databases
STRINGi | 243233.MCA0343 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0491, Bacteria |
HOGENOMi | CLU_030571_4_1_6 |
OMAi | CKERARF |
OrthoDBi | 733552at2 |
Family and domain databases
CDDi | cd07723, hydroxyacylglutathione_hydrolase_MBL-fold, 1 hit |
Gene3Di | 3.60.15.10, 1 hit |
HAMAPi | MF_01374, Glyoxalase_2, 1 hit |
InterProi | View protein in InterPro IPR001018, Beta-lactamase_class-B_CS IPR035680, Clx_II_MBL IPR032282, HAGH_C IPR017782, Hydroxyacylglutathione_Hdrlase IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro |
Pfami | View protein in Pfam PF16123, HAGH_C, 1 hit PF00753, Lactamase_B, 1 hit |
PIRSFi | PIRSF005457, Glx, 1 hit |
SMARTi | View protein in SMART SM00849, Lactamase_B, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR03413, GSH_gloB, 1 hit |
i Sequence
Sequence statusi: Complete.
Q60BX0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLEILQIPVL EDNYVYLLHE PGSGATAAVD PAVAGPVLEA LDARGWRLGH
60 70 80 90 100
VLNTHHHGDH VGGNLELKAA TGCTVVGAAG DRHRIPGIDV ALKDGEEFRL
110 120 130 140 150
GSASARMLDV PGHTSGHVAF WFEDDAALFC GDTLFALGCG RLFEGSAEQM
160 170 180 190 200
WRSLERLRAL PAETKVFCAH EYTQANARFA VTIEPGNAAL RERVERVEAL
210 220 230 240 250
RREGAATVPS ILSEELATNP FLRPESPEIR ARLGLPGVPE VEVFAEIRRR
KDVFRG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017282 Genomic DNA Translation: AAU90561.1 |
RefSeqi | WP_010959704.1, NC_002977.6 |
Genome annotation databases
EnsemblBacteriai | AAU90561; AAU90561; MCA0343 |
KEGGi | mca:MCA0343 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017282 Genomic DNA Translation: AAU90561.1 |
RefSeqi | WP_010959704.1, NC_002977.6 |
3D structure databases
AlphaFoldDBi | Q60BX0 |
SMRi | Q60BX0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 243233.MCA0343 |
Genome annotation databases
EnsemblBacteriai | AAU90561; AAU90561; MCA0343 |
KEGGi | mca:MCA0343 |
Phylogenomic databases
eggNOGi | COG0491, Bacteria |
HOGENOMi | CLU_030571_4_1_6 |
OMAi | CKERARF |
OrthoDBi | 733552at2 |
Enzyme and pathway databases
UniPathwayi | UPA00619;UER00676 |
Family and domain databases
CDDi | cd07723, hydroxyacylglutathione_hydrolase_MBL-fold, 1 hit |
Gene3Di | 3.60.15.10, 1 hit |
HAMAPi | MF_01374, Glyoxalase_2, 1 hit |
InterProi | View protein in InterPro IPR001018, Beta-lactamase_class-B_CS IPR035680, Clx_II_MBL IPR032282, HAGH_C IPR017782, Hydroxyacylglutathione_Hdrlase IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro |
Pfami | View protein in Pfam PF16123, HAGH_C, 1 hit PF00753, Lactamase_B, 1 hit |
PIRSFi | PIRSF005457, Glx, 1 hit |
SMARTi | View protein in SMART SM00849, Lactamase_B, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR03413, GSH_gloB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GLO2_METCA | |
Accessioni | Q60BX0Primary (citable) accession number: Q60BX0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 13, 2007 |
Last sequence update: | November 23, 2004 | |
Last modified: | May 25, 2022 | |
This is version 93 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families