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UniProtKB - Q60980 (KLF3_MOUSE)
Protein
Krueppel-like factor 3
Gene
Klf3
Organism
Mus musculus (Mouse)
Status
Functioni
Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis.
2 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 259 – 283 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 289 – 313 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 319 – 341 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- cellular response to peptide Source: Ensembl
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Krueppel-like factor 3Alternative name(s): Basic krueppel-like factor CACCC-box-binding protein BKLF TEF-2 |
Gene namesi | Name:Klf3 Synonyms:Bklf |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1342773, Klf3 |
VEuPathDBi | HostDB:ENSMUSG00000029178 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- nucleoplasm Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 10 | K → A: Reduced sumoylation levels. No effect on DNA-binding and slight reduction of transcriptional repression. Abolishes sumoylation. No effect on DNA-binding but great reduction in transcriptional repression; when associated with A-197. 1 Publication | 1 | |
Mutagenesisi | 12 | E → A: Slight reduction of transcriptional repression. Great reduction of transcriptional repression; when associated with A-199. 1 Publication | 1 | |
Mutagenesisi | 197 | K → A: Reduced sumoylation levels. No effect on DNA-binding and slight reduction of transcriptional repression. Abolishes sumoylation. No effect on DNA-binding but great reduction of transcriptional repression; when associated with A-10. 1 Publication | 1 | |
Mutagenesisi | 199 | E → A: Slight reduction of transcriptional repression. Great reduction of transcriptional repression; when associated with A-199. 1 Publication | 1 | |
Mutagenesisi | 341 | H → A, D, E, N, Q or R: Little change in DNA-binding ability. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047166 | 1 – 344 | Krueppel-like factor 3Add BLAST | 344 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 10 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication | ||
Cross-linki | 68 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 71 | PhosphoserineBy similarity | 1 | |
Modified residuei | 91 | PhosphoserineCombined sources | 1 | |
Modified residuei | 100 | PhosphoserineCombined sources | 1 | |
Modified residuei | 107 | PhosphoserineCombined sources | 1 | |
Modified residuei | 110 | PhosphoserineCombined sources | 1 | |
Cross-linki | 195 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 197 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate | ||
Cross-linki | 197 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 215 | PhosphoserineCombined sources | 1 | |
Modified residuei | 223 | PhosphoserineCombined sources | 1 | |
Modified residuei | 249 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Sumoylated with SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-197 is the major site.1 Publication
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q60980 |
MaxQBi | Q60980 |
PaxDbi | Q60980 |
PRIDEi | Q60980 |
ProteomicsDBi | 269223 |
PTM databases
iPTMneti | Q60980 |
PhosphoSitePlusi | Q60980 |
Expressioni
Tissue specificityi
In 8.5 day embryos, expressed in midbrain, anterior hindbrain and ventral forebrain. In 9 day embryos, expressed throughout ventral anterior half of embryo including midbrain-hindbrain junction, ventral midbrain, diencephalon and forebrain. At 10.5 days, distribution is more widespread with expression also found in developing limb buds. Widely expressed in the adult.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000029178, Expressed in ear and 318 other tissues |
Genevisiblei | Q60980, MM |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 200965, 3 interactors |
STRINGi | 10090.ENSMUSP00000129363 |
Miscellaneous databases
RNActi | Q60980, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q60980 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q60980 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 74 | Repressor domainAdd BLAST | 74 | |
Regioni | 66 – 111 | DisorderedSequence analysisAdd BLAST | 46 | |
Regioni | 235 – 254 | DisorderedSequence analysisAdd BLAST | 20 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 60 – 68 | 9aaTAD; inactiveBy similarity | 9 | |
Motifi | 61 – 65 | CTBP-binding motif | 5 |
Domaini
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. In KLF3, the motif is inactive.By similarity
Sequence similaritiesi
Belongs to the krueppel C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 259 – 283 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 289 – 313 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 319 – 341 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000157456 |
HOGENOMi | CLU_002678_33_0_1 |
InParanoidi | Q60980 |
OMAi | NYMDQMK |
OrthoDBi | 1014205at2759 |
PhylomeDBi | Q60980 |
TreeFami | TF350556 |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q60980-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLMFDPVPVK QEAMDPVSVS FPSNYIESMK PNKYGVIYST PLPDKFFQTP
60 70 80 90 100
EGLTHGIQVE PVDLTVNKRG SPPAAGGSPS SLKFPSHRRA SPGLSMPSSS
110 120 130 140 150
PPIKKYSPPS PGVQPFGVPL SMPPVMAAAL SRHGIRSPGI LPVIQPVVVQ
160 170 180 190 200
PVPFMYTSHL QQPLMVSLSE EMDNSNSGMP VPVIESYEKP LLQKKIKIEP
210 220 230 240 250
GIEPQRTDYY PEEMSPPLMN PVSPPQALLQ ENHPSVIVQP GKRPLPVESP
260 270 280 290 300
DTQRKRRIHR CDYDGCNKVY TKSSHLKAHR RTHTGEKPYK CTWEGCTWKF
310 320 330 340
ARSDELTRHF RKHTGIKPFQ CPDCDRSFSR SDHLALHRKR HMLV
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JDH4 | A0A0G2JDH4_MOUSE | Krueppel-like factor 3 | Klf3 | 142 | Annotation score: | ||
A0A0G2JE81 | A0A0G2JE81_MOUSE | Krueppel-like factor 3 | Klf3 | 55 | Annotation score: | ||
A0A0G2JE66 | A0A0G2JE66_MOUSE | Krueppel-like factor 3 | Klf3 | 138 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U36340 mRNA Translation: AAA93256.1 |
CCDSi | CCDS19301.1 |
PIRi | JC6100 |
RefSeqi | NP_032479.1, NM_008453.5 XP_006503814.1, XM_006503751.3 |
Genome annotation databases
Ensembli | ENSMUST00000165536; ENSMUSP00000129363; ENSMUSG00000029178 ENSMUST00000166409; ENSMUSP00000128429; ENSMUSG00000029178 |
GeneIDi | 16599 |
KEGGi | mmu:16599 |
UCSCi | uc008xmr.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U36340 mRNA Translation: AAA93256.1 |
CCDSi | CCDS19301.1 |
PIRi | JC6100 |
RefSeqi | NP_032479.1, NM_008453.5 XP_006503814.1, XM_006503751.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1P7A | NMR | - | A | 314-344 | [»] | |
1U85 | NMR | - | A | 314-344 | [»] | |
1U86 | NMR | - | A | 314-344 | [»] | |
SMRi | Q60980 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 200965, 3 interactors |
STRINGi | 10090.ENSMUSP00000129363 |
PTM databases
iPTMneti | Q60980 |
PhosphoSitePlusi | Q60980 |
Proteomic databases
jPOSTi | Q60980 |
MaxQBi | Q60980 |
PaxDbi | Q60980 |
PRIDEi | Q60980 |
ProteomicsDBi | 269223 |
Protocols and materials databases
Antibodypediai | 10444, 186 antibodies from 29 providers |
DNASUi | 16599 |
Genome annotation databases
Ensembli | ENSMUST00000165536; ENSMUSP00000129363; ENSMUSG00000029178 ENSMUST00000166409; ENSMUSP00000128429; ENSMUSG00000029178 |
GeneIDi | 16599 |
KEGGi | mmu:16599 |
UCSCi | uc008xmr.1, mouse |
Organism-specific databases
CTDi | 51274 |
MGIi | MGI:1342773, Klf3 |
VEuPathDBi | HostDB:ENSMUSG00000029178 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000157456 |
HOGENOMi | CLU_002678_33_0_1 |
InParanoidi | Q60980 |
OMAi | NYMDQMK |
OrthoDBi | 1014205at2759 |
PhylomeDBi | Q60980 |
TreeFami | TF350556 |
Miscellaneous databases
BioGRID-ORCSi | 16599, 6 hits in 65 CRISPR screens |
ChiTaRSi | Klf3, mouse |
EvolutionaryTracei | Q60980 |
PROi | PR:Q60980 |
RNActi | Q60980, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029178, Expressed in ear and 318 other tissues |
Genevisiblei | Q60980, MM |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | KLF3_MOUSE | |
Accessioni | Q60980Primary (citable) accession number: Q60980 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 177 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families