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Protein

Krueppel-like factor 3

Gene

Klf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri259 – 283C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri289 – 313C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri319 – 341C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

  • DNA binding Source: MGI
  • DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
  • DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Krueppel-like factor 3
Alternative name(s):
Basic krueppel-like factor
CACCC-box-binding protein BKLF
TEF-2
Gene namesi
Name:Klf3
Synonyms:Bklf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1342773 Klf3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10K → A: Reduced sumoylation levels. No effect on DNA-binding and slight reduction of transcriptional repression. Abolishes sumoylation. No effect on DNA-binding but great reduction in transcriptional repression; when associated with A-197. 1 Publication1
Mutagenesisi12E → A: Slight reduction of transcriptional repression. Great reduction of transcriptional repression; when associated with A-199. 1 Publication1
Mutagenesisi197K → A: Reduced sumoylation levels. No effect on DNA-binding and slight reduction of transcriptional repression. Abolishes sumoylation. No effect on DNA-binding but great reduction of transcriptional repression; when associated with A-10. 1 Publication1
Mutagenesisi199E → A: Slight reduction of transcriptional repression. Great reduction of transcriptional repression; when associated with A-199. 1 Publication1
Mutagenesisi341H → A, D, E, N, Q or R: Little change in DNA-binding ability. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000471661 – 344Krueppel-like factor 3Add BLAST344

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki10Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication
Cross-linki68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei71PhosphoserineBy similarity1
Modified residuei91PhosphoserineCombined sources1
Modified residuei100PhosphoserineCombined sources1
Modified residuei107PhosphoserineCombined sources1
Modified residuei110PhosphoserineCombined sources1
Cross-linki195Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki197Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate
Cross-linki197Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei215PhosphoserineCombined sources1
Modified residuei223PhosphoserineCombined sources1
Modified residuei249PhosphoserineBy similarity1

Post-translational modificationi

Sumoylated with SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-197 is the major site.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ60980
PaxDbiQ60980
PRIDEiQ60980

PTM databases

iPTMnetiQ60980
PhosphoSitePlusiQ60980

Expressioni

Tissue specificityi

In 8.5 day embryos, expressed in midbrain, anterior hindbrain and ventral forebrain. In 9 day embryos, expressed throughout ventral anterior half of embryo including midbrain-hindbrain junction, ventral midbrain, diencephalon and forebrain. At 10.5 days, distribution is more widespread with expression also found in developing limb buds. Widely expressed in the adult.1 Publication

Gene expression databases

BgeeiENSMUSG00000029178 Expressed in 297 organ(s), highest expression level in ear
CleanExiMM_KLF3
ExpressionAtlasiQ60980 baseline and differential
GenevisibleiQ60980 MM

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi200965, 2 interactors
STRINGi10090.ENSMUSP00000128429

Structurei

Secondary structure

1344
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ60980
SMRiQ60980
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60980

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 74Repressor domainAdd BLAST74

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi61 – 65CTBP-binding motif5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 250Pro-richAdd BLAST250

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri259 – 283C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri289 – 313C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri319 – 341C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00760000118998
HOGENOMiHOG000232138
HOVERGENiHBG003941
InParanoidiQ60980
KOiK15605
OrthoDBiEOG091G1BN0
PhylomeDBiQ60980
TreeFamiTF350556

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q60980-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLMFDPVPVK QEAMDPVSVS FPSNYIESMK PNKYGVIYST PLPDKFFQTP
60 70 80 90 100
EGLTHGIQVE PVDLTVNKRG SPPAAGGSPS SLKFPSHRRA SPGLSMPSSS
110 120 130 140 150
PPIKKYSPPS PGVQPFGVPL SMPPVMAAAL SRHGIRSPGI LPVIQPVVVQ
160 170 180 190 200
PVPFMYTSHL QQPLMVSLSE EMDNSNSGMP VPVIESYEKP LLQKKIKIEP
210 220 230 240 250
GIEPQRTDYY PEEMSPPLMN PVSPPQALLQ ENHPSVIVQP GKRPLPVESP
260 270 280 290 300
DTQRKRRIHR CDYDGCNKVY TKSSHLKAHR RTHTGEKPYK CTWEGCTWKF
310 320 330 340
ARSDELTRHF RKHTGIKPFQ CPDCDRSFSR SDHLALHRKR HMLV
Length:344
Mass (Da):38,561
Last modified:November 1, 1996 - v1
Checksum:i2BB7E3B63A7C1D88
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JE66A0A0G2JE66_MOUSE
Krueppel-like factor 3
Klf3
138Annotation score:
A0A0G2JE81A0A0G2JE81_MOUSE
Krueppel-like factor 3
Klf3
55Annotation score:
A0A0G2JDH4A0A0G2JDH4_MOUSE
Krueppel-like factor 3
Klf3
142Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36340 mRNA Translation: AAA93256.1
CCDSiCCDS19301.1
PIRiJC6100
RefSeqiNP_032479.1, NM_008453.5
XP_006503814.1, XM_006503751.3
UniGeneiMm.392759
Mm.439720

Genome annotation databases

EnsembliENSMUST00000165536; ENSMUSP00000129363; ENSMUSG00000029178
ENSMUST00000166409; ENSMUSP00000128429; ENSMUSG00000029178
GeneIDi16599
KEGGimmu:16599
UCSCiuc008xmr.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36340 mRNA Translation: AAA93256.1
CCDSiCCDS19301.1
PIRiJC6100
RefSeqiNP_032479.1, NM_008453.5
XP_006503814.1, XM_006503751.3
UniGeneiMm.392759
Mm.439720

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1P7ANMR-A314-344[»]
1U85NMR-A314-344[»]
1U86NMR-A314-344[»]
ProteinModelPortaliQ60980
SMRiQ60980
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200965, 2 interactors
STRINGi10090.ENSMUSP00000128429

PTM databases

iPTMnetiQ60980
PhosphoSitePlusiQ60980

Proteomic databases

MaxQBiQ60980
PaxDbiQ60980
PRIDEiQ60980

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000165536; ENSMUSP00000129363; ENSMUSG00000029178
ENSMUST00000166409; ENSMUSP00000128429; ENSMUSG00000029178
GeneIDi16599
KEGGimmu:16599
UCSCiuc008xmr.1 mouse

Organism-specific databases

CTDi51274
MGIiMGI:1342773 Klf3

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00760000118998
HOGENOMiHOG000232138
HOVERGENiHBG003941
InParanoidiQ60980
KOiK15605
OrthoDBiEOG091G1BN0
PhylomeDBiQ60980
TreeFamiTF350556

Miscellaneous databases

ChiTaRSiKlf3 mouse
EvolutionaryTraceiQ60980
PROiPR:Q60980
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029178 Expressed in 297 organ(s), highest expression level in ear
CleanExiMM_KLF3
ExpressionAtlasiQ60980 baseline and differential
GenevisibleiQ60980 MM

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiKLF3_MOUSE
AccessioniPrimary (citable) accession number: Q60980
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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