UniProtKB - Q60972 (RBBP4_MOUSE)
Histone-binding protein RBBP4
Rbbp4
Functioni
GO - Molecular functioni
- DNA-dependent ATPase activity Source: Ensembl
- histone deacetylase binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- chromatin assembly Source: UniProtKB
- chromatin remodeling Source: HGNC-UCL
- DNA replication Source: UniProtKB-KW
- DNA replication-dependent nucleosome assembly Source: MGI
- DNA replication-independent nucleosome assembly Source: MGI
Keywordsi
Molecular function | Chromatin regulator, Repressor |
Biological process | Cell cycle, DNA replication, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-1538133, G0 and Early G1 R-MMU-212300, PRC2 methylates histones and DNA R-MMU-2559580, Oxidative Stress Induced Senescence R-MMU-3214815, HDACs deacetylate histones R-MMU-3214841, PKMTs methylate histone lysines R-MMU-606279, Deposition of new CENPA-containing nucleosomes at the centromere R-MMU-6804758, Regulation of TP53 Activity through Acetylation R-MMU-73762, RNA Polymerase I Transcription Initiation R-MMU-8943724, Regulation of PTEN gene transcription R-MMU-8953750, Transcriptional Regulation by E2F6 |
Names & Taxonomyi
Protein namesi | Recommended name: Histone-binding protein RBBP4Alternative name(s): Chromatin assembly factor 1 subunit C Short name: CAF-1 subunit C Chromatin assembly factor I p48 subunit Short name: CAF-I 48 kDa subunit Short name: CAF-I p48 Nucleosome-remodeling factor subunit RBAP48 Retinoblastoma-binding protein 4 Short name: RBBP-4 Retinoblastoma-binding protein p48 |
Gene namesi | Name:Rbbp4 Synonyms:Rbap48 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1194912, Rbbp4 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Note: Localizes to chromatin as part of the PRC2 complex.By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- ESC/E(Z) complex Source: UniProtKB
- nuclear chromatin Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
- NuRD complex Source: MGI
- NURF complex Source: MGI
Other locations
- CAF-1 complex Source: UniProtKB
- protein-containing complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000051187 | 2 – 425 | Histone-binding protein RBBP4Add BLAST | 424 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 4 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 4 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Cross-linki | 4 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity | ||
Modified residuei | 110 | PhosphoserineBy similarity | 1 | |
Modified residuei | 160 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 160 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 355 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q60972 |
jPOSTi | Q60972 |
MaxQBi | Q60972 |
PaxDbi | Q60972 |
PeptideAtlasi | Q60972 |
PRIDEi | Q60972 |
2D gel databases
REPRODUCTION-2DPAGEi | Q60972 |
PTM databases
iPTMneti | Q60972 |
PhosphoSitePlusi | Q60972 |
SwissPalmi | Q60972 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000057236, Expressed in cumulus cell and 315 other tissues |
ExpressionAtlasi | Q60972, baseline and differential |
Genevisiblei | Q60972, MM |
Interactioni
Subunit structurei
Binds directly to helix 1 of the histone fold of histone H4, a region that is not accessible when H4 is in chromatin. Subunit of the chromatin assembly factor 1 (CAF-1) complex, which is composed of RBBP4, CHAF1B and CHAF1A (By similarity). Subunit of the core histone deacetylase (HDAC) complex, which is composed of HDAC1, HDAC2, RBBP4 and RBBP7 (By similarity). The core HDAC complex associates with SIN3A, ARID4B/SAP180, SAP18, SAP30, SAP130, SUDS3/SAP45 and possibly ARID4A/RBP1 and ING1 to form the SIN3 HDAC complex (Probable). The core HDAC complex may also associate with MTA2, MBD3, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylase complex (the NuRD complex) (By similarity). The NuRD complex may also interact with MBD3L1 and MBD3L2 (By similarity).
Interacts with MTA1 (By similarity).
Component of the PRC2 complex, which consists of the core subunits EED, EZH1 or EZH2, SUZ12, and RBBP4, and various combinations of accessory subunits including AEBP2, JARID2, PHF19, MTF2 and EPOP (PubMed:19026780).
Forms a monomeric PRC2.2 (class 2) complex consisting of at least SUZ12, RBBP4, AEBP2 and JARID2 (By similarity).
Forms a dimeric PRC2.1 (class 1, PRC-PCL) complex consisting of at least SUZ12, RBBP4, and PHF19; PHF19 stabilizes the dimeric structure which enhances PRC2 interaction with chromatin (By similarity). Part of the nucleosome remodeling factor (NURF) complex which consists of SMARCA1; BPTF; RBBP4 and RBBP7 (PubMed:10866654).
Interacts with SUV39H1 and HDAC7 (PubMed:10984530, PubMed:11788710).
Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1) (By similarity).
Interacts with SPEN/MINT (By similarity).
Interacts with BRCA1 (By similarity).
Interacts with CREBBP, and this interaction may be enhanced by the binding of phosphorylated CREB1 to CREBBP (By similarity).
Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2 (By similarity). The complex exists in quiescent cells where it represses cell cycle-dependent genes (By similarity). It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2 (By similarity).
Interacts with PHF6 (By similarity).
Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1 (PubMed:28554894).
Interacts with ERCC6 (By similarity).
By similarity1 Publication5 PublicationsGO - Molecular functioni
- histone deacetylase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202816, 40 interactors |
ComplexPortali | CPX-3441, SIN3A histone deacetylase complex, ES cell-specific variant CPX-3443, SIN3A histone deacetylase complex CPX-3444, SIN3B histone deacetylase complex CPX-570, Chromatin assembly factor 1 complex CPX-694, NuRF chromatin remodelling complex CPX-953, MBD2/NuRD nucleosome remodeling and deacetylase complex CPX-954, MBD3/NuRD nucleosome remodeling and deacetylase complex |
CORUMi | Q60972 |
DIPi | DIP-32855N |
IntActi | Q60972, 20 interactors |
MINTi | Q60972 |
STRINGi | 10090.ENSMUSP00000099658 |
Miscellaneous databases
RNActi | Q60972, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 122 – 155 | WD 1Add BLAST | 34 | |
Repeati | 175 – 206 | WD 2Add BLAST | 32 | |
Repeati | 225 – 256 | WD 3Add BLAST | 32 | |
Repeati | 271 – 302 | WD 4Add BLAST | 32 | |
Repeati | 315 – 346 | WD 5Add BLAST | 32 | |
Repeati | 372 – 403 | WD 6Add BLAST | 32 |
Sequence similaritiesi
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0264, Eukaryota |
GeneTreei | ENSGT00940000153375 |
HOGENOMi | CLU_020445_3_1_1 |
InParanoidi | Q60972 |
OMAi | HLADFSW |
OrthoDBi | 831322at2759 |
TreeFami | TF106485 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR022052, Histone-bd_RBBP4_N IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF12265, CAF1C_H4-bd, 1 hit PF00400, WD40, 5 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 6 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 3 hits PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MADKEAAFDD AVEERVINEE YKIWKKNTPF LYDLVMTHAL EWPSLTAQWL
60 70 80 90 100
PDVTRPEGKD FSIHRLVLGT HTSDEQNHLV IASVQLPNDD AQFDASHYDS
110 120 130 140 150
EKGEFGGFGS VSGKIEIEIK INHEGEVNRA RYMPQNPCII ATKTPSSDVL
160 170 180 190 200
VFDYTKHPSK PDPSGECNPD LRLRGHQKEG YGLSWNPNLS GHLLSASDDH
210 220 230 240 250
TICLWDISAV PKEGKVVDAK TIFTGHTAVV EDVSWHLLHE SLFGSVADDQ
260 270 280 290 300
KLMIWDTRSN NTSKPSHSVD AHTAEVNCLS FNPYSEFILA TGSADKTVAL
310 320 330 340 350
WDLRNLKLKL HSFESHKDEI FQVQWSPHNE TILASSGTDR RLNVWDLSKI
360 370 380 390 400
GEEQSPEDAE DGPPELLFIH GGHTAKISDF SWNPNEPWVI CSVSEDNIMQ
410 420
VWQMAENIYN DEDPEGSVDP EGQGS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA8Y5E8 | A8Y5E8_MOUSE | Histone-binding protein RBBP4 | Rbbp4 | 48 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 213 | E → K in AAC52275 (PubMed:7503932).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U35141 mRNA Translation: AAC52275.1 Frameshift. AL607123 Genomic DNA No translation available. CU234133 Genomic DNA No translation available. CH466552 Genomic DNA Translation: EDL30202.1 BC138568 mRNA Translation: AAI38569.1 BC138570 mRNA Translation: AAI38571.1 |
CCDSi | CCDS18688.1 |
PIRi | I49366 |
RefSeqi | NP_033056.2, NM_009030.3 |
Genome annotation databases
Ensembli | ENSMUST00000102598; ENSMUSP00000099658; ENSMUSG00000057236 |
GeneIDi | 19646 |
KEGGi | mmu:19646 |
UCSCi | uc008uwt.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U35141 mRNA Translation: AAC52275.1 Frameshift. AL607123 Genomic DNA No translation available. CU234133 Genomic DNA No translation available. CH466552 Genomic DNA Translation: EDL30202.1 BC138568 mRNA Translation: AAI38569.1 BC138570 mRNA Translation: AAI38571.1 |
CCDSi | CCDS18688.1 |
PIRi | I49366 |
RefSeqi | NP_033056.2, NM_009030.3 |
3D structure databases
SMRi | Q60972 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202816, 40 interactors |
ComplexPortali | CPX-3441, SIN3A histone deacetylase complex, ES cell-specific variant CPX-3443, SIN3A histone deacetylase complex CPX-3444, SIN3B histone deacetylase complex CPX-570, Chromatin assembly factor 1 complex CPX-694, NuRF chromatin remodelling complex CPX-953, MBD2/NuRD nucleosome remodeling and deacetylase complex CPX-954, MBD3/NuRD nucleosome remodeling and deacetylase complex |
CORUMi | Q60972 |
DIPi | DIP-32855N |
IntActi | Q60972, 20 interactors |
MINTi | Q60972 |
STRINGi | 10090.ENSMUSP00000099658 |
PTM databases
iPTMneti | Q60972 |
PhosphoSitePlusi | Q60972 |
SwissPalmi | Q60972 |
2D gel databases
REPRODUCTION-2DPAGEi | Q60972 |
Proteomic databases
EPDi | Q60972 |
jPOSTi | Q60972 |
MaxQBi | Q60972 |
PaxDbi | Q60972 |
PeptideAtlasi | Q60972 |
PRIDEi | Q60972 |
Genome annotation databases
Ensembli | ENSMUST00000102598; ENSMUSP00000099658; ENSMUSG00000057236 |
GeneIDi | 19646 |
KEGGi | mmu:19646 |
UCSCi | uc008uwt.1, mouse |
Organism-specific databases
CTDi | 5928 |
MGIi | MGI:1194912, Rbbp4 |
Phylogenomic databases
eggNOGi | KOG0264, Eukaryota |
GeneTreei | ENSGT00940000153375 |
HOGENOMi | CLU_020445_3_1_1 |
InParanoidi | Q60972 |
OMAi | HLADFSW |
OrthoDBi | 831322at2759 |
TreeFami | TF106485 |
Enzyme and pathway databases
Reactomei | R-MMU-1538133, G0 and Early G1 R-MMU-212300, PRC2 methylates histones and DNA R-MMU-2559580, Oxidative Stress Induced Senescence R-MMU-3214815, HDACs deacetylate histones R-MMU-3214841, PKMTs methylate histone lysines R-MMU-606279, Deposition of new CENPA-containing nucleosomes at the centromere R-MMU-6804758, Regulation of TP53 Activity through Acetylation R-MMU-73762, RNA Polymerase I Transcription Initiation R-MMU-8943724, Regulation of PTEN gene transcription R-MMU-8953750, Transcriptional Regulation by E2F6 |
Miscellaneous databases
BioGRID-ORCSi | 19646, 13 hits in 22 CRISPR screens |
ChiTaRSi | Rbbp4, mouse |
PROi | PR:Q60972 |
RNActi | Q60972, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000057236, Expressed in cumulus cell and 315 other tissues |
ExpressionAtlasi | Q60972, baseline and differential |
Genevisiblei | Q60972, MM |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR022052, Histone-bd_RBBP4_N IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF12265, CAF1C_H4-bd, 1 hit PF00400, WD40, 5 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 6 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 3 hits PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RBBP4_MOUSE | |
Accessioni | Q60972Primary (citable) accession number: Q60972 Secondary accession number(s): A2A875 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 193 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families