UniProtKB - Q60960 (IMA5_MOUSE)
Importin subunit alpha-5
Kpna1
Functioni
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
GO - Molecular functioni
- nuclear import signal receptor activity Source: GO_Central
- nuclear localization sequence binding Source: MGI
GO - Biological processi
- NLS-bearing protein import into nucleus Source: MGI
- postsynapse to nucleus signaling pathway Source: SynGO
- regulation of apoptotic process Source: MGI
- regulation of canonical Wnt signaling pathway Source: MGI
- satellite cell activation involved in skeletal muscle regeneration Source: MGI
- skeletal muscle satellite cell proliferation Source: MGI
- skeletal muscle tissue regeneration Source: MGI
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-140342, Apoptosis induced DNA fragmentation R-MMU-68616, Assembly of the ORC complex at the origin of replication |
Names & Taxonomyi
Protein namesi | Recommended name: Importin subunit alpha-5Alternative name(s): Importin alpha-S1 Karyopherin subunit alpha-1 Nucleoprotein interactor 1 Short name: NPI-1 RAG cohort protein 2 SRP1-beta |
Gene namesi | Name:Kpna1 Synonyms:Rch2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:103560, Kpna1 |
VEuPathDBi | HostDB:ENSMUSG00000022905 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- dendrite Source: UniProtKB
- glutamatergic synapse Source: SynGO
- NLS-dependent protein nuclear import complex Source: Ensembl
- postsynaptic density Source: SynGO
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120720 | 1 – 538 | Importin subunit alpha-5Add BLAST | 538 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 3 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 63 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q60960 |
jPOSTi | Q60960 |
MaxQBi | Q60960 |
PaxDbi | Q60960 |
PeptideAtlasi | Q60960 |
PRIDEi | Q60960 |
ProteomicsDBi | 267240 |
PTM databases
iPTMneti | Q60960 |
PhosphoSitePlusi | Q60960 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000022905, Expressed in cochlea and 334 other tissues |
ExpressionAtlasi | Q60960, baseline and differential |
Genevisiblei | Q60960, MM |
Interactioni
Subunit structurei
Heterodimer; with KPNB1 (By similarity).
Interacts with NSMF; the interaction occurs in a calcium-independent manner after synaptic NMDA receptor stimulation and is required for nuclear import of NSMF but is competed by CABP1 (By similarity).
Interacts with APEX1 (via its N-terminus) (By similarity).
Interacts with CTNNBL1 (via its N-terminal) (By similarity).
Interacts with AICDA (via its NLS) (By similarity).
Interacts with ANP32E (PubMed:10692581).
Interacts with ZIC3 (PubMed:18716025).
Interacts with SNAI1 (via zinc fingers) (By similarity).
Interacts with DCAF8 (By similarity).
Interacts with ITSN1 isoform 2 (PubMed:29599122).
By similarity3 PublicationsBinary interactionsi
Q60960
With | #Exp. | IntAct |
---|---|---|
SF1 [Q15637] from Homo sapiens. | 3 | EBI-8573008,EBI-744603 |
Protein-protein interaction databases
BioGRIDi | 201006, 18 interactors |
ComplexPortali | CPX-1056, Importin complex, KPNA1 variant |
DIPi | DIP-48614N |
IntActi | Q60960, 5 interactors |
MINTi | Q60960 |
STRINGi | 10090.ENSMUSP00000004054 |
Miscellaneous databases
RNActi | Q60960, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 57 | IBBPROSITE-ProRule annotationAdd BLAST | 57 | |
Repeati | 77 – 117 | ARM 1; truncatedAdd BLAST | 41 | |
Repeati | 118 – 161 | ARM 2Add BLAST | 44 | |
Repeati | 162 – 206 | ARM 3Add BLAST | 45 | |
Repeati | 207 – 245 | ARM 4Add BLAST | 39 | |
Repeati | 246 – 290 | ARM 5Add BLAST | 45 | |
Repeati | 291 – 330 | ARM 6Add BLAST | 40 | |
Repeati | 331 – 372 | ARM 7Add BLAST | 42 | |
Repeati | 373 – 412 | ARM 8Add BLAST | 40 | |
Repeati | 413 – 457 | ARM 9Add BLAST | 45 | |
Repeati | 460 – 504 | ARM 10; atypicalAdd BLAST | 45 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 41 | DisorderedSequence analysisAdd BLAST | 41 | |
Regioni | 149 – 241 | NLS binding site (major)By similarityAdd BLAST | 93 | |
Regioni | 245 – 437 | Binding to RAG1By similarityAdd BLAST | 193 | |
Regioni | 318 – 406 | NLS binding site (minor)By similarityAdd BLAST | 89 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 42 – 51 | Nuclear localization signalBy similarity | 10 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 10 – 41 | Basic and acidic residuesSequence analysisAdd BLAST | 32 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0166, Eukaryota |
GeneTreei | ENSGT01030000234650 |
HOGENOMi | CLU_018084_6_0_1 |
InParanoidi | Q60960 |
OMAi | MTEMIFS |
OrthoDBi | 1111872at2759 |
PhylomeDBi | Q60960 |
TreeFami | TF354205 |
Family and domain databases
Gene3Di | 1.20.5.690, 1 hit 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR032413, Arm_3 IPR000225, Armadillo IPR002652, Importin-a_IBB IPR036975, Importin-a_IBB_sf IPR024931, Importin_alpha |
Pfami | View protein in Pfam PF00514, Arm, 8 hits PF16186, Arm_3, 1 hit PF01749, IBB, 1 hit |
PIRSFi | PIRSF005673, Importin_alpha, 1 hit |
SMARTi | View protein in SMART SM00185, ARM, 8 hits |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50176, ARM_REPEAT, 3 hits PS51214, IBB, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSTPGKENFR LKSYKNKSLN PDEMRRRREE EGLQLRKQKR EEQLFKRRNV
60 70 80 90 100
ATAEEETEEE VMSDGGFHEA QINNMEMAPG GVITSDMTDM IFSNSPEQQL
110 120 130 140 150
SATQKFRKLL SKEPNPPIDE VINTPGVVAR FVEFLKRKEN CTLQFESAWV
160 170 180 190 200
LTNIASGNSL QTRNVIQAGA VPIFIELLSS EFEDVQEQAV WALGNIAGDS
210 220 230 240 250
TMCRDYVLNC NILPPLLQLF SKQNRLTMTR NAVWALSNLC RGKSPPPEFA
260 270 280 290 300
KVSPCLNVLS WLLFVSDTDV LADACWALSY LSDGPNDKIQ AVIDAGVCRR
310 320 330 340 350
LVELLMHNDY KVVSPALRAV GNIVTGDDIQ TQVILNCSAL QSLLHLLSSP
360 370 380 390 400
KESIKKEACW TISNITAGNR AQIQTVIDAN MFPALISILQ TAEFRTRKEA
410 420 430 440 450
AWAITNATSG GSAEQIKYLV ELGCIKPLCD LLTVMDAKIV QVALNGLENI
460 470 480 490 500
LRLGEQEAKR NGSGINPYCA LIEEAYGLDK IEFLQSHENQ EIYQKAFDLI
510 520 530
EHYFGTEDED SSIAPQVDLS QQQYIFQQCE APMEGFQL
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3UWJ8 | G3UWJ8_MOUSE | Importin subunit alpha-5 | Kpna1 | 119 | Annotation score: | ||
G3UXT6 | G3UXT6_MOUSE | Importin subunit alpha-5 | Kpna1 | 145 | Annotation score: | ||
G3UZK5 | G3UZK5_MOUSE | Importin subunit alpha-5 | Kpna1 | 182 | Annotation score: | ||
G3UXW7 | G3UXW7_MOUSE | Importin subunit alpha-5 | Kpna1 | 98 | Annotation score: | ||
G3UYD2 | G3UYD2_MOUSE | Importin subunit alpha-5 | Kpna1 | 45 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 83 | I → T in AAC52450 (PubMed:8631802).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U34228 mRNA Translation: AAC52450.1 AK028259 mRNA Translation: BAC25847.1 AK028307 mRNA Translation: BAC25872.1 AK154800 mRNA Translation: BAE32838.1 AK169311 mRNA Translation: BAE41066.1 AK169348 mRNA Translation: BAE41098.1 BC006771 mRNA Translation: AAH06771.1 U20619 mRNA No translation available. |
CCDSi | CCDS28144.1 |
RefSeqi | NP_032491.2, NM_008465.5 XP_006521877.1, XM_006521814.3 |
Genome annotation databases
Ensembli | ENSMUST00000004054; ENSMUSP00000004054; ENSMUSG00000022905 |
GeneIDi | 16646 |
KEGGi | mmu:16646 |
UCSCi | uc007zcb.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U34228 mRNA Translation: AAC52450.1 AK028259 mRNA Translation: BAC25847.1 AK028307 mRNA Translation: BAC25872.1 AK154800 mRNA Translation: BAE32838.1 AK169311 mRNA Translation: BAE41066.1 AK169348 mRNA Translation: BAE41098.1 BC006771 mRNA Translation: AAH06771.1 U20619 mRNA No translation available. |
CCDSi | CCDS28144.1 |
RefSeqi | NP_032491.2, NM_008465.5 XP_006521877.1, XM_006521814.3 |
3D structure databases
SMRi | Q60960 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201006, 18 interactors |
ComplexPortali | CPX-1056, Importin complex, KPNA1 variant |
DIPi | DIP-48614N |
IntActi | Q60960, 5 interactors |
MINTi | Q60960 |
STRINGi | 10090.ENSMUSP00000004054 |
PTM databases
iPTMneti | Q60960 |
PhosphoSitePlusi | Q60960 |
Proteomic databases
EPDi | Q60960 |
jPOSTi | Q60960 |
MaxQBi | Q60960 |
PaxDbi | Q60960 |
PeptideAtlasi | Q60960 |
PRIDEi | Q60960 |
ProteomicsDBi | 267240 |
Protocols and materials databases
Antibodypediai | 32899, 392 antibodies from 39 providers |
DNASUi | 16646 |
Genome annotation databases
Ensembli | ENSMUST00000004054; ENSMUSP00000004054; ENSMUSG00000022905 |
GeneIDi | 16646 |
KEGGi | mmu:16646 |
UCSCi | uc007zcb.1, mouse |
Organism-specific databases
CTDi | 3836 |
MGIi | MGI:103560, Kpna1 |
VEuPathDBi | HostDB:ENSMUSG00000022905 |
Phylogenomic databases
eggNOGi | KOG0166, Eukaryota |
GeneTreei | ENSGT01030000234650 |
HOGENOMi | CLU_018084_6_0_1 |
InParanoidi | Q60960 |
OMAi | MTEMIFS |
OrthoDBi | 1111872at2759 |
PhylomeDBi | Q60960 |
TreeFami | TF354205 |
Enzyme and pathway databases
Reactomei | R-MMU-140342, Apoptosis induced DNA fragmentation R-MMU-68616, Assembly of the ORC complex at the origin of replication |
Miscellaneous databases
BioGRID-ORCSi | 16646, 0 hits in 63 CRISPR screens |
ChiTaRSi | Kpna1, mouse |
PROi | PR:Q60960 |
RNActi | Q60960, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022905, Expressed in cochlea and 334 other tissues |
ExpressionAtlasi | Q60960, baseline and differential |
Genevisiblei | Q60960, MM |
Family and domain databases
Gene3Di | 1.20.5.690, 1 hit 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR032413, Arm_3 IPR000225, Armadillo IPR002652, Importin-a_IBB IPR036975, Importin-a_IBB_sf IPR024931, Importin_alpha |
Pfami | View protein in Pfam PF00514, Arm, 8 hits PF16186, Arm_3, 1 hit PF01749, IBB, 1 hit |
PIRSFi | PIRSF005673, Importin_alpha, 1 hit |
SMARTi | View protein in SMART SM00185, ARM, 8 hits |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50176, ARM_REPEAT, 3 hits PS51214, IBB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IMA5_MOUSE | |
Accessioni | Q60960Primary (citable) accession number: Q60960 Secondary accession number(s): Q3TF32 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 23, 2022 | |
This is version 185 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families