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Protein

Centrosome-associated protein CEP250

Gene

Cep250

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably plays an important role in centrosome cohesion during interphase.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle

Enzyme and pathway databases

ReactomeiR-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-8854518 AURKA Activation by TPX2

Names & Taxonomyi

Protein namesi
Recommended name:
Centrosome-associated protein CEP250
Alternative name(s):
250 kDa centrosomal protein
Short name:
Cep250
Centrosomal Nek2-associated protein 1
Short name:
C-Nap1
Centrosomal protein 2
Intranuclear matrix protein
Gene namesi
Name:Cep250
Synonyms:Cep2, Inmp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:108084 Cep250

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000894881 – 2414Centrosome-associated protein CEP250Add BLAST2414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2292PhosphoserineBy similarity1
Modified residuei2389Phosphoserine; by NEK2By similarity1
Modified residuei2393Phosphoserine; by NEK2By similarity1

Post-translational modificationi

C-terminal part is phosphorylated by NEK2. Dephosphorylated in vitro by the PP1 phosphatase (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ60952
MaxQBiQ60952
PaxDbiQ60952
PeptideAtlasiQ60952
PRIDEiQ60952

PTM databases

iPTMnetiQ60952
PhosphoSitePlusiQ60952

Expressioni

Gene expression databases

BgeeiENSMUSG00000038241 Expressed in 142 organ(s), highest expression level in olfactory epithelium
CleanExiMM_CEP250
ExpressionAtlasiQ60952 baseline and differential
GenevisibleiQ60952 MM

Interactioni

Subunit structurei

Monomer and homodimer (Probable). Forms a complex in vitro with both NEK2 kinase and the PPP1CC catalytic subunit of protein phosphatase 1 (PP1) (By similarity). Interacts with CEP135 (By similarity). Interacts with CROCC/rootletin (PubMed:16339073). Interacts with CNTLN (By similarity). Interacts with NIN (via C-terminus) (PubMed:27565344).By similarityCurated2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200766, 6 interactors
IntActiQ60952, 8 interactors
STRINGi10090.ENSMUSP00000105248

Structurei

3D structure databases

SMRiQ60952
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili91 – 153Sequence analysisAdd BLAST63
Coiled coili248 – 357Sequence analysisAdd BLAST110
Coiled coili400 – 1165Sequence analysisAdd BLAST766
Coiled coili1237 – 2200Sequence analysisAdd BLAST964
Coiled coili2231 – 2290Sequence analysisAdd BLAST60
Coiled coili2320 – 2345Sequence analysisAdd BLAST26

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi43 – 289Glu-richAdd BLAST247

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFAN Eukaryota
ENOG410XPG3 LUCA
GeneTreeiENSGT00730000110800
HOGENOMiHOG000246955
HOVERGENiHBG081320
InParanoidiQ60952
KOiK16464
OMAiRIETSII

Family and domain databases

InterProiView protein in InterPro
IPR026048 CEP250
PANTHERiPTHR23159:SF1 PTHR23159:SF1, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

Q60952-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
METGSPGLNM KPQSLQLVLE GQVLALQQQM AENQAASWRK LKNSQEAQKR
60 70 80 90 100
QATLVRKLQA KVLQYRSWCQ DLEKRLEATG GLIPQRWESV EEPNLEQLLI
110 120 130 140 150
RLEEEQQRCE SLVEVNTELR LHMEKADVVN KALQEDVEKL TVDWSRARDE
160 170 180 190 200
LVRKESQWRM EQEFFKGYLR GEHGRLLNLW REVVTFRRHF LKMKSATDRD
210 220 230 240 250
LTELKAEHAR LSGSLLTCCL RLTLRAQSRE SSGSGRTEES EPARLLLLVA
260 270 280 290 300
KTQALEKEAH EKSQELMQLK SHGDLEKAEL QDRVTELSAL LTQSQKQNED
310 320 330 340 350
YEKMVKALRE TMEILETNHA ELMEHEASLS RNAQEEKLSL QQVIKAITQA
360 370 380 390 400
LASVEEEDTV TQSSGHEDSL QSDCNGLSQF DPQDPDRALT LVQSVLTRRQ
410 420 430 440 450
QAVQDLRQQL SGCQEAMSFL QQQHDQWEEE GRALREKLQK LTGERDALAG
460 470 480 490 500
QTVGLQGEVD SLSRERELLQ KARGELQQQL EVLEQEAWRL RRMNMELQLQ
510 520 530 540 550
GDSAQGEKLE QQEELHLAVR ERERLQETLV GLEAKQSESL SELLTLREAL
560 570 580 590 600
ESSRLEGELL KQERVEVAAA LARAEQSIVE LSGSENSLKA EVADLRAAAV
610 620 630 640 650
KLGALNEALA LDKVELNQQL LQLEQENQSL CSRVEAAEQL RSALQVDLAE
660 670 680 690 700
AERRREALWE KKTQLETQLQ KAEEAGAELQ AELRGTREEK EELKDKLSEA
710 720 730 740 750
HHQQETATAH LEQLHQDAER QEETLARAVQ EKEALVRERA ALEVRLQAVE
760 770 780 790 800
RDRQDLTEHV LGLRSAKEQL ESNLFEAQQQ NSVIQVTKGQ LEVQIQTIIQ
810 820 830 840 850
AKEVIQGEVK CLKLELDAER TRAEQEWDAV ARQLAQAEQE GQASLERQKV
860 870 880 890 900
AHEEEVNRLQ EKWEKERSWL QQELDKTLET LERERAELET KLREQQTEME
910 920 930 940 950
AIRAQREEER SQADSALYQM QLETEKERVS LLETLLRTQK ELADASQQLE
960 970 980 990 1000
RLRQDMKIQK LKEQETTGML QAQLQETQQE LKEAAQQHRD DLAAFQKDKL
1010 1020 1030 1040 1050
DLQKQVEDLM SQLVAHDDSQ RLVKEEIEEK VKVAQECSRI QKELEKENAS
1060 1070 1080 1090 1100
LALSLVEKEK RLLILQEADS VRQQELSSLR QDIQEAQEGQ RELGVQVELL
1110 1120 1130 1140 1150
RQEVKEKEAD FVAREAQLLE ELEASRVAEQ QLRASLWAQE AKATQLQLQL
1160 1170 1180 1190 1200
RSTESQLEAL VAEQQPENQA QAQLASLCSV LQQALGSACE SRPELRGGGD
1210 1220 1230 1240 1250
SAPTLWGPDP DQNGASRLFK RWSLPTALSP EAVALALQKL HQDVWKARQA
1260 1270 1280 1290 1300
RDDLRDQVQK LVQRLTDTEA QKSQVHSELQ DLQRQLSQSQ EEKSKWEGRQ
1310 1320 1330 1340 1350
NSLESELRDL HETAASLQSR LRQAELQKME AQNDRELLQA SKEKLSAQVE
1360 1370 1380 1390 1400
HLQACVAEAQ AQADAAAVLE EDLRTARSAL KLKNEELESE RERAQALQEQ
1410 1420 1430 1440 1450
GELKVAQGKA LQENLALLAQ TLSNREREVE TLQAEVQELE KQREMQKAAL
1460 1470 1480 1490 1500
ELLSLDLKKR SREVDLQQEQ IQELEQCRSV LEHLPMAVQE REQKLSVQRD
1510 1520 1530 1540 1550
QIRELENDRE AQRSVLEHQL LDLEQKAQVI ESQRGQIQDL KKQLGTLECL
1560 1570 1580 1590 1600
ALELEESHHK VESQQKMITE LEGQREMQRV ALTHLTLDLE ERSQELQAQS
1610 1620 1630 1640 1650
SQLHELENHS THLAKELQER DQEVTSQRQQ IDELQKQQEQ LAQALERKGQ
1660 1670 1680 1690 1700
ELVLQKERIQ VLEDQRTLQT KILEEDLEQI KHSLRERSQE LASQWQLVHE
1710 1720 1730 1740 1750
RADDGKSPSK GQRGSLEHLK LILRDKEKEV ECQQERIQEL QGHMGQLEQQ
1760 1770 1780 1790 1800
LQGLHRKVGE TSLLLTHREQ ETATLQQHLQ EAKEQGELRE QVLQGQLEEA
1810 1820 1830 1840 1850
QRDLAQRDHE LETLRQEKQQ TQDQEESMKL KTSALQAALE QAHATLKERQ
1860 1870 1880 1890 1900
GELEEHREQV RRLQEELEVE GRQVRALEEV LGDLRAESRE HEKAVLALQQ
1910 1920 1930 1940 1950
RCAEQAQEHE AEARTLQDSW LQAQATLTEQ EQELAALRAE NQYSRRQEEA
1960 1970 1980 1990 2000
AVSQAEALQE ALSKAQAALQ EKEQSLLEQA ELSHTLEAST AALQATLDTC
2010 2020 2030 2040 2050
QASARQLEEA LRIREGEIQA QALQHHEVTQ HLQQELCQKK EELRQLLEKA
2060 2070 2080 2090 2100
GARRSQENGI QEKQSLEQER QEETRRLLES LKELQLTVAQ REEEILMLRE
2110 2120 2130 2140 2150
ASSPRHRALP AEKPALQPLP AQQELERLQT ALRQTEAREI EWREKAQDLA
2160 2170 2180 2190 2200
LSLAQSKASI SSLQEITMFL QASVLERESE QQRLQEELVL SRQALEEQQS
2210 2220 2230 2240 2250
GGPHSTSRAD QGPKVGQGSQ SGEVETEPSP GVEEKERLTQ RLERLQQAVA
2260 2270 2280 2290 2300
ELEVDRSKLQ CHNAQLRTAL EQVERERRKL KRDSVRASRA GSLEARETMT
2310 2320 2330 2340 2350
SSPTQQDGRG SQRGSSDSVL VVELQREVAL LRAQLALERK QRQDYIARSV
2360 2370 2380 2390 2400
QTSRELAGLH HSLSHSLLTV AQAPEATVLE AETRKLDESL NQSLTSPGPC
2410
LLHPSLDTTQ NTHR
Length:2,414
Mass (Da):276,814
Last modified:July 27, 2011 - v4
Checksum:i7B76E9E781D731CC
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A3KGJ7A3KGJ7_MOUSE
Centrosome-associated protein CEP25...
Cep250
2,435Annotation score:
Q8BZX9Q8BZX9_MOUSE
Centrosome-associated protein CEP25...
Cep250 Cep2
338Annotation score:
E9Q5A8E9Q5A8_MOUSE
Centrosome-associated protein CEP25...
Cep250
2,434Annotation score:
B7ZCN3B7ZCN3_MOUSE
Centrosome-associated protein CEP25...
Cep250
706Annotation score:
F6QCB4F6QCB4_MOUSE
Centrosome-associated protein CEP25...
Cep250
138Annotation score:
F7BUJ0F7BUJ0_MOUSE
Centrosome-associated protein CEP25...
Cep250
224Annotation score:

Sequence cautioni

The sequence AAB41824 differs from that shown. Reason: Frameshift at positions 1843, 1914, 1924, 2341 and 2371.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91E → K in BAC32417 (PubMed:16141072).Curated1
Sequence conflicti369S → L in ABA29340 (PubMed:16339073).Curated1
Sequence conflicti424H → R in ABA29340 (PubMed:16339073).Curated1
Sequence conflicti437K → R in ABA29340 (PubMed:16339073).Curated1
Sequence conflicti454G → D in ABA29340 (PubMed:16339073).Curated1
Sequence conflicti508K → R in ABA29340 (PubMed:16339073).Curated1
Sequence conflicti615E → G in ABA29340 (PubMed:16339073).Curated1
Sequence conflicti645Q → R in ABA29340 (PubMed:16339073).Curated1
Sequence conflicti1837A → P in AAB41824 (PubMed:8950169).Curated1
Sequence conflicti1864Q → R in AAB41824 (PubMed:8950169).Curated1
Sequence conflicti1924 – 1925QA → RP in AAB41824 (PubMed:8950169).Curated2
Sequence conflicti1966Q → E in BAE23916 (PubMed:16141072).Curated1
Sequence conflicti1974Q → H in AAH57582 (PubMed:15489334).Curated1
Sequence conflicti2003S → R in ABA29340 (PubMed:16339073).Curated1
Sequence conflicti2003S → R in AAB41824 (PubMed:8950169).Curated1
Sequence conflicti2040K → E in ABA29340 (PubMed:16339073).Curated1
Sequence conflicti2040K → E in AAB41824 (PubMed:8950169).Curated1
Sequence conflicti2105R → S in ABA29340 (PubMed:16339073).Curated1
Sequence conflicti2105R → S in AAB41824 (PubMed:8950169).Curated1
Sequence conflicti2192R → S in AAB41824 (PubMed:8950169).Curated1
Sequence conflicti2218G → E in AAB41824 (PubMed:8950169).Curated1
Sequence conflicti2221S → P in ABA29340 (PubMed:16339073).Curated1
Sequence conflicti2221S → P in AAB41824 (PubMed:8950169).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ148475 mRNA Translation: ABA29340.1
AL833786 Genomic DNA No translation available.
AK045557 mRNA Translation: BAC32417.1
AK139187 mRNA Translation: BAE23916.1
U33198 mRNA Translation: AAB41824.1 Frameshift.
BC057582 mRNA Translation: AAH57582.1
CCDSiCCDS50771.1
RefSeqiNP_001123471.1, NM_001129999.1
NP_032409.3, NM_008383.3
NP_796191.2, NM_177217.3
UniGeneiMm.288729

Genome annotation databases

EnsembliENSMUST00000094421; ENSMUSP00000091988; ENSMUSG00000038241
GeneIDi16328
KEGGimmu:16328
UCSCiuc008nlu.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ148475 mRNA Translation: ABA29340.1
AL833786 Genomic DNA No translation available.
AK045557 mRNA Translation: BAC32417.1
AK139187 mRNA Translation: BAE23916.1
U33198 mRNA Translation: AAB41824.1 Frameshift.
BC057582 mRNA Translation: AAH57582.1
CCDSiCCDS50771.1
RefSeqiNP_001123471.1, NM_001129999.1
NP_032409.3, NM_008383.3
NP_796191.2, NM_177217.3
UniGeneiMm.288729

3D structure databases

SMRiQ60952
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200766, 6 interactors
IntActiQ60952, 8 interactors
STRINGi10090.ENSMUSP00000105248

PTM databases

iPTMnetiQ60952
PhosphoSitePlusiQ60952

Proteomic databases

EPDiQ60952
MaxQBiQ60952
PaxDbiQ60952
PeptideAtlasiQ60952
PRIDEiQ60952

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000094421; ENSMUSP00000091988; ENSMUSG00000038241
GeneIDi16328
KEGGimmu:16328
UCSCiuc008nlu.2 mouse

Organism-specific databases

CTDi11190
MGIiMGI:108084 Cep250

Phylogenomic databases

eggNOGiENOG410IFAN Eukaryota
ENOG410XPG3 LUCA
GeneTreeiENSGT00730000110800
HOGENOMiHOG000246955
HOVERGENiHBG081320
InParanoidiQ60952
KOiK16464
OMAiRIETSII

Enzyme and pathway databases

ReactomeiR-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-8854518 AURKA Activation by TPX2

Miscellaneous databases

ChiTaRSiCep250 mouse
PROiPR:Q60952
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038241 Expressed in 142 organ(s), highest expression level in olfactory epithelium
CleanExiMM_CEP250
ExpressionAtlasiQ60952 baseline and differential
GenevisibleiQ60952 MM

Family and domain databases

InterProiView protein in InterPro
IPR026048 CEP250
PANTHERiPTHR23159:SF1 PTHR23159:SF1, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCP250_MOUSE
AccessioniPrimary (citable) accession number: Q60952
Secondary accession number(s): E9QMB2
, Q2I8G3, Q3UTR4, Q6PFF6, Q8BLC6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 136 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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