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UniProtKB - Q60948 (MAD4_MOUSE)
Protein
Max dimerization protein 4
Gene
Mxd4
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
1 PublicationGO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Max dimerization protein 4Short name: Max dimerizer 4 Alternative name(s): Max-associated protein 4 Max-interacting transcriptional repressor MAD4 |
Gene namesi | Name:Mxd4 Synonyms:Mad4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104991, Mxd4 |
Subcellular locationi
Nucleus
Nucleus
- RNA polymerase II transcription regulator complex Source: NTNU_SB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127267 | 1 – 209 | Max dimerization protein 4Add BLAST | 209 |
Proteomic databases
MaxQBi | Q60948 |
PaxDbi | Q60948 |
PRIDEi | Q60948 |
ProteomicsDBi | 295760 |
PTM databases
PhosphoSitePlusi | Q60948 |
Expressioni
Developmental stagei
Expressed during neural and epidermal differentiation. Primarily expressed in growth-arrested differentiating cells. In the spinal cord at embryonic day 10.5, a strong expression seen in the differentiating cells of the intermediate zone at the ventral part of the neural tube and weakly in the ventricular zone. At 11.5 and 12.5 dpc, highly expressed in the intermediate zone and at reduced levels in the ventricular zone that mostly persists in the dorsal part of the neural tube. At 14.5 dpc, expressed throughout the spinal cord. In the developing epidermis, expressed in the dermis, hair follicles and in some differentiating cells in the upper layers of the epidermis.1 Publication
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX.
Interacts with SIN3A AND SIN3B.
Interacts with RNF17.
2 PublicationsGO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000039071 |
Miscellaneous databases
RNActi | Q60948, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 53 – 105 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 6 – 23 | Interaction with SIN3A and SIN3B1 PublicationAdd BLAST | 18 | |
Regioni | 136 – 209 | DisorderedSequence analysisAdd BLAST | 74 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 136 – 154 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 178 – 195 | Polar residuesSequence analysisAdd BLAST | 18 |
Phylogenomic databases
eggNOGi | KOG2483, Eukaryota |
InParanoidi | Q60948 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q60948-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELNSLLLLL EAAEYLERRD REAEHGYASM LPFDGDFARK KTKTAGLVRK
60 70 80 90 100
GPNNRSSHNE LEKHRRAKLR LYLEQLKQLG PLGPDSTRHT TLSLLKRAKM
110 120 130 140 150
HIKKLEEQDR RALSIKEQLQ REHRFLKRRL EQLSVQSVER VRTDSTGSAV
160 170 180 190 200
STDDSEQEVD IEGMEFGPGE LDSAGSSSDA DDHYSLQSSG CSDSSYGHPC
RRPGCPGLS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ91VN7 | Q91VN7_MOUSE | Max dimerization protein 4 | Mxd4 | 209 | Annotation score: | ||
Q3UZ22 | Q3UZ22_MOUSE | Max dimerization protein 4 | Mxd4 | 199 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U32395 mRNA Translation: AAB02795.1 |
CCDSi | CCDS19212.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U32395 mRNA Translation: AAB02795.1 |
CCDSi | CCDS19212.1 |
3D structure databases
AlphaFoldDBi | Q60948 |
SMRi | Q60948 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000039071 |
PTM databases
PhosphoSitePlusi | Q60948 |
Proteomic databases
MaxQBi | Q60948 |
PaxDbi | Q60948 |
PRIDEi | Q60948 |
ProteomicsDBi | 295760 |
Organism-specific databases
MGIi | MGI:104991, Mxd4 |
Phylogenomic databases
eggNOGi | KOG2483, Eukaryota |
InParanoidi | Q60948 |
Miscellaneous databases
ChiTaRSi | Mxd4, mouse |
PROi | PR:Q60948 |
RNActi | Q60948, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MAD4_MOUSE | |
Accessioni | Q60948Primary (citable) accession number: Q60948 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot