Mxd4

Functionⁱ

Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.1 Publication

GO - Molecular functionⁱ

DNA binding Source: UniProtKB-KW
protein dimerization activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

negative regulation of transcription, DNA-templated
Source: MGI
Inferred from genetic interactionⁱ
- 8521822
negative regulation of transcription by RNA polymerase II
Source: NTNU_SB
Inferred from direct assayⁱ
- 8521822

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Repressor
Biological process	Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Max dimerization protein 4 Short name: Max dimerizer 4 Alternative name(s): Max-associated protein 4 Max-interacting transcriptional repressor MAD4
Gene namesⁱ	Name:Mxd4 Synonyms:Mad4
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Unplaced

Organism-specific databases

MGIⁱ	MGI:104991 Mxd4

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127267	1 – 209	Max dimerization protein 4Add BLAST		209

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q60948
PaxDbⁱ	Q60948
PRIDEⁱ	Q60948

PTM databases

PhosphoSitePlusⁱ	Q60948

PhosphoSitePlusⁱ

Q60948

Expressionⁱ

Developmental stageⁱ

Expressed during neural and epidermal differentiation. Primarily expressed in growth-arrested differentiating cells. In the spinal cord at embryonic day 10.5, a strong expression seen in the differentiating cells of the intermediate zone at the ventral part of the neural tube and weakly in the ventricular zone. At 11.5 and 12.5 dpc, highly expressed in the intermediate zone and at reduced levels in the ventricular zone that mostly persists in the dorsal part of the neural tube. At 14.5 dpc, expressed throughout the spinal cord. In the developing epidermis, expressed in the dermis, hair follicles and in some differentiating cells in the upper layers of the epidermis.1 Publication

Gene expression databases

CleanExⁱ	MM_MXD4

CleanExⁱ

MM_MXD4

Interactionⁱ

Subunit structureⁱ

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SIN3A AND SIN3B. Interacts with RNF17.2 Publications

GO - Molecular functionⁱ

protein dimerization activity Source: InterPro

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000039071

STRINGⁱ

10090.ENSMUSP00000039071

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q60948
SMRⁱ	Q60948
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	53 – 105	bHLHPROSITE-ProRule annotationAdd BLAST		53

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	6 – 23	Interaction with SIN3A and SIN3B1 PublicationAdd BLAST		18

Phylogenomic databases

eggNOGⁱ	KOG2483 Eukaryota ENOG4111V1C LUCA
HOVERGENⁱ	HBG006314
InParanoidⁱ	Q60948

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show all Align All

Q60948-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MELNSLLLLL EAAEYLERRD REAEHGYASM LPFDGDFARK KTKTAGLVRK 
        60         70         80         90        100
GPNNRSSHNE LEKHRRAKLR LYLEQLKQLG PLGPDSTRHT TLSLLKRAKM 
       110        120        130        140        150
HIKKLEEQDR RALSIKEQLQ REHRFLKRRL EQLSVQSVER VRTDSTGSAV 
       160        170        180        190        200
STDDSEQEVD IEGMEFGPGE LDSAGSSSDA DDHYSLQSSG CSDSSYGHPC 

RRPGCPGLS

Length:209

Mass (Da):23,590

Last modified:November 1, 1996 - v1

Checksum:ⁱC8FB5A56AFE3B27C

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

Q91VN7	Q91VN7_MOUSE	Max dimerization protein 4 Max dimerization protein 4	Mxd4	209	Annotation score:
Q3UZ22	Q3UZ22_MOUSE	Max dimerization protein 4 Max dimerization protein 4	Mxd4	199	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U32395 mRNA Translation: AAB02795.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS19212.1
UniGeneⁱ	Mm.259260 Mm.391777

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q60948	Uncharacterized protein		CAVPO		209	UniRef90_Q60948
Uncharacterized protein		PIG		209
Max dimerization protein 4		CASCN		209
Uncharacterized protein		PROCO		209
Uncharacterized protein		FELCA		209
+36

Protein	Similar proteins		Species	Score	Length	Source
Q60948	Max dimerization protein 4		HUMAN		209	UniRef50_Q60948
Max dimerization protein 4		HETGA		209
Uncharacterized protein		CAVPO		209
MAX dimerization protein 4		GORGO		209
MAD4		BOVIN		209
+89

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U32395 mRNA Translation: AAB02795.1
CCDSⁱ	CCDS19212.1
UniGeneⁱ	Mm.259260 Mm.391777

3D structure databases

ProteinModelPortalⁱ	Q60948
SMRⁱ	Q60948
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000039071

STRINGⁱ

10090.ENSMUSP00000039071

PTM databases

PhosphoSitePlusⁱ	Q60948

PhosphoSitePlusⁱ

Q60948

Proteomic databases

MaxQBⁱ	Q60948
PaxDbⁱ	Q60948
PRIDEⁱ	Q60948

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Organism-specific databases

MGIⁱ	MGI:104991 Mxd4

Phylogenomic databases

eggNOGⁱ	KOG2483 Eukaryota ENOG4111V1C LUCA
HOVERGENⁱ	HBG006314
InParanoidⁱ	Q60948

Miscellaneous databases

ChiTaRSⁱ	Mxd4 mouse
Protein Ontology More...PROⁱ	PR:Q60948
SOURCEⁱ	Search...

Gene expression databases

CleanExⁱ	MM_MXD4

CleanExⁱ

MM_MXD4

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf
Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MAD4_MOUSE
Accessionⁱ	Q60948Primary (citable) accession number: Q60948
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
	Last sequence update:	November 1, 1996
	Last modified:	November 7, 2018
	This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q60948 (MAD4_MOUSE)

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Max dimerization protein 4

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Phylogenomic databases

Family and domain databases

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ