UniProtKB - Q60928 (GGT1_MOUSE)
Glutathione hydrolase 1 proenzyme
Ggt1
Functioni
Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates and other gamma-glutamyl compounds, such as leukotriene C4 (LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4 (By similarity).
Indirectly regulates multiple aspects of skeletal biology (PubMed:12810527).
By similarity1 PublicationCatalytic activityi
- an α-amino acid + an N-terminal (5-L-glutamyl)-[peptide] = 5-L-glutamyl amino acid + N-terminal L-α-aminoacyl-[peptide]By similarityEC:2.3.2.2By similarity
- EC:3.4.19.13By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:3.4.19.13By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:3.4.19.14By similarityThis reaction proceeds in the forwardBy similarity direction.
Activity regulationi
: glutathione metabolism Pathwayi
This protein is involved in the pathway glutathione metabolism, which is part of Sulfur metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway glutathione metabolism and in Sulfur metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 106 | GlutamateBy similarity | 1 | |
Active sitei | 380 | NucleophileBy similarity | 1 | |
Binding sitei | 419 | GlutamateBy similarity | 1 | |
Binding sitei | 422 | GlutamateBy similarity | 1 | |
Binding sitei | 473 | Glutamate; via amide nitrogenBy similarity | 1 |
GO - Molecular functioni
- aminoacyltransferase activity Source: MGI
- glutathione hydrolase activity Source: UniProtKB
- hypoglycin A gamma-glutamyl transpeptidase activity Source: UniProtKB-EC
- leukotriene-C(4) hydrolase Source: MGI
- leukotriene C4 gamma-glutamyl transferase activity Source: UniProtKB-EC
- peptidyltransferase activity Source: MGI
GO - Biological processi
- aging Source: Ensembl
- cellular response to oxidative stress Source: Ensembl
- cysteine biosynthetic process Source: UniProtKB
- glutamate metabolic process Source: UniProtKB
- glutathione biosynthetic process Source: UniProtKB
- glutathione catabolic process Source: UniProtKB
- glutathione metabolic process Source: MGI
- hepatoblast differentiation Source: Ensembl
- hepatocyte differentiation Source: Ensembl
- liver regeneration Source: Ensembl
- peptide modification Source: MGI
- regulation of immune system process Source: UniProtKB
- regulation of inflammatory response Source: UniProtKB
- response to alcohol Source: Ensembl
- response to estradiol Source: GO_Central
- response to lipopolysaccharide Source: GO_Central
- response to tumor necrosis factor Source: GO_Central
- spermatogenesis Source: MGI
- zymogen activation Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Hydrolase, Protease, Transferase |
Biological process | Glutathione biosynthesis |
Enzyme and pathway databases
Reactomei | R-MMU-174403, Glutathione synthesis and recycling R-MMU-2142691, Synthesis of Leukotrienes (LT) and Eoxins (EX) R-MMU-5423646, Aflatoxin activation and detoxification |
SABIO-RKi | Q60928 |
UniPathwayi | UPA00204 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Ggt1 Synonyms:Ggt, Ggtp |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95706, Ggt1 |
VEuPathDBi | HostDB:ENSMUSG00000006345 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type II membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Extracellular region or secreted
- extracellular space Source: MGI
Plasma Membrane
- integral component of plasma membrane Source: MGI
- plasma membrane Source: UniProtKB
Other locations
- perinuclear region of cytoplasm Source: MGI
- vesicle Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 4 | CytoplasmicBy similarity | 4 | |
Transmembranei | 5 – 26 | Helical; Signal-anchor for type II membrane proteinBy similarityAdd BLAST | 22 | |
Topological domaini | 27 – 568 | ExtracellularBy similarityAdd BLAST | 542 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Involvement in diseasei
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000011060 | 1 – 379 | Glutathione hydrolase 1 heavy chainAdd BLAST | 379 | |
ChainiPRO_0000011061 | 380 – 568 | Glutathione hydrolase 1 light chainAdd BLAST | 189 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 49 ↔ 73 | By similarity | ||
Glycosylationi | 94 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 114 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 119 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 191 ↔ 195 | By similarity | ||
Glycosylationi | 229 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 343 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 510 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
jPOSTi | Q60928 |
MaxQBi | Q60928 |
PaxDbi | Q60928 |
PRIDEi | Q60928 |
ProteomicsDBi | 266802 |
TopDownProteomicsi | Q60928 |
PTM databases
GlyConnecti | 2333, 1 N-Linked glycan (1 site) |
GlyGeni | Q60928, 6 sites, 1 N-linked glycan (1 site) |
iPTMneti | Q60928 |
PhosphoSitePlusi | Q60928 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000006345, Expressed in proximal tubule and 163 other tissues |
Genevisiblei | Q60928, MM |
Interactioni
Subunit structurei
Heterodimer composed of the light and heavy chains. The active site is located in the light chain.
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000006508 |
Miscellaneous databases
RNActi | Q60928, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 398 – 400 | Glutamate bindingBy similarity | 3 | |
Regioni | 450 – 451 | Glutamate bindingBy similarity | 2 |
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2410, Eukaryota |
GeneTreei | ENSGT00940000154601 |
HOGENOMi | CLU_014813_4_1_1 |
InParanoidi | Q60928 |
OMAi | KATKNMF |
OrthoDBi | 1419292at2759 |
PhylomeDBi | Q60928 |
TreeFami | TF313608 |
Family and domain databases
Gene3Di | 1.10.246.130, 1 hit 3.60.20.40, 1 hit |
InterProi | View protein in InterPro IPR043138, GGT_lsub_C IPR000101, GGT_peptidase IPR043137, GGT_ssub IPR029055, Ntn_hydrolases_N |
PANTHERi | PTHR11686, PTHR11686, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR00066, g_glut_trans, 1 hit |
PROSITEi | View protein in PROSITE PS00462, G_GLU_TRANSPEPTIDASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 13 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MKNRFLVLGL VAVVLVFVII GLCIWLPYTS GKPDHVYSRA AVATDAKRCS
60 70 80 90 100
EIGRDILQEG GSVVDAAIAS LLCMGLMNAH SMGIGGGLFF TIYNSTTGKV
110 120 130 140 150
EVINAREVAP RLANTTMFNN SKDSEEGGLS VAVPGEIRGY ELAHQRHGRL
160 170 180 190 200
PWARLFQPSI QLARHGFPVG KGLAIALDKK RDVIEKTPAL CEVFCRQGKV
210 220 230 240 250
LQEGETVTMP KLADTLQILA QEGAKAFYNG SLTAQIVKDI QEAGGIMTVE
260 270 280 290 300
DLNNYRAELI EHPMSIGLGD ATLYVPSAPL SGPVLILILN ILKGYNFSPK
310 320 330 340 350
SVATPEQKAL TYHRIVEAFR FAYAKRTMLG DPKFVDVSQV IRNMSSEFYA
360 370 380 390 400
TQLRARITDE TTHPAAYYEP EFYLQDDGGT AHLSAVSEDG SAVAATSTIN
410 420 430 440 450
LYFGSKVLSR VSGILFNDEM DDFSSPNFIN QFRVAPSPAN FIKPGKQPLS
460 470 480 490 500
SMCPSIILDK DGQVRMVVGA SGGTQITTSV ALAIINSLWF GYDVKRAVEE
510 520 530 540 550
PRLHNQLLPN TTTVEKDIDQ VVTAGLKIRH HHTEVTPTFI AVVQAVVRAS
560
GGWAAASDSR KGGEPAGY
Computationally mapped potential isoform sequencesi
There are 13 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6V328 | F6V328_MOUSE | Glutathione hydrolase 1 proenzyme | Ggt1 | 130 | Annotation score: | ||
D3Z266 | D3Z266_MOUSE | Glutathione hydrolase 1 proenzyme | Ggt1 | 243 | Annotation score: | ||
D3YVY4 | D3YVY4_MOUSE | Glutathione hydrolase 1 proenzyme | Ggt1 | 237 | Annotation score: | ||
D3YWW2 | D3YWW2_MOUSE | Glutathione hydrolase 1 proenzyme | Ggt1 | 189 | Annotation score: | ||
D3YWW4 | D3YWW4_MOUSE | Glutathione hydrolase 1 proenzyme | Ggt1 | 190 | Annotation score: | ||
D3Z4Q6 | D3Z4Q6_MOUSE | Glutathione hydrolase 1 proenzyme | Ggt1 | 235 | Annotation score: | ||
D3YVX1 | D3YVX1_MOUSE | Glutathione hydrolase 1 proenzyme | Ggt1 | 88 | Annotation score: | ||
D3YY79 | D3YY79_MOUSE | Glutathione hydrolase 1 proenzyme | Ggt1 | 209 | Annotation score: | ||
D3Z2C6 | D3Z2C6_MOUSE | Glutathione hydrolase 1 proenzyme | Ggt1 | 60 | Annotation score: | ||
D3YYH6 | D3YYH6_MOUSE | Glutathione hydrolase 1 proenzyme | Ggt1 | 110 | Annotation score: | ||
There are more potential isoformsShow all |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U30509 mRNA Translation: AAA97395.1 BC012969 mRNA Translation: AAH12969.1 |
CCDSi | CCDS23927.1 |
PIRi | JC4570 |
RefSeqi | NP_001292921.1, NM_001305992.1 NP_032142.1, NM_008116.3 XP_006513293.1, XM_006513230.3 XP_006513294.1, XM_006513231.3 |
Genome annotation databases
Ensembli | ENSMUST00000006508; ENSMUSP00000006508; ENSMUSG00000006345 ENSMUST00000134503; ENSMUSP00000121312; ENSMUSG00000006345 |
GeneIDi | 14598 |
KEGGi | mmu:14598 |
UCSCi | uc007fqo.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U30509 mRNA Translation: AAA97395.1 BC012969 mRNA Translation: AAH12969.1 |
CCDSi | CCDS23927.1 |
PIRi | JC4570 |
RefSeqi | NP_001292921.1, NM_001305992.1 NP_032142.1, NM_008116.3 XP_006513293.1, XM_006513230.3 XP_006513294.1, XM_006513231.3 |
3D structure databases
SMRi | Q60928 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000006508 |
PTM databases
GlyConnecti | 2333, 1 N-Linked glycan (1 site) |
GlyGeni | Q60928, 6 sites, 1 N-linked glycan (1 site) |
iPTMneti | Q60928 |
PhosphoSitePlusi | Q60928 |
Proteomic databases
jPOSTi | Q60928 |
MaxQBi | Q60928 |
PaxDbi | Q60928 |
PRIDEi | Q60928 |
ProteomicsDBi | 266802 |
TopDownProteomicsi | Q60928 |
Protocols and materials databases
DNASUi | 14598 |
Genome annotation databases
Ensembli | ENSMUST00000006508; ENSMUSP00000006508; ENSMUSG00000006345 ENSMUST00000134503; ENSMUSP00000121312; ENSMUSG00000006345 |
GeneIDi | 14598 |
KEGGi | mmu:14598 |
UCSCi | uc007fqo.2, mouse |
Organism-specific databases
CTDi | 2678 |
MGIi | MGI:95706, Ggt1 |
VEuPathDBi | HostDB:ENSMUSG00000006345 |
Phylogenomic databases
eggNOGi | KOG2410, Eukaryota |
GeneTreei | ENSGT00940000154601 |
HOGENOMi | CLU_014813_4_1_1 |
InParanoidi | Q60928 |
OMAi | KATKNMF |
OrthoDBi | 1419292at2759 |
PhylomeDBi | Q60928 |
TreeFami | TF313608 |
Enzyme and pathway databases
UniPathwayi | UPA00204 |
Reactomei | R-MMU-174403, Glutathione synthesis and recycling R-MMU-2142691, Synthesis of Leukotrienes (LT) and Eoxins (EX) R-MMU-5423646, Aflatoxin activation and detoxification |
SABIO-RKi | Q60928 |
Miscellaneous databases
BioGRID-ORCSi | 14598, 1 hit in 65 CRISPR screens |
ChiTaRSi | Ggt1, mouse |
PROi | PR:Q60928 |
RNActi | Q60928, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000006345, Expressed in proximal tubule and 163 other tissues |
Genevisiblei | Q60928, MM |
Family and domain databases
Gene3Di | 1.10.246.130, 1 hit 3.60.20.40, 1 hit |
InterProi | View protein in InterPro IPR043138, GGT_lsub_C IPR000101, GGT_peptidase IPR043137, GGT_ssub IPR029055, Ntn_hydrolases_N |
PANTHERi | PTHR11686, PTHR11686, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR00066, g_glut_trans, 1 hit |
PROSITEi | View protein in PROSITE PS00462, G_GLU_TRANSPEPTIDASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GGT1_MOUSE | |
Accessioni | Q60928Primary (citable) accession number: Q60928 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families