UniProtKB - Q60876 (4EBP1_MOUSE)
Eukaryotic translation initiation factor 4E-binding protein 1
Eif4ebp1
Functioni
Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation (By similarity).
Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways (PubMed:7629182).
By similarity1 PublicationGO - Molecular functioni
- eukaryotic initiation factor 4E binding Source: MGI
- protein phosphatase 2A binding Source: MGI
- translation initiation factor binding Source: MGI
- translation repressor activity Source: UniProtKB
GO - Biological processi
- cellular response to dexamethasone stimulus Source: MGI
- cellular response to hypoxia Source: MGI
- G1/S transition of mitotic cell cycle Source: MGI
- insulin receptor signaling pathway Source: MGI
- IRES-dependent translational initiation of linear mRNA Source: ParkinsonsUK-UCL
- lung development Source: Ensembl
- negative regulation of protein-containing complex assembly Source: MGI
- negative regulation of translational initiation Source: MGI
- positive regulation of mitotic cell cycle Source: MGI
- regulation of translational initiation Source: MGI
- response to amino acid starvation Source: MGI
- response to ethanol Source: Ensembl
- response to ischemia Source: Ensembl
- TOR signaling Source: UniProtKB
Keywordsi
Molecular function | Protein synthesis inhibitor |
Biological process | Translation regulation |
Enzyme and pathway databases
Reactomei | R-MMU-166208, mTORC1-mediated signalling R-MMU-72662, Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic translation initiation factor 4E-binding protein 1Short name: 4E-BP1 Short name: eIF4E-binding protein 1 Alternative name(s): Phosphorylated heat- and acid-stable protein regulated by insulin 1 Short name: PHAS-I |
Gene namesi | Name:Eif4ebp1 |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:103267, Eif4ebp1 |
VEuPathDBi | HostDB:ENSMUSG00000031490 |
Subcellular locationi
Cytosol
- cytosol Source: AgBase
- postsynaptic cytosol Source: MGI
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- glutamatergic synapse Source: MGI
- protein-containing complex Source: MGI
Pathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 113 | F → A: Impaired interaction with RPTOR. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000190514 | 2 – 117 | Eukaryotic translation initiation factor 4E-binding protein 1Add BLAST | 116 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 36 | PhosphothreonineCombined sources1 Publication | 1 | |
Modified residuei | 40 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 43 | PhosphoserineCombined sources | 1 | |
Modified residuei | 45 | PhosphothreonineCombined sources1 Publication | 1 | |
Modified residuei | 49 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 53 | PhosphotyrosineBy similarity | 1 | |
Cross-linki | 56 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 64 | PhosphoserineCombined sources | 1 | |
Modified residuei | 69 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 82 | PhosphoserineBy similarity | 1 | |
Modified residuei | 95 | PhosphoserineCombined sources | 1 | |
Modified residuei | 99 | PhosphoserineCombined sources | 1 | |
Modified residuei | 100 | PhosphoserineCombined sources | 1 | |
Modified residuei | 111 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q60876 |
jPOSTi | Q60876 |
MaxQBi | Q60876 |
PaxDbi | Q60876 |
PeptideAtlasi | Q60876 |
PRIDEi | Q60876 |
ProteomicsDBi | 285687 |
PTM databases
iPTMneti | Q60876 |
PhosphoSitePlusi | Q60876 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000031490, Expressed in white adipose tissue and 268 other tissues |
Genevisiblei | Q60876, MM |
Interactioni
Subunit structurei
Hypophosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation (PubMed:7629182).
Interacts (via TOS motif) with RPTOR; promoting phosphorylation by mTORC1 (PubMed:24139800).
2 PublicationsBinary interactionsi
Q60876
With | #Exp. | IntAct |
---|---|---|
Eif4e [P63073] | 3 | EBI-398674,EBI-2000006 |
GO - Molecular functioni
- eukaryotic initiation factor 4E binding Source: MGI
- protein phosphatase 2A binding Source: MGI
- translation initiation factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199421, 11 interactors |
DIPi | DIP-32577N |
IntActi | Q60876, 4 interactors |
MINTi | Q60876 |
STRINGi | 10090.ENSMUSP00000033880 |
Miscellaneous databases
RNActi | Q60876, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 47 | DisorderedSequence analysisAdd BLAST | 47 | |
Regioni | 64 – 117 | DisorderedSequence analysisAdd BLAST | 54 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 53 – 59 | YXXXXLphi motifBy similarity | 7 | |
Motifi | 113 – 117 | TOS motif1 Publication | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 15 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 33 – 47 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 84 – 101 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 103 – 117 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502S4SY, Eukaryota |
GeneTreei | ENSGT00940000159932 |
HOGENOMi | CLU_111706_0_0_1 |
InParanoidi | Q60876 |
OMAi | QMPDVYS |
OrthoDBi | 1597535at2759 |
PhylomeDBi | Q60876 |
TreeFami | TF101530 |
Family and domain databases
InterProi | View protein in InterPro IPR008606, EIF4EBP IPR037582, EIF4EBP1 |
PANTHERi | PTHR12669:SF14, PTHR12669:SF14, 1 hit |
Pfami | View protein in Pfam PF05456, eIF_4EBP, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSAGSSCSQT PSRAIPTRRV ALGDGVQLPP GDYSTTPGGT LFSTTPGGTR
60 70 80 90 100
IIYDRKFLME CRNSPVAKTP PKDLPAIPGV TSPTSDEPPM QASQSQLPSS
110
PEDKRAGGEE SQFEMDI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 93 | S → N in BAB28612 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28656 mRNA Translation: AAA88818.1 AK013033 mRNA Translation: BAB28612.1 AK169979 mRNA Translation: BAE41494.1 AK170031 mRNA Translation: BAE41521.1 BC002045 mRNA Translation: AAH02045.1 |
CCDSi | CCDS22214.1 |
PIRi | A57396 |
RefSeqi | NP_031944.3, NM_007918.3 |
Genome annotation databases
Ensembli | ENSMUST00000033880; ENSMUSP00000033880; ENSMUSG00000031490 |
GeneIDi | 13685 |
KEGGi | mmu:13685 |
UCSCi | uc009lih.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28656 mRNA Translation: AAA88818.1 AK013033 mRNA Translation: BAB28612.1 AK169979 mRNA Translation: BAE41494.1 AK170031 mRNA Translation: BAE41521.1 BC002045 mRNA Translation: AAH02045.1 |
CCDSi | CCDS22214.1 |
PIRi | A57396 |
RefSeqi | NP_031944.3, NM_007918.3 |
3D structure databases
AlphaFoldDBi | Q60876 |
SMRi | Q60876 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199421, 11 interactors |
DIPi | DIP-32577N |
IntActi | Q60876, 4 interactors |
MINTi | Q60876 |
STRINGi | 10090.ENSMUSP00000033880 |
PTM databases
iPTMneti | Q60876 |
PhosphoSitePlusi | Q60876 |
Proteomic databases
EPDi | Q60876 |
jPOSTi | Q60876 |
MaxQBi | Q60876 |
PaxDbi | Q60876 |
PeptideAtlasi | Q60876 |
PRIDEi | Q60876 |
ProteomicsDBi | 285687 |
Protocols and materials databases
Antibodypediai | 3558, 2064 antibodies from 48 providers |
DNASUi | 13685 |
Genome annotation databases
Ensembli | ENSMUST00000033880; ENSMUSP00000033880; ENSMUSG00000031490 |
GeneIDi | 13685 |
KEGGi | mmu:13685 |
UCSCi | uc009lih.1, mouse |
Organism-specific databases
CTDi | 1978 |
MGIi | MGI:103267, Eif4ebp1 |
VEuPathDBi | HostDB:ENSMUSG00000031490 |
Phylogenomic databases
eggNOGi | ENOG502S4SY, Eukaryota |
GeneTreei | ENSGT00940000159932 |
HOGENOMi | CLU_111706_0_0_1 |
InParanoidi | Q60876 |
OMAi | QMPDVYS |
OrthoDBi | 1597535at2759 |
PhylomeDBi | Q60876 |
TreeFami | TF101530 |
Enzyme and pathway databases
Reactomei | R-MMU-166208, mTORC1-mediated signalling R-MMU-72662, Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S |
Miscellaneous databases
BioGRID-ORCSi | 13685, 1 hit in 72 CRISPR screens |
ChiTaRSi | Eif4ebp1, mouse |
PROi | PR:Q60876 |
RNActi | Q60876, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031490, Expressed in white adipose tissue and 268 other tissues |
Genevisiblei | Q60876, MM |
Family and domain databases
InterProi | View protein in InterPro IPR008606, EIF4EBP IPR037582, EIF4EBP1 |
PANTHERi | PTHR12669:SF14, PTHR12669:SF14, 1 hit |
Pfami | View protein in Pfam PF05456, eIF_4EBP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | 4EBP1_MOUSE | |
Accessioni | Q60876Primary (citable) accession number: Q60876 Secondary accession number(s): Q3TDS8, Q9CZ40 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2003 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 159 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families