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Protein

Stress-induced-phosphoprotein 1

Gene

Stip1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a co-chaperone for HSP90AA1. Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90.By similarity

GO - Molecular functioni

Enzyme and pathway databases

ReactomeiR-MMU-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)

Names & Taxonomyi

Protein namesi
Recommended name:
Stress-induced-phosphoprotein 1
Short name:
STI1
Short name:
mSTI1
Alternative name(s):
Hsc70/Hsp90-organizing protein
Short name:
Hop
Gene namesi
Name:Stip1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:109130 Stip1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001063731 – 543Stress-induced-phosphoprotein 1Add BLAST543

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei8N6-acetyllysineBy similarity1
Modified residuei16PhosphoserineBy similarity1
Cross-linki123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei198PhosphothreonineBy similarity1
Cross-linki210Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki210Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei301N6-acetyllysineBy similarity1
Modified residuei312N6-acetyllysineBy similarity1
Modified residuei325N6-acetyllysineCombined sources1
Modified residuei332PhosphothreonineBy similarity1
Modified residuei344N6-acetyllysineBy similarity1
Modified residuei354PhosphotyrosineBy similarity1
Modified residuei446N6-acetyllysineBy similarity1
Modified residuei481PhosphoserineBy similarity1

Post-translational modificationi

In vitro kinase assay failed to detect phosphorylation by MAPKAPK2.

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ60864
MaxQBiQ60864
PaxDbiQ60864
PeptideAtlasiQ60864
PRIDEiQ60864

2D gel databases

REPRODUCTION-2DPAGEiIPI00121514
Q60864
UCD-2DPAGEiQ60864

PTM databases

iPTMnetiQ60864
PhosphoSitePlusiQ60864
SwissPalmiQ60864

Expressioni

Gene expression databases

BgeeiENSMUSG00000024966 Expressed in 304 organ(s), highest expression level in morula
CleanExiMM_STIP1
GenevisibleiQ60864 MM

Interactioni

Subunit structurei

Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2 (By similarity). Forms a complex with HSPA8/HSC70, HSPCA/HSP-86 and HSPCB/HSP-84 (PubMed:8999875). Interacts with PACRG. Interacts with METTL21B. Interacts with HSP90/HSP90AA1; the interaction dissociates the PPP5C:HSP90AA1 interaction. Interacts with FLCN, FNIP1 and FNIP2. Interacts with HSPA8/HSC70 (By similarity). Interacts with HSP90AB1; upon SMYD2-dependent HSP90AB1 methylation (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203539, 7 interactors
IntActiQ60864, 7 interactors
MINTiQ60864
STRINGi10090.ENSMUSP00000025918

Structurei

3D structure databases

ProteinModelPortaliQ60864
SMRiQ60864
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati4 – 37TPR 1Add BLAST34
Repeati39 – 71TPR 2Add BLAST33
Repeati73 – 105TPR 3Add BLAST33
Domaini130 – 169STI1 1Add BLAST40
Repeati225 – 258TPR 4Add BLAST34
Repeati260 – 292TPR 5Add BLAST33
Repeati300 – 333TPR 6Add BLAST34
Repeati360 – 393TPR 7Add BLAST34
Repeati395 – 427TPR 8Add BLAST33
Repeati428 – 461TPR 9Add BLAST34
Domaini492 – 531STI1 2Add BLAST40

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi222 – 239Bipartite nuclear localization signalSequence analysisAdd BLAST18

Domaini

The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90 (By similarity).By similarity

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG0548 Eukaryota
ENOG410XTCJ LUCA
GeneTreeiENSGT00900000140989
HOGENOMiHOG000186562
HOVERGENiHBG057820
InParanoidiQ60864
KOiK09553
OMAiKDPAMRM
OrthoDBiEOG091G0E5W
PhylomeDBiQ60864
TreeFamiTF300478

Family and domain databases

Gene3Di1.25.40.10, 3 hits
InterProiView protein in InterPro
IPR006636 STI1_HS-bd
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR001440 TPR_1
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF00515 TPR_1, 1 hit
PF13181 TPR_8, 1 hit
SMARTiView protein in SMART
SM00727 STI1, 2 hits
SM00028 TPR, 9 hits
SUPFAMiSSF48452 SSF48452, 3 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 9 hits
PS50293 TPR_REGION, 2 hits

Sequencei

Sequence statusi: Complete.

Q60864-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEQVNELKEK GNKALSAGNI DDALQCYSEA IKLDPQNHVL YSNRSAAYAK
60 70 80 90 100
KGDYQKAYED GCKTVDLKPD WGKGYSRKAA ALEFLNRFEE AKRTYEEGLK
110 120 130 140 150
HEANNLQLKE GLQNMEARLA ERKFMNPFNL PNLYQKLEND PRTRSLLSDP
160 170 180 190 200
TYRELIEQLQ NKPSDLGTKL QDPRVMTTLS VLLGVDLGSM DEEEEAATPP
210 220 230 240 250
PPPPPKKEPK PEPMEEDLPE NKKQALKEKE LGNDAYKKKD FDKALKHYDR
260 270 280 290 300
AKELDPTNMT YITNQAAVHF EKGDYNKCRE LCEKAIEVGR ENREDYRQIA
310 320 330 340 350
KAYARIGNSY FKEEKYKDAI HFYNKSLAEH RTPDVLKKCQ QAEKILKEQE
360 370 380 390 400
RLAYINPDLA LEEKNKGNEC FQKGDYPQAM KHYTEAIKRN PRDAKLYSNR
410 420 430 440 450
AACYTKLLEF QLALKDCEEC IQLEPTFIKG YTRKAAALEA MKDYTKAMDV
460 470 480 490 500
YQKALDLDSS CKEAADGYQR CMMAQYNRHD SPEDVKRRAM ADPEVQQIMS
510 520 530 540
DPAMRLILEQ MQKDPQALSE HLKNPVIAQK IQKLMDVGLI AIR
Length:543
Mass (Da):62,582
Last modified:November 1, 1996 - v1
Checksum:iB737FBA92B198D6C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15L → P in BAC36100 (PubMed:16141072).Curated1
Sequence conflicti106L → V in AAH03794 (PubMed:15489334).Curated1
Sequence conflicti274 – 279DYNKCR → RLYKCT in BAC36100 (PubMed:16141072).Curated6
Sequence conflicti288V → S in BAC36100 (PubMed:16141072).Curated1
Sequence conflicti305 – 310RIGNSY → PNWQFL in BAC36100 (PubMed:16141072).Curated6
Sequence conflicti316 – 317YK → VQ in BAC36100 (PubMed:16141072).Curated2
Sequence conflicti333 – 347PDVLK…EKILK → QMCSRSASSQRNSE in BAC36100 (PubMed:16141072).CuratedAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27830 mRNA Translation: AAC53267.1
AK075988 mRNA Translation: BAC36100.1
AK088494 mRNA Translation: BAC40389.1
AK149493 mRNA Translation: BAE28916.1
AK161645 mRNA Translation: BAE36509.1
AK167273 mRNA Translation: BAE39385.1
BC003794 mRNA Translation: AAH03794.1
CCDSiCCDS37901.1
RefSeqiNP_058017.1, NM_016737.2
UniGeneiMm.258633

Genome annotation databases

EnsembliENSMUST00000025918; ENSMUSP00000025918; ENSMUSG00000024966
GeneIDi20867
KEGGimmu:20867
UCSCiuc008gke.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27830 mRNA Translation: AAC53267.1
AK075988 mRNA Translation: BAC36100.1
AK088494 mRNA Translation: BAC40389.1
AK149493 mRNA Translation: BAE28916.1
AK161645 mRNA Translation: BAE36509.1
AK167273 mRNA Translation: BAE39385.1
BC003794 mRNA Translation: AAH03794.1
CCDSiCCDS37901.1
RefSeqiNP_058017.1, NM_016737.2
UniGeneiMm.258633

3D structure databases

ProteinModelPortaliQ60864
SMRiQ60864
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203539, 7 interactors
IntActiQ60864, 7 interactors
MINTiQ60864
STRINGi10090.ENSMUSP00000025918

PTM databases

iPTMnetiQ60864
PhosphoSitePlusiQ60864
SwissPalmiQ60864

2D gel databases

REPRODUCTION-2DPAGEiIPI00121514
Q60864
UCD-2DPAGEiQ60864

Proteomic databases

EPDiQ60864
MaxQBiQ60864
PaxDbiQ60864
PeptideAtlasiQ60864
PRIDEiQ60864

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025918; ENSMUSP00000025918; ENSMUSG00000024966
GeneIDi20867
KEGGimmu:20867
UCSCiuc008gke.2 mouse

Organism-specific databases

CTDi10963
MGIiMGI:109130 Stip1

Phylogenomic databases

eggNOGiKOG0548 Eukaryota
ENOG410XTCJ LUCA
GeneTreeiENSGT00900000140989
HOGENOMiHOG000186562
HOVERGENiHBG057820
InParanoidiQ60864
KOiK09553
OMAiKDPAMRM
OrthoDBiEOG091G0E5W
PhylomeDBiQ60864
TreeFamiTF300478

Enzyme and pathway databases

ReactomeiR-MMU-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)

Miscellaneous databases

ChiTaRSiStip1 mouse
PROiPR:Q60864
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024966 Expressed in 304 organ(s), highest expression level in morula
CleanExiMM_STIP1
GenevisibleiQ60864 MM

Family and domain databases

Gene3Di1.25.40.10, 3 hits
InterProiView protein in InterPro
IPR006636 STI1_HS-bd
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR001440 TPR_1
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF00515 TPR_1, 1 hit
PF13181 TPR_8, 1 hit
SMARTiView protein in SMART
SM00727 STI1, 2 hits
SM00028 TPR, 9 hits
SUPFAMiSSF48452 SSF48452, 3 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 9 hits
PS50293 TPR_REGION, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiSTIP1_MOUSE
AccessioniPrimary (citable) accession number: Q60864
Secondary accession number(s): Q3TT16, Q8BPH3, Q99L66
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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