UniProtKB - Q60848 (HELLS_MOUSE)
Protein
Lymphocyte-specific helicase
Gene
Hells
Organism
Mus musculus (Mouse)
Status
Functioni
Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis.9 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 231 – 238 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- chromatin binding Source: MGI
- helicase activity Source: UniProtKB-KW
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cellular response to leukemia inhibitory factor Source: MGI
- chromatin silencing Source: MGI
- DNA methylation Source: MGI
- DNA methylation-dependent heterochromatin assembly Source: UniProtKB
- lymphocyte differentiation Source: MGI
- lymphocyte proliferation Source: UniProtKB
- maintenance of DNA methylation Source: UniProtKB
- multicellular organism development Source: UniProtKB
- negative regulation of apoptotic process Source: MGI
- negative regulation of intrinsic apoptotic signaling pathway Source: MGI
- pericentric heterochromatin assembly Source: UniProtKB
- urogenital system development Source: MGI
Keywordsi
Molecular function | Developmental protein, Helicase, Hydrolase |
Biological process | Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Lymphocyte-specific helicase (EC:3.6.4.-)Alternative name(s): Proliferation-associated SNF2-like protein |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:106209, Hells |
Subcellular locationi
Nucleus
- Nucleus 3 Publications
Note: Closely associated with pericentric heterochromatin.
Nucleus
- nucleus Source: MGI
Other locations
- chromosome, centromeric region Source: UniProtKB
- pericentric heterochromatin Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000260052 | 1 – 821 | Lymphocyte-specific helicaseAdd BLAST | 821 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 486 | PhosphoserineBy similarity | 1 | |
Modified residuei | 498 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q60848 |
jPOSTi | Q60848 |
PaxDbi | Q60848 |
PeptideAtlasi | Q60848 |
PRIDEi | Q60848 |
PTM databases
iPTMneti | Q60848 |
PhosphoSitePlusi | Q60848 |
Expressioni
Tissue specificityi
Highly expressed in thymus, bone marrow, and testis. Not detected in heart, brain, liver, kidney, skeletal muscle, spleen, peripheral blood lymphocytes, small intestine, colon, prostate or ovary.3 Publications
Developmental stagei
Differentially expressed during embryonic development, with highest expression in developing face, limbs, skeletal muscle, heart, and tail. Highly expressed in fetal thymocytes from day 14 to 16 of gestation, and expressed at much lower levels in adult thymus.3 Publications
Inductioni
By concanavalin or lipopolysaccharide in unactivated splenocytes.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000025001, Expressed in embryonic stem cell and 249 other tissues |
ExpressionAtlasi | Q60848, baseline and differential |
Genevisiblei | Q60848, MM |
Interactioni
Binary interactionsi
Hide detailsQ60848
Protein-protein interaction databases
BioGRIDi | 200271, 10 interactors |
DIPi | DIP-43735N |
IntActi | Q60848, 7 interactors |
MINTi | Q60848 |
STRINGi | 10090.ENSMUSP00000025965 |
Miscellaneous databases
RNActi | Q60848, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 218 – 386 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 169 | |
Domaini | 586 – 736 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 151 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 14 – 96 | Sequence analysisAdd BLAST | 83 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 337 – 340 | DEAH boxSequence analysis | 4 |
Sequence similaritiesi
Belongs to the SNF2/RAD54 helicase family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0385, Eukaryota |
GeneTreei | ENSGT00740000115593 |
HOGENOMi | CLU_000315_17_3_1 |
InParanoidi | Q60848 |
OMAi | LFESWFS |
OrthoDBi | 61251at2759 |
PhylomeDBi | Q60848 |
TreeFami | TF329077 |
Family and domain databases
Gene3Di | 3.40.50.10810, 1 hit |
InterProi | View protein in InterPro IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR038718, SNF2-like_sf IPR000330, SNF2_N |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF00176, SNF2_N, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 12 Publications (identifier: Q60848-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAEQTEPAVI TPAMLEEEEQ LEAAGLEKER KMLEEAQKSW DRESTEIRYR
60 70 80 90 100
RLQHLLEKSN IYSKFLLTKM EQQQLEEQKK KEKLEKKKRS LKLTEGKSLV
110 120 130 140 150
DGNGEKPVMK KKRGREDESY NISEVMSKEE ILSVAKKHKD NEDESSSTTS
160 170 180 190 200
LCVEDIQKNK DSNSMIKDRL SQTVRQNSKF FFDPVRKCNG QPVPFQQPKH
210 220 230 240 250
FTGGVMRWYQ VEGMEWLRML WENGINGILA DEMGLGKTVQ CIATIALMIQ
260 270 280 290 300
RGVPGPFLVC GPLSTLPNWM AEFKRFTPEI PTLLYHGTRE DRRKLVKNIH
310 320 330 340 350
KRQGTLQIHP VVVTSFEIAM RDQNALQHCY WKYLIVDEGH RIKNMKCRLI
360 370 380 390 400
RELKRFNADN KLLLTGTPLQ NNLSELWSLL NFLLPDVFDD LKSFESWFDI
410 420 430 440 450
TSLSETAEDI IAKEREQNVL HMLHQILTPF LLRRLKSDVA LEVPPKREVV
460 470 480 490 500
VYAPLCNKQE IFYTAIVNRT IANMFGSCEK ETVELSPTGR PKRRSRKSIN
510 520 530 540 550
YSELDQFPSE LEKLISQIQP EVNRERTVVE GNIPIESEVN LKLRNIMMLL
560 570 580 590 600
RKCCNHPYMI EYPIDPVTQE FKIDEELVTN SGKFLILDRM LPELKKRGHK
610 620 630 640 650
VLVFSQMTSM LDILMDYCHL RNFIFSRLDG SMSYSEREKN IYSFNTDPDV
660 670 680 690 700
FLFLVSTRAG GLGINLTAAD TVIIYDSDWN PQSDLQAQDR CHRIGQTKPV
710 720 730 740 750
VVYRLVTANT IDQKIVERAA AKRKLEKLII HKNHFKGGQS GLSQSKNFLD
760 770 780 790 800
AKELMELLKS RDYEREVKGS REKVISDEDL ELLLDRSDLI DQMKASRPIK
810 820
GKTGIFKILE NSEDSSAECL F
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3Z414 | D3Z414_MOUSE | Lymphocyte-specific helicase | Hells | 209 | Annotation score: |
Sequence cautioni
The sequence AAH20056 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 497 | K → R in AAI00395 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 620 | L → P in AAI00395 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_052235 | 1 – 13 | Missing in isoform 2. 1 PublicationAdd BLAST | 13 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25691 mRNA Translation: AAB08015.2 AF155210 mRNA Translation: AAG43373.1 AK013266 mRNA Translation: BAB28757.2 AK147126 mRNA Translation: BAE27697.1 BC020056 mRNA Translation: AAH20056.1 Different initiation. BC100394 mRNA Translation: AAI00395.1 |
CCDSi | CCDS29789.1 [Q60848-1] |
PIRi | JC4666 |
RefSeqi | NP_032260.2, NM_008234.3 [Q60848-1] XP_006526762.1, XM_006526699.3 [Q60848-1] |
Genome annotation databases
Ensembli | ENSMUST00000025965; ENSMUSP00000025965; ENSMUSG00000025001 [Q60848-1] |
GeneIDi | 15201 |
KEGGi | mmu:15201 |
UCSCi | uc008hjt.1, mouse [Q60848-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25691 mRNA Translation: AAB08015.2 AF155210 mRNA Translation: AAG43373.1 AK013266 mRNA Translation: BAB28757.2 AK147126 mRNA Translation: BAE27697.1 BC020056 mRNA Translation: AAH20056.1 Different initiation. BC100394 mRNA Translation: AAI00395.1 |
CCDSi | CCDS29789.1 [Q60848-1] |
PIRi | JC4666 |
RefSeqi | NP_032260.2, NM_008234.3 [Q60848-1] XP_006526762.1, XM_006526699.3 [Q60848-1] |
3D structure databases
SMRi | Q60848 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200271, 10 interactors |
DIPi | DIP-43735N |
IntActi | Q60848, 7 interactors |
MINTi | Q60848 |
STRINGi | 10090.ENSMUSP00000025965 |
PTM databases
iPTMneti | Q60848 |
PhosphoSitePlusi | Q60848 |
Proteomic databases
EPDi | Q60848 |
jPOSTi | Q60848 |
PaxDbi | Q60848 |
PeptideAtlasi | Q60848 |
PRIDEi | Q60848 |
Protocols and materials databases
Antibodypediai | 3852, 259 antibodies |
DNASUi | 15201 |
Genome annotation databases
Ensembli | ENSMUST00000025965; ENSMUSP00000025965; ENSMUSG00000025001 [Q60848-1] |
GeneIDi | 15201 |
KEGGi | mmu:15201 |
UCSCi | uc008hjt.1, mouse [Q60848-1] |
Organism-specific databases
CTDi | 3070 |
MGIi | MGI:106209, Hells |
Phylogenomic databases
eggNOGi | KOG0385, Eukaryota |
GeneTreei | ENSGT00740000115593 |
HOGENOMi | CLU_000315_17_3_1 |
InParanoidi | Q60848 |
OMAi | LFESWFS |
OrthoDBi | 61251at2759 |
PhylomeDBi | Q60848 |
TreeFami | TF329077 |
Miscellaneous databases
BioGRID-ORCSi | 15201, 3 hits in 19 CRISPR screens |
ChiTaRSi | Hells, mouse |
PROi | PR:Q60848 |
RNActi | Q60848, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025001, Expressed in embryonic stem cell and 249 other tissues |
ExpressionAtlasi | Q60848, baseline and differential |
Genevisiblei | Q60848, MM |
Family and domain databases
Gene3Di | 3.40.50.10810, 1 hit |
InterProi | View protein in InterPro IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR038718, SNF2-like_sf IPR000330, SNF2_N |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF00176, SNF2_N, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HELLS_MOUSE | |
Accessioni | Q60848Primary (citable) accession number: Q60848 Secondary accession number(s): Q497T3, Q8VDZ1, Q9CYV7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 157 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families