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Protein

Lymphocyte-specific helicase

Gene

Hells

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis.9 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi231 – 238ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chromatin binding Source: MGI
  • helicase activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Helicase, Hydrolase
Biological processCell cycle, Cell division, Mitosis, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lymphocyte-specific helicase (EC:3.6.4.-)
Alternative name(s):
Proliferation-associated SNF2-like protein
Gene namesi
Name:HellsImported
Synonyms:Lsh1 Publication, PasgImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:106209 Hells

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002600521 – 821Lymphocyte-specific helicaseAdd BLAST821

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei486PhosphoserineBy similarity1
Modified residuei498PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ60848
PeptideAtlasiQ60848
PRIDEiQ60848

PTM databases

iPTMnetiQ60848
PhosphoSitePlusiQ60848

Expressioni

Tissue specificityi

Highly expressed in thymus, bone marrow, and testis. Not detected in heart, brain, liver, kidney, skeletal muscle, spleen, peripheral blood lymphocytes, small intestine, colon, prostate or ovary.3 Publications

Developmental stagei

Differentially expressed during embryonic development, with highest expression in developing face, limbs, skeletal muscle, heart, and tail. Highly expressed in fetal thymocytes from day 14 to 16 of gestation, and expressed at much lower levels in adult thymus.3 Publications

Inductioni

By concanavalin or lipopolysaccharide in unactivated splenocytes.2 Publications

Gene expression databases

BgeeiENSMUSG00000025001 Expressed in 232 organ(s), highest expression level in embryonic stem cell
CleanExiMM_HELLS
ExpressionAtlasiQ60848 baseline and differential
GenevisibleiQ60848 MM

Interactioni

Binary interactionsi

Protein-protein interaction databases

BioGridi200271, 6 interactors
DIPiDIP-43735N
IntActiQ60848, 7 interactors
MINTiQ60848
STRINGi10090.ENSMUSP00000025965

Structurei

3D structure databases

ProteinModelPortaliQ60848
SMRiQ60848
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini218 – 386Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST169
Domaini586 – 736Helicase C-terminalPROSITE-ProRule annotationAdd BLAST151

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili14 – 96Sequence analysisAdd BLAST83

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi337 – 340DEAH boxSequence analysis4

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0386 Eukaryota
COG0553 LUCA
GeneTreeiENSGT00740000115593
HOGENOMiHOG000172362
HOVERGENiHBG060049
InParanoidiQ60848
KOiK19001
OMAiKSFESWF
OrthoDBiEOG091G03LZ
PhylomeDBiQ60848
TreeFamiTF329077

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di3.40.50.10810, 1 hit
InterProiView protein in InterPro
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR038718 SNF2-like_sf
IPR000330 SNF2_N
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF00176 SNF2_N, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 12 Publications (identifier: Q60848-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAEQTEPAVI TPAMLEEEEQ LEAAGLEKER KMLEEAQKSW DRESTEIRYR
60 70 80 90 100
RLQHLLEKSN IYSKFLLTKM EQQQLEEQKK KEKLEKKKRS LKLTEGKSLV
110 120 130 140 150
DGNGEKPVMK KKRGREDESY NISEVMSKEE ILSVAKKHKD NEDESSSTTS
160 170 180 190 200
LCVEDIQKNK DSNSMIKDRL SQTVRQNSKF FFDPVRKCNG QPVPFQQPKH
210 220 230 240 250
FTGGVMRWYQ VEGMEWLRML WENGINGILA DEMGLGKTVQ CIATIALMIQ
260 270 280 290 300
RGVPGPFLVC GPLSTLPNWM AEFKRFTPEI PTLLYHGTRE DRRKLVKNIH
310 320 330 340 350
KRQGTLQIHP VVVTSFEIAM RDQNALQHCY WKYLIVDEGH RIKNMKCRLI
360 370 380 390 400
RELKRFNADN KLLLTGTPLQ NNLSELWSLL NFLLPDVFDD LKSFESWFDI
410 420 430 440 450
TSLSETAEDI IAKEREQNVL HMLHQILTPF LLRRLKSDVA LEVPPKREVV
460 470 480 490 500
VYAPLCNKQE IFYTAIVNRT IANMFGSCEK ETVELSPTGR PKRRSRKSIN
510 520 530 540 550
YSELDQFPSE LEKLISQIQP EVNRERTVVE GNIPIESEVN LKLRNIMMLL
560 570 580 590 600
RKCCNHPYMI EYPIDPVTQE FKIDEELVTN SGKFLILDRM LPELKKRGHK
610 620 630 640 650
VLVFSQMTSM LDILMDYCHL RNFIFSRLDG SMSYSEREKN IYSFNTDPDV
660 670 680 690 700
FLFLVSTRAG GLGINLTAAD TVIIYDSDWN PQSDLQAQDR CHRIGQTKPV
710 720 730 740 750
VVYRLVTANT IDQKIVERAA AKRKLEKLII HKNHFKGGQS GLSQSKNFLD
760 770 780 790 800
AKELMELLKS RDYEREVKGS REKVISDEDL ELLLDRSDLI DQMKASRPIK
810 820
GKTGIFKILE NSEDSSAECL F
Length:821
Mass (Da):95,126
Last modified:March 1, 2001 - v2
Checksum:i8DEC2F2EEFBBD2F5
GO
Isoform 21 Publication (identifier: Q60848-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:808
Mass (Da):93,786
Checksum:iDBF5E847C1209568
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z414D3Z414_MOUSE
Lymphocyte-specific helicase
Hells
209Annotation score:

Sequence cautioni

The sequence AAH20056 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti497K → R in AAI00395 (PubMed:15489334).Curated1
Sequence conflicti620L → P in AAI00395 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0522351 – 13Missing in isoform 2. 1 PublicationAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25691 mRNA Translation: AAB08015.2
AF155210 mRNA Translation: AAG43373.1
AK013266 mRNA Translation: BAB28757.2
AK147126 mRNA Translation: BAE27697.1
BC020056 mRNA Translation: AAH20056.1 Different initiation.
BC100394 mRNA Translation: AAI00395.1
CCDSiCCDS29789.1 [Q60848-1]
PIRiJC4666
RefSeqiNP_032260.2, NM_008234.3 [Q60848-1]
XP_006526762.1, XM_006526699.3 [Q60848-1]
UniGeneiMm.392920
Mm.486446
Mm.57223

Genome annotation databases

EnsembliENSMUST00000025965; ENSMUSP00000025965; ENSMUSG00000025001 [Q60848-1]
GeneIDi15201
KEGGimmu:15201
UCSCiuc008hjt.1 mouse [Q60848-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25691 mRNA Translation: AAB08015.2
AF155210 mRNA Translation: AAG43373.1
AK013266 mRNA Translation: BAB28757.2
AK147126 mRNA Translation: BAE27697.1
BC020056 mRNA Translation: AAH20056.1 Different initiation.
BC100394 mRNA Translation: AAI00395.1
CCDSiCCDS29789.1 [Q60848-1]
PIRiJC4666
RefSeqiNP_032260.2, NM_008234.3 [Q60848-1]
XP_006526762.1, XM_006526699.3 [Q60848-1]
UniGeneiMm.392920
Mm.486446
Mm.57223

3D structure databases

ProteinModelPortaliQ60848
SMRiQ60848
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200271, 6 interactors
DIPiDIP-43735N
IntActiQ60848, 7 interactors
MINTiQ60848
STRINGi10090.ENSMUSP00000025965

PTM databases

iPTMnetiQ60848
PhosphoSitePlusiQ60848

Proteomic databases

PaxDbiQ60848
PeptideAtlasiQ60848
PRIDEiQ60848

Protocols and materials databases

DNASUi15201
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025965; ENSMUSP00000025965; ENSMUSG00000025001 [Q60848-1]
GeneIDi15201
KEGGimmu:15201
UCSCiuc008hjt.1 mouse [Q60848-1]

Organism-specific databases

CTDi3070
MGIiMGI:106209 Hells

Phylogenomic databases

eggNOGiKOG0386 Eukaryota
COG0553 LUCA
GeneTreeiENSGT00740000115593
HOGENOMiHOG000172362
HOVERGENiHBG060049
InParanoidiQ60848
KOiK19001
OMAiKSFESWF
OrthoDBiEOG091G03LZ
PhylomeDBiQ60848
TreeFamiTF329077

Miscellaneous databases

PROiPR:Q60848
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025001 Expressed in 232 organ(s), highest expression level in embryonic stem cell
CleanExiMM_HELLS
ExpressionAtlasiQ60848 baseline and differential
GenevisibleiQ60848 MM

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di3.40.50.10810, 1 hit
InterProiView protein in InterPro
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR038718 SNF2-like_sf
IPR000330 SNF2_N
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF00176 SNF2_N, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHELLS_MOUSE
AccessioniPrimary (citable) accession number: Q60848
Secondary accession number(s): Q497T3, Q8VDZ1, Q9CYV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: March 1, 2001
Last modified: September 12, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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