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UniProtKB - Q60843 (KLF2_MOUSE)
Protein
Krueppel-like factor 2
Gene
Klf2
Organism
Mus musculus (Mouse)
Status
Functioni
Transcription factor that binds to the CACCC box in the promoter of target genes such as HBB/beta globin or NOV and activates their transcription. Might be involved in transcriptional regulation by modulating the binding of the RARA nuclear receptor to RARE DNA elements (By similarity).
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 271 – 295 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 301 – 325 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 331 – 353 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- cell morphogenesis Source: MGI
- cellular response to cycloheximide Source: Ensembl
- cellular response to fluid shear stress Source: MGI
- cellular response to hydrogen peroxide Source: Ensembl
- cellular response to interleukin-1 Source: Ensembl
- cellular response to laminar fluid shear stress Source: MGI
- cellular response to peptide Source: Ensembl
- cellular response to tumor necrosis factor Source: Ensembl
- cellular stress response to acid chemical Source: MGI
- erythrocyte homeostasis Source: MGI
- erythrocyte maturation Source: MGI
- in utero embryonic development Source: MGI
- multicellular organism growth Source: MGI
- negative regulation of interleukin-6 production Source: MGI
- negative regulation of sprouting angiogenesis Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of nitric oxide biosynthetic process Source: MGI
- positive regulation of protein metabolic process Source: MGI
- positive regulation of retinoic acid receptor signaling pathway Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of transcription from RNA polymerase II promoter in response to stress Source: MGI
- regulation of gene expression, epigenetic Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- type I pneumocyte differentiation Source: MGI
- vasodilation Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Krueppel-like factor 2Alternative name(s): Lung krueppel-like factor |
Gene namesi | Name:Klf2 Synonyms:Lklf |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1342772, Klf2 |
VEuPathDBi | HostDB:ENSMUSG00000055148 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 121 | K → R: Greatly impairs polyubiquitination. Abolishes polyubiquitination; when associated with R-146. 1 Publication | 1 | |
Mutagenesisi | 146 | K → R: Abolishes polyubiquitination; when associated with R-121. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047163 | 1 – 354 | Krueppel-like factor 2Add BLAST | 354 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 171 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 243 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 246 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.1 Publication
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q60843 |
MaxQBi | Q60843 |
PaxDbi | Q60843 |
PRIDEi | Q60843 |
ProteomicsDBi | 263619 |
PTM databases
iPTMneti | Q60843 |
PhosphoSitePlusi | Q60843 |
Expressioni
Tissue specificityi
Predominant expression in the lungs and spleen.
Gene expression databases
Bgeei | ENSMUSG00000055148, Expressed in granulocyte and 258 other tissues |
Genevisiblei | Q60843, MM |
Interactioni
Subunit structurei
Interacts with WWP1.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 200964, 2 interactors |
STRINGi | 10090.ENSMUSP00000064823 |
Miscellaneous databases
RNActi | Q60843, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 52 – 111 | DisorderedSequence analysisAdd BLAST | 60 | |
Regioni | 110 – 267 | Interaction with WWP1Add BLAST | 158 | |
Regioni | 145 – 208 | DisorderedSequence analysisAdd BLAST | 64 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 42 – 50 | 9aaTADBy similarity | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 58 – 84 | Pro residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 161 – 208 | Pro residuesSequence analysisAdd BLAST | 48 |
Domaini
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.By similarity
Sequence similaritiesi
Belongs to the krueppel C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 271 – 295 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 301 – 325 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 331 – 353 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000163163 |
HOGENOMi | CLU_002678_33_1_1 |
InParanoidi | Q60843 |
OMAi | LHERWKC |
OrthoDBi | 1273088at2759 |
TreeFami | TF350556 |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
i Sequence
Sequence statusi: Complete.
Q60843-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALSEPILPS FATFASPCER GLQERWPRNE PEAGGTDEDL NNVLDFILSM
60 70 80 90 100
GLDGLGAENP PEPPPQPPPP AFYYPEPGAP PPYSIPAASL GTELLRPDLD
110 120 130 140 150
PPQGPALHGR FLLAPPGRLV KAEPPEVDGG GYGCAPGLAH GPRGLKLEGA
160 170 180 190 200
PGATGACMRG PAGRPPPPPD TPPLSPDGPL RIPASGPRNP FPPPFGPGPS
210 220 230 240 250
FGGPGPALHY GPPAPGAFGL FEDAAAAAAA LGLAPPATRG LLTPPSSPLE
260 270 280 290 300
LLEAKPKRGR RSWPRKRAAT HTCSYTNCGK TYTKSSHLKA HLRTHTGEKP
310 320 330 340 350
YHCNWEGCGW KFARSDELTR HYRKHTGHRP FQCHLCDRAF SRSDHLALHM
KRHM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 88 | A → D in AAA86728 (PubMed:7565748).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25096 mRNA Translation: AAA86728.1 BC138737 mRNA Translation: AAI38738.1 BC138738 mRNA Translation: AAI38739.1 |
CCDSi | CCDS22412.1 |
RefSeqi | NP_032478.2, NM_008452.2 |
Genome annotation databases
Ensembli | ENSMUST00000067912; ENSMUSP00000064823; ENSMUSG00000055148 |
GeneIDi | 16598 |
KEGGi | mmu:16598 |
UCSCi | uc009mfq.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25096 mRNA Translation: AAA86728.1 BC138737 mRNA Translation: AAI38738.1 BC138738 mRNA Translation: AAI38739.1 |
CCDSi | CCDS22412.1 |
RefSeqi | NP_032478.2, NM_008452.2 |
3D structure databases
AlphaFoldDBi | Q60843 |
SMRi | Q60843 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200964, 2 interactors |
STRINGi | 10090.ENSMUSP00000064823 |
PTM databases
iPTMneti | Q60843 |
PhosphoSitePlusi | Q60843 |
Proteomic databases
jPOSTi | Q60843 |
MaxQBi | Q60843 |
PaxDbi | Q60843 |
PRIDEi | Q60843 |
ProteomicsDBi | 263619 |
Protocols and materials databases
Antibodypediai | 27348, 442 antibodies from 32 providers |
DNASUi | 16598 |
Genome annotation databases
Ensembli | ENSMUST00000067912; ENSMUSP00000064823; ENSMUSG00000055148 |
GeneIDi | 16598 |
KEGGi | mmu:16598 |
UCSCi | uc009mfq.2, mouse |
Organism-specific databases
CTDi | 10365 |
MGIi | MGI:1342772, Klf2 |
VEuPathDBi | HostDB:ENSMUSG00000055148 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000163163 |
HOGENOMi | CLU_002678_33_1_1 |
InParanoidi | Q60843 |
OMAi | LHERWKC |
OrthoDBi | 1273088at2759 |
TreeFami | TF350556 |
Miscellaneous databases
BioGRID-ORCSi | 16598, 2 hits in 74 CRISPR screens |
ChiTaRSi | Klf2, mouse |
PROi | PR:Q60843 |
RNActi | Q60843, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000055148, Expressed in granulocyte and 258 other tissues |
Genevisiblei | Q60843, MM |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | KLF2_MOUSE | |
Accessioni | Q60843Primary (citable) accession number: Q60843 Secondary accession number(s): B2RS60 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | July 27, 2011 | |
Last modified: | May 25, 2022 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families