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Protein

CCR4-NOT transcription complex subunit 7

Gene

Cnot7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for miRNA-mediated mRNA deadenylation. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.2 Publications

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.

Cofactori

Mn2+By similarity, Mg2+By similarity, Co2+By similarityNote: Binds 2 divalent metal cations per subunit with RNAase activity being higher in presence of Mn2+ than of Mg2+ or Co2+.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Divalent metal cation 1; catalyticBy similarity1
Metal bindingi40Divalent metal cation 2; catalyticBy similarity1
Metal bindingi42Divalent metal cation 2; catalyticBy similarity1
Metal bindingi161Divalent metal cation 1; catalyticBy similarity1
Metal bindingi230Divalent metal cation 2; catalyticBy similarity1
Metal bindingi278Divalent metal cation 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease, Repressor, RNA-binding
Biological processRNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain

Names & Taxonomyi

Protein namesi
Recommended name:
CCR4-NOT transcription complex subunit 7 (EC:3.1.13.4)
Alternative name(s):
CCR4-associated factor 1
Short name:
CAF-1
Gene namesi
Name:Cnot7
Synonyms:Caf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1298230 Cnot7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002128451 – 285CCR4-NOT transcription complex subunit 7Add BLAST285

Proteomic databases

EPDiQ60809
MaxQBiQ60809
PaxDbiQ60809
PRIDEiQ60809

PTM databases

iPTMnetiQ60809
PhosphoSitePlusiQ60809

Expressioni

Developmental stagei

Expressed in embryonic stem (ES) cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000031601 Expressed in 305 organ(s), highest expression level in primary oocyte
CleanExiMM_CNOT7
ExpressionAtlasiQ60809 baseline and differential
GenevisibleiQ60809 MM

Interactioni

Subunit structurei

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits; the complex contains two deadenylase subunits, CNOT6 or CNOT6L, and CNOT7 or CNOT8 (By similarity). In the complex, interacts directly with CNOT1 (By similarity). Interacts with AGO2 (PubMed:19716330). Interacts with TOB1; recruited by TOB1 to a ternary complex with CPEB3 which is required for mRNA deadenylation and decay (By similarity). Interacts with BTG1 (PubMed:9712883). Interacts with BTG2 (PubMed:9712883). Interacts with NANOS2 (PubMed:20133598). Interacts with ZFP36, ZFP36L1 and ZFP36L2; these interactions are inhibited in response to phorbol 12-myristate 13-acetate (PMA) treatment in a p38 MAPK-dependent manner (By similarity).By similarity3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202298, 4 interactors
DIPiDIP-46843N
IntActiQ60809, 27 interactors
MINTiQ60809
STRINGi10090.ENSMUSP00000034012

Structurei

3D structure databases

ProteinModelPortaliQ60809
SMRiQ60809
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CAF1 family.Curated

Phylogenomic databases

eggNOGiKOG0304 Eukaryota
COG5228 LUCA
GeneTreeiENSGT00390000000080
HOGENOMiHOG000173077
HOVERGENiHBG051044
InParanoidiQ60809
KOiK12581
OMAiMPSNEEC
OrthoDBiEOG091G0M48
PhylomeDBiQ60809
TreeFamiTF314185

Family and domain databases

Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR039636 CNOT7
IPR039637 CNOT7/CNOT8/Pop2
IPR006941 RNase_CAF1
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PANTHERiPTHR10797 PTHR10797, 1 hit
PTHR10797:SF2 PTHR10797:SF2, 1 hit
PfamiView protein in Pfam
PF04857 CAF1, 2 hits
SUPFAMiSSF53098 SSF53098, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q60809-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPAATVDHSQ RICEVWACNL DEEMKKIRQV IRKYNYVAMD TEFPGVVARP
60 70 80 90 100
IGEFRSNADY QYQLLRCNVD LLKIIQLGLT FMNEQGEYPP GTSTWQFNFK
110 120 130 140 150
FNLTEDMYAQ DSIELLTTSG IQFKKHEEEG IETQYFAELL MTSGVVLCEG
160 170 180 190 200
VKWLSFHSGY DFGYLIKILT NSNLPEEELD FFEILRLFFP VIYDVKYLMK
210 220 230 240 250
SCKNLKGGLQ EVAEQLELER IGPQHQAGSD SLLTGMAFFK MREMFFEDHI
260 270 280
DDAKYCGHLY GLGSGSSYVQ NGTGNAYEEE ASKQS
Length:285
Mass (Da):32,718
Last modified:November 1, 1996 - v1
Checksum:i2AF23EC38E06EFDB
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3TLK9Q3TLK9_MOUSE
CCR4-NOT transcription complex subu...
Cnot7
248Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21855 mRNA Translation: AAA87455.1
BC006021 mRNA Translation: AAH06021.1
CCDSiCCDS22253.1
RefSeqiNP_001258471.1, NM_001271542.1
NP_035265.1, NM_011135.5
XP_011240485.1, XM_011242183.1
UniGeneiMm.272551

Genome annotation databases

EnsembliENSMUST00000034012; ENSMUSP00000034012; ENSMUSG00000031601
ENSMUST00000132032; ENSMUSP00000122933; ENSMUSG00000031601
ENSMUST00000149992; ENSMUSP00000117304; ENSMUSG00000031601
GeneIDi18983
KEGGimmu:18983
UCSCiuc009lmq.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21855 mRNA Translation: AAA87455.1
BC006021 mRNA Translation: AAH06021.1
CCDSiCCDS22253.1
RefSeqiNP_001258471.1, NM_001271542.1
NP_035265.1, NM_011135.5
XP_011240485.1, XM_011242183.1
UniGeneiMm.272551

3D structure databases

ProteinModelPortaliQ60809
SMRiQ60809
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202298, 4 interactors
DIPiDIP-46843N
IntActiQ60809, 27 interactors
MINTiQ60809
STRINGi10090.ENSMUSP00000034012

PTM databases

iPTMnetiQ60809
PhosphoSitePlusiQ60809

Proteomic databases

EPDiQ60809
MaxQBiQ60809
PaxDbiQ60809
PRIDEiQ60809

Protocols and materials databases

DNASUi18983
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034012; ENSMUSP00000034012; ENSMUSG00000031601
ENSMUST00000132032; ENSMUSP00000122933; ENSMUSG00000031601
ENSMUST00000149992; ENSMUSP00000117304; ENSMUSG00000031601
GeneIDi18983
KEGGimmu:18983
UCSCiuc009lmq.2 mouse

Organism-specific databases

CTDi29883
MGIiMGI:1298230 Cnot7

Phylogenomic databases

eggNOGiKOG0304 Eukaryota
COG5228 LUCA
GeneTreeiENSGT00390000000080
HOGENOMiHOG000173077
HOVERGENiHBG051044
InParanoidiQ60809
KOiK12581
OMAiMPSNEEC
OrthoDBiEOG091G0M48
PhylomeDBiQ60809
TreeFamiTF314185

Enzyme and pathway databases

ReactomeiR-MMU-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain

Miscellaneous databases

ChiTaRSiCnot7 mouse
PROiPR:Q60809
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031601 Expressed in 305 organ(s), highest expression level in primary oocyte
CleanExiMM_CNOT7
ExpressionAtlasiQ60809 baseline and differential
GenevisibleiQ60809 MM

Family and domain databases

Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR039636 CNOT7
IPR039637 CNOT7/CNOT8/Pop2
IPR006941 RNase_CAF1
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PANTHERiPTHR10797 PTHR10797, 1 hit
PTHR10797:SF2 PTHR10797:SF2, 1 hit
PfamiView protein in Pfam
PF04857 CAF1, 2 hits
SUPFAMiSSF53098 SSF53098, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCNOT7_MOUSE
AccessioniPrimary (citable) accession number: Q60809
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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