UniProtKB - Q60803 (TRAF3_MOUSE)
TNF receptor-associated factor 3
Traf3
Functioni
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 67 – 76 | RING-typePROSITE-ProRule annotation | 10 | |
Zinc fingeri | 134 – 189 | TRAF-type 1PROSITE-ProRule annotationAdd BLAST | 56 | |
Zinc fingeri | 190 – 248 | TRAF-type 2PROSITE-ProRule annotationAdd BLAST | 59 |
GO - Molecular functioni
- identical protein binding Source: MGI
- protein kinase binding Source: UniProtKB
- protein phosphatase binding Source: MGI
- thioesterase binding Source: MGI
- tumor necrosis factor receptor binding Source: MGI
- ubiquitin protein ligase binding Source: MGI
- ubiquitin-protein transferase activity Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- innate immune response Source: InterPro
- negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
- positive regulation of JNK cascade Source: GO_Central
- protein K63-linked ubiquitination Source: GO_Central
- regulation of apoptotic process Source: InterPro
- regulation of cytokine production Source: UniProtKB
- regulation of defense response to virus Source: UniProtKB
- regulation of I-kappaB kinase/NF-kappaB signaling Source: GO_Central
- regulation of interferon-beta production Source: UniProtKB
- regulation of proteolysis Source: UniProtKB
- toll-like receptor signaling pathway Source: UniProtKB
- Toll signaling pathway Source: InterPro
- tumor necrosis factor-mediated signaling pathway Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Apoptosis, Immunity, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-5668541, TNFR2 non-canonical NF-kB pathway R-MMU-5676594, TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway R-MMU-5689896, Ovarian tumor domain proteases R-MMU-936964, Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon |
Names & Taxonomyi
Protein namesi | Recommended name: TNF receptor-associated factor 3 (EC:2.3.2.27)Alternative name(s): CD40 receptor-associated factor 1 Short name: CRAF1 RING-type E3 ubiquitin transferase TRAF3Curated TRAFAMN |
Gene namesi | Name:Traf3 Synonyms:Craf1, Trafamn |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:108041, Traf3 |
Subcellular locationi
Endosome
- endosome Source: UniProtKB-SubCell
Plasma Membrane
- CD40 receptor complex Source: BHF-UCL
- cytoplasmic side of plasma membrane Source: BHF-UCL
Keywords - Cellular componenti
Cytoplasm, EndosomePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 52 | C → A: Abolishes inhibition of NFKB2 processing; when associated with A-55. 1 Publication | 1 | |
Mutagenesisi | 55 | C → A: Abolishes inhibition of NFKB2 processing; when associated with A-52. 1 Publication | 1 | |
Mutagenesisi | 67 | C → A: Strongly reduces 'Lys-63'-linked ubiquitination; when associated with A-69. Abolishes inhibition of NFKB2 processing; when associated with A-69. 2 Publications | 1 | |
Mutagenesisi | 69 | H → A: Strongly reduces 'Lys-63'-linked ubiquitination; when associated with A-69. Abolishes inhibition of NFKB2 processing; when associated with A-67. 2 Publications | 1 | |
Mutagenesisi | 106 | K → R: Reduces 'Lys-48'-linked polyubiquitination; when associated with R-155. | 1 | |
Mutagenesisi | 155 | K → R: Reduces 'Lys-48'-linked polyubiquitination; when associated with R-106. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056402 | 1 – 567 | TNF receptor-associated factor 3Add BLAST | 567 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 106 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Curated | ||
Cross-linki | 155 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Curated |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
EPDi | Q60803 |
MaxQBi | Q60803 |
PaxDbi | Q60803 |
PeptideAtlasi | Q60803 |
PRIDEi | Q60803 |
PTM databases
iPTMneti | Q60803 |
PhosphoSitePlusi | Q60803 |
SwissPalmi | Q60803 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000021277, Expressed in telencephalon and 305 other tissues |
ExpressionAtlasi | Q60803, baseline and differential |
Genevisiblei | Q60803, MM |
Interactioni
Subunit structurei
Homotrimer. Heterotrimer with TRAF2 and TRAF5.
Interacts with LTBR/TNFRSF3, TNFRSF4, TNFRSF5/CD40, TNFRSF8/CD30, TNFRSF13C TNFRSF17/BCMA, TLR4 and EDAR.
Interacts with MAP3K5, MAP3K14, TRAIP/TRIP, TDP2/TTRAP, TANK/ITRAF and TRAF3IP1. Interaction with TNFRSF5/CD40 is modulated by TANK/ITRAF, which competes for the same binding site.
Interacts with TICAM1.
Interacts with TRAFD1.
Interacts with OTUB1, OTUB2 and OTUD5.
Interacts with RNF216, OPTN and TBK1 (By similarity).
Identified in a complex with TRAF2, MAP3K14 and BIRC3. Upon exposure to bacterial lipopolysaccharide (LPS), recruited to a transient complex containing TLR4, TRAF3, TRAF6, IKBKG, MAP3K7, MYD88, TICAM1, BIRC2, BIRC3 and UBE2N.
Interacts (via RING-type zinc finger domain) with SRC.
Interacts with CARD14 (By similarity).
Interacts (via MATH domain) with PTPN22; the interaction promotes TRAF3 polyubiquitination (PubMed:23871208).
Interacts with MAVS (By similarity). Directly interacts with DDX3X; this interaction stimulates TRAF3 'Lys-63' ubiquitination (By similarity).
Interacts with IRF3 (By similarity).
Interacts with IKBKE in the course of viral infection (By similarity).
Interacts with TRIM35 (By similarity).
By similarity1 PublicationBinary interactionsi
Hide detailsQ60803
GO - Molecular functioni
- identical protein binding Source: MGI
- protein kinase binding Source: UniProtKB
- protein phosphatase binding Source: MGI
- thioesterase binding Source: MGI
- tumor necrosis factor receptor binding Source: MGI
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204304, 32 interactors |
DIPi | DIP-34050N |
IntActi | Q60803, 33 interactors |
MINTi | Q60803 |
STRINGi | 10090.ENSMUSP00000021706 |
Miscellaneous databases
RNActi | Q60803, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q60803 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 414 – 559 | MATHPROSITE-ProRule annotationAdd BLAST | 146 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 266 – 337 | Sequence analysisAdd BLAST | 72 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 67 – 76 | RING-typePROSITE-ProRule annotation | 10 | |
Zinc fingeri | 134 – 189 | TRAF-type 1PROSITE-ProRule annotationAdd BLAST | 56 | |
Zinc fingeri | 190 – 248 | TRAF-type 2PROSITE-ProRule annotationAdd BLAST | 59 |
Keywords - Domaini
Coiled coil, Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0297, Eukaryota |
GeneTreei | ENSGT00940000160538 |
InParanoidi | Q60803 |
OMAi | MVTLQKH |
OrthoDBi | 918518at2759 |
TreeFami | TF321154 |
Family and domain databases
CDDi | cd03777, MATH_TRAF3, 1 hit |
Gene3Di | 2.60.210.10, 1 hit 3.30.40.10, 2 hits |
InterProi | View protein in InterPro IPR002083, MATH/TRAF_dom IPR043211, TNF_rcpt-assoc_TRAF IPR012227, TNF_rcpt-assoc_TRAF_met IPR008974, TRAF-like IPR027128, TRAF3 IPR037304, TRAF3_MATH IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS IPR001293, Znf_TRAF |
PANTHERi | PTHR10131, PTHR10131, 1 hit PTHR10131:SF76, PTHR10131:SF76, 1 hit |
Pfami | View protein in Pfam PF02176, zf-TRAF, 1 hit |
PIRSFi | PIRSF015614, TRAF, 1 hit |
SMARTi | View protein in SMART SM00061, MATH, 1 hit |
SUPFAMi | SSF49599, SSF49599, 3 hits |
PROSITEi | View protein in PROSITE PS50144, MATH, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit PS50145, ZF_TRAF, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MESSKKMDAA GTLQPNPPLK LQPDRGAGSV LVPEQGGYKE KFVKTVEDKY
60 70 80 90 100
KCEKCRLVLC NPKQTECGHR FCESCMAALL SSSSPKCTAC QESIIKDKVF
110 120 130 140 150
KDNCCKREIL ALQVYCRNEG RGCAEQLTLG HLLVHLKNEC QFEELPCLRA
160 170 180 190 200
DCKEKVLRKD LRDHVEKACK YREATCSHCK SQVPMIKLQK HEDTDCPCVV
210 220 230 240 250
VSCPHKCSVQ TLLRSELSAH LSECVNAPST CSFKRYGCVF QGTNQQIKAH
260 270 280 290 300
EASSAVQHVN LLKEWSNSLE KKVSLLQNES VEKNKSIQSL HNQICSFEIE
310 320 330 340 350
IERQKEMLRN NESKILHLQR VIDSQAEKLK ELDKEIRPFR QNWEEADSMK
360 370 380 390 400
SSVESLQNRV TELESVDKSA GQAARNTGLL ESQLSRHDQM LSVHDIRLAD
410 420 430 440 450
MDLRFQVLET ASYNGVLIWK IRDYKRRKQE AVMGKTLSLY SQPFYTGYFG
460 470 480 490 500
YKMCARVYLN GDGMGKGTHL SLFFVIMRGE YDALLPWPFK QKVTLMLMDQ
510 520 530 540 550
GSSRRHLGDA FKPDPNSSSF KKPTGEMNIA SGCPVFVAQT VLENGTYIKD
560
DTIFIKVIVD TSDLPDP
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3UHJ1 | Q3UHJ1_MOUSE | TNF receptor-associated factor | Traf3 | 542 | Annotation score: | ||
D3Z343 | D3Z343_MOUSE | TNF receptor-associated factor 3 | Traf3 | 97 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 72 – 73 | CE → WQ in AAC52710 (PubMed:8660894).Curated | 2 | |
Sequence conflicti | 390 | M → T in AAC52175 (PubMed:7533327).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U21050 mRNA Translation: AAC52175.1 U33840 mRNA Translation: AAC52710.1 CH466549 Genomic DNA Translation: EDL18642.1 BC137634 mRNA Translation: AAI37635.1 BC137635 mRNA Translation: AAI37636.1 |
CCDSi | CCDS26175.1 |
PIRi | I49272 |
RefSeqi | NP_035762.2, NM_011632.3 XP_006515861.1, XM_006515798.2 XP_006515862.1, XM_006515799.1 XP_006515863.1, XM_006515800.2 XP_006515864.1, XM_006515801.2 |
Genome annotation databases
Ensembli | ENSMUST00000021706; ENSMUSP00000021706; ENSMUSG00000021277 |
GeneIDi | 22031 |
KEGGi | mmu:22031 |
UCSCi | uc007pcl.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U21050 mRNA Translation: AAC52175.1 U33840 mRNA Translation: AAC52710.1 CH466549 Genomic DNA Translation: EDL18642.1 BC137634 mRNA Translation: AAI37635.1 BC137635 mRNA Translation: AAI37636.1 |
CCDSi | CCDS26175.1 |
PIRi | I49272 |
RefSeqi | NP_035762.2, NM_011632.3 XP_006515861.1, XM_006515798.2 XP_006515862.1, XM_006515799.1 XP_006515863.1, XM_006515800.2 XP_006515864.1, XM_006515801.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4GHU | X-ray | 2.20 | A | 376-567 | [»] | |
SMRi | Q60803 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204304, 32 interactors |
DIPi | DIP-34050N |
IntActi | Q60803, 33 interactors |
MINTi | Q60803 |
STRINGi | 10090.ENSMUSP00000021706 |
PTM databases
iPTMneti | Q60803 |
PhosphoSitePlusi | Q60803 |
SwissPalmi | Q60803 |
Proteomic databases
EPDi | Q60803 |
MaxQBi | Q60803 |
PaxDbi | Q60803 |
PeptideAtlasi | Q60803 |
PRIDEi | Q60803 |
Protocols and materials databases
Antibodypediai | 129, 515 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000021706; ENSMUSP00000021706; ENSMUSG00000021277 |
GeneIDi | 22031 |
KEGGi | mmu:22031 |
UCSCi | uc007pcl.2, mouse |
Organism-specific databases
CTDi | 7187 |
MGIi | MGI:108041, Traf3 |
Phylogenomic databases
eggNOGi | KOG0297, Eukaryota |
GeneTreei | ENSGT00940000160538 |
InParanoidi | Q60803 |
OMAi | MVTLQKH |
OrthoDBi | 918518at2759 |
TreeFami | TF321154 |
Enzyme and pathway databases
Reactomei | R-MMU-5668541, TNFR2 non-canonical NF-kB pathway R-MMU-5676594, TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway R-MMU-5689896, Ovarian tumor domain proteases R-MMU-936964, Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon |
Miscellaneous databases
BioGRID-ORCSi | 22031, 7 hits in 22 CRISPR screens |
ChiTaRSi | Traf3, mouse |
PROi | PR:Q60803 |
RNActi | Q60803, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021277, Expressed in telencephalon and 305 other tissues |
ExpressionAtlasi | Q60803, baseline and differential |
Genevisiblei | Q60803, MM |
Family and domain databases
CDDi | cd03777, MATH_TRAF3, 1 hit |
Gene3Di | 2.60.210.10, 1 hit 3.30.40.10, 2 hits |
InterProi | View protein in InterPro IPR002083, MATH/TRAF_dom IPR043211, TNF_rcpt-assoc_TRAF IPR012227, TNF_rcpt-assoc_TRAF_met IPR008974, TRAF-like IPR027128, TRAF3 IPR037304, TRAF3_MATH IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS IPR001293, Znf_TRAF |
PANTHERi | PTHR10131, PTHR10131, 1 hit PTHR10131:SF76, PTHR10131:SF76, 1 hit |
Pfami | View protein in Pfam PF02176, zf-TRAF, 1 hit |
PIRSFi | PIRSF015614, TRAF, 1 hit |
SMARTi | View protein in SMART SM00061, MATH, 1 hit |
SUPFAMi | SSF49599, SSF49599, 3 hits |
PROSITEi | View protein in PROSITE PS50144, MATH, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit PS50145, ZF_TRAF, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRAF3_MOUSE | |
Accessioni | Q60803Primary (citable) accession number: Q60803 Secondary accession number(s): B2RPW3, Q62380 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 190 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families