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Protein

Krueppel-like factor 4

Gene

Klf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription (By similarity).By similarity7 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri400 – 424C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri430 – 454C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri460 – 482C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Krueppel-like factor 4
Alternative name(s):
Epithelial zinc finger protein EZF
Gut-enriched krueppel-like factor
Gene namesi
Name:Klf4
Synonyms:Ezf, Gklf, Zie
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1342287 Klf4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Biotechnological usei

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.2 Publications

Disruption phenotypei

Death shortly after birth due to loss of epidermal barrier function resulting from perturbation of late-stage epidermal differentiation structures including the cornified envelope. When selectively deleted in the surface ectoderm-derived structures of the eye, embryos develop normally and adults are viable and fertile but mutants display down-regulation of Krt12 and Aqp5 and multiple ocular defects including corneal epithelial fragility, stromal edema, defective lens and loss of conjunctival goblet cells.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000471681 – 483Krueppel-like factor 4Add BLAST483

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei251PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ60793
PaxDbiQ60793
PRIDEiQ60793

PTM databases

iPTMnetiQ60793
PhosphoSitePlusiQ60793

Expressioni

Tissue specificityi

Highest expression in the colon. Lower levels in testis, lung and small intestine.

Inductioni

By interferon-gamma in Stat1-dependent manner.1 Publication

Gene expression databases

BgeeiENSMUSG00000003032 Expressed in 291 organ(s), highest expression level in cornea
CleanExiMM_KLF4
ExpressionAtlasiQ60793 baseline and differential
GenevisibleiQ60793 MM

Interactioni

Subunit structurei

Interacts with MUC1 (via the C-terminal domain) (By similarity). Interacts with POU5F1/OCT4 and SOX2 (PubMed:19816951). Interacts with MEIS2 isoform MeisD and PBX1 isoform PBX1a (By similarity). Interacts with ZNF296 (PubMed:24161396).By similarity2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200966, 5 interactors
DIPiDIP-59920N
ELMiQ60793
IntActiQ60793, 12 interactors
STRINGi10090.ENSMUSP00000103245

Structurei

Secondary structure

1483
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ60793
SMRiQ60793
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60793

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni386 – 483Interaction with ZNF2961 PublicationAdd BLAST98
Regioni443 – 474Interaction with target DNAAdd BLAST32

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi176 – 385Pro-richAdd BLAST210

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri400 – 424C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri430 – 454C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri460 – 482C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00760000118998
HOGENOMiHOG000060173
HOVERGENiHBG006220
InParanoidiQ60793
KOiK17846
OMAiCTVGRPL
OrthoDBiEOG091G1BN0
PhylomeDBiQ60793
TreeFamiTF350556

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q60793-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRQPPGESDM AVSDALLPSF STFASGPAGR EKTLRPAGAP TNRWREELSH
60 70 80 90 100
MKRLPPLPGR PYDLAATVAT DLESGGAGAA CSSNNPALLA RRETEEFNDL
110 120 130 140 150
LDLDFILSNS LTHQESVAAT VTTSASASSS SSPASSGPAS APSTCSFSYP
160 170 180 190 200
IRAGGDPGVA ASNTGGGLLY SRESAPPPTA PFNLADINDV SPSGGFVAEL
210 220 230 240 250
LRPELDPVYI PPQQPQPPGG GLMGKFVLKA SLTTPGSEYS SPSVISVSKG
260 270 280 290 300
SPDGSHPVVV APYSGGPPRM CPKIKQEAVP SCTVSRSLEA HLSAGPQLSN
310 320 330 340 350
GHRPNTHDFP LGRQLPTRTT PTLSPEELLN SRDCHPGLPL PPGFHPHPGP
360 370 380 390 400
NYPPFLPDQM QSQVPSLHYQ ELMPPGSCLP EEPKPKRGRR SWPRKRTATH
410 420 430 440 450
TCDYAGCGKT YTKSSHLKAH LRTHTGEKPY HCDWDGCGWK FARSDELTRH
460 470 480
YRKHTGHRPF QCQKCDRAFS RSDHLALHMK RHF
Length:483
Mass (Da):51,880
Last modified:February 10, 2009 - v3
Checksum:i5D1B0BF7948C49B3
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7ZCH2B7ZCH2_MOUSE
Krueppel-like factor 4
Klf4
160Annotation score:
B7ZCH1B7ZCH1_MOUSE
Krueppel-like factor 4
Klf4
82Annotation score:

Sequence cautioni

The sequence AAC52939 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH10301 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162S → R in AAC52939 (PubMed:8940147).Curated1
Sequence conflicti185A → G in AAC52939 (PubMed:8940147).Curated1
Sequence conflicti302H → Q in BAE33205 (PubMed:16141072).Curated1
Sequence conflicti350P → A in AAC52939 (PubMed:8940147).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20344 mRNA Translation: AAC04892.1
U70662 mRNA Translation: AAC52939.1 Different initiation.
AF117109 Genomic DNA Translation: AAD17223.1
AY071827 Genomic DNA Translation: AAL60058.1
AK141244 mRNA Translation: BAE24612.1
AK144942 mRNA Translation: BAE26147.1
AK155343 mRNA Translation: BAE33205.1
AL732494 Genomic DNA Translation: CAM20848.1
CH466565 Genomic DNA Translation: EDL02262.1
BC010301 mRNA Translation: AAH10301.2 Different initiation.
CCDSiCCDS18195.2
RefSeqiNP_034767.2, NM_010637.3
UniGeneiMm.4325

Genome annotation databases

EnsembliENSMUST00000107619; ENSMUSP00000103245; ENSMUSG00000003032
GeneIDi16600
KEGGimmu:16600
UCSCiuc008sxk.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20344 mRNA Translation: AAC04892.1
U70662 mRNA Translation: AAC52939.1 Different initiation.
AF117109 Genomic DNA Translation: AAD17223.1
AY071827 Genomic DNA Translation: AAL60058.1
AK141244 mRNA Translation: BAE24612.1
AK144942 mRNA Translation: BAE26147.1
AK155343 mRNA Translation: BAE33205.1
AL732494 Genomic DNA Translation: CAM20848.1
CH466565 Genomic DNA Translation: EDL02262.1
BC010301 mRNA Translation: AAH10301.2 Different initiation.
CCDSiCCDS18195.2
RefSeqiNP_034767.2, NM_010637.3
UniGeneiMm.4325

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WBSX-ray1.70A395-483[»]
2WBUX-ray2.50A396-483[»]
4M9EX-ray1.85A396-483[»]
5KE6X-ray1.99A396-483[»]
5KE7X-ray2.06A396-483[»]
5KE8X-ray2.45A396-483[»]
5KE9X-ray2.34A396-483[»]
5KEAX-ray2.46A396-483[»]
5KEBX-ray2.45A396-483[»]
ProteinModelPortaliQ60793
SMRiQ60793
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200966, 5 interactors
DIPiDIP-59920N
ELMiQ60793
IntActiQ60793, 12 interactors
STRINGi10090.ENSMUSP00000103245

PTM databases

iPTMnetiQ60793
PhosphoSitePlusiQ60793

Proteomic databases

MaxQBiQ60793
PaxDbiQ60793
PRIDEiQ60793

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000107619; ENSMUSP00000103245; ENSMUSG00000003032
GeneIDi16600
KEGGimmu:16600
UCSCiuc008sxk.2 mouse

Organism-specific databases

CTDi9314
MGIiMGI:1342287 Klf4

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00760000118998
HOGENOMiHOG000060173
HOVERGENiHBG006220
InParanoidiQ60793
KOiK17846
OMAiCTVGRPL
OrthoDBiEOG091G1BN0
PhylomeDBiQ60793
TreeFamiTF350556

Miscellaneous databases

EvolutionaryTraceiQ60793
PROiPR:Q60793
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003032 Expressed in 291 organ(s), highest expression level in cornea
CleanExiMM_KLF4
ExpressionAtlasiQ60793 baseline and differential
GenevisibleiQ60793 MM

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiKLF4_MOUSE
AccessioniPrimary (citable) accession number: Q60793
Secondary accession number(s): P70421
, Q3U2D6, Q3URS6, Q78K30, Q9R255
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 10, 2009
Last modified: November 7, 2018
This is version 170 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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