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Protein

NF-kappa-B inhibitor beta

Gene

Nfkbib

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-1169091 Activation of NF-kappaB in B cells
R-MMU-1810476 RIP-mediated NFkB activation via ZBP1
R-MMU-445989 TAK1 activates NFkB by phosphorylation and activation of IKKs complex
R-MMU-933542 TRAF6 mediated NF-kB activation

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B inhibitor beta
Short name:
NF-kappa-BIB
Alternative name(s):
I-kappa-B-beta
Short name:
IkB-B
Short name:
IkB-beta
Short name:
IkappaBbeta
Gene namesi
Name:Nfkbib
Synonyms:Ikbb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:104752 Nfkbib

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670051 – 359NF-kappa-B inhibitor betaAdd BLAST359

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Phosphoserine; by RPS6KA1By similarity1
Modified residuei23Phosphoserine; by RPS6KA1By similarity1
Modified residuei313PhosphoserineBy similarity1
Modified residuei318PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ60778
MaxQBiQ60778
PaxDbiQ60778
PeptideAtlasiQ60778
PRIDEiQ60778

PTM databases

iPTMnetiQ60778
PhosphoSitePlusiQ60778

Expressioni

Tissue specificityi

Highly expressed in testis followed by spleen.

Gene expression databases

BgeeiENSMUSG00000030595 Expressed in 277 organ(s), highest expression level in testis
ExpressionAtlasiQ60778 baseline and differential
GenevisibleiQ60778 MM

Interactioni

Subunit structurei

Interacts with THRB (via ligand-binding domain) (By similarity). Interacts with RELA and REL (PubMed:8816457). Interacts with COMMD1 (By similarity). Interacts with inhibitor kappa B-interacting Ras-like NKIRAS1 and NKIRAS2 (PubMed:10657303).By similarity2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi201754, 3 interactors
DIPiDIP-37418N
IntActiQ60778, 4 interactors
MINTiQ60778
STRINGi10090.ENSMUSP00000032815

Structurei

Secondary structure

1359
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ60778
SMRiQ60778
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60778

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati57 – 86ANK 1Add BLAST30
Repeati93 – 122ANK 2Add BLAST30
Repeati126 – 155ANK 3Add BLAST30
Repeati206 – 235ANK 4Add BLAST30
Repeati240 – 269ANK 5Add BLAST30
Repeati273 – 302ANK 6Add BLAST30

Sequence similaritiesi

Belongs to the NF-kappa-B inhibitor family.Curated

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00550000074527
HOGENOMiHOG000137336
HOVERGENiHBG019039
InParanoidiQ60778
KOiK02581
OMAiENYEGHT
OrthoDBiEOG091G092R
TreeFamiTF320166

Family and domain databases

CDDicd00204 ANK, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 4 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q60778-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGVACLGKT ADADEWCDSG LGSLGPDAAA PGGPGLGAEL GPELSWAPLV
60 70 80 90 100
FGYVTEDGDT ALHLAVIHQH EPFLDFLLGF SAGTEYLDLQ NDLGQTALHL
110 120 130 140 150
AAILGEASTV EKLYAAGAGV LVAERGGHTA LHLACRVRAH TCACVLLQPR
160 170 180 190 200
PSHPRDASDT YLTQSQDCTP DTSHAPAAVD SQPNPENEEE PRDEDWRLQL
210 220 230 240 250
EAENYDGHTP LHVAVIHKDA EMVRLLRDAG ADLNKPEPTC GRTPLHLAVE
260 270 280 290 300
AQAASVLELL LKAGADPTAR MYGGRTPLGS ALLRPNPILA RLLRAHGAPE
310 320 330 340 350
PEDEDDKLSP CSSSGSDSDS DNRDEGDEYD DIVVHSGRSQ NRQPPSPASK

PLPDDPNPA
Length:359
Mass (Da):37,965
Last modified:July 27, 2011 - v2
Checksum:i0E2B46C89E2404EF
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z358D3Z358_MOUSE
NF-kappa-B inhibitor beta
Nfkbib
150Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84T → H in AAC52166 (PubMed:7867065).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19799 mRNA Translation: AAC52166.1
AK010218 mRNA Translation: BAB26774.1
AK038631 mRNA Translation: BAC30071.1
AK156731 mRNA Translation: BAE33825.1
CH466593 Genomic DNA Translation: EDL24117.1
CH466593 Genomic DNA Translation: EDL24118.1
CCDSiCCDS21054.1
RefSeqiNP_001293151.1, NM_001306222.1
NP_035038.2, NM_010908.5
UniGeneiMm.220333

Genome annotation databases

EnsembliENSMUST00000032815; ENSMUSP00000032815; ENSMUSG00000030595
ENSMUST00000085851; ENSMUSP00000083012; ENSMUSG00000030595
GeneIDi18036
KEGGimmu:18036
UCSCiuc009fzr.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19799 mRNA Translation: AAC52166.1
AK010218 mRNA Translation: BAB26774.1
AK038631 mRNA Translation: BAC30071.1
AK156731 mRNA Translation: BAE33825.1
CH466593 Genomic DNA Translation: EDL24117.1
CH466593 Genomic DNA Translation: EDL24118.1
CCDSiCCDS21054.1
RefSeqiNP_001293151.1, NM_001306222.1
NP_035038.2, NM_010908.5
UniGeneiMm.220333

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K3ZX-ray2.50D50-331[»]
1OY3X-ray2.05D50-331[»]
ProteinModelPortaliQ60778
SMRiQ60778
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201754, 3 interactors
DIPiDIP-37418N
IntActiQ60778, 4 interactors
MINTiQ60778
STRINGi10090.ENSMUSP00000032815

PTM databases

iPTMnetiQ60778
PhosphoSitePlusiQ60778

Proteomic databases

EPDiQ60778
MaxQBiQ60778
PaxDbiQ60778
PeptideAtlasiQ60778
PRIDEiQ60778

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032815; ENSMUSP00000032815; ENSMUSG00000030595
ENSMUST00000085851; ENSMUSP00000083012; ENSMUSG00000030595
GeneIDi18036
KEGGimmu:18036
UCSCiuc009fzr.1 mouse

Organism-specific databases

CTDi4793
MGIiMGI:104752 Nfkbib

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00550000074527
HOGENOMiHOG000137336
HOVERGENiHBG019039
InParanoidiQ60778
KOiK02581
OMAiENYEGHT
OrthoDBiEOG091G092R
TreeFamiTF320166

Enzyme and pathway databases

ReactomeiR-MMU-1169091 Activation of NF-kappaB in B cells
R-MMU-1810476 RIP-mediated NFkB activation via ZBP1
R-MMU-445989 TAK1 activates NFkB by phosphorylation and activation of IKKs complex
R-MMU-933542 TRAF6 mediated NF-kB activation

Miscellaneous databases

EvolutionaryTraceiQ60778
PROiPR:Q60778
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030595 Expressed in 277 organ(s), highest expression level in testis
ExpressionAtlasiQ60778 baseline and differential
GenevisibleiQ60778 MM

Family and domain databases

CDDicd00204 ANK, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiIKBB_MOUSE
AccessioniPrimary (citable) accession number: Q60778
Secondary accession number(s): Q564F1, Q9D6L5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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