UniProtKB - Q60766 (IRGM1_MOUSE)
Protein
Immunity-related GTPase family M protein 1
Gene
Irgm1
Organism
Mus musculus (Mouse)
Status
Functioni
Putative GTPase which is required for IFNG-mediated clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. Required for macrophage motility and possibly also for adhesion.9 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 84 – 91 | GTPBy similarity | 8 | |
Nucleotide bindingi | 109 – 113 | GTPBy similarity | 5 | |
Nucleotide bindingi | 191 – 193 | GTPBy similarity | 3 | |
Nucleotide bindingi | 232 – 234 | GTPBy similarity | 3 |
GO - Molecular functioni
- GTPase activity Source: GO_Central
- GTP binding Source: UniProtKB-KW
GO - Biological processi
- autophagosome assembly Source: UniProtKB
- cellular response to interferon-beta Source: GO_Central
- cellular response to interferon-gamma Source: MGI
- defense response Source: MGI
- positive regulation by symbiont of host autophagy Source: UniProtKB
- positive regulation of autophagosome maturation Source: UniProtKB
- positive regulation of interferon-gamma-mediated signaling pathway Source: UniProtKB
- response to bacterium Source: MGI
Keywordsi
Molecular function | Hydrolase |
Biological process | Autophagy, Immunity, Innate immunity |
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Immunity-related GTPase family M protein 1 (EC:3.6.5.-)Alternative name(s): Interferon-inducible GTPase 3 Interferon-inducible protein 1 LPS-stimulated RAW 264.7 macrophage protein 47 Short name: LRG-47 |
Gene namesi | Name:Irgm1 Synonyms:Ifi1, Iigp3, Irgm |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107567, Irgm1 |
Subcellular locationi
Lysosome
Endosome
Golgi apparatus
Plasma membrane
Other locations
Note: Behaves like an integral membrane protein. Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing phagosomes. Association with phagosomes is dependent on nucleotide-binding but is IFNG-independent. Preferentially associated with cis- and medial-Golgi. Also detected in late endosomes and lysosomes.
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum membrane Source: GO_Central
Endosome
- late endosome Source: UniProtKB
- late endosome membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Lysosome
- autolysosome Source: UniProtKB
- lysosomal membrane Source: UniProtKB-SubCell
- lysosome Source: UniProtKB
Plasma Membrane
- phagocytic cup Source: UniProtKB-SubCell
Other locations
- autophagosome Source: UniProtKB
- autophagosome membrane Source: UniProtKB-SubCell
- cell projection Source: UniProtKB-KW
- phagocytic vesicle Source: MGI
- phagocytic vesicle membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, Golgi apparatus, Lysosome, MembranePathology & Biotechi
Disruption phenotypei
Mice do not show obvious abnormalities except a loss of resistance to infection by S.typhimurium, T.cruzi, T.gondii, L.monocytogenes and M.tuberculosis. Upon infection, alterations of blood elements occur including lymphopenia, anemia, and thrombocytopenia.2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 90 | S → N: Loss of targeting to plasma membrane and phagosomes. 1 Publication | 1 | |
Mutagenesisi | 356 | C → A: No effect on subcellular location. 1 Publication | 1 | |
Mutagenesisi | 358 | I → A: No effect on subcellular location. 1 Publication | 1 | |
Mutagenesisi | 359 | V → A: No effect on subcellular location. 1 Publication | 1 | |
Mutagenesisi | 360 | N → A: No effect on subcellular location. Abolishes Golgi and lysosomal localization; when associated with A-364. | 1 | |
Mutagenesisi | 362 | F → A: No effect on subcellular location. 1 Publication | 1 | |
Mutagenesisi | 362 | F → EF: Disrupts amphipathicity and abolishes Golgi localization; when associated with Glu-367. 1 Publication | 1 | |
Mutagenesisi | 363 | F → A: No effect on subcellular location. 1 Publication | 1 | |
Mutagenesisi | 364 | R → A: No effect on subcellular location. Abolishes Golgi and lysosomal localization; when associated with A-360 or A-367. | 1 | |
Mutagenesisi | 365 | L → A: No effect on subcellular location. 1 Publication | 1 | |
Mutagenesisi | 366 | L → A: No effect on subcellular location. 1 Publication | 1 | |
Mutagenesisi | 367 | R → A: No effect on subcellular location. Abolishes Golgi and lysosomal localization; when associated with A-364. | 1 | |
Mutagenesisi | 367 | R → ER: Disrupts amphipathicity and abolishes Golgi localization; when associated with Glu-362. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000325750 | 1 – 409 | Immunity-related GTPase family M protein 1Add BLAST | 409 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 202 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q60766 |
PaxDbi | Q60766 |
PeptideAtlasi | Q60766 |
PRIDEi | Q60766 |
ProteomicsDBi | 268995 [Q60766-1] 268996 [Q60766-2] |
PTM databases
iPTMneti | Q60766 |
PhosphoSitePlusi | Q60766 |
SwissPalmi | Q60766 |
Expressioni
Tissue specificityi
Expressed in lung and primary macrophages.1 Publication
Inductioni
Up-regulated by LPS and IFNG (at protein level). Up-regulated upon infection by various pathogens including T.cruzi, T.gondii, L.monocytogenes, M.tuberculosis and murine cytomegalovirus.7 Publications
Gene expression databases
Bgeei | ENSMUSG00000046879, Expressed in submandibular gland and 241 other tissues |
ExpressionAtlasi | Q60766, baseline and differential |
Genevisiblei | Q60766, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 200520, 2 interactors |
IntActi | Q60766, 2 interactors |
MINTi | Q60766 |
STRINGi | 10090.ENSMUSP00000050446 |
Miscellaneous databases
RNActi | Q60766, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 75 – 251 | IRG-type GPROSITE-ProRule annotationAdd BLAST | 177 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 75 – 289 | Mediates targeting to cell membrane and phagosomesAdd BLAST | 215 | |
Regioni | 350 – 374 | Mediates targeting to the GolgiAdd BLAST | 25 |
Sequence similaritiesi
Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.PROSITE-ProRule annotationCurated
Phylogenomic databases
eggNOGi | ENOG502QS9R, Eukaryota |
GeneTreei | ENSGT00950000183007 |
InParanoidi | Q60766 |
OrthoDBi | 688334at2759 |
PhylomeDBi | Q60766 |
TreeFami | TF331897 |
Family and domain databases
InterProi | View protein in InterPro IPR030385, G_IRG_dom IPR007743, Immunity-related_GTPase-like IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF05049, IIGP, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51716, G_IRG, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q60766-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKPSHSSCEA APLLPNMAET HYAPLSSAFP FVTSYQTGSS RLPEVSRSTE
60 70 80 90 100
RALREGKLLE LVYGIKETVA TLSQIPVSIF VTGDSGNGMS SFINALRVIG
110 120 130 140 150
HDEDASAPTG VVRTTKTRTE YSSSHFPNVV LWDLPGLGAT AQTVEDYVEE
160 170 180 190 200
MKFSTCDLFI IIASEQFSSN HVKLSKIIQS MGKRFYIVWT KLDRDLSTSV
210 220 230 240 250
LSEVRLLQNI QENIRENLQK EKVKYPPVFL VSSLDPLLYD FPKLRDTLHK
260 270 280 290 300
DLSNIRCCEP LKTLYGTYEK IVGDKVAVWK QRIANESLKN SLGVRDDDNM
310 320 330 340 350
GECLKVYRLI FGVDDESVQQ VAQSMGTVVM EYKDNMKSQN FYTLRREDWK
360 370 380 390 400
LRLMTCAIVN AFFRLLRFLP CVCCCLRRLR HKRMLFLVAQ DTKNILEKIL
RDSIFPPQI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 151 | M → V in BAE39622 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_032384 | 1 – 16 | Missing in isoform 2. 1 PublicationAdd BLAST | 16 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U19119 mRNA Translation: AAB48942.1 AK002545 mRNA Translation: BAB22176.1 AK167558 mRNA Translation: BAE39622.1 AK171743 mRNA Translation: BAE42645.1 AL645849 Genomic DNA No translation available. BC145957 mRNA Translation: AAI45958.1 |
CCDSi | CCDS24588.1 [Q60766-1] CCDS88149.1 [Q60766-2] |
RefSeqi | NP_032352.1, NM_008326.1 [Q60766-1] |
Genome annotation databases
Ensembli | ENSMUST00000049519; ENSMUSP00000050446; ENSMUSG00000046879 [Q60766-1] ENSMUST00000097271; ENSMUSP00000094870; ENSMUSG00000046879 [Q60766-2] ENSMUST00000229243; ENSMUSP00000155118; ENSMUSG00000116444 [Q60766-1] ENSMUST00000230442; ENSMUSP00000154822; ENSMUSG00000116444 [Q60766-1] |
GeneIDi | 15944 |
KEGGi | mmu:15944 |
UCSCi | uc007ipg.1, mouse [Q60766-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U19119 mRNA Translation: AAB48942.1 AK002545 mRNA Translation: BAB22176.1 AK167558 mRNA Translation: BAE39622.1 AK171743 mRNA Translation: BAE42645.1 AL645849 Genomic DNA No translation available. BC145957 mRNA Translation: AAI45958.1 |
CCDSi | CCDS24588.1 [Q60766-1] CCDS88149.1 [Q60766-2] |
RefSeqi | NP_032352.1, NM_008326.1 [Q60766-1] |
3D structure databases
SMRi | Q60766 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200520, 2 interactors |
IntActi | Q60766, 2 interactors |
MINTi | Q60766 |
STRINGi | 10090.ENSMUSP00000050446 |
PTM databases
iPTMneti | Q60766 |
PhosphoSitePlusi | Q60766 |
SwissPalmi | Q60766 |
Proteomic databases
jPOSTi | Q60766 |
PaxDbi | Q60766 |
PeptideAtlasi | Q60766 |
PRIDEi | Q60766 |
ProteomicsDBi | 268995 [Q60766-1] 268996 [Q60766-2] |
Genome annotation databases
Ensembli | ENSMUST00000049519; ENSMUSP00000050446; ENSMUSG00000046879 [Q60766-1] ENSMUST00000097271; ENSMUSP00000094870; ENSMUSG00000046879 [Q60766-2] ENSMUST00000229243; ENSMUSP00000155118; ENSMUSG00000116444 [Q60766-1] ENSMUST00000230442; ENSMUSP00000154822; ENSMUSG00000116444 [Q60766-1] |
GeneIDi | 15944 |
KEGGi | mmu:15944 |
UCSCi | uc007ipg.1, mouse [Q60766-1] |
Organism-specific databases
CTDi | 15944 |
MGIi | MGI:107567, Irgm1 |
Phylogenomic databases
eggNOGi | ENOG502QS9R, Eukaryota |
GeneTreei | ENSGT00950000183007 |
InParanoidi | Q60766 |
OrthoDBi | 688334at2759 |
PhylomeDBi | Q60766 |
TreeFami | TF331897 |
Miscellaneous databases
BioGRID-ORCSi | 15944, 1 hit in 54 CRISPR screens |
ChiTaRSi | Irgm1, mouse |
PROi | PR:Q60766 |
RNActi | Q60766, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000046879, Expressed in submandibular gland and 241 other tissues |
ExpressionAtlasi | Q60766, baseline and differential |
Genevisiblei | Q60766, MM |
Family and domain databases
InterProi | View protein in InterPro IPR030385, G_IRG_dom IPR007743, Immunity-related_GTPase-like IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF05049, IIGP, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51716, G_IRG, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IRGM1_MOUSE | |
Accessioni | Q60766Primary (citable) accession number: Q60766 Secondary accession number(s): Q3TJ73 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | November 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families