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Protein

Probable E3 ubiquitin-protein ligase makorin-3

Gene

Mkrn3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.By similarity

Miscellaneous

Imprinted, expressed from the paternal chromosome only. The maternal methylation is established promptly after fertilization prior to syngamy.

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri92 – 119C3H1-type 1PROSITE-ProRule annotationAdd BLAST28
Zinc fingeri274 – 301C3H1-type 2PROSITE-ProRule annotationAdd BLAST28
Zinc fingeri347 – 401RING-typePROSITE-ProRule annotationAdd BLAST55
Zinc fingeri430 – 459C3H1-type 3PROSITE-ProRule annotationAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Probable E3 ubiquitin-protein ligase makorin-3 (EC:2.3.2.27)
Alternative name(s):
RING-type E3 ubiquitin transferase makorin-3Curated
Zinc finger protein 127
Gene namesi
Name:Mkrn3
Synonyms:Zfp127, Znf127
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2181178 Mkrn3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559601 – 544Probable E3 ubiquitin-protein ligase makorin-3Add BLAST544

Proteomic databases

MaxQBiQ60764
PaxDbiQ60764
PRIDEiQ60764

PTM databases

iPTMnetiQ60764
PhosphoSitePlusiQ60764

Expressioni

Tissue specificityi

Expressed in testis, brain, heart and kidney. Ubiquitously detected at low levels throughout the entire embryo, but expression is highest in the ventricular layers of the brain.2 Publications

Developmental stagei

Expressed at the blastocyst stage and the embryonic days 8-17, as well as in undifferentiated and differentiated embryonic stem cells. Expressed in the arcuate nucleus of both male and female animals. Levels of expression are highest on postnatal days 10 and 12, begin to decline on day 15, and reaches a nadir by days 18 to 22, at which time expression is 10 to 20% of the levels detected at 10 days. The timing of the decline in protein expression correlated with the ages at which arcuate KISS1 and TAC2 have been shown to increase, heralding the onset of puberty.2 Publications

Gene expression databases

BgeeiENSMUSG00000070527 Expressed in 125 organ(s), highest expression level in brain
CleanExiMM_MKRN3
GenevisibleiQ60764 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000091898

Structurei

3D structure databases

ProteinModelPortaliQ60764
SMRiQ60764
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni302 – 329Makorin-type Cys-HisAdd BLAST28

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri92 – 119C3H1-type 1PROSITE-ProRule annotationAdd BLAST28
Zinc fingeri274 – 301C3H1-type 2PROSITE-ProRule annotationAdd BLAST28
Zinc fingeri347 – 401RING-typePROSITE-ProRule annotationAdd BLAST55
Zinc fingeri430 – 459C3H1-type 3PROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1039 Eukaryota
ENOG410XRM0 LUCA
GeneTreeiENSGT00390000014093
HOGENOMiHOG000252976
HOVERGENiHBG066965
InParanoidiQ60764
KOiK15687
OMAiSEDQWDL
OrthoDBiEOG091G07DG
TreeFamiTF315108

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR026294 Makorin_3
IPR031644 MKRN1_C
IPR018957 Znf_C3HC4_RING-type
IPR000571 Znf_CCCH
IPR036855 Znf_CCCH_sf
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR11224:SF38 PTHR11224:SF38, 2 hits
PfamiView protein in Pfam
PF15815 MKRN1_C, 1 hit
PF00097 zf-C3HC4, 1 hit
PF00642 zf-CCCH, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00356 ZnF_C3H1, 3 hits
SUPFAMiSSF90229 SSF90229, 2 hits
PROSITEiView protein in PROSITE
PS50103 ZF_C3H1, 3 hits
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q60764-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEESTAPIEA HAAAGAEAGA EGGEGVSVPP PPQFEAAGAS AGVSSAPLQQ
60 70 80 90 100
ASGLAPLLVT PGPAIRRAAS LRPAPAEGGG ARSGPERNSG SWTKQILCRY
110 120 130 140 150
YLHGQCKEGD NCRYSHDLSG RRRSRGGQDA QPRASADRGP KMATRWEPPT
160 170 180 190 200
QEVAEAPPAA SSSSLPLIGS AAERGFTEAE IDNAGIRSAA ERGFSEAEID
210 220 230 240 250
NASLAAGAAA GAGAEGWEGA IEFVPGQPYR GRMVPPHGPE APLQSPAIER
260 270 280 290 300
EHMAMGMGMP MPVPMPMPVP MPVPMPLPLC RYAARGQCLR GDRCAYPHGE
310 320 330 340 350
ICDMCGQQAL HPWDAAQQEA HRRACVEAHE RDMELSFAVQ RSMDKVCGIC
360 370 380 390 400
MEVVYEKADP SDRRFGILFS CNHTYCLRCI RRWRSATQFE NRISKSCPQC
410 420 430 440 450
RVSSGFVIPS EFWVEEEEEK EKLVQQYKEG MSQKACRYFA GGLGHCPFGE
460 470 480 490 500
FCFYKHEYPE GWRDQPPRPD GGGSSSAYWH QVLEPVQLRE GNVLFKSRKK
510 520 530 540
EHSVLRLANQ LLKKLLCLRG SSSFSDDRWL LLQYQLEEYF SLNL
Length:544
Mass (Da):59,434
Last modified:July 27, 2011 - v2
Checksum:iAD9DF187A253D69B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti154A → V in AAA76863 (PubMed:10196368).Curated1
Sequence conflicti233M → V in AAA76863 (PubMed:10196368).Curated1
Sequence conflicti361S → T in AAA76863 (PubMed:10196368).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19106 Genomic DNA Translation: AAA76863.1
AK134870 mRNA Translation: BAE22320.1
BC054771 mRNA Translation: AAH54771.1
CCDSiCCDS21327.1
RefSeqiNP_035876.2, NM_011746.2
UniGeneiMm.146

Genome annotation databases

EnsembliENSMUST00000094340; ENSMUSP00000091898; ENSMUSG00000070527
GeneIDi22652
KEGGimmu:22652
UCSCiuc009hfi.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19106 Genomic DNA Translation: AAA76863.1
AK134870 mRNA Translation: BAE22320.1
BC054771 mRNA Translation: AAH54771.1
CCDSiCCDS21327.1
RefSeqiNP_035876.2, NM_011746.2
UniGeneiMm.146

3D structure databases

ProteinModelPortaliQ60764
SMRiQ60764
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000091898

PTM databases

iPTMnetiQ60764
PhosphoSitePlusiQ60764

Proteomic databases

MaxQBiQ60764
PaxDbiQ60764
PRIDEiQ60764

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000094340; ENSMUSP00000091898; ENSMUSG00000070527
GeneIDi22652
KEGGimmu:22652
UCSCiuc009hfi.1 mouse

Organism-specific databases

CTDi7681
MGIiMGI:2181178 Mkrn3

Phylogenomic databases

eggNOGiKOG1039 Eukaryota
ENOG410XRM0 LUCA
GeneTreeiENSGT00390000014093
HOGENOMiHOG000252976
HOVERGENiHBG066965
InParanoidiQ60764
KOiK15687
OMAiSEDQWDL
OrthoDBiEOG091G07DG
TreeFamiTF315108

Enzyme and pathway databases

UniPathwayi
UPA00143

Miscellaneous databases

PROiPR:Q60764
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000070527 Expressed in 125 organ(s), highest expression level in brain
CleanExiMM_MKRN3
GenevisibleiQ60764 MM

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR026294 Makorin_3
IPR031644 MKRN1_C
IPR018957 Znf_C3HC4_RING-type
IPR000571 Znf_CCCH
IPR036855 Znf_CCCH_sf
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR11224:SF38 PTHR11224:SF38, 2 hits
PfamiView protein in Pfam
PF15815 MKRN1_C, 1 hit
PF00097 zf-C3HC4, 1 hit
PF00642 zf-CCCH, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00356 ZnF_C3H1, 3 hits
SUPFAMiSSF90229 SSF90229, 2 hits
PROSITEiView protein in PROSITE
PS50103 ZF_C3H1, 3 hits
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMKRN3_MOUSE
AccessioniPrimary (citable) accession number: Q60764
Secondary accession number(s): Q3UY92, Q7TQE4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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