UniProtKB - Q60756 (TCF15_MOUSE)
Transcription factor 15
Tcf15
Functioni
Early transcription factor that plays a key role in somitogenesis, paraxial mesoderm development and regulation of stem cell pluripotency (PubMed:7597044, PubMed:8955271, PubMed:23395635, PubMed:32669716, PubMed:24038871).
Essential for the mesenchymal to epithelial transition associated with somite formation (PubMed:7597044, PubMed:8955271, PubMed:24038871).
Required for somite morphogenesis, thereby regulating patterning of the axial skeleton and skeletal muscles (PubMed:8955271).
Required for proper localization of somite epithelium markers during the mesenchymal to epithelial transition (PubMed:24038871).
Also plays a key role in regulation of stem cell pluripotency (PubMed:23395635, PubMed:32669716).
Promotes pluripotency exit of embryonic stem cells (ESCs) by priming ESCs for differentiation (PubMed:23395635).
Acts as a key regulator of self-renewal of hematopoietic stem cells (HSCs) by mediating HSCs quiescence and long-term self-renewal (PubMed:32669716).
Together with MEOX2, regulates transcription in heart endothelial cells to regulate fatty acid transport across heart endothelial cells (PubMed:25561514).
Acts by forming a heterodimer with another helix-loop-helix (bHLH) protein, such as TCF3/E12, that binds DNA on E-box motifs (5'-CANNTG-3') and activates transcription of target genes (PubMed:15226298, PubMed:23395635).
7 PublicationsGO - Molecular functioni
- bHLH transcription factor binding Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: UniProtKB
- E-box binding Source: UniProtKB
- protein dimerization activity Source: InterPro
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- anterior/posterior pattern specification Source: MGI
- developmental process Source: GO_Central
- ear development Source: MGI
- eating behavior Source: MGI
- establishment of epithelial cell apical/basal polarity Source: MGI
- mesenchymal to epithelial transition Source: MGI
- muscle organ development Source: MGI
- muscle organ morphogenesis Source: MGI
- negative regulation of hematopoietic stem cell differentiation Source: UniProtKB
- neuromuscular process controlling posture Source: MGI
- paraxial mesoderm development Source: MGI
- positive regulation of stem cell differentiation Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- post-anal tail morphogenesis Source: MGI
- regulation of extracellular matrix organization Source: MGI
- regulation of gene expression Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- respiratory system process Source: MGI
- skeletal system morphogenesis Source: MGI
- skin development Source: MGI
- somitogenesis Source: MGI
- stem cell population maintenance Source: UniProtKB
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Differentiation, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor 151 PublicationShort name: TCF-151 Publication Alternative name(s): Meso11 Publication Paraxis1 Publication Protein bHLH-EC21 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104664, Tcf15 |
VEuPathDBi | HostDB:ENSMUSG00000068079 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Nucleus
- nucleus Source: UniProtKB
- RNA polymerase II transcription regulator complex Source: NTNU_SB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 70 – 71 | RQ → KK: Does not affect formation of a heterodimer with TCF3 and ability to bind DNA. 1 Publication | 2 | |
Mutagenesisi | 75 | A → N: Does not affect formation of a heterodimer with TCF3 and ability to bind DNA. 1 Publication | 1 | |
Mutagenesisi | 90 | F → P: Impaired formation of a heterodimer with TCF3 and decreased ability to bind DNA. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127462 | 1 – 195 | Transcription factor 15Add BLAST | 195 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 60 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q60756 |
PaxDbi | Q60756 |
PRIDEi | Q60756 |
ProteomicsDBi | 263145 |
PTM databases
iPTMneti | Q60756 |
PhosphoSitePlusi | Q60756 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000068079, Expressed in heart and 191 other tissues |
Genevisiblei | Q60756, MM |
Interactioni
Subunit structurei
GO - Molecular functioni
- bHLH transcription factor binding Source: UniProtKB
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 204001, 2 interactors |
STRINGi | 10090.ENSMUSP00000086511 |
Miscellaneous databases
RNActi | Q60756, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 70 – 122 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 44 – 65 | DisorderedSequence analysisAdd BLAST | 22 |
Phylogenomic databases
eggNOGi | KOG4029, Eukaryota |
GeneTreei | ENSGT00940000161320 |
HOGENOMi | CLU_115077_0_0_1 |
InParanoidi | Q60756 |
OMAi | HPFSYPA |
OrthoDBi | 1511100at2759 |
PhylomeDBi | Q60756 |
TreeFami | TF315153 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAFALLRPVG AHVLYPDVRL LSEDEENRSE SDASDQSFGC CEGLEAARRG
60 70 80 90 100
PGPGSGRRAS NGAGPVVVVR QRQAANARER DRTQSVNTAF TALRTLIPTE
110 120 130 140 150
PVDRKLSKIE TLRLASSYIA HLANVLLLGD AADDGQPCFR AAGGGKSAVP
160 170 180 190
AADGRQPRSI CTFCLSNQRK GGSRRDLGGS CLKVRGVAPL RGPRR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 130 – 131 | DA → ELT in AAA86825 (PubMed:7729571).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18658 mRNA Translation: AAA86825.1 BC027533 mRNA Translation: AAH27533.1 U20164 Genomic DNA Translation: AAB48215.1 |
CCDSi | CCDS16878.1 |
RefSeqi | NP_033354.2, NM_009328.2 |
Genome annotation databases
Ensembli | ENSMUST00000089112; ENSMUSP00000086511; ENSMUSG00000068079 |
GeneIDi | 21407 |
KEGGi | mmu:21407 |
UCSCi | uc008nex.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18658 mRNA Translation: AAA86825.1 BC027533 mRNA Translation: AAH27533.1 U20164 Genomic DNA Translation: AAB48215.1 |
CCDSi | CCDS16878.1 |
RefSeqi | NP_033354.2, NM_009328.2 |
3D structure databases
AlphaFoldDBi | Q60756 |
SMRi | Q60756 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204001, 2 interactors |
STRINGi | 10090.ENSMUSP00000086511 |
PTM databases
iPTMneti | Q60756 |
PhosphoSitePlusi | Q60756 |
Proteomic databases
jPOSTi | Q60756 |
PaxDbi | Q60756 |
PRIDEi | Q60756 |
ProteomicsDBi | 263145 |
Protocols and materials databases
Antibodypediai | 6289, 108 antibodies from 21 providers |
DNASUi | 21407 |
Genome annotation databases
Ensembli | ENSMUST00000089112; ENSMUSP00000086511; ENSMUSG00000068079 |
GeneIDi | 21407 |
KEGGi | mmu:21407 |
UCSCi | uc008nex.1, mouse |
Organism-specific databases
CTDi | 6939 |
MGIi | MGI:104664, Tcf15 |
VEuPathDBi | HostDB:ENSMUSG00000068079 |
Phylogenomic databases
eggNOGi | KOG4029, Eukaryota |
GeneTreei | ENSGT00940000161320 |
HOGENOMi | CLU_115077_0_0_1 |
InParanoidi | Q60756 |
OMAi | HPFSYPA |
OrthoDBi | 1511100at2759 |
PhylomeDBi | Q60756 |
TreeFami | TF315153 |
Miscellaneous databases
BioGRID-ORCSi | 21407, 2 hits in 73 CRISPR screens |
ChiTaRSi | Tcf15, mouse |
PROi | PR:Q60756 |
RNActi | Q60756, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000068079, Expressed in heart and 191 other tissues |
Genevisiblei | Q60756, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TCF15_MOUSE | |
Accessioni | Q60756Primary (citable) accession number: Q60756 Secondary accession number(s): Q60788 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 25, 2002 |
Last sequence update: | November 25, 2002 | |
Last modified: | May 25, 2022 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot