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UniProtKB - Q60722 (ITF2_MOUSE)

Protein

Transcription factor 4

Gene

Tcf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Isoform 2 inhibits MYOD1 activation of the cardiac alpha-actin promoter. Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. May have a regulatory function in developmental processes as well as during neuronal plasticity.1 Publication

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-375170 CDO in myogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor 4
Short name:
TCF-4
Alternative name(s):
Class A helix-loop-helix transcription factor ME2
Immunoglobulin transcription factor 2
Short name:
ITF-2
Short name:
MITF-2
SL3-3 enhancer factor 2
Short name:
SEF-2
Gene namesi
Name:Tcf4
Synonyms:Itf2, Sef2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:98506 Tcf4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001272571 – 670Transcription factor 4Add BLAST670

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei66PhosphoserineBy similarity1
Modified residuei87PhosphoserineBy similarity1
Modified residuei92PhosphoserineBy similarity1
Modified residuei371PhosphoserineBy similarity1
Modified residuei514PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ60722
PeptideAtlasiQ60722
PRIDEiQ60722

PTM databases

iPTMnetiQ60722
PhosphoSitePlusiQ60722

Expressioni

Tissue specificityi

Expressed in the cerebral cortex, Purkinje and granule cell layers of the cerebellum, olfactory neuroepithelium, pyramidal cells of hippocampal layers CA1-CA4, and in the granular cells of the dentate gyrus.

Developmental stagei

Expressed in proliferative zones during development and in the adult in areas of neuronal plasticity. At embryonic day 12 (E12), expression is localized in the cortex, cerebellum, pons, medulla and spinal cord. From E18 to adulthood, high levels of expression are found in the pyramidal cells of hippocampal layers CA1-CA4, and in the granular cells of the dentate gyrus. At postnatal day 7, expression is high in the visual cortex and in the subependymal region extending from the anterior lateral ventricle into the olfactory bulb.

Gene expression databases

BgeeiENSMUSG00000053477 Expressed in 327 organ(s), highest expression level in rostral migratory stream
CleanExiMM_TCF4
ExpressionAtlasiQ60722 baseline and differential
GenevisibleiQ60722 MM

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Isoform 2 seems to form inactive heterodimers with MYOD1. Interacts with HIVEP2. Interacts with NEUROD2. Interacts with AGBL1 (By similarity). Interacts with BHLHA9.By similarity2 Publications

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi204006, 16 interactors
DIPiDIP-42841N
IntActiQ60722, 29 interactors
MINTiQ60722
STRINGi10090.ENSMUSP00000077577

Structurei

3D structure databases

ProteinModelPortaliQ60722
SMRiQ60722
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini567 – 620bHLHPROSITE-ProRule annotationAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 83Essential for MYOD1 inhibitionAdd BLAST83
Regioni378 – 399Leucine-zipperAdd BLAST22
Regioni622 – 645Class A specific domainAdd BLAST24

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi227 – 230Poly-Ser4

Phylogenomic databases

eggNOGiKOG3910 Eukaryota
ENOG410XYUA LUCA
GeneTreeiENSGT00510000046438
HOGENOMiHOG000234180
HOVERGENiHBG003854
InParanoidiQ60722
KOiK15603
PhylomeDBiQ60722
TreeFamiTF321672

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 37 potential isoforms that are computationally mapped.Show allAlign All

Isoform 2 (identifier: Q60722-1) [UniParc]FASTAAdd to basket
Also known as: MITF-2B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MHHQQRMAAL GTDKELSDLL DFSAMFSPPV SSGKNGPTSL ASGHFTGSNV 
        60         70         80         90        100
EDRSSSGSWG TGGHPSPSRN YGDGTPYDHM TSRDLGSHDN LSPPFVNSRI 
       110        120        130        140        150
QSKTERGSYS SYGRENVQGC HQQSLLGGDM DMGNPGTLSP TKPGSQYYQY 
       160        170        180        190        200
SSNNARRRPL HSSAMEVQTK KVRKVPPGLP SSVYAPSAST ADYNRDSPGY 
       210        220        230        240        250
PSSKPAASTF PSSFFMQDGH HSSDPWSSSS GMNQPGYGGM LGNSSHIPQS 
       260        270        280        290        300
SSYCSLHPHE RLSYPSHSSA DINSSLPPMS TFHRSGTNHY STSSCTPPAN 
       310        320        330        340        350
GTDSIMANRG TGAAGSSQTG DALGKALASI YSPDHTNNSF SSNPSTPVGS 
       360        370        380        390        400
PPSLSAGTAV WSRNGGQASS SPNYEGPLHS LQSRIEDRLE RLDDAIHVLR 
       410        420        430        440        450
NHAVGPSTAV PGGHGDMHGI MGPSHNGAMG SLGSGYGTSL LSANRHSLMV 
       460        470        480        490        500
GAHREDGVAL RGSHSLLPNQ VPVPQLPVQS ATSPDLNPPQ DPYRGMPPGL 
       510        520        530        540        550
QGQSVSSGSS EIKSDDEGDE NLQDTKSSED KKLDDDKKDI KSITRSRSSN 
       560        570        580        590        600
NDDEDLTPEQ KAEREKERRM ANNARERLRV RDINEAFKEL GRMVQLHLKS 
       610        620        630        640        650
DKPQTKLLIL HQAVAVILSL EQQVRERNLN PKAACLKRRE EEKVSSEPPP 
       660        670
LSLAGPHPGM GDAANHMGQM
Length:670
Mass (Da):71,625
Last modified:November 1, 1996 - v1
Checksum:iF21CF79FDC0BF09E
GO
Isoform 1 (identifier: Q60722-2) [UniParc]FASTAAdd to basket
Also known as: MITF-2A

The sequence of this isoform differs from the canonical sequence as follows:
     1-159: Missing.
     160-182: LHSSAMEVQTKKVRKVPPGLPSS → MYCAYTIPGMGGNSLMYYYNGKA

Show »
Length:511
Mass (Da):54,591
Checksum:i44BA079B0A6CCE48
GO
Isoform 3 (identifier: Q60722-3) [UniParc]FASTAAdd to basket
Also known as: MITF-2B-delta

The sequence of this isoform differs from the canonical sequence as follows:
     545-548: Missing.

Show »
Length:666
Mass (Da):71,138
Checksum:iE735F6DD18A1E37F
GO

Computationally mapped potential isoform sequencesi

There are 37 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q91YV0Q91YV0_MOUSE
Tcf4 protein
Tcf4 mCG_9251
507Annotation score:
E9Q8G4E9Q8G4_MOUSE
Transcription factor 4
Tcf4
692Annotation score:
A0A0J9YTR9A0A0J9YTR9_MOUSE
Transcription factor 4
Tcf4
507Annotation score:
A0A0J9YUG4A0A0J9YUG4_MOUSE
Transcription factor 4
Tcf4 mCG_9251
537Annotation score:
A0A0J9YUZ0A0A0J9YUZ0_MOUSE
Transcription factor 4
Tcf4
646Annotation score:
A0A0J9YTU7A0A0J9YTU7_MOUSE
Transcription factor 4
Tcf4
541Annotation score:
A0A0J9YUP9A0A0J9YUP9_MOUSE
Transcription factor 4
Tcf4 mCG_9251
642Annotation score:
A0A0J9YTW1A0A0J9YTW1_MOUSE
Transcription factor 4
Tcf4
587Annotation score:
A0A0J9YVI1A0A0J9YVI1_MOUSE
Transcription factor 4
Tcf4
610Annotation score:
A0A0J9YVG2A0A0J9YVG2_MOUSE
Transcription factor 4
Tcf4
641Annotation score:
There are more potential isoformsShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti159P → S in CAA62868 (PubMed:8978694).Curated1
Sequence conflicti180P → A in CAA62868 (PubMed:8978694).Curated1
Sequence conflicti238G → A in AAB32662 (PubMed:7984047).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0021131 – 159Missing in isoform 1. 1 PublicationAdd BLAST159
Alternative sequenceiVSP_002114160 – 182LHSSA…GLPSS → MYCAYTIPGMGGNSLMYYYN GKA in isoform 1. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_002115545 – 548Missing in isoform 3. 2 Publications4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S75870 mRNA Translation: AAB32662.1
U16321 mRNA Translation: AAC52414.1
U16322 mRNA Translation: AAC52415.1
X91753 mRNA Translation: CAA62868.1
AK081012 mRNA Translation: BAC38116.1
BC043050 mRNA Translation: AAH43050.1
CCDSiCCDS29329.1 [Q60722-1]
CCDS50315.1 [Q60722-3]
PIRiI52648
RefSeqiNP_001077436.1, NM_001083967.1 [Q60722-3]
NP_038713.1, NM_013685.2 [Q60722-1]
XP_006525809.1, XM_006525746.3 [Q60722-1]
XP_006525810.1, XM_006525747.3
XP_006525813.1, XM_006525750.3
XP_006525825.1, XM_006525762.3
XP_017173346.1, XM_017317857.1 [Q60722-3]
UniGeneiMm.4269

Genome annotation databases

EnsembliENSMUST00000078486; ENSMUSP00000077577; ENSMUSG00000053477 [Q60722-1]
ENSMUST00000114978; ENSMUSP00000110629; ENSMUSG00000053477 [Q60722-2]
ENSMUST00000114980; ENSMUSP00000110631; ENSMUSG00000053477 [Q60722-1]
ENSMUST00000114982; ENSMUSP00000110633; ENSMUSG00000053477 [Q60722-3]
ENSMUST00000114985; ENSMUSP00000110636; ENSMUSG00000053477 [Q60722-1]
ENSMUST00000202116; ENSMUSP00000144512; ENSMUSG00000053477 [Q60722-3]
GeneIDi21413
KEGGimmu:21413
UCSCiuc008fnq.1 mouse [Q60722-1]
uc008fns.1 mouse [Q60722-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S75870 mRNA Translation: AAB32662.1
U16321 mRNA Translation: AAC52414.1
U16322 mRNA Translation: AAC52415.1
X91753 mRNA Translation: CAA62868.1
AK081012 mRNA Translation: BAC38116.1
BC043050 mRNA Translation: AAH43050.1
CCDSiCCDS29329.1 [Q60722-1]
CCDS50315.1 [Q60722-3]
PIRiI52648
RefSeqiNP_001077436.1, NM_001083967.1 [Q60722-3]
NP_038713.1, NM_013685.2 [Q60722-1]
XP_006525809.1, XM_006525746.3 [Q60722-1]
XP_006525810.1, XM_006525747.3
XP_006525813.1, XM_006525750.3
XP_006525825.1, XM_006525762.3
XP_017173346.1, XM_017317857.1 [Q60722-3]
UniGeneiMm.4269

3D structure databases

ProteinModelPortaliQ60722
SMRiQ60722
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204006, 16 interactors
DIPiDIP-42841N
IntActiQ60722, 29 interactors
MINTiQ60722
STRINGi10090.ENSMUSP00000077577

PTM databases

iPTMnetiQ60722
PhosphoSitePlusiQ60722

Proteomic databases

PaxDbiQ60722
PeptideAtlasiQ60722
PRIDEiQ60722

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078486; ENSMUSP00000077577; ENSMUSG00000053477 [Q60722-1]
ENSMUST00000114978; ENSMUSP00000110629; ENSMUSG00000053477 [Q60722-2]
ENSMUST00000114980; ENSMUSP00000110631; ENSMUSG00000053477 [Q60722-1]
ENSMUST00000114982; ENSMUSP00000110633; ENSMUSG00000053477 [Q60722-3]
ENSMUST00000114985; ENSMUSP00000110636; ENSMUSG00000053477 [Q60722-1]
ENSMUST00000202116; ENSMUSP00000144512; ENSMUSG00000053477 [Q60722-3]
GeneIDi21413
KEGGimmu:21413
UCSCiuc008fnq.1 mouse [Q60722-1]
uc008fns.1 mouse [Q60722-3]

Organism-specific databases

CTDi6925
MGIiMGI:98506 Tcf4

Phylogenomic databases

eggNOGiKOG3910 Eukaryota
ENOG410XYUA LUCA
GeneTreeiENSGT00510000046438
HOGENOMiHOG000234180
HOVERGENiHBG003854
InParanoidiQ60722
KOiK15603
PhylomeDBiQ60722
TreeFamiTF321672

Enzyme and pathway databases

ReactomeiR-MMU-375170 CDO in myogenesis

Miscellaneous databases

ChiTaRSiTcf4 mouse
PROiPR:Q60722
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000053477 Expressed in 327 organ(s), highest expression level in rostral migratory stream
CleanExiMM_TCF4
ExpressionAtlasiQ60722 baseline and differential
GenevisibleiQ60722 MM

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiITF2_MOUSE
AccessioniPrimary (citable) accession number: Q60722
Secondary accession number(s): Q60721
, Q62211, Q64072, Q80UE8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: September 12, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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