UniProtKB - Q60722 (ITF2_MOUSE)
Transcription factor 4
Tcf4
Functioni
Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Isoform 2 inhibits MYOD1 activation of the cardiac alpha-actin promoter. Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. May have a regulatory function in developmental processes as well as during neuronal plasticity.
1 PublicationGO - Molecular functioni
- beta-catenin binding Source: MGI
- bHLH transcription factor binding Source: MGI
- chromatin binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: BHF-UCL
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: BHF-UCL
- double-stranded DNA binding Source: MGI
- E-box binding Source: UniProtKB
- identical protein binding Source: MGI
- protein C-terminus binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- protein self-association Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: BHF-UCL
- sequence-specific double-stranded DNA binding Source: MGI
- TFIIB-class transcription factor binding Source: BHF-UCL
- transcription corepressor activity Source: MGI
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- endothelial cell activation Source: MGI
- negative regulation of angiogenesis Source: MGI
- negative regulation of gene expression Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- nervous system development Source: UniProtKB-KW
- positive regulation of neuron differentiation Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- protein-DNA complex assembly Source: BHF-UCL
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- regulation of vascular endothelial growth factor signaling pathway Source: MGI
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Differentiation, Neurogenesis, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-525793, Myogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor 4Short name: TCF-4 Alternative name(s): Class A helix-loop-helix transcription factor ME2 Immunoglobulin transcription factor 2 Short name: ITF-2 Short name: MITF-2 SL3-3 enhancer factor 2 Short name: SEF-2 |
Gene namesi | Name:Tcf4 Synonyms:Itf2, Sef2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98506, Tcf4 |
VEuPathDBi | HostDB:ENSMUSG00000053477 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- beta-catenin-TCF complex Source: MGI
- beta-catenin-TCF7L2 complex Source: MGI
- nucleus Source: MGI
Other locations
- chromatin Source: MGI
- transcription regulator complex Source: BHF-UCL
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127257 | 1 – 670 | Transcription factor 4Add BLAST | 670 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 66 | PhosphoserineBy similarity | 1 | |
Modified residuei | 87 | PhosphoserineBy similarity | 1 | |
Modified residuei | 92 | PhosphoserineBy similarity | 1 | |
Modified residuei | 371 | PhosphoserineBy similarity | 1 | |
Modified residuei | 514 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q60722 |
MaxQBi | Q60722 |
PaxDbi | Q60722 |
PeptideAtlasi | Q60722 |
PRIDEi | Q60722 |
ProteomicsDBi | 268903 [Q60722-1] 268904 [Q60722-2] 268905 [Q60722-3] |
PTM databases
iPTMneti | Q60722 |
PhosphoSitePlusi | Q60722 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000053477, Expressed in rostral migratory stream and 344 other tissues |
ExpressionAtlasi | Q60722, baseline and differential |
Genevisiblei | Q60722, MM |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Isoform 2 seems to form inactive heterodimers with MYOD1.
Interacts with HIVEP2.
Interacts with NEUROD2.
Interacts with AGBL1 (By similarity).
Interacts with BHLHA9.
By similarity2 PublicationsBinary interactionsi
Q60722
With | #Exp. | IntAct |
---|---|---|
Cbfa2t3 [O54972] | 2 | EBI-310070,EBI-8006703 |
Ldb1 [P70662] | 2 | EBI-310070,EBI-6272082 |
Mllt10 [O54826] | 3 | EBI-310070,EBI-8459555 |
GO - Molecular functioni
- beta-catenin binding Source: MGI
- bHLH transcription factor binding Source: MGI
- identical protein binding Source: MGI
- protein C-terminus binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- protein self-association Source: MGI
- TFIIB-class transcription factor binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 204006, 16 interactors |
DIPi | DIP-42841N |
IntActi | Q60722, 31 interactors |
MINTi | Q60722 |
STRINGi | 10090.ENSMUSP00000110636 |
Miscellaneous databases
RNActi | Q60722, protein |
Structurei
3D structure databases
BMRBi | Q60722 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 567 – 620 | bHLHPROSITE-ProRule annotationAdd BLAST | 54 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 83 | Essential for MYOD1 inhibitionAdd BLAST | 83 | |
Regioni | 24 – 244 | DisorderedSequence analysisAdd BLAST | 221 | |
Regioni | 262 – 320 | DisorderedSequence analysisAdd BLAST | 59 | |
Regioni | 335 – 378 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 378 – 399 | Leucine-zipperAdd BLAST | 22 | |
Regioni | 406 – 426 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 465 – 573 | DisorderedSequence analysisAdd BLAST | 109 | |
Regioni | 622 – 645 | Class A specific domainAdd BLAST | 24 | |
Regioni | 637 – 670 | DisorderedSequence analysisAdd BLAST | 34 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 28 – 65 | Polar residuesSequence analysisAdd BLAST | 38 | |
Compositional biasi | 86 – 123 | Polar residuesSequence analysisAdd BLAST | 38 | |
Compositional biasi | 138 – 159 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 183 – 244 | Polar residuesSequence analysisAdd BLAST | 62 | |
Compositional biasi | 266 – 318 | Polar residuesSequence analysisAdd BLAST | 53 | |
Compositional biasi | 468 – 487 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 512 – 573 | Basic and acidic residuesSequence analysisAdd BLAST | 62 |
Phylogenomic databases
eggNOGi | KOG3910, Eukaryota |
GeneTreei | ENSGT00940000159129 |
InParanoidi | Q60722 |
OrthoDBi | 571132at2759 |
PhylomeDBi | Q60722 |
TreeFami | TF321672 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 37 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MHHQQRMAAL GTDKELSDLL DFSAMFSPPV SSGKNGPTSL ASGHFTGSNV
60 70 80 90 100
EDRSSSGSWG TGGHPSPSRN YGDGTPYDHM TSRDLGSHDN LSPPFVNSRI
110 120 130 140 150
QSKTERGSYS SYGRENVQGC HQQSLLGGDM DMGNPGTLSP TKPGSQYYQY
160 170 180 190 200
SSNNARRRPL HSSAMEVQTK KVRKVPPGLP SSVYAPSAST ADYNRDSPGY
210 220 230 240 250
PSSKPAASTF PSSFFMQDGH HSSDPWSSSS GMNQPGYGGM LGNSSHIPQS
260 270 280 290 300
SSYCSLHPHE RLSYPSHSSA DINSSLPPMS TFHRSGTNHY STSSCTPPAN
310 320 330 340 350
GTDSIMANRG TGAAGSSQTG DALGKALASI YSPDHTNNSF SSNPSTPVGS
360 370 380 390 400
PPSLSAGTAV WSRNGGQASS SPNYEGPLHS LQSRIEDRLE RLDDAIHVLR
410 420 430 440 450
NHAVGPSTAV PGGHGDMHGI MGPSHNGAMG SLGSGYGTSL LSANRHSLMV
460 470 480 490 500
GAHREDGVAL RGSHSLLPNQ VPVPQLPVQS ATSPDLNPPQ DPYRGMPPGL
510 520 530 540 550
QGQSVSSGSS EIKSDDEGDE NLQDTKSSED KKLDDDKKDI KSITRSRSSN
560 570 580 590 600
NDDEDLTPEQ KAEREKERRM ANNARERLRV RDINEAFKEL GRMVQLHLKS
610 620 630 640 650
DKPQTKLLIL HQAVAVILSL EQQVRERNLN PKAACLKRRE EEKVSSEPPP
660 670
LSLAGPHPGM GDAANHMGQM
Computationally mapped potential isoform sequencesi
There are 37 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q8G4 | E9Q8G4_MOUSE | Transcription factor 4 | Tcf4 | 692 | Annotation score: | ||
Q91YV0 | Q91YV0_MOUSE | Tcf4 protein | Tcf4 | 507 | Annotation score: | ||
A0A0J9YTU7 | A0A0J9YTU7_MOUSE | Transcription factor 4 | Tcf4 | 541 | Annotation score: | ||
A0A0J9YUG4 | A0A0J9YUG4_MOUSE | Transcription factor 4 | Tcf4 | 537 | Annotation score: | ||
A0A0J9YUZ0 | A0A0J9YUZ0_MOUSE | Transcription factor 4 | Tcf4 | 646 | Annotation score: | ||
A0A0J9YTR9 | A0A0J9YTR9_MOUSE | Transcription factor 4 | Tcf4 | 507 | Annotation score: | ||
A0A0J9YTW1 | A0A0J9YTW1_MOUSE | Transcription factor 4 | Tcf4 | 587 | Annotation score: | ||
A0A0J9YU37 | A0A0J9YU37_MOUSE | Transcription factor 4 | Tcf4 | 606 | Annotation score: | ||
A0A0J9YUK1 | A0A0J9YUK1_MOUSE | Transcription factor 4 | Tcf4 | 641 | Annotation score: | ||
A0A0J9YUP9 | A0A0J9YUP9_MOUSE | Transcription factor 4 | Tcf4 | 642 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 159 | P → S in CAA62868 (PubMed:8978694).Curated | 1 | |
Sequence conflicti | 180 | P → A in CAA62868 (PubMed:8978694).Curated | 1 | |
Sequence conflicti | 238 | G → A in AAB32662 (PubMed:7984047).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002113 | 1 – 159 | Missing in isoform 1. 1 PublicationAdd BLAST | 159 | |
Alternative sequenceiVSP_002114 | 160 – 182 | LHSSA…GLPSS → MYCAYTIPGMGGNSLMYYYN GKA in isoform 1. 1 PublicationAdd BLAST | 23 | |
Alternative sequenceiVSP_002115 | 545 – 548 | Missing in isoform 3. 2 Publications | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S75870 mRNA Translation: AAB32662.1 U16321 mRNA Translation: AAC52414.1 U16322 mRNA Translation: AAC52415.1 X91753 mRNA Translation: CAA62868.1 AK081012 mRNA Translation: BAC38116.1 BC043050 mRNA Translation: AAH43050.1 |
CCDSi | CCDS29329.1 [Q60722-1] CCDS50315.1 [Q60722-3] |
PIRi | I52648 |
RefSeqi | NP_001077436.1, NM_001083967.1 [Q60722-3] NP_038713.1, NM_013685.2 [Q60722-1] XP_006525809.1, XM_006525746.3 [Q60722-1] XP_006525810.1, XM_006525747.3 XP_006525813.1, XM_006525750.3 XP_006525825.1, XM_006525762.3 XP_017173346.1, XM_017317857.1 [Q60722-3] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S75870 mRNA Translation: AAB32662.1 U16321 mRNA Translation: AAC52414.1 U16322 mRNA Translation: AAC52415.1 X91753 mRNA Translation: CAA62868.1 AK081012 mRNA Translation: BAC38116.1 BC043050 mRNA Translation: AAH43050.1 |
CCDSi | CCDS29329.1 [Q60722-1] CCDS50315.1 [Q60722-3] |
PIRi | I52648 |
RefSeqi | NP_001077436.1, NM_001083967.1 [Q60722-3] NP_038713.1, NM_013685.2 [Q60722-1] XP_006525809.1, XM_006525746.3 [Q60722-1] XP_006525810.1, XM_006525747.3 XP_006525813.1, XM_006525750.3 XP_006525825.1, XM_006525762.3 XP_017173346.1, XM_017317857.1 [Q60722-3] |
3D structure databases
BMRBi | Q60722 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 204006, 16 interactors |
DIPi | DIP-42841N |
IntActi | Q60722, 31 interactors |
MINTi | Q60722 |
STRINGi | 10090.ENSMUSP00000110636 |
PTM databases
iPTMneti | Q60722 |
PhosphoSitePlusi | Q60722 |
Proteomic databases
jPOSTi | Q60722 |
MaxQBi | Q60722 |
PaxDbi | Q60722 |
PeptideAtlasi | Q60722 |
PRIDEi | Q60722 |
ProteomicsDBi | 268903 [Q60722-1] 268904 [Q60722-2] 268905 [Q60722-3] |
Protocols and materials databases
Antibodypediai | 9596, 419 antibodies from 36 providers |
DNASUi | 21413 |
Genome annotation databases
Organism-specific databases
CTDi | 6925 |
MGIi | MGI:98506, Tcf4 |
VEuPathDBi | HostDB:ENSMUSG00000053477 |
Phylogenomic databases
eggNOGi | KOG3910, Eukaryota |
GeneTreei | ENSGT00940000159129 |
InParanoidi | Q60722 |
OrthoDBi | 571132at2759 |
PhylomeDBi | Q60722 |
TreeFami | TF321672 |
Enzyme and pathway databases
Reactomei | R-MMU-525793, Myogenesis |
Miscellaneous databases
BioGRID-ORCSi | 21413, 0 hits in 64 CRISPR screens |
ChiTaRSi | Tcf4, mouse |
PROi | PR:Q60722 |
RNActi | Q60722, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000053477, Expressed in rostral migratory stream and 344 other tissues |
ExpressionAtlasi | Q60722, baseline and differential |
Genevisiblei | Q60722, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ITF2_MOUSE | |
Accessioni | Q60722Primary (citable) accession number: Q60722 Secondary accession number(s): Q60721 Q80UE8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 187 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot