Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ganglioside GM2 activator

Gene

Gm2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity.1 Publication

GO - Molecular functioni

  • beta-N-acetylgalactosaminidase activity Source: Ensembl
  • beta-N-acetylhexosaminidase activity Source: MGI
  • enzyme activator activity Source: MGI
  • lipid transporter activity Source: Ensembl
  • phospholipase activator activity Source: Ensembl

GO - Biological processi

  • ganglioside catabolic process Source: MGI
  • learning or memory Source: MGI
  • lipid storage Source: MGI
  • nervous system process Source: MGI
  • neuromuscular process controlling balance Source: MGI
  • oligosaccharide catabolic process Source: MGI
  • positive regulation of hydrolase activity Source: Ensembl

Keywordsi

Molecular functionHydrolase
Biological processLipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-1660662 Glycosphingolipid metabolism
R-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Ganglioside GM2 activator
Alternative name(s):
Cerebroside sulfate activator protein
GM2-AP
Sphingolipid activator protein 3
Short name:
SAP-3
Gene namesi
Name:Gm2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:95762 Gm2a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi168Y → S: Abolishes phospholipid binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000003164321 – 193Ganglioside GM2 activatorAdd BLAST173

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi39 ↔ 1831 Publication
Disulfide bondi99 ↔ 1061 Publication
Disulfide bondi112 ↔ 1381 Publication
Disulfide bondi125 ↔ 1361 Publication
Glycosylationi151N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ60648
MaxQBiQ60648
PaxDbiQ60648
PRIDEiQ60648

Expressioni

Tissue specificityi

Widely expressed. Most abundant in kidney and testis.

Gene expression databases

BgeeiENSMUSG00000000594
CleanExiMM_GM2A
ExpressionAtlasiQ60648 baseline and differential
GenevisibleiQ60648 MM

Interactioni

Protein-protein interaction databases

IntActiQ60648, 2 interactors
MINTiQ60648
STRINGi10090.ENSMUSP00000000608

Structurei

Secondary structure

1193
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi35 – 39Combined sources5
Helixi40 – 42Combined sources3
Beta strandi44 – 58Combined sources15
Beta strandi60 – 74Combined sources15
Beta strandi81 – 90Combined sources10
Beta strandi93 – 96Combined sources4
Beta strandi107 – 109Combined sources3
Helixi111 – 118Combined sources8
Turni127 – 133Combined sources7
Beta strandi142 – 153Combined sources12
Beta strandi165 – 176Combined sources12
Beta strandi179 – 190Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AGCX-ray2.50A32-193[»]
ProteinModelPortaliQ60648
SMRiQ60648
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ60648

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IX9N Eukaryota
ENOG4111JSM LUCA
GeneTreeiENSGT00390000003288
HOGENOMiHOG000031350
HOVERGENiHBG000260
InParanoidiQ60648
KOiK12383
OMAiWLSTGNY
OrthoDBiEOG091G0VEB
PhylomeDBiQ60648
TreeFamiTF353575

Family and domain databases

Gene3Di2.70.220.10, 1 hit
InterProiView protein in InterPro
IPR028996 GM2-AP
IPR036846 GM2-AP_sf
IPR003172 ML_dom
PANTHERiPTHR17357 PTHR17357, 1 hit
PfamiView protein in Pfam
PF02221 E1_DerP2_DerF2, 1 hit
SMARTiView protein in SMART
SM00737 ML, 1 hit
SUPFAMiSSF63707 SSF63707, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q60648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRLPLLLLL GLLLAGSVAP ARLVPKRLSQ LGGFSWDNCD EGKDPAVIKS
60 70 80 90 100
LTIQPDPIVV PGDVVVSLEG KTSVPLTAPQ KVELTVEKEV AGFWVKIPCV
110 120 130 140 150
EQLGSCSYEN ICDLIDEYIP PGESCPEPLH TYGLPCHCPF KEGTYSLPTS
160 170 180 190
NFTVPDLELP SWLSTGNYRI QSILSSGGKR LGCIKIAASL KGR
Length:193
Mass (Da):20,824
Last modified:November 1, 1997 - v2
Checksum:i59CC4ABE56FA1FC7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53I → T in AAA21543 (PubMed:7713516).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09816 mRNA Translation: AAA21543.1
L19526 mRNA Translation: AAA61929.1
U34359
, U34356, U34357, U34358 Genomic DNA Translation: AAB06275.1 Sequence problems.
BC004651 mRNA Translation: AAH04651.1
CCDSiCCDS24707.1
PIRiS35613
RefSeqiNP_034429.1, NM_010299.3
UniGeneiMm.287807

Genome annotation databases

EnsembliENSMUST00000000608; ENSMUSP00000000608; ENSMUSG00000000594
GeneIDi14667
KEGGimmu:14667
UCSCiuc007iyw.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSAP3_MOUSE
AccessioniPrimary (citable) accession number: Q60648
Secondary accession number(s): Q61610, Q61819
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health