UniProtKB - Q60632 (COT1_MOUSE)
COUP transcription factor 1
Nr2f1
Functioni
Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG (By similarity).
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 82 – 157 | Nuclear receptorPROSITE-ProRule annotationAdd BLAST | 76 | |
Zinc fingeri | 85 – 105 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 121 – 145 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- nuclear receptor activity Source: GO_Central
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- zinc ion binding Source: InterPro
GO - Biological processi
- anatomical structure development Source: GO_Central
- cell differentiation Source: GO_Central
- cerebral cortex regionalization Source: MGI
- forebrain development Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- neuron migration Source: MGI
- positive regulation of production of miRNAs involved in gene silencing by miRNA Source: MGI
- positive regulation of transcription by RNA polymerase II Source: GO_Central
- regulation of gene expression Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding, Receptor, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway |
Names & Taxonomyi
Protein namesi | Recommended name: COUP transcription factor 1Short name: COUP-TF1 Alternative name(s): COUP transcription factor I Short name: COUP-TF I Nuclear receptor subfamily 2 group F member 1 V-erbA-related protein 3 Short name: EAR-3 |
Gene namesi | Name:Nr2f1 Synonyms:Erbal3, Tfcoup1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1352451, Nr2f1 |
Subcellular locationi
Nucleus
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053603 | 1 – 422 | COUP transcription factor 1Add BLAST | 422 |
Proteomic databases
MaxQBi | Q60632 |
PaxDbi | Q60632 |
PeptideAtlasi | Q60632 |
PRIDEi | Q60632 |
ProteomicsDBi | 285253 |
PTM databases
iPTMneti | Q60632 |
PhosphoSitePlusi | Q60632 |
Expressioni
Developmental stagei
Interactioni
Subunit structurei
Binds DNA as dimer; homodimer and probable heterodimer with NR2F6.
Interacts with GTF2B; this interaction is direct.
Interacts with COPS2.
By similarityProtein-protein interaction databases
BioGRIDi | 199495, 1 interactor |
STRINGi | 10090.ENSMUSP00000089036 |
Miscellaneous databases
RNActi | Q60632, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 183 – 409 | NR LBDPROSITE-ProRule annotationAdd BLAST | 227 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 80 | DisorderedSequence analysisAdd BLAST | 80 | |
Regioni | 343 – 422 | Important for dimerizationAdd BLAST | 80 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 85 – 105 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 121 – 145 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3575, Eukaryota |
InParanoidi | Q60632 |
OrthoDBi | 666130at2759 |
PhylomeDBi | Q60632 |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAMVVSSWRD PQDDVAGGNP GGPNPAAQAA RGGGGGAGEQ QQAGSGAPHT
60 70 80 90 100
PQTPGQPGAP ATPGTQGDKG QGPPGSGQSQ QHIECVVCGD KSSGKHYGQF
110 120 130 140 150
TCEGCKSFFK RSVRRNLTYT CRANRNCPID QHHRNQCQYC RLKKCLKVGM
160 170 180 190 200
RREAVQRGRM PPTQPNPGQY ALTNGDPLNG HCYLSGYISL LLRAEPYPTS
210 220 230 240 250
RYGSQCMQPN NIMGIENICE LAARLLFSAV EWARNIPFFP DLQITDQVSL
260 270 280 290 300
LRLTWSELFV LNAAQCSMPL HVAPLLAAAG LHASPMSADR VVAFMDHIRI
310 320 330 340 350
FQEQVEKLKA LHVDSAEYSC LKAIVLFTSD ACGLSDAAHI ESLQEKSQCA
360 370 380 390 400
LEEYVRSQYP NQPSRFGKLL LRLPSLRTVS SSVIEQLFFV RLVGKTPIET
410 420
LIRDMLLSGS SFNWPYMSIQ CS
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ32NY6 | Q32NY6_MOUSE | COUP transcription factor 1 | Nr2f1 | 420 | Annotation score: | ||
F7CII1 | F7CII1_MOUSE | COUP transcription factor 1 | Nr2f1 | 273 | Annotation score: | ||
A0A286YD96 | A0A286YD96_MOUSE | COUP transcription factor 1 | Nr2f1 | 95 | Annotation score: | ||
B8JJI9 | B8JJI9_MOUSE | COUP transcription factor 1 | Nr2f1 | 263 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 36 – 37 | Missing in AAA19853 (PubMed:7947324).Curated | 2 | |
Sequence conflicti | 66 | Q → A in AAA19853 (PubMed:7947324).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U07625 mRNA Translation: AAA19853.1 X74134 mRNA Translation: CAA52231.1 |
PIRi | I48305 |
RefSeqi | NP_034281.2, NM_010151.3 |
Genome annotation databases
GeneIDi | 13865 |
KEGGi | mmu:13865 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U07625 mRNA Translation: AAA19853.1 X74134 mRNA Translation: CAA52231.1 |
PIRi | I48305 |
RefSeqi | NP_034281.2, NM_010151.3 |
3D structure databases
SMRi | Q60632 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199495, 1 interactor |
STRINGi | 10090.ENSMUSP00000089036 |
PTM databases
iPTMneti | Q60632 |
PhosphoSitePlusi | Q60632 |
Proteomic databases
MaxQBi | Q60632 |
PaxDbi | Q60632 |
PeptideAtlasi | Q60632 |
PRIDEi | Q60632 |
ProteomicsDBi | 285253 |
Protocols and materials databases
DNASUi | 13865 |
Genome annotation databases
GeneIDi | 13865 |
KEGGi | mmu:13865 |
Organism-specific databases
CTDi | 7025 |
MGIi | MGI:1352451, Nr2f1 |
Phylogenomic databases
eggNOGi | KOG3575, Eukaryota |
InParanoidi | Q60632 |
OrthoDBi | 666130at2759 |
PhylomeDBi | Q60632 |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway |
Miscellaneous databases
BioGRID-ORCSi | 13865, 0 hits in 62 CRISPR screens |
ChiTaRSi | Nr2f1, mouse |
PROi | PR:Q60632 |
RNActi | Q60632, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | COT1_MOUSE | |
Accessioni | Q60632Primary (citable) accession number: Q60632 Secondary accession number(s): Q61438 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | May 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 164 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families