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Protein

DNA-binding protein SATB1

Gene

Satb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis (By similarity). Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes.By similarity9 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei390Matrix attachment region (MAR) DNABy similarity1
Binding sitei425Matrix attachment region (MAR) DNABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi361 – 448CUT 1PROSITE-ProRule annotationAdd BLAST88
DNA bindingi484 – 571CUT 2PROSITE-ProRule annotationAdd BLAST88
DNA bindingi646 – 705HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-111465 Apoptotic cleavage of cellular proteins
R-MMU-4551638 SUMOylation of chromatin organization proteins

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein SATB1
Alternative name(s):
Special AT-rich sequence-binding protein 1
Gene namesi
Name:Satb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:105084 Satb1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are small in size, have disproportionately small thymi and spleens, and die at 3 weeks of age. Multiple defects in T-cell development are observed, including interrupted thymocytes differentiation and abnormal T-cell transcriptome.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002023991 – 764DNA-binding protein SATB1Add BLAST764

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki51Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei136N6-acetyllysineBy similarity1
Modified residuei185PhosphoserineBy similarity1
Modified residuei638PhosphoserineCombined sources1
Cross-linki745Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

Sumoylated. Sumoylation promotes cleavage by caspases.By similarity
Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation (By similarity).By similarity
Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms cannot dimerize and lose transcription regulation function because of impaired DNA and chromatin association.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei254 – 255Cleavage; by caspases2

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ60611
MaxQBiQ60611
PaxDbiQ60611
PRIDEiQ60611

PTM databases

iPTMnetiQ60611
PhosphoSitePlusiQ60611

Expressioni

Tissue specificityi

Mainly expressed in thymus, spleen, and lymph nodes with a lower level observed in the brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000023927 Expressed in 343 organ(s), highest expression level in thymus
CleanExiMM_SATB1
ExpressionAtlasiQ60611 baseline and differential
GenevisibleiQ60611 MM

Interactioni

Subunit structurei

Interacts with PCAF. Interacts with sumoylated PML and HDAC1 Tat via the PDZ-like dimerization domain. Interacts also with DYNLT3 and POLR2J2. Binds to EP300 (By similarity). Homodimer. Part of the nuclear protein complex gamma-globin promoter and enhancer binding factor (gamma-PE) composed at least of SATB1 and HOXB2. Interaction with CtBP1 when not acetylated stabalizes attachment to DNA and promotes transcription repression. Interacts with CUX1 (via DNA-binding domains); the interaction inhibits the attachment of both proteins to DNA.By similarity2 Publications

Protein-protein interaction databases

BioGridi203077, 1 interactor
STRINGi10090.ENSMUSP00000116006

Structurei

Secondary structure

1764
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ60611
SMRiQ60611
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni90 – 204PDZ-like dimerization domainAdd BLAST115
Regioni224 – 278Nuclear matrix targeting sequence (NMTS)By similarityAdd BLAST55
Regioni400 – 410Matrix attachment region (MAR) DNA-bindingBy similarityAdd BLAST11

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi20 – 40Nuclear localization signalBy similarityAdd BLAST21
Motifi139 – 143Protein interactionBy similarity5
Motifi224 – 278Nuclear matrix targeting sequence (NMTS)Add BLAST55

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi591 – 607Poly-GlnAdd BLAST17
Compositional biasi608 – 617Poly-Pro10

Sequence similaritiesi

Belongs to the CUT homeobox family.Curated

Keywords - Domaini

Homeobox, Repeat

Phylogenomic databases

eggNOGiKOG3755 Eukaryota
ENOG410YYG8 LUCA
GeneTreeiENSGT00390000008096
HOGENOMiHOG000004805
HOVERGENiHBG054240
InParanoidiQ60611
PhylomeDBiQ60611
TreeFamiTF332714

Family and domain databases

CDDicd00086 homeodomain, 1 hit
cd11585 SATB1_N, 1 hit
Gene3Di1.10.260.70, 1 hit
3.10.20.710, 1 hit
InterProiView protein in InterPro
IPR003350 CUT_dom
IPR032355 CUTL
IPR009057 Homeobox-like_sf
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
IPR038216 SATB_CUTL_sf
IPR038224 SATB_ULD_sf
IPR032392 ULD
PfamiView protein in Pfam
PF02376 CUT, 2 hits
PF16557 CUTL, 1 hit
PF00046 Homeobox, 1 hit
PF16534 ULD, 1 hit
SMARTiView protein in SMART
SM01109 CUT, 2 hits
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 2 hits
PROSITEiView protein in PROSITE
PS51042 CUT, 2 hits
PS50071 HOMEOBOX_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

Q60611-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDHLNEATQG KEHSEMSNNV SDPKGPPAKI ARLEQNGSPL GRGRLGSTGG
60 70 80 90 100
KMQGVPLKHS GHLMKTNLRK GTMLPVFCVV EHYENAIEYD CKEEHAEFVL
110 120 130 140 150
VRKDMLFNQL IEMALLSLGY SHSSAAQAKG LIQVGKWNPV PLSYVTDAPD
160 170 180 190 200
ATVADMLQDV YHVVTLKIQL HSCPKLEDLP PEQWSHTTVR NALKDLLKDM
210 220 230 240 250
NQSSLAKECP LSQSMISSIV NSTYYANVSA AKCQEFGRWY KHFKKTKDMM
260 270 280 290 300
VEMDSLSELS QQGANHVNFG QQPVPGNTAE QPPSPAQLSH GSQPSVRTPL
310 320 330 340 350
PNLHPGLVST PISPQLVNQQ LVMAQLLNQQ YAVNRLLAQQ SLNQQYLNHP
360 370 380 390 400
PPVSRSMNKP LEQQVSTNTE VSSEIYQWVR DELKRAGISQ AVFARVAFNR
410 420 430 440 450
TQGLLSEILR KEEDPKTASQ SLLVNLRAMQ NFLQLPEAER DRIYQDERER
460 470 480 490 500
SLNAASAMGP APLLSTPPSR PPQVKTATLA TERNGKPENN TMNINASIYD
510 520 530 540 550
EIQQEMKRAK VSQALFAKVA ATKSQGWLCE LLRWKEDPSP ENRTLWENLS
560 570 580 590 600
MIRRFLSLPQ PERDAIYEQE SNAVHHHGDR PPHIIHVPAE QIQQQQQQQQ
610 620 630 640 650
QQQQQQQPPP PPPQPQPQPQ AGPRLPPRQP TVASSAESDE ENRQKTRPRT
660 670 680 690 700
KISVEALGIL QSFIQDVGLY PDEEAIQTLS AQLDLPKYTI IKFFQNQRYY
710 720 730 740 750
LKHHGKLKDN SGLEVDVAEY KDEELLKDLE ESVQDKNANT LFSVKLEEEL
760
SVEGSTDVNA DLKD
Length:764
Mass (Da):85,880
Last modified:July 27, 2011 - v2
Checksum:i330ECCDBA8683B78
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PVB7E9PVB7_MOUSE
DNA-binding protein SATB
Satb1
795Annotation score:
D3YZD1D3YZD1_MOUSE
DNA-binding protein SATB1
Satb1
154Annotation score:
D3Z4F8D3Z4F8_MOUSE
DNA-binding protein SATB1
Satb1
74Annotation score:
D3YU76D3YU76_MOUSE
DNA-binding protein SATB1
Satb1
21Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti155D → N in AAA17372 (PubMed:8114718).Curated1
Sequence conflicti515L → P in AAA17372 (PubMed:8114718).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05252 mRNA Translation: AAA17372.1
AK088563 mRNA Translation: BAC40427.1
AK158518 mRNA Translation: BAE34542.1
CT010371 mRNA Translation: CAJ18578.1
BC011132 mRNA Translation: AAH11132.1
CH466559 Genomic DNA Translation: EDL23652.1
CCDSiCCDS28876.1
PIRiA56208
RefSeqiNP_001157102.1, NM_001163630.1
NP_001157103.1, NM_001163631.1
NP_001157104.1, NM_001163632.1
NP_033148.2, NM_009122.2
XP_017172852.1, XM_017317363.1
XP_017172853.1, XM_017317364.1
UniGeneiMm.311655

Genome annotation databases

EnsembliENSMUST00000129667; ENSMUSP00000116020; ENSMUSG00000023927
ENSMUST00000133574; ENSMUSP00000120536; ENSMUSG00000023927
ENSMUST00000144331; ENSMUSP00000116006; ENSMUSG00000023927
ENSMUST00000152830; ENSMUSP00000119842; ENSMUSG00000023927
ENSMUST00000169480; ENSMUSP00000128841; ENSMUSG00000023927
ENSMUST00000176669; ENSMUSP00000134957; ENSMUSG00000023927
GeneIDi20230
KEGGimmu:20230
UCSCiuc008czd.2 mouse

Keywords - Coding sequence diversityi

Chromosomal rearrangement

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05252 mRNA Translation: AAA17372.1
AK088563 mRNA Translation: BAC40427.1
AK158518 mRNA Translation: BAE34542.1
CT010371 mRNA Translation: CAJ18578.1
BC011132 mRNA Translation: AAH11132.1
CH466559 Genomic DNA Translation: EDL23652.1
CCDSiCCDS28876.1
PIRiA56208
RefSeqiNP_001157102.1, NM_001163630.1
NP_001157103.1, NM_001163631.1
NP_001157104.1, NM_001163632.1
NP_033148.2, NM_009122.2
XP_017172852.1, XM_017317363.1
XP_017172853.1, XM_017317364.1
UniGeneiMm.311655

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Q2JX-ray2.60A/B/C/D71-246[»]
ProteinModelPortaliQ60611
SMRiQ60611
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203077, 1 interactor
STRINGi10090.ENSMUSP00000116006

PTM databases

iPTMnetiQ60611
PhosphoSitePlusiQ60611

Proteomic databases

EPDiQ60611
MaxQBiQ60611
PaxDbiQ60611
PRIDEiQ60611

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000129667; ENSMUSP00000116020; ENSMUSG00000023927
ENSMUST00000133574; ENSMUSP00000120536; ENSMUSG00000023927
ENSMUST00000144331; ENSMUSP00000116006; ENSMUSG00000023927
ENSMUST00000152830; ENSMUSP00000119842; ENSMUSG00000023927
ENSMUST00000169480; ENSMUSP00000128841; ENSMUSG00000023927
ENSMUST00000176669; ENSMUSP00000134957; ENSMUSG00000023927
GeneIDi20230
KEGGimmu:20230
UCSCiuc008czd.2 mouse

Organism-specific databases

CTDi6304
MGIiMGI:105084 Satb1

Phylogenomic databases

eggNOGiKOG3755 Eukaryota
ENOG410YYG8 LUCA
GeneTreeiENSGT00390000008096
HOGENOMiHOG000004805
HOVERGENiHBG054240
InParanoidiQ60611
PhylomeDBiQ60611
TreeFamiTF332714

Enzyme and pathway databases

ReactomeiR-MMU-111465 Apoptotic cleavage of cellular proteins
R-MMU-4551638 SUMOylation of chromatin organization proteins

Miscellaneous databases

ChiTaRSiSatb1 mouse
PROiPR:Q60611
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023927 Expressed in 343 organ(s), highest expression level in thymus
CleanExiMM_SATB1
ExpressionAtlasiQ60611 baseline and differential
GenevisibleiQ60611 MM

Family and domain databases

CDDicd00086 homeodomain, 1 hit
cd11585 SATB1_N, 1 hit
Gene3Di1.10.260.70, 1 hit
3.10.20.710, 1 hit
InterProiView protein in InterPro
IPR003350 CUT_dom
IPR032355 CUTL
IPR009057 Homeobox-like_sf
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
IPR038216 SATB_CUTL_sf
IPR038224 SATB_ULD_sf
IPR032392 ULD
PfamiView protein in Pfam
PF02376 CUT, 2 hits
PF16557 CUTL, 1 hit
PF00046 Homeobox, 1 hit
PF16534 ULD, 1 hit
SMARTiView protein in SMART
SM01109 CUT, 2 hits
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 2 hits
PROSITEiView protein in PROSITE
PS51042 CUT, 2 hits
PS50071 HOMEOBOX_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSATB1_MOUSE
AccessioniPrimary (citable) accession number: Q60611
Secondary accession number(s): Q91XB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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