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Protein

2-oxoglutarate dehydrogenase, mitochondrial

Gene

Ogdh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex, which mediates the decarboxylation of alpha-ketoglutarate. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.By similarity

Catalytic activityi

2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2.By similarity

Cofactori

thiamine diphosphateBy similarity

Activity regulationi

Calcium ions and ADP stimulate, whereas ATP and NADH reduce catalytic activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi154CalciumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi154 – 158By similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandCalcium, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

ReactomeiR-MMU-389661 Glyoxylate metabolism and glycine degradation
R-MMU-71064 Lysine catabolism
R-MMU-71403 Citric acid cycle (TCA cycle)

Names & Taxonomyi

Protein namesi
Recommended name:
2-oxoglutarate dehydrogenase, mitochondrial (EC:1.2.4.2By similarity)
Alternative name(s):
2-oxoglutarate dehydrogenase complex component E1
Short name:
OGDC-E1
Alpha-ketoglutarate dehydrogenase
Gene namesi
Name:Ogdh
Synonyms:Kiaa4192
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1098267 Ogdh

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176831

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 40MitochondrionSequence analysisAdd BLAST40
ChainiPRO_000002043441 – 10232-oxoglutarate dehydrogenase, mitochondrialAdd BLAST983

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei74N6-succinyllysineCombined sources1
Modified residuei100PhosphoserineCombined sources1
Modified residuei401N6-acetyllysineCombined sources1
Cross-linki534Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei564N6-succinyllysineCombined sources1
Modified residuei970N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ60597
PaxDbiQ60597
PeptideAtlasiQ60597
PRIDEiQ60597

2D gel databases

REPRODUCTION-2DPAGEiQ60597

PTM databases

iPTMnetiQ60597
PhosphoSitePlusiQ60597
SwissPalmiQ60597

Expressioni

Gene expression databases

BgeeiENSMUSG00000020456 Expressed in 326 organ(s), highest expression level in heart
CleanExiMM_OGDH
ExpressionAtlasiQ60597 baseline and differential
GenevisibleiQ60597 MM

Interactioni

Subunit structurei

The 2-oxoglutarate dehydrogenase complex is composed of OGDH (2-oxoglutarate dehydrogenase; E1), DLST (dihydrolipoamide succinyltransferase; E2) and DLD (dihydrolipoamide dehydrogenase; E3). It contains multiple copies of the three enzymatic components (E1, E2 and E3). In the nucleus, the The 2-oxoglutarate dehydrogenase complex associates with KAT2A.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201905, 4 interactors
IntActiQ60597, 11 interactors
MINTiQ60597
STRINGi10090.ENSMUSP00000091041

Structurei

3D structure databases

ProteinModelPortaliQ60597
SMRiQ60597
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni933 – 939Recognized by alloreactive CD8 cytotoxic T-lymphocytes in association with a class I MHC protein7

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0450 Eukaryota
COG0567 LUCA
GeneTreeiENSGT00530000063092
HOVERGENiHBG001892
InParanoidiQ60597
KOiK00164
OMAiIDMVCYR
OrthoDBiEOG091G025G
PhylomeDBiQ60597
TreeFamiTF300695

Family and domain databases

InterProiView protein in InterPro
IPR032106 2-oxogl_dehyd_N
IPR011603 2oxoglutarate_DH_E1
IPR001017 DH_E1
IPR031717 KGD_C
IPR029061 THDP-binding
IPR005475 Transketolase-like_Pyr-bd
PANTHERiPTHR23152 PTHR23152, 1 hit
PfamiView protein in Pfam
PF16078 2-oxogl_dehyd_N, 1 hit
PF00676 E1_dh, 1 hit
PF16870 OxoGdeHyase_C, 1 hit
PF02779 Transket_pyr, 1 hit
PIRSFiPIRSF000157 Oxoglu_dh_E1, 1 hit
SMARTiView protein in SMART
SM00861 Transket_pyr, 1 hit
SUPFAMiSSF52518 SSF52518, 2 hits
TIGRFAMsiTIGR00239 2oxo_dh_E1, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q60597-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MFHLRTCAAK LRPLTASQTV KTFSQNKPAA IRTFQQIRCY SAPVAAEPFL
60 70 80 90 100
SGTSSNYVEE MYCAWLENPK SVHKSWDIFF RNTNAGAPPG TAYQSPLSLS
110 120 130 140 150
RSSLATMAHA QSLVEAQPNV DKLVEDHLAV QSLIRAYQIR GHHVAQLDPL
160 170 180 190 200
GILDADLDSS VPADIISSTD KLGFYGLHES DLDKVFHLPT TTFIGGQEPA
210 220 230 240 250
LPLREIIRRL EMAYCQHIGV EFMFINDLEQ CQWIRQKFET PGIMQFTNEE
260 270 280 290 300
KRTLLARLVR STRFEEFLQR KWSSEKRFGL EGCEVLIPAL KTIIDMSSAN
310 320 330 340 350
GVDYVIMGMP HRGRLNVLAN VIRKELEQIF CQFDSKLEAA DEGSGDMKYH
360 370 380 390 400
LGMYHRRINR VTDRNITLSL VANPSHLEAA DPVVMGKTKA EQFYCGDTEG
410 420 430 440 450
KKVMSILLHG DAAFAGQGIV YETFHLSDLP SYTTHGTVHV VVNNQIGFTT
460 470 480 490 500
DPRMARSSPY PTDVARVVNA PIFHVNSDDP EAVMYVCKVA AEWRNTFHKD
510 520 530 540 550
VVVDLVCYRR NGHNEMDEPM FTQPLMYKQI RKQKPVLQKY AELLVSQGVV
560 570 580 590 600
NQPEYEEEIS KYDKICEEAF TRSKDEKILH IKHWLDSPWP GFFTLDGQPR
610 620 630 640 650
SMTCPSTGLE EDVLFHIGKV ASSVPVENFT IHGGLSRILK TRRELVTNRT
660 670 680 690 700
VDWALAEYMA FGSLLKEGIH VRLSGQDVER GTFSHRHHVL HDQNVDKRTC
710 720 730 740 750
IPMNHLWPNQ APYTVCNSSL SEYGVLGFEL GFAMASPNAL VLWEAQFGDF
760 770 780 790 800
NNMAQCIIDQ FICPGQAKWV RQNGIVLLLP HGMEGMGPEH SSARPERFLQ
810 820 830 840 850
MCNDDPDVLP DLQEENFDIN QLYDCNWIVV NCSTPGNFFH VLRRQILLPF
860 870 880 890 900
RKPLIVFTPK SLLRHPEART SFDEMLPGTH FQRVIPENGP AAQDPHKVKR
910 920 930 940 950
LLFCTGKVYY DLTRERKARN MEEEVAITRI EQLSPFPFDL LLKEAQKYPN
960 970 980 990 1000
AELAWCQEEH KNQGYYDYVK PRLRTTIDRA KPVWYAGRDP AAAPATGNKK
1010 1020
THLTELQRFL DTAFDLDAFK KFS
Length:1,023
Mass (Da):116,449
Last modified:May 1, 2007 - v3
Checksum:iA0F3F8D36C7A76BC
GO
Isoform 2 (identifier: Q60597-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     139-172: IRGHHVAQLDPLGILDADLDSSVPADIISSTDKL → VRGHHIAKSCVNFDDAPVTVSSNV

Show »
Length:1,013
Mass (Da):115,419
Checksum:i64CBC6F0E46B5D5F
GO
Isoform 3 (identifier: Q60597-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     172-172: L → LDLAVFKERLRMLTVG

Note: No experimental confirmation available.
Show »
Length:1,038
Mass (Da):118,179
Checksum:i7DC3C47068B12D2C
GO
Isoform 4 (identifier: Q60597-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     139-172: IRGHHVAQLDPLGILDADLDSSVPADIISSTDKL → VRGHHIAKLD...KERLRMLTVG

Show »
Length:1,034
Mass (Da):117,758
Checksum:i7023215EEEE9516B
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Z4YJV4Z4YJV4_MOUSE
2-oxoglutarate dehydrogenase, mitoc...
Ogdh
1,019Annotation score:
Q5SVY0Q5SVY0_MOUSE
2-oxoglutarate dehydrogenase, mitoc...
Ogdh
123Annotation score:
Q5SVY1Q5SVY1_MOUSE
2-oxoglutarate dehydrogenase, mitoc...
Ogdh
84Annotation score:

Sequence cautioni

The sequence AAH31165 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAD90530 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti416G → V in AAH49104 (PubMed:15489334).Curated1
Sequence conflicti549V → F in AAH49104 (PubMed:15489334).Curated1
Sequence conflicti552Q → E in AAH57354 (PubMed:15489334).Curated1
Sequence conflicti576E → K in BAE29234 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024799139 – 172IRGHH…STDKL → VRGHHIAKSCVNFDDAPVTV SSNV in isoform 2. 1 PublicationAdd BLAST34
Alternative sequenceiVSP_024800139 – 172IRGHH…STDKL → VRGHHIAKLDPLGISCVNFD DAPVTVSSNVDLAVFKERLR MLTVG in isoform 4. 1 PublicationAdd BLAST34
Alternative sequenceiVSP_024801172L → LDLAVFKERLRMLTVG in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147289 mRNA Translation: BAE27824.1
AK150009 mRNA Translation: BAE29234.1
AK169286 mRNA Translation: BAE41044.1
AK220536 mRNA Translation: BAD90530.1 Different initiation.
AL607152 Genomic DNA No translation available.
BC025040 mRNA Translation: AAH25040.1
BC013670 mRNA Translation: AAH13670.1
BC029143 mRNA Translation: AAH29143.1
BC031165 mRNA Translation: AAH31165.1 Different initiation.
BC049104 mRNA Translation: AAH49104.1
BC057354 mRNA Translation: AAH57354.1
U02971 mRNA Translation: AAC52130.1
CCDSiCCDS36106.1 [Q60597-1]
CCDS56758.1 [Q60597-4]
PIRiI48884 A41911
RefSeqiNP_001239211.1, NM_001252282.1 [Q60597-3]
NP_001239212.1, NM_001252283.1 [Q60597-4]
NP_001239216.1, NM_001252287.1 [Q60597-1]
NP_001239217.1, NM_001252288.1
NP_035086.2, NM_010956.4 [Q60597-1]
XP_006514645.1, XM_006514582.2 [Q60597-4]
XP_006514646.1, XM_006514583.3
XP_006514647.1, XM_006514584.3 [Q60597-4]
XP_006514648.1, XM_006514585.2
XP_017169826.1, XM_017314337.1
UniGeneiMm.276348
Mm.479411
Mm.490272

Genome annotation databases

EnsembliENSMUST00000003461; ENSMUSP00000003461; ENSMUSG00000020456 [Q60597-1]
ENSMUST00000093350; ENSMUSP00000091041; ENSMUSG00000020456 [Q60597-4]
ENSMUST00000101554; ENSMUSP00000099090; ENSMUSG00000020456 [Q60597-1]
GeneIDi18293
KEGGimmu:18293
UCSCiuc007hyf.2 mouse [Q60597-1]
uc007hyg.2 mouse [Q60597-3]
uc007hyh.2 mouse [Q60597-4]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147289 mRNA Translation: BAE27824.1
AK150009 mRNA Translation: BAE29234.1
AK169286 mRNA Translation: BAE41044.1
AK220536 mRNA Translation: BAD90530.1 Different initiation.
AL607152 Genomic DNA No translation available.
BC025040 mRNA Translation: AAH25040.1
BC013670 mRNA Translation: AAH13670.1
BC029143 mRNA Translation: AAH29143.1
BC031165 mRNA Translation: AAH31165.1 Different initiation.
BC049104 mRNA Translation: AAH49104.1
BC057354 mRNA Translation: AAH57354.1
U02971 mRNA Translation: AAC52130.1
CCDSiCCDS36106.1 [Q60597-1]
CCDS56758.1 [Q60597-4]
PIRiI48884 A41911
RefSeqiNP_001239211.1, NM_001252282.1 [Q60597-3]
NP_001239212.1, NM_001252283.1 [Q60597-4]
NP_001239216.1, NM_001252287.1 [Q60597-1]
NP_001239217.1, NM_001252288.1
NP_035086.2, NM_010956.4 [Q60597-1]
XP_006514645.1, XM_006514582.2 [Q60597-4]
XP_006514646.1, XM_006514583.3
XP_006514647.1, XM_006514584.3 [Q60597-4]
XP_006514648.1, XM_006514585.2
XP_017169826.1, XM_017314337.1
UniGeneiMm.276348
Mm.479411
Mm.490272

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3E2HX-ray3.80Q932-940[»]
3TF7X-ray2.75B/F932-940[»]
ProteinModelPortaliQ60597
SMRiQ60597
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201905, 4 interactors
IntActiQ60597, 11 interactors
MINTiQ60597
STRINGi10090.ENSMUSP00000091041

Chemistry databases

ChEMBLiCHEMBL2176831

PTM databases

iPTMnetiQ60597
PhosphoSitePlusiQ60597
SwissPalmiQ60597

2D gel databases

REPRODUCTION-2DPAGEiQ60597

Proteomic databases

EPDiQ60597
PaxDbiQ60597
PeptideAtlasiQ60597
PRIDEiQ60597

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003461; ENSMUSP00000003461; ENSMUSG00000020456 [Q60597-1]
ENSMUST00000093350; ENSMUSP00000091041; ENSMUSG00000020456 [Q60597-4]
ENSMUST00000101554; ENSMUSP00000099090; ENSMUSG00000020456 [Q60597-1]
GeneIDi18293
KEGGimmu:18293
UCSCiuc007hyf.2 mouse [Q60597-1]
uc007hyg.2 mouse [Q60597-3]
uc007hyh.2 mouse [Q60597-4]

Organism-specific databases

CTDi4967
MGIiMGI:1098267 Ogdh
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0450 Eukaryota
COG0567 LUCA
GeneTreeiENSGT00530000063092
HOVERGENiHBG001892
InParanoidiQ60597
KOiK00164
OMAiIDMVCYR
OrthoDBiEOG091G025G
PhylomeDBiQ60597
TreeFamiTF300695

Enzyme and pathway databases

ReactomeiR-MMU-389661 Glyoxylate metabolism and glycine degradation
R-MMU-71064 Lysine catabolism
R-MMU-71403 Citric acid cycle (TCA cycle)

Miscellaneous databases

ChiTaRSiOgdh mouse
PROiPR:Q60597
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020456 Expressed in 326 organ(s), highest expression level in heart
CleanExiMM_OGDH
ExpressionAtlasiQ60597 baseline and differential
GenevisibleiQ60597 MM

Family and domain databases

InterProiView protein in InterPro
IPR032106 2-oxogl_dehyd_N
IPR011603 2oxoglutarate_DH_E1
IPR001017 DH_E1
IPR031717 KGD_C
IPR029061 THDP-binding
IPR005475 Transketolase-like_Pyr-bd
PANTHERiPTHR23152 PTHR23152, 1 hit
PfamiView protein in Pfam
PF16078 2-oxogl_dehyd_N, 1 hit
PF00676 E1_dh, 1 hit
PF16870 OxoGdeHyase_C, 1 hit
PF02779 Transket_pyr, 1 hit
PIRSFiPIRSF000157 Oxoglu_dh_E1, 1 hit
SMARTiView protein in SMART
SM00861 Transket_pyr, 1 hit
SUPFAMiSSF52518 SSF52518, 2 hits
TIGRFAMsiTIGR00239 2oxo_dh_E1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiODO1_MOUSE
AccessioniPrimary (citable) accession number: Q60597
Secondary accession number(s): Q3UDM7
, Q5DTI4, Q5SVX7, Q5SVX9, Q6PFZ2, Q80Y57, Q8K0K7, Q8K2Z3, Q8R3M2, Q91WP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 2007
Last modified: October 10, 2018
This is version 170 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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