UniProtKB - Q60591 (NFAC2_MOUSE)
Nuclear factor of activated T-cells, cytoplasmic 2
Nfatc2
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 423 – 430 | 8 |
GO - Molecular functioni
- chromatin binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: BHF-UCL
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- phosphatase binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription factor binding Source: MGI
- transcription regulatory region sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- B cell receptor signaling pathway Source: MGI
- calcineurin-NFAT signaling cascade Source: MGI
- cell migration Source: UniProtKB
- cellular response to DNA damage stimulus Source: MGI
- myotube cell development Source: MGI
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- negative regulation of vascular associated smooth muscle cell differentiation Source: MGI
- positive regulation of B cell proliferation Source: MGI
- positive regulation of gene expression Source: UniProtKB
- positive regulation of myoblast fusion Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to drug Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-2025928, Calcineurin activates NFAT R-MMU-2871809, FCERI mediated Ca+2 mobilization R-MMU-5607763, CLEC7A (Dectin-1) induces NFAT activation |
Protein family/group databases
MoonDBi | Q60591, Predicted |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear factor of activated T-cells, cytoplasmic 2Short name: NF-ATc2 Short name: NFATc2 Alternative name(s): NFAT pre-existing subunit Short name: NF-ATp T-cell transcription factor NFAT1 |
Gene namesi | Name:Nfatc2 Synonyms:Nfat1, Nfatp |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:102463, Nfatc2 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Note: Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- chromatin Source: BHF-UCL
- cytoplasm Source: MGI
- ribonucleoprotein complex Source: MGI
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 112 | R → A: Lowers dephosphorylation. 1 Publication | 1 | |
Mutagenesisi | 114 | E → A: Lowers dephosphorylation. 1 Publication | 1 | |
Mutagenesisi | 116 | T → A: No dephosphorylation. 1 Publication | 1 | |
Mutagenesisi | 164 | R → A: Induces aberrant nuclear localization of the phosphorylated form. 1 Publication | 1 | |
Mutagenesisi | 423 | R → A: Decrease in binding to DNA. 1 Publication | 1 | |
Mutagenesisi | 425 | H → A: No change in binding to DNA. 1 Publication | 1 | |
Mutagenesisi | 426 | Y → A: Decrease in binding to DNA. 1 Publication | 1 | |
Mutagenesisi | 428 | T → A: No change in binding to DNA. 1 Publication | 1 | |
Mutagenesisi | 428 | T → C: No change in binding to DNA and confers DNA-binding sensitivity to sulfhydryl modifications. 1 Publication | 1 | |
Mutagenesisi | 429 | E → A: Decrease in binding to DNA. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000205179 | 1 – 927 | Nuclear factor of activated T-cells, cytoplasmic 2Add BLAST | 927 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 23 | PhosphoserineBy similarity | 1 | |
Modified residuei | 53 | PhosphoserineCurated | 1 | |
Modified residuei | 54 | PhosphoserineCurated | 1 | |
Modified residuei | 56 | PhosphoserineCurated | 1 | |
Modified residuei | 99 | Phosphoserine1 Publication | 1 | |
Modified residuei | 107 | PhosphoserineBy similarity | 1 | |
Modified residuei | 110 | PhosphoserineBy similarity | 1 | |
Modified residuei | 136 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 150 | PhosphoserineBy similarity | 1 | |
Modified residuei | 170 | Phosphoserine1 Publication | 1 | |
Modified residuei | 173 | Phosphoserine1 Publication | 1 | |
Modified residuei | 174 | Phosphoserine1 Publication | 1 | |
Modified residuei | 176 | Phosphoserine1 Publication | 1 | |
Modified residuei | 177 | Phosphoserine1 Publication | 1 | |
Modified residuei | 179 | Phosphoserine1 Publication | 1 | |
Modified residuei | 182 | Phosphoserine1 Publication | 1 | |
Modified residuei | 215 | Phosphoserine1 Publication | 1 | |
Modified residuei | 219 | Phosphoserine1 Publication | 1 | |
Modified residuei | 223 | Phosphoserine1 Publication | 1 | |
Modified residuei | 238 | Phosphoserine1 Publication | 1 | |
Modified residuei | 245 | Phosphoserine1 Publication | 1 | |
Modified residuei | 257 | PhosphoserineCombined sources | 1 | |
Modified residuei | 270 | Phosphoserine1 Publication | 1 | |
Modified residuei | 276 | Phosphoserine1 Publication | 1 | |
Modified residuei | 278 | Phosphoserine1 Publication | 1 | |
Modified residuei | 282 | Phosphoserine1 Publication | 1 | |
Modified residuei | 328 | Phosphoserine1 Publication | 1 | |
Modified residuei | 365 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 757 | PhosphoserineBy similarity | 1 | |
Modified residuei | 759 | PhosphoserineBy similarity | 1 | |
Modified residuei | 761 | PhosphoserineBy similarity | 1 | |
Modified residuei | 860 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q60591 |
jPOSTi | Q60591 |
PaxDbi | Q60591 |
PeptideAtlasi | Q60591 |
PRIDEi | Q60591 |
ProteomicsDBi | 287400 [Q60591-3] 287401 [Q60591-2] 287402 [Q60591-4] |
PTM databases
iPTMneti | Q60591 |
PhosphoSitePlusi | Q60591 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000027544, Expressed in lumbar subsegment of spinal cord and 251 other tissues |
ExpressionAtlasi | Q60591, baseline and differential |
Genevisiblei | Q60591, MM |
Interactioni
Subunit structurei
Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. The phosphorylated form specifically interacts with XPO1; which mediates nuclear export. NFATC proteins bind to DNA as monomers.
Interacts with NFATC2IP.
Interacts with FOXP3 (By similarity).
Interacts with TBX21 ('Thr-302' phosphorylated form) (PubMed:23616576).
Interacts with KAT2A (PubMed:28424240).
Interacts with HOMER2 and HOMER3; this interaction competes with calcineurin/PPP3CA-binding and hence prevents NFATC2 dephosphorylation and activation (By similarity).
Interacts with protein phosphatase PPP3CA/calcineurin A (By similarity).
By similarity4 PublicationsBinary interactionsi
Q60591
With | #Exp. | IntAct |
---|---|---|
VRK2 - isoform 1 [Q86Y07-1] from Homo sapiens. | 2 | EBI-643104,EBI-1207633 |
GO - Molecular functioni
- phosphatase binding Source: MGI
- transcription factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201739, 2 interactors |
ComplexPortali | CPX-610, AP-1 transcription factor complex FOS-JUN-NFATC2 |
CORUMi | Q60591 |
DIPi | DIP-49476N |
ELMi | Q60591 |
IntActi | Q60591, 11 interactors |
STRINGi | 10090.ENSMUSP00000074198 |
Miscellaneous databases
RNActi | Q60591, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 186 – 202 | 1Add BLAST | 17 | |
Repeati | 215 – 231 | 2Add BLAST | 17 | |
Repeati | 274 – 290 | 3; approximateAdd BLAST | 17 | |
Domaini | 394 – 576 | RHDPROSITE-ProRule annotationAdd BLAST | 183 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 111 – 116 | Calcineurin-bindingBy similarity | 6 | |
Regioni | 119 – 201 | Trans-activation domain A (TAD-A)Add BLAST | 83 | |
Regioni | 163 – 177 | Required for cytoplasmic retention of the phosphorylated formAdd BLAST | 15 | |
Regioni | 186 – 292 | 3 X approximate SP repeatsAdd BLAST | 107 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 253 – 255 | Nuclear localization signal | 3 |
Domaini
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502QTJI, Eukaryota |
GeneTreei | ENSGT00940000156230 |
HOGENOMi | CLU_010185_1_0_1 |
InParanoidi | Q60591 |
OMAi | CENFPPG |
TreeFami | TF326480 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit 2.60.40.340, 1 hit |
InterProi | View protein in InterPro IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR002909, IPT_dom IPR008366, NFAT IPR008967, p53-like_TF_DNA-bd IPR032397, RHD_dimer IPR011539, RHD_DNA_bind_dom IPR037059, RHD_DNA_bind_dom_sf |
PANTHERi | PTHR12533, PTHR12533, 1 hit |
Pfami | View protein in Pfam PF16179, RHD_dimer, 1 hit PF00554, RHD_DNA_bind, 1 hit |
PRINTSi | PR01789, NUCFACTORATC |
SMARTi | View protein in SMART SM00429, IPT, 1 hit |
SUPFAMi | SSF49417, SSF49417, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS50254, REL_2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDVPEPQPDP DGGDGPGHEP GGSPQDELDF SILFDYDYLN PIEEEPIAHK
60 70 80 90 100
AISSPSGLAY PDDVLDYGLK PCNPLASLSG EPPGRFGEPD SIGFQNFLSP
110 120 130 140 150
VKPAGASGPS PRIEITPSHE LMQAGGALRG RDAGLSPEQP ALALAGVAAS
160 170 180 190 200
PRFTLPVPGY EGYREPLCLS PASSGSSASF ISDTFSPYTS PCVSPNNAGP
210 220 230 240 250
DDLCPQFQNI PAHYSPRTSP IMSPRTSLAE DSCLGRHSPV PRPASRSSSP
260 270 280 290 300
GAKRRHSCAE ALVAPLPAAS PQRSRSPSPQ PSPHVALQDD SIPAGYPPTA
310 320 330 340 350
GSAVLMDALN TLATDSPCGI PSKIWKTSPD PTPVSTAPSK AGLARHIYPT
360 370 380 390 400
VEFLGPCEQE ERRNSAPESI LLVPPTWPKQ LVPAIPICSI PVTASLPPLE
410 420 430 440 450
WPLSNQSGSY ELRIEVQPKP HHRAHYETEG SRGAVKAPTG GHPVVQLHGY
460 470 480 490 500
MENKPLGLQI FIGTADERIL KPHAFYQVHR ITGKTVTTTS YEKIVGNTKV
510 520 530 540 550
LEIPLEPKNN MRATIDCAGI LKLRNADIEL RKGETDIGRK NTRVRLVFRV
560 570 580 590 600
HVPEPSGRIV SLQAASNPIE CSQRSAHELP MVERQDMDSC LVYGGQQMIL
610 620 630 640 650
TGQNFTAESK VVFMEKTTDG QQIWEMEATV DKDKSQPNML FVEIPEYRNK
660 670 680 690 700
HIRVPVKVNF YVINGKRKRS QPQHFTYHPV PAIKTEPSDE YEPSLICSPA
710 720 730 740 750
HGGLGSQPYY PQHPMLAESP SCLVATMAPC QQFRSGLSSP DARYQQQSPA
760 770 780 790 800
AALYQRSKSL SPGLLGYQQP SLLAAPLGLA DAHRSVLVHA GSQGQGQGST
810 820 830 840 850
LPHTSSASQQ ASPVIHYSPT NQQLRGGGHQ EFQHIMYCEN FGPSSARPGP
860 870 880 890 900
PPINQGQRLS PGAYPTVIQQ QTAPSQRAAK NGPSDQKEAL PTGVTVKQEQ
910 920
NLDQTYLDDV NEIIRKEFSG PPSRNQT
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2AQC8 | A2AQC8_MOUSE | Nuclear factor of activated T-cells... | Nfatc2 | 653 | Annotation score: | ||
B5B2R5 | B5B2R5_MOUSE | Nuclear factor of activated T-cells... | Nfatc2 | 673 | Annotation score: | ||
Q8C443 | Q8C443_MOUSE | Nuclear factor of activated T-cells... | Nfatc2 | 452 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 78 | L → P in AAK49895 (PubMed:11278367).Curated | 1 | |
Sequence conflicti | 287 | L → P in AAC52929 (PubMed:8668213).Curated | 1 | |
Sequence conflicti | 287 | L → P in AAC52930 (PubMed:8668213).Curated | 1 | |
Sequence conflicti | 287 | L → P in AAC52931 (PubMed:8668213).Curated | 1 | |
Sequence conflicti | 802 | P → R in AAC52929 (PubMed:8668213).Curated | 1 | |
Sequence conflicti | 802 | P → R in AAC52930 (PubMed:8668213).Curated | 1 | |
Sequence conflicti | 802 | P → R in AAC52931 (PubMed:8668213).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_005597 | 619 – 806 | DGQQI…PHTSS → GPAGTCETRPLPISLISADR LSPWLSRLQRNPPGSVFRCS VLLPAPGSSLVLLAL in isoform D. 1 PublicationAdd BLAST | 188 | |
Alternative sequenceiVSP_005596 | 910 – 927 | VNEII…SRNQT → ELIDTHLSWIQNIL in isoform B. 1 PublicationAdd BLAST | 18 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U02079 mRNA Translation: AAC52929.1 Sequence problems. U36575 mRNA Translation: AAC52930.1 U36576 mRNA Translation: AAC52931.1 AF289078 mRNA Translation: AAK49895.1 EU887588 mRNA Translation: ACG55608.1 AL840639 Genomic DNA No translation available. AL844575 Genomic DNA No translation available. |
CCDSi | CCDS17112.1 [Q60591-3] CCDS50803.1 [Q60591-2] |
PIRi | A48753 |
RefSeqi | NP_035029.2, NM_010899.3 [Q60591-3] |
Genome annotation databases
Ensembli | ENSMUST00000074618; ENSMUSP00000074198; ENSMUSG00000027544 [Q60591-3] ENSMUST00000109184; ENSMUSP00000104812; ENSMUSG00000027544 [Q60591-2] |
GeneIDi | 18019 |
KEGGi | mmu:18019 |
UCSCi | uc008oau.2, mouse [Q60591-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U02079 mRNA Translation: AAC52929.1 Sequence problems. U36575 mRNA Translation: AAC52930.1 U36576 mRNA Translation: AAC52931.1 AF289078 mRNA Translation: AAK49895.1 EU887588 mRNA Translation: ACG55608.1 AL840639 Genomic DNA No translation available. AL844575 Genomic DNA No translation available. |
CCDSi | CCDS17112.1 [Q60591-3] CCDS50803.1 [Q60591-2] |
PIRi | A48753 |
RefSeqi | NP_035029.2, NM_010899.3 [Q60591-3] |
3D structure databases
SMRi | Q60591 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201739, 2 interactors |
ComplexPortali | CPX-610, AP-1 transcription factor complex FOS-JUN-NFATC2 |
CORUMi | Q60591 |
DIPi | DIP-49476N |
ELMi | Q60591 |
IntActi | Q60591, 11 interactors |
STRINGi | 10090.ENSMUSP00000074198 |
Protein family/group databases
MoonDBi | Q60591, Predicted |
PTM databases
iPTMneti | Q60591 |
PhosphoSitePlusi | Q60591 |
Proteomic databases
EPDi | Q60591 |
jPOSTi | Q60591 |
PaxDbi | Q60591 |
PeptideAtlasi | Q60591 |
PRIDEi | Q60591 |
ProteomicsDBi | 287400 [Q60591-3] 287401 [Q60591-2] 287402 [Q60591-4] |
Protocols and materials databases
Antibodypediai | 1758, 525 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000074618; ENSMUSP00000074198; ENSMUSG00000027544 [Q60591-3] ENSMUST00000109184; ENSMUSP00000104812; ENSMUSG00000027544 [Q60591-2] |
GeneIDi | 18019 |
KEGGi | mmu:18019 |
UCSCi | uc008oau.2, mouse [Q60591-3] |
Organism-specific databases
CTDi | 4773 |
MGIi | MGI:102463, Nfatc2 |
Phylogenomic databases
eggNOGi | ENOG502QTJI, Eukaryota |
GeneTreei | ENSGT00940000156230 |
HOGENOMi | CLU_010185_1_0_1 |
InParanoidi | Q60591 |
OMAi | CENFPPG |
TreeFami | TF326480 |
Enzyme and pathway databases
Reactomei | R-MMU-2025928, Calcineurin activates NFAT R-MMU-2871809, FCERI mediated Ca+2 mobilization R-MMU-5607763, CLEC7A (Dectin-1) induces NFAT activation |
Miscellaneous databases
BioGRID-ORCSi | 18019, 4 hits in 52 CRISPR screens |
ChiTaRSi | Nfatc2, mouse |
PROi | PR:Q60591 |
RNActi | Q60591, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027544, Expressed in lumbar subsegment of spinal cord and 251 other tissues |
ExpressionAtlasi | Q60591, baseline and differential |
Genevisiblei | Q60591, MM |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit 2.60.40.340, 1 hit |
InterProi | View protein in InterPro IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR002909, IPT_dom IPR008366, NFAT IPR008967, p53-like_TF_DNA-bd IPR032397, RHD_dimer IPR011539, RHD_DNA_bind_dom IPR037059, RHD_DNA_bind_dom_sf |
PANTHERi | PTHR12533, PTHR12533, 1 hit |
Pfami | View protein in Pfam PF16179, RHD_dimer, 1 hit PF00554, RHD_DNA_bind, 1 hit |
PRINTSi | PR01789, NUCFACTORATC |
SMARTi | View protein in SMART SM00429, IPT, 1 hit |
SUPFAMi | SSF49417, SSF49417, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS50254, REL_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NFAC2_MOUSE | |
Accessioni | Q60591Primary (citable) accession number: Q60591 Secondary accession number(s): A2APK2 Q91Y65 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 197 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot