Nfatc2

Functionⁱ

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.By similarity

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
DNA bindingⁱ	423 – 430			8

GO - Molecular functionⁱ

chromatin binding
Source: MGI
Inferred from direct assayⁱ
- "A new role for the calcineurin/NFAT pathway in neonatal myosin heavy chain expression via the NFATc2/MyoD complex during mouse myogenesis."
  Daou N., Lecolle S., Lefebvre S., della Gaspera B., Charbonnier F., Chanoine C., Armand A.S.
  Development 140:4914-4925(2013) [PubMed] [Europe PMC] [Abstract]
DNA-binding transcription activator activity, RNA polymerase II-specific
Source: MGI
Inferred from direct assayⁱ
- Ref.1
  "Isolation of the cyclosporin-sensitive T cell transcription factor NFATp."
  McCaffrey P.G., Luo C., Kerpolla T.K., Jain J., Badalian T.M., Ho A.M., Burgeon E., Lane W.S., Lambert J.N., Curran T., Verdine G.L., Rao A., Hogan P.G.
  Science 262:750-754(1993) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE (ISOFORM A).
DNA-binding transcription factor activity
Source: MGI
Inferred from direct assayⁱ
- "Nuclear factor of activated T cells (NFAT) is involved in the depolarization-induced activation of growth hormone-releasing hormone gene transcription in vitro."
  Asai M., Iwasaki Y., Yoshida M., Mutsuga-Nakayama N., Arima H., Ito M., Takano K., Oiso Y.
  Mol. Endocrinol. 18:3011-3019(2004) [PubMed] [Europe PMC] [Abstract]
DNA-binding transcription factor activity, RNA polymerase II-specific
Source: BHF-UCL
Inferred from direct assayⁱ
- "Nfat and miR-25 cooperate to reactivate the transcription factor Hand2 in heart failure."
  Dirkx E., Gladka M.M., Philippen L.E., Armand A.S., Kinet V., Leptidis S., El Azzouzi H., Salic K., Bourajjaj M., da Silva G.J., Olieslagers S., van der Nagel R., de Weger R., Bitsch N., Kisters N., Seyen S., Morikawa Y., Chanoine C.
  De Windt L.J.
  Nat. Cell Biol. 15:1282-1293(2013) [PubMed] [Europe PMC] [Abstract]
DNA-binding transcription repressor activity, RNA polymerase II-specific
Source: NTNU_SB
Inferred from direct assayⁱ
- "NFATc2-mediated repression of cyclin-dependent kinase 4 expression."
  Baksh S., Widlund H.R., Frazer-Abel A.A., Du J., Fosmire S., Fisher D.E., DeCaprio J.A., Modiano J.F., Burakoff S.J.
  Mol. Cell 10:1071-1081(2002) [PubMed] [Europe PMC] [Abstract]
phosphatase binding Source: MGI
RNA polymerase II cis-regulatory region sequence-specific DNA binding
Source: NTNU_SB
Inferred from direct assayⁱ
- "NFATc2-mediated repression of cyclin-dependent kinase 4 expression."
  Baksh S., Widlund H.R., Frazer-Abel A.A., Du J., Fosmire S., Fisher D.E., DeCaprio J.A., Modiano J.F., Burakoff S.J.
  Mol. Cell 10:1071-1081(2002) [PubMed] [Europe PMC] [Abstract]
sequence-specific DNA binding
Source: MGI
Inferred from direct assayⁱ
- "NFATc3, a lymphoid-specific NFATc family member that is calcium-regulated and exhibits distinct DNA binding specificity."
  Ho S.N., Thomas D.J., Timmerman L.A., Li X., Francke U., Crabtree G.R.
  J. Biol. Chem. 270:19898-19907(1995) [PubMed] [Europe PMC] [Abstract]
- "A gene for a novel zinc-finger protein expressed in differentiated epithelial cells and transiently in certain mesenchymal cells."
  Garrett-Sinha L.A., Eberspaecher H., Seldin M.F., de Crombrugghe B.
  J. Biol. Chem. 271:31384-31390(1996) [PubMed] [Europe PMC] [Abstract]
transcription factor binding
Source: MGI
Inferred from physical interactionⁱ
- "A new role for the calcineurin/NFAT pathway in neonatal myosin heavy chain expression via the NFATc2/MyoD complex during mouse myogenesis."
  Daou N., Lecolle S., Lefebvre S., della Gaspera B., Charbonnier F., Chanoine C., Armand A.S.
  Development 140:4914-4925(2013) [PubMed] [Europe PMC] [Abstract]
transcription regulatory region DNA binding
Source: UniProtKB
Inferred from direct assayⁱ
- "An innate immunity signaling process suppresses macrophage ABCA1 expression through IRAK-1-mediated downregulation of retinoic acid receptor alpha and NFATc2."
  Maitra U., Parks J.S., Li L.
  Mol. Cell. Biol. 29:5989-5997(2009) [PubMed] [Europe PMC] [Abstract]
transcription regulatory region sequence-specific DNA binding
Source: BHF-UCL
Inferred from direct assayⁱ
- "Nfat and miR-25 cooperate to reactivate the transcription factor Hand2 in heart failure."
  Dirkx E., Gladka M.M., Philippen L.E., Armand A.S., Kinet V., Leptidis S., El Azzouzi H., Salic K., Bourajjaj M., da Silva G.J., Olieslagers S., van der Nagel R., de Weger R., Bitsch N., Kisters N., Seyen S., Morikawa Y., Chanoine C.
  De Windt L.J.
  Nat. Cell Biol. 15:1282-1293(2013) [PubMed] [Europe PMC] [Abstract]

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

Reactomeⁱ	R-MMU-2025928 Calcineurin activates NFAT R-MMU-2871809 FCERI mediated Ca+2 mobilization R-MMU-5607763 CLEC7A (Dectin-1) induces NFAT activation

Reactomeⁱ

R-MMU-2025928 Calcineurin activates NFAT
R-MMU-2871809 FCERI mediated Ca+2 mobilization
R-MMU-5607763 CLEC7A (Dectin-1) induces NFAT activation

Protein family/group databases

MoonDBⁱ	Q60591 Predicted

MoonDBⁱ

Q60591 Predicted

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Nuclear factor of activated T-cells, cytoplasmic 2 Short name: NF-ATc2 Short name: NFATc2 Alternative name(s): NFAT pre-existing subunit Short name: NF-ATp T-cell transcription factor NFAT1
Gene namesⁱ	Name:Nfatc2 Synonyms:Nfat1, Nfatp
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 2

Organism-specific databases

MGIⁱ	MGI:102463 Nfatc2

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	112	R → A: Lowers dephosphorylation. 1 Publication	1
Mutagenesisⁱ	114	E → A: Lowers dephosphorylation. 1 Publication	1
Mutagenesisⁱ	116	T → A: No dephosphorylation. 1 Publication	1
Mutagenesisⁱ	164	R → A: Induces aberrant nuclear localization of the phosphorylated form. 1 Publication	1
Mutagenesisⁱ	423	R → A: Decrease in binding to DNA. 1 Publication	1
Mutagenesisⁱ	425	H → A: No change in binding to DNA. 1 Publication	1
Mutagenesisⁱ	426	Y → A: Decrease in binding to DNA. 1 Publication	1
Mutagenesisⁱ	428	T → A: No change in binding to DNA. 1 Publication	1
Mutagenesisⁱ	428	T → C: No change in binding to DNA and confers DNA-binding sensitivity to sulfhydryl modifications. 1 Publication	1
Mutagenesisⁱ	429	E → A: Decrease in binding to DNA. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000205179	1 – 927	Nuclear factor of activated T-cells, cytoplasmic 2Add BLAST		927

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	23	PhosphoserineBy similarity	1
Modified residueⁱ	53	PhosphoserineCurated	1
Modified residueⁱ	54	PhosphoserineCurated	1
Modified residueⁱ	56	PhosphoserineCurated	1
Modified residueⁱ	99	Phosphoserine1 Publication	1
Modified residueⁱ	107	PhosphoserineBy similarity	1
Modified residueⁱ	110	PhosphoserineBy similarity	1
Modified residueⁱ	136	PhosphoserineCombined sources1 Publication	1
Modified residueⁱ	150	PhosphoserineBy similarity	1
Modified residueⁱ	170	Phosphoserine1 Publication	1
Modified residueⁱ	173	Phosphoserine1 Publication	1
Modified residueⁱ	174	Phosphoserine1 Publication	1
Modified residueⁱ	176	Phosphoserine1 Publication	1
Modified residueⁱ	177	Phosphoserine1 Publication	1
Modified residueⁱ	179	Phosphoserine1 Publication	1
Modified residueⁱ	182	Phosphoserine1 Publication	1
Modified residueⁱ	215	Phosphoserine1 Publication	1
Modified residueⁱ	219	Phosphoserine1 Publication	1
Modified residueⁱ	223	Phosphoserine1 Publication	1
Modified residueⁱ	238	Phosphoserine1 Publication	1
Modified residueⁱ	245	Phosphoserine1 Publication	1
Modified residueⁱ	257	PhosphoserineCombined sources	1
Modified residueⁱ	270	Phosphoserine1 Publication	1
Modified residueⁱ	276	Phosphoserine1 Publication	1
Modified residueⁱ	278	Phosphoserine1 Publication	1
Modified residueⁱ	282	Phosphoserine1 Publication	1
Modified residueⁱ	328	Phosphoserine1 Publication	1
Modified residueⁱ	365	PhosphoserineCombined sources1 Publication	1
Modified residueⁱ	757	PhosphoserineBy similarity	1
Modified residueⁱ	759	PhosphoserineBy similarity	1
Modified residueⁱ	761	PhosphoserineBy similarity	1
Modified residueⁱ	860	PhosphoserineBy similarity	1

Post-translational modificationⁱ

In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-365 region. Upon cell stimulation, all these sites except Ser-245 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, one site among Ser-53; Ser-54 and Ser-56 is phosphorylated; which is required for full transcriptional activity.1 Publication

Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome.By similarity

Keywords - PTMⁱ

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q60591
jPOSTⁱ	Q60591
PaxDbⁱ	Q60591
PeptideAtlas More...PeptideAtlasⁱ	Q60591
PRIDEⁱ	Q60591

PTM databases

iPTMnetⁱ	Q60591
PhosphoSitePlusⁱ	Q60591

Expressionⁱ

Tissue specificityⁱ

Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.1 Publication

Developmental stageⁱ

Expressed in the developing heart at 13.5 and 16.5 dpc, during the transition from spongy to compact myocardium.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000027544 Expressed in lumbar subsegment of spinal cord and 228 other tissues
ExpressionAtlasⁱ	Q60591 baseline and differential
Genevisibleⁱ	Q60591 MM

Interactionⁱ

Subunit structureⁱ

Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. The phosphorylated form specifically interacts with XPO1; which mediates nuclear export. NFATC proteins bind to DNA as monomers.

Interacts with NFATC2IP.

Interacts with FOXP3 (By similarity).

Interacts with TBX21 ('Thr-302' phosphorylated form) (PubMed:23616576).

Interacts with KAT2A (PubMed:28424240).

Interacts with HOMER2 and HOMER3; this interaction competes with calcineurin/PPP3CA-binding and hence prevents NFATC2 dephosphorylation and activation (By similarity).

Interacts with protein phosphatase PPP3CA/calcineurin A (By similarity).

By similarity4 Publications

Binary interactionsⁱ

Q60591

With	#Exp.	IntAct
VRK2 - isoform 1 [Q86Y07-1] from Homo sapiens.	2	EBI-643104,EBI-1207633

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGRIDⁱ	201739, 2 interactors
ComplexPortalⁱ	CPX-610 AP-1 transcription factor complex FOS-JUN-NFATC2
CORUMⁱ	Q60591
Database of interacting proteins More...DIPⁱ	DIP-49476N
ELMⁱ	Q60591
IntActⁱ	Q60591, 11 interactors
STRINGⁱ	10090.ENSMUSP00000074198

Miscellaneous databases

RNActⁱ	Q60591 protein

RNActⁱ

Q60591 protein

Structureⁱ

3D structure databases

SMRⁱ	Q60591
ModBaseⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Repeatⁱ	186 – 202	1Add BLAST	17
Repeatⁱ	215 – 231	2Add BLAST	17
Repeatⁱ	274 – 290	3; approximateAdd BLAST	17
Domainⁱ	394 – 576	RHDPROSITE-ProRule annotationAdd BLAST	183

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	111 – 116	Calcineurin-bindingBy similarity	6
Regionⁱ	119 – 201	Trans-activation domain A (TAD-A)Add BLAST	83
Regionⁱ	163 – 177	Required for cytoplasmic retention of the phosphorylated formAdd BLAST	15
Regionⁱ	186 – 292	3 X approximate SP repeatsAdd BLAST	107

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	253 – 255	Nuclear localization signal		3

Domainⁱ

Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.By similarity

Keywords - Domainⁱ

Repeat

Phylogenomic databases

eggNOGⁱ	ENOG410IHFR Eukaryota ENOG41105U2 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00940000156230
HOGENOMⁱ	CLU_010185_1_0_1
InParanoidⁱ	Q60591
KEGG Orthology (KO) More...KOⁱ	K17332
OMAⁱ	CENFPPG
TreeFamⁱ	TF326480

Family and domain databases

Gene3Dⁱ	2.60.40.10, 1 hit 2.60.40.340, 1 hit
InterProⁱ	View protein in InterPro IPR013783 Ig-like_fold IPR014756 Ig_E-set IPR002909 IPT_dom IPR008366 NFAT IPR008967 p53-like_TF_DNA-bd IPR032397 RHD_dimer IPR011539 RHD_DNA_bind_dom IPR037059 RHD_DNA_bind_dom_sf
PANTHERⁱ	PTHR12533 PTHR12533, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF16179 RHD_dimer, 1 hit PF00554 RHD_DNA_bind, 1 hit
PRINTSⁱ	PR01789 NUCFACTORATC
SMARTⁱ	View protein in SMART SM00429 IPT, 1 hit
SUPFAMⁱ	SSF49417 SSF49417, 1 hit SSF81296 SSF81296, 1 hit
PROSITEⁱ	View protein in PROSITE PS50254 REL_2, 1 hit

Sequences (4+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 4 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show all Align All

Isoform C (identifier: Q60591-3) [UniParc]FASTA Add to basket

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDVPEPQPDP DGGDGPGHEP GGSPQDELDF SILFDYDYLN PIEEEPIAHK 
        60         70         80         90        100
AISSPSGLAY PDDVLDYGLK PCNPLASLSG EPPGRFGEPD SIGFQNFLSP 
       110        120        130        140        150
VKPAGASGPS PRIEITPSHE LMQAGGALRG RDAGLSPEQP ALALAGVAAS 
       160        170        180        190        200
PRFTLPVPGY EGYREPLCLS PASSGSSASF ISDTFSPYTS PCVSPNNAGP 
       210        220        230        240        250
DDLCPQFQNI PAHYSPRTSP IMSPRTSLAE DSCLGRHSPV PRPASRSSSP 
       260        270        280        290        300
GAKRRHSCAE ALVAPLPAAS PQRSRSPSPQ PSPHVALQDD SIPAGYPPTA 
       310        320        330        340        350
GSAVLMDALN TLATDSPCGI PSKIWKTSPD PTPVSTAPSK AGLARHIYPT 
       360        370        380        390        400
VEFLGPCEQE ERRNSAPESI LLVPPTWPKQ LVPAIPICSI PVTASLPPLE 
       410        420        430        440        450
WPLSNQSGSY ELRIEVQPKP HHRAHYETEG SRGAVKAPTG GHPVVQLHGY 
       460        470        480        490        500
MENKPLGLQI FIGTADERIL KPHAFYQVHR ITGKTVTTTS YEKIVGNTKV 
       510        520        530        540        550
LEIPLEPKNN MRATIDCAGI LKLRNADIEL RKGETDIGRK NTRVRLVFRV 
       560        570        580        590        600
HVPEPSGRIV SLQAASNPIE CSQRSAHELP MVERQDMDSC LVYGGQQMIL 
       610        620        630        640        650
TGQNFTAESK VVFMEKTTDG QQIWEMEATV DKDKSQPNML FVEIPEYRNK 
       660        670        680        690        700
HIRVPVKVNF YVINGKRKRS QPQHFTYHPV PAIKTEPSDE YEPSLICSPA 
       710        720        730        740        750
HGGLGSQPYY PQHPMLAESP SCLVATMAPC QQFRSGLSSP DARYQQQSPA 
       760        770        780        790        800
AALYQRSKSL SPGLLGYQQP SLLAAPLGLA DAHRSVLVHA GSQGQGQGST 
       810        820        830        840        850
LPHTSSASQQ ASPVIHYSPT NQQLRGGGHQ EFQHIMYCEN FGPSSARPGP 
       860        870        880        890        900
PPINQGQRLS PGAYPTVIQQ QTAPSQRAAK NGPSDQKEAL PTGVTVKQEQ 
       910        920 
NLDQTYLDDV NEIIRKEFSG PPSRNQT

Length:927

Mass (Da):100,020

Last modified:July 27, 2011 - v3

Checksum:ⁱF21E7BABE7DBB40F

GO

Isoform B (identifier: Q60591-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
910-927: VNEIIRKEFSGPPSRNQT → ELIDTHLSWIQNIL

« Hide

        10         20         30         40         50
MDVPEPQPDP DGGDGPGHEP GGSPQDELDF SILFDYDYLN PIEEEPIAHK 
        60         70         80         90        100
AISSPSGLAY PDDVLDYGLK PCNPLASLSG EPPGRFGEPD SIGFQNFLSP 
       110        120        130        140        150
VKPAGASGPS PRIEITPSHE LMQAGGALRG RDAGLSPEQP ALALAGVAAS 
       160        170        180        190        200
PRFTLPVPGY EGYREPLCLS PASSGSSASF ISDTFSPYTS PCVSPNNAGP 
       210        220        230        240        250
DDLCPQFQNI PAHYSPRTSP IMSPRTSLAE DSCLGRHSPV PRPASRSSSP 
       260        270        280        290        300
GAKRRHSCAE ALVAPLPAAS PQRSRSPSPQ PSPHVALQDD SIPAGYPPTA 
       310        320        330        340        350
GSAVLMDALN TLATDSPCGI PSKIWKTSPD PTPVSTAPSK AGLARHIYPT 
       360        370        380        390        400
VEFLGPCEQE ERRNSAPESI LLVPPTWPKQ LVPAIPICSI PVTASLPPLE 
       410        420        430        440        450
WPLSNQSGSY ELRIEVQPKP HHRAHYETEG SRGAVKAPTG GHPVVQLHGY 
       460        470        480        490        500
MENKPLGLQI FIGTADERIL KPHAFYQVHR ITGKTVTTTS YEKIVGNTKV 
       510        520        530        540        550
LEIPLEPKNN MRATIDCAGI LKLRNADIEL RKGETDIGRK NTRVRLVFRV 
       560        570        580        590        600
HVPEPSGRIV SLQAASNPIE CSQRSAHELP MVERQDMDSC LVYGGQQMIL 
       610        620        630        640        650
TGQNFTAESK VVFMEKTTDG QQIWEMEATV DKDKSQPNML FVEIPEYRNK 
       660        670        680        690        700
HIRVPVKVNF YVINGKRKRS QPQHFTYHPV PAIKTEPSDE YEPSLICSPA 
       710        720        730        740        750
HGGLGSQPYY PQHPMLAESP SCLVATMAPC QQFRSGLSSP DARYQQQSPA 
       760        770        780        790        800
AALYQRSKSL SPGLLGYQQP SLLAAPLGLA DAHRSVLVHA GSQGQGQGST 
       810        820        830        840        850
LPHTSSASQQ ASPVIHYSPT NQQLRGGGHQ EFQHIMYCEN FGPSSARPGP 
       860        870        880        890        900
PPINQGQRLS PGAYPTVIQQ QTAPSQRAAK NGPSDQKEAL PTGVTVKQEQ 
       910        920 
NLDQTYLDDE LIDTHLSWIQ NIL

Show »

Length:923

Mass (Da):99,642

Checksum:ⁱA2B4B0476A75A83D

GO

Isoform D (identifier: Q60591-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
619-806: DGQQIWEMEA...QGSTLPHTSS → GPAGTCETRP...PGSSLVLLAL

« Hide

        10         20         30         40         50
MDVPEPQPDP DGGDGPGHEP GGSPQDELDF SILFDYDYLN PIEEEPIAHK 
        60         70         80         90        100
AISSPSGLAY PDDVLDYGLK PCNPLASLSG EPPGRFGEPD SIGFQNFLSP 
       110        120        130        140        150
VKPAGASGPS PRIEITPSHE LMQAGGALRG RDAGLSPEQP ALALAGVAAS 
       160        170        180        190        200
PRFTLPVPGY EGYREPLCLS PASSGSSASF ISDTFSPYTS PCVSPNNAGP 
       210        220        230        240        250
DDLCPQFQNI PAHYSPRTSP IMSPRTSLAE DSCLGRHSPV PRPASRSSSP 
       260        270        280        290        300
GAKRRHSCAE ALVAPLPAAS PQRSRSPSPQ PSPHVALQDD SIPAGYPPTA 
       310        320        330        340        350
GSAVLMDALN TLATDSPCGI PSKIWKTSPD PTPVSTAPSK AGLARHIYPT 
       360        370        380        390        400
VEFLGPCEQE ERRNSAPESI LLVPPTWPKQ LVPAIPICSI PVTASLPPLE 
       410        420        430        440        450
WPLSNQSGSY ELRIEVQPKP HHRAHYETEG SRGAVKAPTG GHPVVQLHGY 
       460        470        480        490        500
MENKPLGLQI FIGTADERIL KPHAFYQVHR ITGKTVTTTS YEKIVGNTKV 
       510        520        530        540        550
LEIPLEPKNN MRATIDCAGI LKLRNADIEL RKGETDIGRK NTRVRLVFRV 
       560        570        580        590        600
HVPEPSGRIV SLQAASNPIE CSQRSAHELP MVERQDMDSC LVYGGQQMIL 
       610        620        630        640        650
TGQNFTAESK VVFMEKTTGP AGTCETRPLP ISLISADRLS PWLSRLQRNP 
       660        670        680        690        700
PGSVFRCSVL LPAPGSSLVL LALASQQASP VIHYSPTNQQ LRGGGHQEFQ 
       710        720        730        740        750
HIMYCENFGP SSARPGPPPI NQGQRLSPGA YPTVIQQQTA PSQRAAKNGP 
       760        770        780        790 
SDQKEALPTG VTVKQEQNLD QTYLDDVNEI IRKEFSGPPS RNQT

Show »

Length:794

Mass (Da):85,181

Checksum:ⁱ1A8246FF98E219B8

GO

Isoform A (identifier: Q60591-1)

Sequence is not available

Note: PubMed:8668213 (AAC52929) sequence is a chimeric cDNA.Curated

Length:–

Mass (Da):–

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A2AQC8	A2AQC8_MOUSE	Nuclear factor of activated T-cells... Nuclear factor of activated T-cells c2 isoform IA-IIL-VIIa (Nuclear factor of-activated T-cells, cytoplasmic 2)	Nfatc2	653	Annotation score:
B5B2R5	B5B2R5_MOUSE	Nuclear factor of activated T-cells... Nuclear factor of activated T-cells c2 isoform IB-IIL-VIIa (Nuclear factor of-activated T-cells, cytoplasmic 2)	Nfatc2	673	Annotation score:
Q8C443	Q8C443_MOUSE	Nuclear factor of activated T-cells... Nuclear factor of activated T-cells c2 isoform IA-IIS-VIIa (Nuclear factor of activated T-cells c2 isoform IB-IIS-VIIa) (Nuclear factor of-activated T-cells, cytoplasmic 2)	Nfatc2	452	Annotation score:

Sequence cautionⁱ

The sequence AAC52929 differs from that shown. Chimeric cDNA fused with Phyhd1 (Lrrc8a).Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	78	L → P in AAK49895 (PubMed:11278367).Curated	1
Sequence conflictⁱ	287	L → P in AAC52929 (PubMed:8668213).Curated	1
Sequence conflictⁱ	287	L → P in AAC52930 (PubMed:8668213).Curated	1
Sequence conflictⁱ	287	L → P in AAC52931 (PubMed:8668213).Curated	1
Sequence conflictⁱ	802	P → R in AAC52929 (PubMed:8668213).Curated	1
Sequence conflictⁱ	802	P → R in AAC52930 (PubMed:8668213).Curated	1
Sequence conflictⁱ	802	P → R in AAC52931 (PubMed:8668213).Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_005597	619 – 806	DGQQI…PHTSS → GPAGTCETRPLPISLISADR LSPWLSRLQRNPPGSVFRCS VLLPAPGSSLVLLAL in isoform D. 1 PublicationAdd BLAST		188
Alternative sequenceⁱVSP_005596	910 – 927	VNEII…SRNQT → ELIDTHLSWIQNIL in isoform B. 1 PublicationAdd BLAST		18

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U02079 mRNA Translation: AAC52929.1 Sequence problems. U36575 mRNA Translation: AAC52930.1 U36576 mRNA Translation: AAC52931.1 AF289078 mRNA Translation: AAK49895.1 EU887588 mRNA Translation: ACG55608.1 AL840639 Genomic DNA No translation available. AL844575 Genomic DNA No translation available.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS17112.1 [Q60591-3] CCDS50803.1 [Q60591-2]
PIRⁱ	A48753
NCBI Reference Sequences More...RefSeqⁱ	NP_035029.2, NM_010899.3 [Q60591-3]

Genome annotation databases

Ensemblⁱ	ENSMUST00000074618; ENSMUSP00000074198; ENSMUSG00000027544 [Q60591-3] ENSMUST00000109184; ENSMUSP00000104812; ENSMUSG00000027544 [Q60591-2]
GeneIDⁱ	18019
KEGGⁱ	mmu:18019
UCSC genome browser More...UCSCⁱ	uc008oau.2 mouse [Q60591-3]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q60591	Nuclear factor of activated T-cells c2 isoform IA-IIS-deltaXa		MOUSE		706	UniRef100_Q60591
Nuclear factor of activated T-cells c2 isoform IA-deltaII-deltaXa		MOUSE		477
Uncharacterized protein (Fragment)		MOUSE		173
Q60591-2	Nuclear factor of activated T-cells c2 isoform IB-IIL-Xa		MOUSE		923	UniRef100_B5B2P7
Nuclear factor of activated T-cells c2 isoform IA-deltaII-Xa		MOUSE		473
Nuclear factor of activated T-cells c2 isoform IA-IIS-Xa		MOUSE		702
Q60591-4	Nuclear factor of activated T-cells c2 isoform IA-IIS-VIIa		MOUSE		452	UniRef100_Q60591-4
Nuclear factor of activated T-cells c2 isoform IA-deltaII-VIIa		MOUSE		223
Nuclear factor of activated T-cells c2 isoform IB-IIL-VIIa		MOUSE		673

Protein	Similar proteins		Species	Score	Length	Source
Q60591	Nuclear factor of activated T-cells c2 isoform IA-IIS-deltaXa		MOUSE		706	UniRef90_Q60591
Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 (Predicted), isoform CRA_b		RAT		931
nuclear factor of activated T-cells, cytoplasmic 2 isoform X1		MESAU		929
UPI0012615846		MASCO		1018
UPI0013F32374		RATRT		933
+34
Q60591-4	Nuclear factor of activated T-cells c2 isoform IA-IIS-VIIa		MOUSE		452	UniRef90_Q60591-4
UPI0013F37A47		RATRT		674
UPI0000DA24A9		RAT		674
UPI000A3054DA		MUSPA		673
UPI000A317CA9		MUSCR		673
+8

Protein	Similar proteins		Species	Score	Length	Source
Q60591	Nuclear factor of activated T-cells, cytoplasmic 2	)	HUMAN		925	UniRef50_Q60591
Nuclear factor of activated T-cells c2 isoform IA-IIS-deltaXa		MOUSE		706
Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 (Predicted), isoform CRA_b		RAT		931
nuclear factor of activated T-cells, cytoplasmic 2 isoform X1		MESAU		929
Uncharacterized protein		PODMU		921
+418
Q60591-4	Nuclear factor of activated T-cells c2 isoform IA-IIS-VIIa		MOUSE		452	UniRef50_Q60591-4
Nuclear factor of activated T cells 2		FICAL		692
RHD domain-containing protein		PANPA		719
Nuclear factor of activated T cells 2		LYNCA		695
Nuclear factor of activated T cells 2		NOMLE		706
+27

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U02079 mRNA Translation: AAC52929.1 Sequence problems. U36575 mRNA Translation: AAC52930.1 U36576 mRNA Translation: AAC52931.1 AF289078 mRNA Translation: AAK49895.1 EU887588 mRNA Translation: ACG55608.1 AL840639 Genomic DNA No translation available. AL844575 Genomic DNA No translation available.
CCDSⁱ	CCDS17112.1 [Q60591-3] CCDS50803.1 [Q60591-2]
PIRⁱ	A48753
RefSeqⁱ	NP_035029.2, NM_010899.3 [Q60591-3]

3D structure databases

SMRⁱ	Q60591
ModBaseⁱ	Search...

Protein-protein interaction databases

BioGRIDⁱ	201739, 2 interactors
ComplexPortalⁱ	CPX-610 AP-1 transcription factor complex FOS-JUN-NFATC2
CORUMⁱ	Q60591
DIPⁱ	DIP-49476N
ELMⁱ	Q60591
IntActⁱ	Q60591, 11 interactors
STRINGⁱ	10090.ENSMUSP00000074198

Protein family/group databases

MoonDBⁱ	Q60591 Predicted

MoonDBⁱ

Q60591 Predicted

PTM databases

iPTMnetⁱ	Q60591
PhosphoSitePlusⁱ	Q60591

Proteomic databases

EPDⁱ	Q60591
jPOSTⁱ	Q60591
PaxDbⁱ	Q60591
PeptideAtlasⁱ	Q60591
PRIDEⁱ	Q60591

Protocols and materials databases

Antibodypediaⁱ	1758 508 antibodies

Antibodypediaⁱ

1758 508 antibodies

Genome annotation databases

Ensemblⁱ	ENSMUST00000074618; ENSMUSP00000074198; ENSMUSG00000027544 [Q60591-3] ENSMUST00000109184; ENSMUSP00000104812; ENSMUSG00000027544 [Q60591-2]
GeneIDⁱ	18019
KEGGⁱ	mmu:18019
UCSCⁱ	uc008oau.2 mouse [Q60591-3]

Organism-specific databases

CTDⁱ	4773
MGIⁱ	MGI:102463 Nfatc2

Phylogenomic databases

eggNOGⁱ	ENOG410IHFR Eukaryota ENOG41105U2 LUCA
GeneTreeⁱ	ENSGT00940000156230
HOGENOMⁱ	CLU_010185_1_0_1
InParanoidⁱ	Q60591
KOⁱ	K17332
OMAⁱ	CENFPPG
TreeFamⁱ	TF326480

Enzyme and pathway databases

Reactomeⁱ	R-MMU-2025928 Calcineurin activates NFAT R-MMU-2871809 FCERI mediated Ca+2 mobilization R-MMU-5607763 CLEC7A (Dectin-1) induces NFAT activation

Reactomeⁱ

R-MMU-2025928 Calcineurin activates NFAT
R-MMU-2871809 FCERI mediated Ca+2 mobilization
R-MMU-5607763 CLEC7A (Dectin-1) induces NFAT activation

Miscellaneous databases

BioGRID-ORCSⁱ	18019 4 hits in 12 CRISPR screens
ChiTaRSⁱ	Nfatc2 mouse
Protein Ontology More...PROⁱ	PR:Q60591
RNActⁱ	Q60591 protein
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000027544 Expressed in lumbar subsegment of spinal cord and 228 other tissues
ExpressionAtlasⁱ	Q60591 baseline and differential
Genevisibleⁱ	Q60591 MM

Family and domain databases

Gene3Dⁱ	2.60.40.10, 1 hit 2.60.40.340, 1 hit
InterProⁱ	View protein in InterPro IPR013783 Ig-like_fold IPR014756 Ig_E-set IPR002909 IPT_dom IPR008366 NFAT IPR008967 p53-like_TF_DNA-bd IPR032397 RHD_dimer IPR011539 RHD_DNA_bind_dom IPR037059 RHD_DNA_bind_dom_sf
PANTHERⁱ	PTHR12533 PTHR12533, 1 hit
Pfamⁱ	View protein in Pfam PF16179 RHD_dimer, 1 hit PF00554 RHD_DNA_bind, 1 hit
PRINTSⁱ	PR01789 NUCFACTORATC
SMARTⁱ	View protein in SMART SM00429 IPT, 1 hit
SUPFAMⁱ	SSF49417 SSF49417, 1 hit SSF81296 SSF81296, 1 hit
PROSITEⁱ	View protein in PROSITE PS50254 REL_2, 1 hit
ProtoNetⁱ	Search...
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NFAC2_MOUSE
Accessionⁱ	Q60591Primary (citable) accession number: Q60591 Secondary accession number(s): A2APK2 Q91Y65
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
	Last sequence update:	July 27, 2011
	Last modified:	June 17, 2020
	This is version 193 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Direct protein sequencing, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

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Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q60591 (NFAC2_MOUSE)

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Nuclear factor of activated T-cells, cytoplasmic 2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Cytosol

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Q60591

GO - Molecular functioni

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Motif

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ