Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per monomer.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (hemB)
  2. Probable porphobilinogen deaminase (hemC)
  3. Putative uroporphyrinogen-III synthase (hemD)
  4. no protein annotated in this organism
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi129Zinc; catalyticBy similarity1
Metal bindingi131Zinc; catalyticBy similarity1
Metal bindingi139Zinc; catalyticBy similarity1
Active sitei204Schiff-base intermediate with substrateBy similarity1
Binding sitei214Substrate 1By similarity1
Binding sitei226Substrate 1By similarity1
Metal bindingi242MagnesiumBy similarity1
Active sitei257Schiff-base intermediate with substrateBy similarity1
Binding sitei283Substrate 2By similarity1
Binding sitei322Substrate 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processHeme biosynthesis, Porphyrin biosynthesis
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALAD
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:hemB
Ordered Locus Names:MJ0643
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001405231 – 335Delta-aminolevulinic acid dehydrataseAdd BLAST335

Proteomic databases

PRIDEiQ60178

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi243232.MJ_0643

Structurei

3D structure databases

ProteinModelPortaliQ60178
SMRiQ60178
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Phylogenomic databases

eggNOGiarCOG04300 Archaea
COG0113 LUCA
InParanoidiQ60178
KOiK01698
OMAiYQMDYAN
OrthoDBiPOG093Z05G9
PhylomeDBiQ60178

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR001731 ALAD
IPR030656 ALAD_AS
IPR013785 Aldolase_TIM
PANTHERiPTHR11458 PTHR11458, 1 hit
PfamiView protein in Pfam
PF00490 ALAD, 1 hit
PIRSFiPIRSF001415 Porphbilin_synth, 1 hit
PRINTSiPR00144 DALDHYDRTASE
SMARTiView protein in SMART
SM01004 ALAD, 1 hit
PROSITEiView protein in PROSITE
PS00169 D_ALA_DEHYDRATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q60178-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKINLGDFM LIRPRRLRKN QKIRDLVRET ILTKNDLIMP IFVDENLKGN
60 70 80 90 100
EKKEISSMPN QYRFSVEGAI EEAKEIADLG IPAVILFGIP KHKDEIASSA
110 120 130 140 150
YDKNGVVQRT IRGIKEELGD ELLVIADCCL CEYTSHGHCG IVKDGKILND
160 170 180 190 200
ATLPILAKIA LSYADAGVDI VAPSDMMDGR VRAIREILEE NGYDDVAIMS
210 220 230 240 250
YSAKYASSFY GPFREAAESA PKFGDRKSYQ MDIGNAREAL KEIALDIEEG
260 270 280 290 300
ADLILVKPAL PYLDIIRMAK DRFDVPIGGY CVSGEYAMVE AAARNGWLDR
310 320 330
EKVIYEILLS IKRAGADFII TYWAKEVAEI GLSQE
Length:335
Mass (Da):37,396
Last modified:November 1, 1997 - v1
Checksum:i4D097DAB45F3705D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA Translation: AAB98637.1
PIRiC64380

Genome annotation databases

EnsemblBacteriaiAAB98637; AAB98637; MJ_0643
KEGGimja:MJ_0643

Similar proteinsi

Entry informationi

Entry nameiHEM2_METJA
AccessioniPrimary (citable) accession number: Q60178
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 7, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health