UniProtKB - Q5ZYS8 (Q5ZYS8_LEGPH)
Protein
Formate dehydrogenase
Gene
lpg0283
Organism
Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
Status
Functioni
Catalyzes the NAD+-dependent oxidation of formate to carbon dioxide. Formate oxidation is the final step in the methanol oxidation pathway in methylotrophic microorganisms. Has a role in the detoxification of exogenous formate in non-methylotrophic organisms.UniRule annotation
Catalytic activityi
- EC:1.17.1.9UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 128 | Substrate; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 152 | SubstrateUniRule annotation | 1 | |
Binding sitei | 153 | NADUniRule annotation | 1 | |
Binding sitei | 227 | NADUniRule annotation | 1 | |
Binding sitei | 288 | NAD; via carbonyl oxygenUniRule annotation | 1 | |
Sitei | 290 | Important for catalytic activityUniRule annotation | 1 | |
Binding sitei | 314 | NADUniRule annotation | 1 | |
Sitei | 338 | Important for catalytic activityUniRule annotation | 1 | |
Binding sitei | 386 | NADUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 207 – 208 | NADUniRule annotation | 2 | |
Nucleotide bindingi | 262 – 266 | NADUniRule annotation | 5 | |
Nucleotide bindingi | 338 – 341 | NADUniRule annotation | 4 |
GO - Molecular functioni
- formate dehydrogenase (NAD+) activity Source: UniProtKB-UniRule
- NAD binding Source: InterPro
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
GO - Biological processi
- formate catabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | OxidoreductaseUniRule annotationImported |
Ligand | NADUniRule annotation |
Names & Taxonomyi
Protein namesi | Recommended name: Formate dehydrogenaseUniRule annotation (EC:1.17.1.9UniRule annotation)Short name: FDHUniRule annotation Alternative name(s): NAD-dependent formate dehydrogenaseUniRule annotation |
Gene namesi | Ordered Locus Names:lpg0283Imported |
Organismi | Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)Imported |
Taxonomic identifieri | 272624 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmUniRule annotationInteractioni
Subunit structurei
Homodimer.UniRule annotation
Protein-protein interaction databases
STRINGi | 272624.lpg0283 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 67 – 366 | 2-Hacid_dhInterPro annotationAdd BLAST | 300 | |
Domaini | 161 – 339 | 2-Hacid_dh_CInterPro annotationAdd BLAST | 179 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 8 – 152 | CatalyticUniRule annotationAdd BLAST | 145 | |
Regioni | 153 – 339 | Coenzyme-bindingUniRule annotationAdd BLAST | 187 | |
Regioni | 340 – 387 | CatalyticUniRule annotationAdd BLAST | 48 |
Sequence similaritiesi
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105FDJ Bacteria COG1052 LUCA |
HOGENOMi | HOG000136703 |
KOi | K00122 |
OMAi | EVTYCNS |
Family and domain databases
CDDi | cd05302 FDH, 1 hit |
HAMAPi | MF_03210 Formate_dehydrogenase, 1 hit |
InterProi | View protein in InterPro IPR006139 D-isomer_2_OHA_DH_cat_dom IPR029753 D-isomer_DH_CS IPR006140 D-isomer_DH_NAD-bd IPR033689 FDH_NAD-dep IPR036291 NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389 2-Hacid_dh, 1 hit PF02826 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735 SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00670 D_2_HYDROXYACID_DH_2, 1 hit PS00671 D_2_HYDROXYACID_DH_3, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5ZYS8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFSQSQQKIV CVLYDDPKGG FPPNYARESI PELKQYPDGQ SLPNPDSIDF
60 70 80 90 100
IPGEMLGSVS GELGLRQFLE SNGHQLVVTS DKDGSDSVFA RELKEATVVI
110 120 130 140 150
SQPFWPAYLT RDRIESAPKL KLAITAGIGS DHVDLQAAME HNITVCEVTY
160 170 180 190 200
CNSISVAEHT VMMILALVRD FIPQYNTVID GGWNIADCVS RSYDLEGMQV
210 220 230 240 250
GCVAAGRIGL AVLRRLKPFA VKLHYTDRHR LPVQLEQELN LTYHPSVESM
260 270 280 290 300
VKVCDVVSIH CPLHPETEYL FDERLIKQMK RGSYLINTAR GRICDQHAVA
310 320 330 340 350
DALESGHLAG YAGDVWFPQP PAKNHPWRSM PNHAMTPHTS GTTLSAQARY
360 370 380 390 400
AAGVREILEC WLGNKPIREE YLIVSQGHLA GVGSHSYSAG NTTSGTEQAA
ELV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017354 Genomic DNA Translation: AAU26390.1 |
RefSeqi | WP_010946044.1, NC_002942.5 YP_094337.1, NC_002942.5 |
Genome annotation databases
EnsemblBacteriai | AAU26390; AAU26390; lpg0283 |
GeneIDi | 19831850 |
KEGGi | lpn:lpg0283 |
PATRICi | fig|272624.6.peg.299 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017354 Genomic DNA Translation: AAU26390.1 |
RefSeqi | WP_010946044.1, NC_002942.5 YP_094337.1, NC_002942.5 |
3D structure databases
ProteinModelPortali | Q5ZYS8 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
STRINGi | 272624.lpg0283 |
Proteomic databases
PaxDbi | Q5ZYS8 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
EnsemblBacteriai | AAU26390; AAU26390; lpg0283 |
GeneIDi | 19831850 |
KEGGi | lpn:lpg0283 |
PATRICi | fig|272624.6.peg.299 |
Phylogenomic databases
eggNOGi | ENOG4105FDJ Bacteria COG1052 LUCA |
HOGENOMi | HOG000136703 |
KOi | K00122 |
OMAi | EVTYCNS |
Family and domain databases
CDDi | cd05302 FDH, 1 hit |
HAMAPi | MF_03210 Formate_dehydrogenase, 1 hit |
InterProi | View protein in InterPro IPR006139 D-isomer_2_OHA_DH_cat_dom IPR029753 D-isomer_DH_CS IPR006140 D-isomer_DH_NAD-bd IPR033689 FDH_NAD-dep IPR036291 NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389 2-Hacid_dh, 1 hit PF02826 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735 SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00670 D_2_HYDROXYACID_DH_2, 1 hit PS00671 D_2_HYDROXYACID_DH_3, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | Q5ZYS8_LEGPH | |
Accessioni | Q5ZYS8Primary (citable) accession number: Q5ZYS8 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | November 23, 2004 |
Last sequence update: | November 23, 2004 | |
Last modified: | December 5, 2018 | |
This is version 89 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |