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Entry version 63 (02 Dec 2020)
Sequence version 1 (23 Nov 2004)
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Protein

Subversion of eukaryotic traffic protein A

Gene

setA

Organism
Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Secreted effector that interferes with vesicular trafficking of host cells. Possesses glucohydrolase and mono-O-glucosyltransferase activity by using UDP-glucose as a sugar donor substrate. Is able to glucosylate histones H4 and H3.1 in vitro, but it is unlikely that histones are the natural substrates for SetA. May glycosylate a component of the host cell vesicle trafficking machinery during L.pneumophila infection. Binds with high specificity to phosphatidylinositol 3-phosphate (PtdIns3P), (with a dissociation constant value of 809 nM), which guides SetA to the cytosolic leaflet of the early phagosome of the host cell.2 Publications

Miscellaneous

Production of SetA in yeast is extremely toxic, it causes a severe growth defect and alters yeast cell morphology. When expressed in mammalian cells, SetA causes cell retractation.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processVirulence

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Subversion of eukaryotic traffic protein A
Alternative name(s):
Effector protein SetA
Subversion of eukaryotic vesicle trafficking A
Including the following 2 domains:
Glucosyltransferase (EC:2.4.1.-)
Phosphatidylinositol-3-phosphate-binding domain
Alternative name(s):
PtdIns(3)P-binding domain
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:setA
Ordered Locus Names:lpg1978
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiLegionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri272624 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesLegionellaceaeLegionella
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000609 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

  • Secreted 2 Publications
  • Note: Is secreted by the Dot/Icm type IV secretion system (T4SS) into the host cell cytosol. Localizes to vesicular compartments in mammalian cells, more precisely to early endosomes and early Legionella-containing phagosomes. After translocation by the Dot/Icm system, a portion of SetA associates with host cell membranes while another portion of SetA remains in the host cell cytosol.

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Cells lacking this gene grow similarly to wild-type L.pneumophila in both murine bone marrow-derived macrophages and the soil amoeba Dictyostelium discoideum, indicating that SetA is not required for efficient intracellular replication of L.pneumophila in host cells under laboratory growth conditions.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi134 – 136DSD → ASA: Alleviates the toxicity of SetA to host cell growth, and blocks vesicular sorting defects. 2 Publications3
Mutagenesisi134 – 136DSD → NSN: Abolishes glucohydrolase and glucosyltransferase activities. Fails to induce any cellular alterations when expressed in mammalian cells. 2 Publications3

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004224231 – 644Subversion of eukaryotic traffic protein AAdd BLAST644

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Ubiquitinated and polyubiquitinated when ectopically produced in both yeast and mammalian cells; however it is unsure if this modification occurs during the L.pneumophila infection of host cells.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q5ZU30

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Is induced in the post-exponential phase of growth.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
272624.lpg1978

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 400GlucosyltransferaseAdd BLAST400
Regioni401 – 644PtdIns(3)P-binding and localization domainAdd BLAST244

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Multidomain protein with an N-terminal glucosyltransferase domain and a C-terminal phosphatidylinositol 3-phosphate-binding domain, which guides the Legionella effector to the surface of the Legionella-containing vacuole. Both domains are essential for the cellular effects on the eukaryotic host.1 Publication

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG3774, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_433330_0_0_6

Identification of Orthologs from Complete Genome Data

More...
OMAi
SEMEAIH

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR007577, GlycoTrfase_DXD_sugar-bd_CS
IPR029044, Nucleotide-diphossugar_trans

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF04488, Gly_transf_sug, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF53448, SSF53448, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q5ZU30-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MYKIYSYLGW RIDMKTENLP QAGQEAQIDK KIHFIWVGHI MPQKNIQVVS
60 70 80 90 100
EWAEKNPGYE TIIWVDKKIA PAKELDLFIL DMKSKGITVK DINEEGVCRD
110 120 130 140 150
SIRHELDQES PNYGMVSDML RLNILAAEGG IYLDSDILCS APFPDEIYAP
160 170 180 190 200
FGFLLSPWSQ GANNTLCNDI ILCSKGNQII QQLADAIEQS YIARDSFEFT
210 220 230 240 250
HEYASMKETK GERIAKTLGV TGPGFLFHQL KKMGILNDKS EMEAIHWELQ
260 270 280 290 300
DQRYLIDGSV KEPDYFYVPQ NNTNDASWVP SIKRPGIENM SFQERLENAV
310 320 330 340 350
QLIAFDIQKT GLFNLDHYAN ELKVKQNSWC IAAETSPELK PDSYLLIRPR
360 370 380 390 400
DKTGEWTLYY VDEDKKLNPV TLPVIKGAIK LSEVSDPLRK FHTLLSQVSD
410 420 430 440 450
PVNPTAHELK QIGRALIELK PRQDEWHCKN KWSGAEEIAQ ELWQRITSNE
460 470 480 490 500
TLRAQIKQCF TQFESLKPRV AELGLTRASG AGTEVEAHES TVKEQEIISQ
510 520 530 540 550
NTVGEEGTKE KNSVQLASEN SSDEKIKTAH DLIDEIIQDV IQLDGKLGLL
560 570 580 590 600
GGNTRQLEDG RVINIPNGAA MIFDDYKKYK QGELTAESAL ESMIKIAKLS
610 620 630 640
NQLNRHTFFN QRQPETGQFY KKVAAIDLQT TIAAEYDNNH GLRI
Length:644
Mass (Da):73,129
Last modified:November 23, 2004 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i7D87DA7DB80EC013
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AE017354 Genomic DNA Translation: AAU28047.1

NCBI Reference Sequences

More...
RefSeqi
WP_010947694.1, NC_002942.5
YP_095994.1, NC_002942.5

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAU28047; AAU28047; lpg1978

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
lpn:lpg1978

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|272624.6.peg.2071

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017354 Genomic DNA Translation: AAU28047.1
RefSeqiWP_010947694.1, NC_002942.5
YP_095994.1, NC_002942.5

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

STRINGi272624.lpg1978

Proteomic databases

PaxDbiQ5ZU30

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
3078498

Genome annotation databases

EnsemblBacteriaiAAU28047; AAU28047; lpg1978
KEGGilpn:lpg1978
PATRICifig|272624.6.peg.2071

Phylogenomic databases

eggNOGiCOG3774, Bacteria
HOGENOMiCLU_433330_0_0_6
OMAiSEMEAIH

Family and domain databases

InterProiView protein in InterPro
IPR007577, GlycoTrfase_DXD_sugar-bd_CS
IPR029044, Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF04488, Gly_transf_sug, 1 hit
SUPFAMiSSF53448, SSF53448, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSETA_LEGPH
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q5ZU30
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: November 23, 2004
Last modified: December 2, 2020
This is version 63 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome
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