UniProtKB - Q5ZMS3 (IF2G_CHICK)
Protein
Eukaryotic translation initiation factor 2 subunit 3
Gene
EIF2S3
Organism
Gallus gallus (Chicken)
Status
Functioni
As a subunit of eukaryotic initiation factor 2 (eIF-2), involved in the early steps of protein synthesis. In the presence of GTP, eIF-2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex and initiation factors eIF-1, eIF-1A and eIF-3 to form the 43S pre-initiation complex (43S PIC), a step that determines the rate of protein translation. The 43S PIC binds to mRNA and scans downstream to the initiation codon, where it forms a 48S initiation complex by codon-anticodon base pairing. This leads to the displacement of eIF-1 to allow GTPase-activating protein (GAP) eIF-5-mediated hydrolysis of eIF2-bound GTP. Hydrolysis of GTP and release of Pi, which makes GTP hydrolysis irreversible, causes the release of the eIF-2-GDP binary complex from the 40S subunit, an event that is essential for the subsequent joining of the 60S ribosomal subunit to form an elongation-competent 80S ribosome. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must be exchanged with GTP by way of a reaction catalyzed by GDP-GTP exchange factor (GEF) eIF-2B.By similarity
Catalytic activityi
- EC:3.6.5.3By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 51 – 56 | GTPBy similarity | 6 | |
Nucleotide bindingi | 190 – 193 | GTPBy similarity | 4 | |
Nucleotide bindingi | 225 – 227 | GTPBy similarity | 3 |
GO - Molecular functioni
- GTPase activity Source: InterPro
- GTP binding Source: UniProtKB-KW
- translation initiation factor activity Source: GO_Central
- tRNA binding Source: InterPro
GO - Biological processi
- formation of translation preinitiation complex Source: GO_Central
- positive regulation of translational fidelity Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Initiation factor |
Biological process | Protein biosynthesis |
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic translation initiation factor 2 subunit 3 (EC:3.6.5.3)Alternative name(s): Eukaryotic translation initiation factor 2 subunit gamma Short name: eIF-2-gamma |
Gene namesi | Name:EIF2S3 ORF Names:RCJMB04_1f8 |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- eukaryotic translation initiation factor 2 complex Source: UniProtKB
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000280446 | 2 – 472 | Eukaryotic translation initiation factor 2 subunit 3Add BLAST | 471 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanine; partial1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | Q5ZMS3 |
Interactioni
Subunit structurei
The eukaryotic translation initiation factor 2 complex/eIF2 is a heterotrimer composed of an alpha, a beta and a gamma subunit.
By similarityProtein-protein interaction databases
BioGRIDi | 679807, 1 interactor |
STRINGi | 9031.ENSGALP00000026307 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 247 | tr-type GPROSITE-ProRule annotationAdd BLAST | 209 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 48 – 55 | G1PROSITE-ProRule annotation | 8 | |
Regioni | 76 – 80 | G2PROSITE-ProRule annotation | 5 | |
Regioni | 134 – 137 | G3PROSITE-ProRule annotation | 4 | |
Regioni | 190 – 193 | G4PROSITE-ProRule annotation | 4 | |
Regioni | 225 – 227 | G5PROSITE-ProRule annotation | 3 |
Sequence similaritiesi
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0466, Eukaryota |
InParanoidi | Q5ZMS3 |
OrthoDBi | 903135at2759 |
PhylomeDBi | Q5ZMS3 |
Family and domain databases
CDDi | cd01888, eIF2_gamma, 1 hit cd03688, eIF2_gamma_II, 1 hit cd15490, eIF2_gamma_III, 1 hit |
InterProi | View protein in InterPro IPR004161, EFTu-like_2 IPR015256, eIF2g_C IPR044127, eIF2g_dom_2 IPR044128, eIF2g_GTP-bd IPR027417, P-loop_NTPase IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C |
Pfami | View protein in Pfam PF09173, eIF2_C, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51722, G_TR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q5ZMS3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGDEAGVTL GQPHLSRQDL ATLDVTKLTP LSPEVISRQA TINIGTIGHV
60 70 80 90 100
AHGKSTVVKA ISGVHTVRFK NELERNITIK LGYANAKIYK LDDPSCSRPE
110 120 130 140 150
CYRSCGSSTP DEFPTDIPGT KGNFKLVRHV SFVDCPGHDI LMATMLNGAA
160 170 180 190 200
VMDAALLLIA GNESCPQPQT SEHLAAIEIM KLKHILILQN KIDLVKESQA
210 220 230 240 250
KEQYEQILAF VQGTVAEGAP IIPISAQLKY SIEVVCEYIV KKIPVPLRDF
260 270 280 290 300
TSEPRLIVIR SFDVNKPGCE VDDLKGGVAG GSILKGVLKV GQEIEVRPGI
310 320 330 340 350
VSKDSEGKLM CKPIFSKIVS LFAEHNDLQY AAPGGLIGVG TKIDPTLCRA
360 370 380 390 400
DRMVGQVLGA VGALPEIFTE LEISYFLLRR LLGVRTEGDK KAAKVQKLSK
410 420 430 440 450
NEVLMVNIGS LSTGGRVSAV KADLGKIVLT NPVCTEVGEK IALSRRVEKH
460 470
WRLIGWGQIR RGVTIKPTVD DD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ719311 mRNA Translation: CAG30970.1 |
RefSeqi | NP_001006260.1, NM_001006260.2 |
Genome annotation databases
GeneIDi | 418597 |
KEGGi | gga:418597 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ719311 mRNA Translation: CAG30970.1 |
RefSeqi | NP_001006260.1, NM_001006260.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 679807, 1 interactor |
STRINGi | 9031.ENSGALP00000026307 |
Proteomic databases
PaxDbi | Q5ZMS3 |
Genome annotation databases
GeneIDi | 418597 |
KEGGi | gga:418597 |
Organism-specific databases
CTDi | 1968 |
Phylogenomic databases
eggNOGi | KOG0466, Eukaryota |
InParanoidi | Q5ZMS3 |
OrthoDBi | 903135at2759 |
PhylomeDBi | Q5ZMS3 |
Miscellaneous databases
PROi | PR:Q5ZMS3 |
Family and domain databases
CDDi | cd01888, eIF2_gamma, 1 hit cd03688, eIF2_gamma_II, 1 hit cd15490, eIF2_gamma_III, 1 hit |
InterProi | View protein in InterPro IPR004161, EFTu-like_2 IPR015256, eIF2g_C IPR044127, eIF2g_dom_2 IPR044128, eIF2g_GTP-bd IPR027417, P-loop_NTPase IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C |
Pfami | View protein in Pfam PF09173, eIF2_C, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51722, G_TR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IF2G_CHICK | |
Accessioni | Q5ZMS3Primary (citable) accession number: Q5ZMS3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 2007 |
Last sequence update: | November 23, 2004 | |
Last modified: | February 10, 2021 | |
This is version 101 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Translation initiation factors
List of translation initiation factor entries - SIMILARITY comments
Index of protein domains and families