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Protein

DNA replication licensing factor MCM3

Gene

MCM3

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi346 – 353ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chromatin binding Source: AgBase
  • DNA binding Source: UniProtKB-KW
  • helicase activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM3 (EC:3.6.4.12)
Gene namesi
Name:MCM3
ORF Names:RCJMB04_1j4
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003189041 – 812DNA replication licensing factor MCM3Add BLAST812

Proteomic databases

PaxDbiQ5ZMN2
PRIDEiQ5ZMN2

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Associated with the replication-specific DNA polymerase alpha (By similarity).By similarity

Protein-protein interaction databases

BioGridi682782, 1 interactor
STRINGi9031.ENSGALP00000026858

Structurei

3D structure databases

ProteinModelPortaliQ5ZMN2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini296 – 503MCMAdd BLAST208

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi478 – 481Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

eggNOGiKOG0479 Eukaryota
COG1241 LUCA
HOGENOMiHOG000224126
HOVERGENiHBG104962
InParanoidiQ5ZMN2
KOiK02541
PhylomeDBiQ5ZMN2

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR031327 MCM
IPR008046 Mcm3
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01659 MCMPROTEIN3
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q5ZMN2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPAGGLGD AELREAQRDY LDFLDDEEDQ GVYHGKVRDM ISDNQYRLLV
60 70 80 90 100
NINDLRRRNE KRANRLLSNA FEELIAFQRA LKDFVASVDA TYAKQYEDFY
110 120 130 140 150
IGLEGSFGSK HVSPRTLTAC FLSCIVCVEG IVTKCSLIRP KVVRSVHYCP
160 170 180 190 200
ATKKTIERRY TDLTSLDAFP SSTVYPTKDE ENNPLETEYG LSVYKDHQTI
210 220 230 240 250
SIQEMPEKAP AGQLPRSVDV ILDDDLVDKV KPGDRIQVVG TYRCLPGKKG
260 270 280 290 300
GYTSGTFRTI LIACHIKQMS KDARPLYSAN DVAKIKRFSK SRSKDIFNQL
310 320 330 340 350
ARSLAPSIHG HEFIKKALLC MLLGGVEKVL ENGSRIRGDI NILLIGDPSV
360 370 380 390 400
AKSQLLRYVL GTAPRAVGTT GRGSSGVGLT AAVTTDQETG ERRLEAGAMV
410 420 430 440 450
LADRGVVCID EFDKMSDIDR TAIHEVMEQG RVTIAKAGIH ARLNSRCSVL
460 470 480 490 500
AAANPVYGRY DQYKTPMENI GLQDSLLSRF DLLFIMLDQM DSEQDREISD
510 520 530 540 550
HVLRMHRYRN PNEQDGDAMP LGSAVEILAT DDPDFAQEEE QELQVYEKHD
560 570 580 590 600
DLLHGPNRRK EKIVSMEFMR KYIHVAKMIK PVLTQESADY IAEEYSSLRS
610 620 630 640 650
QNQMNSDIAR TSPVTARTLE TLIRLSTAHA KARMSKTVDL QDAEAALELV
660 670 680 690 700
QFAYFKKVLE KEKKRKKQVE DDSETEKEEE EEETQPEKEG RKQRRKKART
710 720 730 740 750
EGEEESYDPY DFSDAEQEMP EVQAHTPKTP ESSATGEAKK PELADPRLKA
760 770 780 790 800
FKAALLEVFK SSHAQSVGLK NVMESINRDN PEPFSLAGVK VALAHMQDDN
810
QIMVSDDIIF LI
Length:812
Mass (Da):91,251
Last modified:November 23, 2004 - v1
Checksum:i5D65A2F86936E234
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719352 mRNA Translation: CAG31011.1
RefSeqiNP_001006421.1, NM_001006421.1
UniGeneiGga.4920

Genome annotation databases

GeneIDi422043
KEGGigga:422043

Similar proteinsi

Entry informationi

Entry nameiMCM3_CHICK
AccessioniPrimary (citable) accession number: Q5ZMN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 23, 2004
Last modified: December 20, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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