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Protein

Importin subunit alpha-5

Gene

KPNA1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-GGA-211227 Activation of DNA fragmentation factor

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit alpha-5
Alternative name(s):
Karyopherin subunit alpha-1
Gene namesi
Name:KPNA1
ORF Names:RCJMB04_1l14
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002975291 – 538Importin subunit alpha-5Add BLAST538

Proteomic databases

PaxDbiQ5ZML1

Expressioni

Gene expression databases

BgeeiENSGALG00000014426 Expressed in 11 organ(s), highest expression level in skeletal muscle tissue

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000034376

Structurei

3D structure databases

ProteinModelPortaliQ5ZML1
SMRiQ5ZML1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 57IBBPROSITE-ProRule annotationAdd BLAST57
Repeati77 – 117ARM 1; truncatedAdd BLAST41
Repeati118 – 161ARM 2Add BLAST44
Repeati162 – 206ARM 3Add BLAST45
Repeati207 – 245ARM 4Add BLAST39
Repeati246 – 290ARM 5Add BLAST45
Repeati291 – 330ARM 6Add BLAST40
Repeati331 – 372ARM 7Add BLAST42
Repeati373 – 412ARM 8Add BLAST40
Repeati413 – 457ARM 9Add BLAST45
Repeati460 – 504ARM 10; atypicalAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni149 – 241NLS binding site (major)By similarityAdd BLAST93
Regioni245 – 437Binding to RAG1Add BLAST193
Regioni318 – 406NLS binding site (minor)By similarityAdd BLAST89

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 51Nuclear localization signalBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi25 – 28Poly-Arg4

Domaini

Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins (By similarity).By similarity
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding (By similarity).By similarity

Sequence similaritiesi

Belongs to the importin alpha family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0166 Eukaryota
COG5064 LUCA
GeneTreeiENSGT00760000119094
HOGENOMiHOG000167616
HOVERGENiHBG001846
InParanoidiQ5ZML1
KOiK15042
OMAiSWLLFVN
OrthoDBiEOG091G095Z
PhylomeDBiQ5ZML1
TreeFamiTF354205

Family and domain databases

Gene3Di1.20.5.690, 1 hit
1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR032413 Arm_3
IPR000225 Armadillo
IPR002652 Importin-a_IBB
IPR036975 Importin-a_IBB_sf
IPR024931 Importing_su_alpha
PfamiView protein in Pfam
PF00514 Arm, 8 hits
PF16186 Arm_3, 1 hit
PF01749 IBB, 1 hit
PIRSFiPIRSF005673 Importin_alpha, 1 hit
SMARTiView protein in SMART
SM00185 ARM, 8 hits
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50176 ARM_REPEAT, 4 hits
PS51214 IBB, 1 hit

Sequencei

Sequence statusi: Complete.

Q5ZML1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTTSGKENFR LKSYKNKSLN PDEMRRRREE EGLQLRKQKR EEQLFKRRNV
60 70 80 90 100
ATAEEEAEEE VMSDGGFHEA QMNNMEMTSS AVITSDMIEM IFSNSPEQQL
110 120 130 140 150
SATQKFRKLL SKEPNPPIDE VISTPGVVAR FVEFLKRKEN CTLQFEAAWV
160 170 180 190 200
LTNIASGNSL QTRIVIQAGA VPIFIELLSS EFEDVQEQAV WALGNIAGDS
210 220 230 240 250
TMCRDYVLDC NILPPLLQLL SKQNRITMTR NAVWALSNLC RGKNPPPDFA
260 270 280 290 300
KVSPCLSVLS WLLFVNDTDV LADACWALSY LSDGPNDKIQ AVIDAGVCRR
310 320 330 340 350
LVELLMHNDY KVVSPALRAV GNIVTGDDIQ TQVILNCSAL QSLLHLLSSP
360 370 380 390 400
KESIKKEACW TISNITAGNR AQIQTVIDAH IFPALINILQ TAEFRTRKEA
410 420 430 440 450
AWAITNATSG GSAEQIKYLV ELGCIKPLCD LLTVMDSKIV QVALSGLENI
460 470 480 490 500
LRLGEQESKR SGAGINPYCA LIEEAYGLDK IEFLQSHENQ EIYQKAFDLI
510 520 530
EHYFGTEDED SSIAPQVDLS QQQYIFQQCE APMEGFQL
Length:538
Mass (Da):60,195
Last modified:November 23, 2004 - v1
Checksum:i2596F89BC5D23057
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719373 mRNA Translation: CAG31032.1
RefSeqiNP_001025945.1, NM_001030774.1
UniGeneiGga.21666

Genome annotation databases

EnsembliENSGALT00000035021; ENSGALP00000034376; ENSGALG00000014426
GeneIDi418271
KEGGigga:418271

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719373 mRNA Translation: CAG31032.1
RefSeqiNP_001025945.1, NM_001030774.1
UniGeneiGga.21666

3D structure databases

ProteinModelPortaliQ5ZML1
SMRiQ5ZML1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000034376

Proteomic databases

PaxDbiQ5ZML1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000035021; ENSGALP00000034376; ENSGALG00000014426
GeneIDi418271
KEGGigga:418271

Organism-specific databases

CTDi3836

Phylogenomic databases

eggNOGiKOG0166 Eukaryota
COG5064 LUCA
GeneTreeiENSGT00760000119094
HOGENOMiHOG000167616
HOVERGENiHBG001846
InParanoidiQ5ZML1
KOiK15042
OMAiSWLLFVN
OrthoDBiEOG091G095Z
PhylomeDBiQ5ZML1
TreeFamiTF354205

Enzyme and pathway databases

ReactomeiR-GGA-211227 Activation of DNA fragmentation factor

Miscellaneous databases

PROiPR:Q5ZML1

Gene expression databases

BgeeiENSGALG00000014426 Expressed in 11 organ(s), highest expression level in skeletal muscle tissue

Family and domain databases

Gene3Di1.20.5.690, 1 hit
1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR032413 Arm_3
IPR000225 Armadillo
IPR002652 Importin-a_IBB
IPR036975 Importin-a_IBB_sf
IPR024931 Importing_su_alpha
PfamiView protein in Pfam
PF00514 Arm, 8 hits
PF16186 Arm_3, 1 hit
PF01749 IBB, 1 hit
PIRSFiPIRSF005673 Importin_alpha, 1 hit
SMARTiView protein in SMART
SM00185 ARM, 8 hits
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50176 ARM_REPEAT, 4 hits
PS51214 IBB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIMA5_CHICK
AccessioniPrimary (citable) accession number: Q5ZML1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: November 23, 2004
Last modified: October 10, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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