UniProtKB - Q5ZLV4 (NSUN2_CHICK)
RNA cytosine-C(5)-methyltransferase NSUN2
NSUN2
Functioni
RNA cytosine C5-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs). Involved in various processes, such as epidermal stem cell differentiation, testis differentiation and maternal to zygotic transition during early development: acts by increasing protein synthesis; cytosine C5-methylation promoting tRNA stability and preventing mRNA decay. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. tRNA methylation is required generation of RNA fragments derived from tRNAs (tRFs). Also mediates C5-methylation of mitochondrial tRNAs. Catalyzes cytosine C5-methylation of mRNAs, leading to stabilize them and prevent mRNA decay. Cytosine C5-methylation of mRNAs also regulates mRNA export. Also mediates cytosine C5-methylation of non-coding RNAs, such as vault RNAs (vtRNAs), promoting their processing into regulatory small RNAs. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.
By similarityCatalytic activityi
- cytidine48 in tRNA + S-adenosyl-L-methionine = 5-methylcytidine48 in tRNA + H+ + S-adenosyl-L-homocysteineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- cytidine49 in tRNA + S-adenosyl-L-methionine = 5-methylcytidine49 in tRNA + H+ + S-adenosyl-L-homocysteineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- cytidine50 in tRNA + S-adenosyl-L-methionine = 5-methylcytidine50 in tRNA + H+ + S-adenosyl-L-homocysteineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- cytidine34 in tRNA precursor + S-adenosyl-L-methionine = 5-methylcytidine34 in tRNA precursor + H+ + S-adenosyl-L-homocysteineBy similarityEC:2.1.1.203By similarityThis reaction proceeds in the forwardBy similarity direction.
- a cytidine in mRNA + S-adenosyl-L-methionine = a 5-methylcytidine in mRNA + H+ + S-adenosyl-L-homocysteineBy similarityThis reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 215 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 242 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 268 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Active sitei | 321 | NucleophilePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- methyltransferase activity Source: GO_Central
- mRNA (cytidine-5-)-methyltransferase activity Source: UniProtKB
- tRNA (cytosine-5-)-methyltransferase activity Source: UniProtKB
- tRNA binding Source: GO_Central
GO - Biological processi
- mRNA methylation Source: UniProtKB
- regulation of mRNA export from nucleus Source: UniProtKB
- regulation of stem cell differentiation Source: UniProtKB
- RNA methylation Source: GO_Central
- tRNA methylation Source: UniProtKB
- tRNA stabilization Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase, tRNA-binding |
Biological process | tRNA processing |
Ligand | S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: RNA cytosine-C(5)-methyltransferase NSUN2Curated (EC:2.1.1.-By similarity)Alternative name(s): |
Gene namesi | Name:NSUN2By similarity ORF Names:RCJMB04_4l11 |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Extracellular region or secreted
- extracellular exosome By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Nucleus
- nucleolus By similarity
Cytoskeleton
- spindle By similarity
Cytoskeleton
- spindle Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Mitochondrion
- mitochondrion Source: UniProtKB
Nucleus
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000289225 | 1 – 796 | RNA cytosine-C(5)-methyltransferase NSUN2Add BLAST | 796 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q5ZLV4 |
PRIDEi | Q5ZLV4 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 36 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 184 – 190 | S-adenosyl-L-methionine bindingPROSITE-ProRule annotation | 7 | |
Regioni | 435 – 501 | DisorderedSequence analysisAdd BLAST | 67 | |
Regioni | 707 – 796 | DisorderedSequence analysisAdd BLAST | 90 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 451 – 465 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 468 – 488 | Basic and acidic residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 707 – 756 | Basic and acidic residuesSequence analysisAdd BLAST | 50 | |
Compositional biasi | 775 – 796 | Polar residuesSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2198, Eukaryota |
InParanoidi | Q5ZLV4 |
OrthoDBi | 911098at2759 |
PhylomeDBi | Q5ZLV4 |
Family and domain databases
InterProi | View protein in InterPro IPR001678, MeTrfase_RsmB/NOP2 IPR023267, RCMT IPR023270, RCMT_NCL1 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR22808, PTHR22808, 1 hit |
Pfami | View protein in Pfam PF01189, Methyltr_RsmB-F, 1 hit |
PRINTSi | PR02008, RCMTFAMILY PR02011, RCMTNCL1 |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51686, SAM_MT_RSMB_NOP, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MGRRARDRRR QLQPQQRRER SGGGGGGGDD QAGWAGGYPE IVKENELFER
60 70 80 90 100
YYREQRIVPD GEWDAFMAAL REPLPATLRI TGYKSHAREI LHCLKEKYFR
110 120 130 140 150
ELQHLEVDGQ KVEMPQALSW YPEELAWHTN LSRKILRKSP QLERFHQFLV
160 170 180 190 200
SETECGNISR QEAVSMIPPL LLNVNPDHKI LDMCAAPGSK TAQLIEMLHA
210 220 230 240 250
DMNVPFPKGF VIANDVDNKR CYLLVHQAKR LNSPCIMVVN HDASSIPNLQ
260 270 280 290 300
VDVDGRKETL FYDRILCDVP CSGDGTMRKN IDVWKKWTTQ NSLQLHGLQL
310 320 330 340 350
RIATRGVEQL AEGGRMVYST CSLNPIENEA VIASLLEKSQ GALELADVSS
360 370 380 390 400
ELPGLKRMPG ITKWKVMLKD GQWFEEWKDV PSNRQTQIRP TMFPIKEEEK
410 420 430 440 450
LKAMNLERCI RILPHHQNTG GFFVAVLIKK SPMPWNKRQP KVHQKLPGKT
460 470 480 490 500
EDTEVTATNA GDGSEDATEK PTLAEDEEPK KVQELQNSDT EQSKKGVCGP
510 520 530 540 550
PPSKKMKLFG FKEDPFVFLP EDDPLFLPIQ KFYALDPSFP KMNLLTRTQE
560 570 580 590 600
GKKRQLYMVS KELRNVLLNN SEKMKVINTG IKVWSRNSDG EQFGCAFRLA
610 620 630 640 650
QEGIYTLYPF IHARIVNVCI EDVKILLTQE NPFLSKFSSE TQRKVKDMAM
660 670 680 690 700
GSIVLKYEPD PEKPDDLQCP IVLCGWQGKT SLRAFVPKNE RLHYLRMMGV
710 720 730 740 750
EVFKAKRKEG ESEGKTEEEV QCRPAQTEEG MDVEDKERDA VTKMEAEIDE
760 770 780 790
ESPRSPVESS AMEIENKSED SDQCSKNTNS HINQESKDMN TNNVKD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ719630 mRNA Translation: CAG31289.1 |
RefSeqi | NP_001026175.1, NM_001031004.1 |
Genome annotation databases
GeneIDi | 420938 |
KEGGi | gga:420938 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ719630 mRNA Translation: CAG31289.1 |
RefSeqi | NP_001026175.1, NM_001031004.1 |
3D structure databases
SMRi | Q5ZLV4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000021294 |
Proteomic databases
PaxDbi | Q5ZLV4 |
PRIDEi | Q5ZLV4 |
Genome annotation databases
GeneIDi | 420938 |
KEGGi | gga:420938 |
Organism-specific databases
CTDi | 54888 |
Phylogenomic databases
eggNOGi | KOG2198, Eukaryota |
InParanoidi | Q5ZLV4 |
OrthoDBi | 911098at2759 |
PhylomeDBi | Q5ZLV4 |
Miscellaneous databases
PROi | PR:Q5ZLV4 |
Family and domain databases
InterProi | View protein in InterPro IPR001678, MeTrfase_RsmB/NOP2 IPR023267, RCMT IPR023270, RCMT_NCL1 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR22808, PTHR22808, 1 hit |
Pfami | View protein in Pfam PF01189, Methyltr_RsmB-F, 1 hit |
PRINTSi | PR02008, RCMTFAMILY PR02011, RCMTNCL1 |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51686, SAM_MT_RSMB_NOP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NSUN2_CHICK | |
Accessioni | Q5ZLV4Primary (citable) accession number: Q5ZLV4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | November 23, 2004 | |
Last modified: | February 23, 2022 | |
This is version 80 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families